Interplay between allostery and intrinsic disorder in an ensemble.

abstract：:Many Zn(II)2Cys6 transcriptional regulators exhibit changes in phosphorylation that are coincident with their roles in transcriptional activation. It is, however, unclear whether these changes occur as a cause of, or as a result of, transcriptional activation. In this paper, we explore the relationship between these t...

journal_title：Biochemical Society transactions

authors： Leverentz MK,Reece RJ

更新日期：2006-11-01 00:00:00

abstract：:PLUNC (palate, lung and nasal epithelium clone) proteins make up the largest branch of the BPI (bactericidal/permeability-increasing protein)/LBP (lipopolysaccharide-binding protein) family of lipid-transfer proteins. PLUNCs make up one of the most rapidly evolving mammalian protein families and exhibit low levels of ...

journal_title：Biochemical Society transactions

authors： Bingle CD,Bingle L,Craven CJ

更新日期：2011-08-01 00:00:00

abstract：:Using the hyperthermophile Pyrococcus furiosus, we have delineated several key steps in CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated) invader defence pathways. P. furiosus has seven transcriptionally active CRISPR loci that together encode a total of 200 crRNAs (CRISPR RNAs...

journal_title：Biochemical Society transactions

authors： Terns RM,Terns MP

更新日期：2013-12-01 00:00:00

abstract：:The discovery that nuclear factor erythroid 2-related factor 2 (Nrf2) undergoes translocational oscillations from cytoplasm to nucleus in human cells with frequency modulation linked to activation of a stress-stimulated cytoprotective response raises the prospect that the Nrf2 works mechanistically analogous to a wire...

journal_title：Biochemical Society transactions

authors： Xue M,Momiji H,Rabbani N,Bretschneider T,Rand DA,Thornalley PJ

更新日期：2015-08-01 00:00:00

abstract：:miRNAs (microRNAs) are short non-coding RNAs that regulate gene expression post-transcriptionally. They generally bind to the 3'-UTR (untranslated region) of their target mRNAs and repress protein production by destabilizing the mRNA and translational silencing. The exact mechanism of miRNA-mediated translational repr...

journal_title：Biochemical Society transactions

authors： Cannell IG,Kong YW,Bushell M

更新日期：2008-12-01 00:00:00

abstract：:AWAP IV constitutes the C-terminal domain of the larger 81 kDa protein AWAK [Avian WAP (whey acidic protein) domain- and Kunitz domain-containing], which is predicted, through conserved domain database searching, to contain at least four WAP domains and one Kunitz domain. RT (reverse transcription)-PCR analyses reveal...

journal_title：Biochemical Society transactions

authors： Townes CL,Milona P,Hall J

更新日期：2006-04-01 00:00:00

abstract：:Mutations in MYO7A (myosin VIIa) cause Usher syndrome type 1B, a disorder involving profound congenital deafness and progressive blindness. In the retina, most MYO7A is localized in the apical region of the RPE (retinal pigmented epithelial) cells, and a small amount is associated with the ciliary and periciliary memb...

journal_title：Biochemical Society transactions

authors： Williams DS,Lopes VS

更新日期：2011-10-01 00:00:00

abstract：:The Kelch-like ECH associated protein 1 (Keap1)-NF-E2 p45-related factor 2 (Nrf2) pathway regulates networks of proteins that protect against the cumulative damage of oxidants, electrophiles and misfolded proteins. The interaction between transcription factor Nrf2 and its main negative cytoplasmic regulator Keap1 foll...

journal_title：Biochemical Society transactions

authors： Dinkova-Kostova AT,Baird L,Holmström KM,Meyer CJ,Abramov AY

更新日期：2015-08-01 00:00:00

abstract：:Isolation and purification of the [NiFe] hydrogenase of Desulfovibrio vulgaris Miyazaki F under aerobic conditions leads to a mixture of two states, Ni-A (unready) and Ni-B (ready). The two states are distinguished by different activation times and different EPR spectra. HYSCORE and ENDOR data and DFT calculations sho...

journal_title：Biochemical Society transactions

authors： van Gastel M,Fichtner C,Neese F,Lubitz W

更新日期：2005-02-01 00:00:00

abstract：:The cell-cycle-co-ordinated initiation of chromosomal replication is highly regulated. The ordered assembly and conformational change of specific proteins at the replication origin are crucial to the process of replication initiation. In Escherichia coli, ATP-DnaA molecules form multimeric complexes with the chromosom...

