Abstract:
:Endometrial cancer is one of the most common gynecologic malignancies and many factors influence in its growth and development. As in many other types of cancer, prostaglandin E(2) (PGE(2)) is thought to be an accelerator of cell proliferation and endometrial cancer progression. In this study, we examined the effect of FuEP2/Ex2, a soluble decoy receptor for PGE(2) on growth of endometrial cancer cells. A stable transfectant expressing FuEP2/Ex2 was established from human endometrial cancer Ishikawa cells (Ish-FuEP2/Ex2). Ish-FuEP2/Ex2 cells expressed FuEP2/Ex2 mRNA and protein. Expression levels of E-prostanoid receptor 1 (EP1), EP2, EP3, EP4, and F-prostanoid receptor (FP) were almost the same in Ish-FuEP2/Ex2 and vector control cells. Growth rates of Ish-FuEP2/Ex2 under normal culture conditions were also similar to vector control cells, although PGE(2)-induced growth stimulation was completely inhibited in Ish-FuEP2/Ex2 or by Ish-FuEP2/Ex2 culture medium. Moreover, phosphorylation of extracellular signal-regulated kinase (ERK) and induction of cyclooxygenase-2 (COX-2), vascular endothelial growth factor (VEGF), cyclin D1, and c-fos mRNA by PGE(2) were not observed in Ish-FuEP2/Ex2 and Ish-FuEP2/Ex2 culture medium-treated vector control cells, although they were found when treated with prostaglandin F(2α). An orthotopic xenograft model in athymic nude mice revealed that Ish-FuEP2/Ex2-injected mice had significantly decreased mean tumor area. The proportion of Ki-67-positive cells in the tumor lesion was also significantly lower in Ish-FuEP2/Ex2-injected mice. These findings suggest that an EP-targeting strategy using FuEP2/Ex2 may be of use in the treatment of endometrial cancer.
journal_name
Cancer Lettjournal_title
Cancer lettersauthors
Takahashi T,Ogawa H,Izumi K,Uehara Hdoi
10.1016/j.canlet.2011.02.033subject
Has Abstractpub_date
2011-07-01 00:00:00pages
67-75issue
1eissn
0304-3835issn
1872-7980pii
S0304-3835(11)00126-1journal_volume
306pub_type
杂志文章相关文献
CANCER LETTERS文献大全abstract::Stress conditions induced by routine treatments might affect cancer-associated fibroblasts in lung adenocarcinoma. The present study tried to explore transcriptome changes in lung fibroblasts under chemotherapeutics, irradiation, and hypoxia, which were induced by chemotherapy, radiotherapy, and anti-angiogenesis ther...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2020.10.032
更新日期:2021-01-28 00:00:00
abstract::Primary cultures of mouse embryo cells are more efficient in excising DNA-carcinogen adducts resulting from exposure to either 7-bromomethylbenz-[alpha]anthracene or the more carcinogen 7-bromomethyl-12-methylbenz[alpha]anthracene than are mouse L 929 cell suspension cultures. However, within each of these systems, th...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(79)80103-2
更新日期:1979-07-01 00:00:00
abstract::The NM23 family proteins are involved in a variety of biological processes including tumor metastasis, development, and differentiation; however, their functions in the regulation of cellular proliferation are poorly understood. We have investigated the role of one NM23 family protein, NM23H2, in the regulation of cel...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2008.10.018
更新日期:2009-03-18 00:00:00
abstract::Sulfasalazine (SSZ) is an anti-inflammatory drug that has been demonstrated to induce apoptosis and tumor regression through inhibition of plasma membrane cystine transporter xc(-). Cysteine is a rate-limiting precursor for intracellular glutathione (GSH) synthesis, which is vital for compound detoxification and maint...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2015.07.031
更新日期:2015-11-01 00:00:00
abstract::The effect of bile acids on the formation of azoxymethane induced aberrant crypt foci (ACF) was investigated using the fecal stream-excluded colons of colostomized F344 rats. The excluded colon was irrigated with saline or bile acids (1 mg/0.5 ml per day, 5 days/week) for 4 weeks. The mean numbers of ACF per colon in ...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(01)00525-0
更新日期:2001-08-28 00:00:00
abstract::The insulin-like growth factor (IGF) family of ligands, binding proteins and receptors is an important growth factor system involved in both the development of the organism and the maintenance of normal function of many cells of the body. The system also has powerful anti-apoptotic effects. More recently, evidence has...
journal_title:Cancer letters
pub_type: 杂志文章,评审
doi:10.1016/s0304-3835(03)00159-9
更新日期:2003-06-10 00:00:00
abstract::Eighty samples of cervical invasive squamous cell carcinoma were examined for ras gene mutations using polymerase chain reaction (PCR) followed by restriction enzyme digestion. We found 28 (35%) cervical cancers contained ras mutations at H-ras codon 12, 49 (61%) at K-ras codon 12, and 5 (6%) at K-ras codon 13. There ...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(95)03857-s
更新日期:1995-08-16 00:00:00
abstract::Third generation bisphosphonates (BPs), including YM175 and YM529, are known to inhibit bone resorption. The aim of this study was to evaluate the anti-tumor effects of these drugs on murine osteosarcoma cell lines, in terms of proliferation and apoptosis. We found that both YM175 and YM529 strongly inhibited the in v...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2005.06.041
更新日期:2006-07-08 00:00:00
abstract::Lysophosphatidic acid (LPA) receptors including LPA(1), LPA(2), and LPA(3) mediate lysophosphatidic acid signals. We analyzed the expression of LPA receptors, vascular endothelial growth factor (VEGF), and interleukin-8 in 97 patients from normal ovary to ovarian cancer, using reverse transcription polymerase chain re...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(02)00713-9
更新日期:2003-03-31 00:00:00
abstract::The anti-carcinogenic activity of dietary fruit and vegetables observed in several epidemiological and experimental animal studies is likely to be an effect of the combined exposure to a large number of substances acting together. This is plausible, as these compounds are present simultaneously in a diet containing ve...