journal_title：Biochemical Society transactions

authors： Katayama T

更新日期：2008-02-01 00:00:00

abstract：:The statins, simvastatin and atorvastatin are the most widely prescribed drugs. Statins lower cholesterol levels through their action on HMG-CoA (3-hydroxy-3-methylglutaryl-CoA) reductase, an essential enzyme for the biosynthesis of cholesterol. Fungal HMG-CoA reductases are also inhibited by statins, resulting in red...

journal_title：Biochemical Society transactions

authors： Wikhe K,Westermeyer C,Macreadie IG

更新日期：2007-12-01 00:00:00

abstract：:P-type ATPases transport ions across biological membranes against concentration gradients and are essential for all cells. They use the energy from ATP hydrolysis to propel large intramolecular movements, which drive vectorial transport of ions. Tight coordination of the motions of the pump is required to couple the t...

journal_title：Biochemical Society transactions

authors： Dyla M,Basse Hansen S,Nissen P,Kjaergaard M

更新日期：2019-10-31 00:00:00

abstract：:Large dense-core vesicles (LDCVs) were labelled in cultured bovine adrenal chromaffin cells expressing fluorescent chimaeric 'cargo' proteins that were targeted to these secretory vesicles. When the cells were stimulated with nicotine 48 h after transduction, the fractional loss of fluorescent LDCVs was much greater t...

journal_title：Biochemical Society transactions

authors： Wiegand UK,Duncan RR,Greaves J,Chow RH,Shipston MJ,Apps DK

更新日期：2003-08-01 00:00:00

abstract：:Crystal structures combined with biochemical data show that the delta-endotoxins from Bacillus thuringiensis are structurally poised towards large-scale, irreversible conformational changes that transform them from the soluble protein bound at the cell surface into a membrane-embedded form causing lysis of susceptible...

journal_title：Biochemical Society transactions

authors： Li J,Derbyshire DJ,Promdonkoy B,Ellar DJ

更新日期：2001-08-01 00:00:00

abstract：:Formin family proteins (formins) represent an evolutionary conserved protein family encoded in the genome of a wide range of eukaryotes. Formins are hallmarked by a formin homology 1 (FH1) domain juxtaposed to an FH2 domain whereby they control actin and microtubule dynamics. Not surprisingly, formins are best known a...

journal_title：Biochemical Society transactions

authors： Isogai T,Innocenti M

更新日期：2016-12-15 00:00:00

abstract：:Mass spectrometry (MS) provides an impressive array of information about the structure, function and interactions of proteins. In recent years, many new developments have been in the field of native MS and these exemplify a new coming of age of this field. In this mini review, we connect the latest methodological and ...

journal_title：Biochemical Society transactions

authors： Allison TM,Bechara C

更新日期：2019-02-28 00:00:00

abstract：:The free energy landscape can provide a quantitative description of folding dynamics, if determined as a function of an optimally chosen reaction coordinate. The profile together with the optimal coordinate allows one to directly determine such basic properties of folding dynamics as the configurations of the minima a...

journal_title：Biochemical Society transactions

authors： Banushkina PV,Krivov SV

更新日期：2015-04-01 00:00:00

abstract：:In this study the network of ATP-consuming and -producing reactions, interacting via the cytosolic ATP/ADP+P(i) system, was studied for the first time in an intact organ, the isolated perfused rat liver, using top-down metabolic-control analysis. Flux control in the metabolically resting state (only oxidative phosphor...

journal_title：Biochemical Society transactions

authors： Soboll S,Brown GC

更新日期：2000-02-01 00:00:00

abstract：:It is now clear that NSPs (neutrophil serine proteases), including elastase, Pr3 (proteinase 3) and CatG (cathepsin G) are major pathogenic determinants in chronic inflammatory disorders of the lungs. Two unglycosylated natural protease inhibitors, SLPI (secretory leucocyte protease inhibitor) and elafin, and its prec...

journal_title：Biochemical Society transactions

authors： Zani ML,Tanga A,Saidi A,Serrano H,Dallet-Choisy S,Baranger K,Moreau T

更新日期：2011-10-01 00:00:00

abstract：:USP7 (also called HAUSP) is a de-ubiquitinating enzyme recently identified as a key regulator of the p53-mdm2 pathway, which stabilizes both p53 and mdm2. We have discovered that the Epstein-Barr nuclear antigen 1 protein of Epstein-Barr virus binds with high affinity to USP7 and disrupts the USP7-p53 interaction. The...

journal_title：Biochemical Society transactions

authors： Holowaty MN,Frappier L

更新日期：2004-11-01 00:00:00

abstract：:The biosynthetic intermediate PRPP (phosphoribosylpyrophosphate) has a central role in cellular biochemistry since it links carbon and nitrogen metabolism. Its importance may be reflected in the fact that, in the Saccharomyces cerevisiae (yeast) genome, there are five unlinked genes, PRS1-PRS5, each of which is theore...

journal_title：Biochemical Society transactions

authors： Vavassori S,Wang K,Schweizer LM,Schweizer M

更新日期：2005-11-01 00:00:00

abstract：:Diverse aetiologies result in significant deviation from homoeostasis in the kidney, leading to CKD (chronic kidney disease). CKD progresses to end-stage renal disease principally as a result of renal fibrosis, although the molecular mechanisms underlying this fibrotic process are still poorly understood. miRNAs (micr...

journal_title：Biochemical Society transactions

authors： Jenkins RH,Martin J,Phillips AO,Bowen T,Fraser DJ

更新日期：2012-08-01 00:00:00

abstract：:Since the discovery of the essential role of the β-barrel assembly machinery (BAM) for the membrane insertion of outer membrane proteins (OMPs) that are unrelated in sequence, members of this universally conserved family dominate discussions on OMP assembly in bacteria, mitochondria and chloroplasts. However, several ...

journal_title：Biochemical Society transactions

authors： Huysmans GH

更新日期：2016-06-15 00:00:00

abstract：:Nitrogenase activity in Rhodospirillum rubrum is post-translationally regulated by DRAG (dinitrogenase reductase glycohydrolase) and DRAT (dinitrogenase reductase ADP-ribosylation transferase). When a sudden increase in fixed nitrogen concentration or energy depletion is sensed by the cells, DRAG is inactivated and DR...

journal_title：Biochemical Society transactions

authors： Wang H,Norén A

更新日期：2006-02-01 00:00:00

abstract：:The lack or excess of the protein tau can be deleterious for neurons. The absence of tau can result in retarded neurogenesis and neuronal differentiation, although adult mice deficient in tau are viable, probably because of the compensation of the loss of tau by other MAPs (microtubule-associated proteins). On the con...

journal_title：Biochemical Society transactions

authors： Avila J,de Barreda EG,Fuster-Matanzo A,Simón D,Llorens-Martín M,Engel T,Lucas JJ,Díaz-Hernández M,Hernández F

更新日期：2012-08-01 00:00:00

abstract：:Alkalithermophiles are an exciting subset of extremophilic organisms and represent extremophiles that are adapted to two extreme conditions, i.e. to a combination of alkaline and thermobiotic growth conditions. Among the anaerobic alkalithermophiles are representatives of both Bacteria and Archaea within a wide variet...

journal_title：Biochemical Society transactions

authors： Wiegel J,Kevbrin VV

更新日期：2004-04-01 00:00:00

abstract：:HD (Huntington's disease) is a late onset heritable neurodegenerative disorder that is characterized by neuronal dysfunction and death, particularly in the cerebral cortex and medium spiny neurons of the striatum. This is followed by progressive chorea, dementia and emotional dysfunction, eventually resulting in death...

journal_title：Biochemical Society transactions

authors： Bithell A,Johnson R,Buckley NJ

更新日期：2009-12-01 00:00:00

abstract：:Inorganic polyphosphate (polyP) accumulates in acidocalcisomes, acidic calcium stores that have been found from bacteria to human cells. Proton pumps, such as the vacuolar proton pyrophosphatase (V-H(+)-PPase or VP1), the vacuolar proton ATPase (V-H(+)-ATPase) or both, maintain their acidity. A vacuolar transporter ch...

journal_title：Biochemical Society transactions

authors： Lander N,Cordeiro C,Huang G,Docampo R

更新日期：2016-02-01 00:00:00

abstract：:Triple-negative breast cancer (TNBC) is a heterogeneous breast cancer subtype that lacks targeted therapy due to the absence of estrogen, progesterone, and HER2 receptors. Moreover, TNBC was shown to have a poor prognosis, since it involves aggressive phenotypes that confer significant hindrance to therapeutic treatme...

journal_title：Biochemical Society transactions

authors： Rajagopal T,Talluri S,Venkatabalasubramanian S,Dunna NR

更新日期：2020-12-01 00:00:00

abstract：:PI3K (phosphoinositide 3-kinase) is a key regulator of cell growth, metabolism and survival. The frequent activation of the PI3K pathway in cancer has stimulated widespread interest in identifying potent and selective inhibitors of PI3K isoforms. The present paper highlights recent progress in identifying such molecul...

journal_title：Biochemical Society transactions

authors： Knight ZA,Shokat KM

更新日期：2007-04-01 00:00:00