journal_title:Cancer letters
pub_type: 杂志文章,评审
doi:10.1016/s0304-3835(97)04681-8
更新日期:1997-03-19 00:00:00
abstract::Tumor-associated macrophages (TAMs) have important roles in the angiogenesis and tumor immunosuppression of various cancers, including esophageal squamous cell carcinomas (ESCCs). To elucidate the roles of TAMs in ESCCs, we compared the gene expression profiles between human peripheral blood monocyte-derived macrophag...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2016.06.009
更新日期:2016-09-28 00:00:00
abstract::To investigate if genomic instability exists in tumors with cytogenetically normal karyotypes, we analyzed four diploid cancer cell lines A204, CAL51, CH1 and SK-UT-1B. We detected subtle genomic changes in all four cell lines. More of these alterations were found in A204 and CH1 than in the microsatellite unstable li...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2008.03.003
更新日期:2008-08-18 00:00:00
abstract::Soil samples were collected from the ground under the plants of Euphorbiaceae and Thymelaeaceae known to possess Epstein-Barr virus-activating diterpene esters. In a test system, the ether extracts of such soil samples at a concentration of 20 micrograms/ml induced Epstein-Barr virus early antigen in approximately 5-2...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(83)90144-1
更新日期:1983-06-01 00:00:00
abstract::B7-homolog 4 (B7-H4), a recently identified homolog of B7.1/2 (CD80/86), has been described to exert co-stimulatory and immune regulatory functions. We investigated the expression and the functional activity of B7-H4 in lung cancer in vitro and in vivo. Although a lung cancer cell line constitutively expressed B7-H4 m...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2011.11.017
更新日期:2012-04-01 00:00:00
abstract::Lectins are suitable tools for investigating the glycoconjugate characteristics of metastatic cells. In the present study, we investigated whether there were differences between high metastatic T3 cells and a low metastatic isolate in their reactivities to several lectins specific for galactosyl and sialyl groups. Ana...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(91)90177-j
更新日期:1991-08-01 00:00:00
abstract::Calcium channel blockers of 3 chemical classes and calmodulin antagonists have recently been shown to enhance the cytotoxicity of conventional cancer chemotherapeutic agents. We tested the ability of nifedipine, a dihydropyridine class calcium channel blocker, to enhance the cytotoxicity of cisplatin, the current chem...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(86)90087-x
更新日期:1986-02-01 00:00:00
abstract::Stromal factors play a critical role in the development of the mammary gland. Using a three dimensional-coculture model we demonstrate a significant role for stromal fibroblasts in the regulation of normal mammary epithelial morphogenesis and the control of tumor growth. Both soluble factors secreted by fibroblasts an...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2012.06.014
更新日期:2012-12-28 00:00:00
abstract::We investigated the molecular mechanisms responsible for fisetin-induced apoptosis in U266 cells. Fisetin elicited the cytotoxicity in U266 cells, manifested as an increased fraction of the cells with sub-G1 content or stained positively with TUNEL labeling. Fisetin enhanced caspase-3 activation, downregulation of Bcl...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2012.01.008
更新日期:2012-06-28 00:00:00
abstract::The immunomodulatory and antimetastatic/antitumor activity of thymosin alpha(1) (Talpha(1)) was evaluated in BALB/c-mice. Daily subcutaneous application (7 consecutive days, 0.01-10 microg of Talpha(1)/injection per mouse) upregulated the number of thymocytes and peripheral blood cells in tumor bearing mice. To check ...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(00)00510-3
更新日期:2000-10-16 00:00:00
abstract::We investigated the molecular mechanisms involved in the anti-proliferative activity exerted by conjugated linoleic acid (CLA) on the estrogen unresponsive MDA-MB-231 human breast cancer cell line. The effects on cell proliferation, cell cycle progression and induction of apoptosis were examined. CLA caused the reduct...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2005.03.029
更新日期:2006-03-28 00:00:00
abstract::Sulforaphane (SFN) is an isothiocyanate found in cruciferous vegetables, with particularly high levels detected in broccoli and broccoli sprouts. Over a decade ago, this phytochemical was identified as a likely chemopreventive agent based on its ability to induce Phase 2 detoxification enzymes, as well as to inhibit P...
journal_title:Cancer letters
pub_type: 杂志文章,评审
doi:10.1016/j.canlet.2005.02.033
更新日期:2006-02-28 00:00:00
abstract::The p53 signaling pathway plays critical roles in determining the radio-sensitivity of normal tissues but is frequently inactivated in cancer. In various cancer cells, p53 has different effects on radio-sensitivity through complex mechanisms. Acetylation of p53 is an important means of post-translational modifications...
journal_title:Cancer letters
pub_type: 杂志文章,评审
doi:10.1016/j.canlet.2015.04.015
更新日期:2015-07-28 00:00:00
abstract::New, potentially tumor-specific antigens have been described in Bcr/Abl positive leukemias. Besides the main BCR/ABL hybrid fusion transcripts, a small number of transcripts derived from alternative splicing between BCR exons 1, 13, and 14 with ABL exons 4 and 5 have been identified. These variants are expressed in ch...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2008.10.032
更新日期:2009-04-08 00:00:00
abstract::Viscum album L. (VAL) is a phytopreparation used in adjuvant cancer therapy with both immunostimulatory and DNA stabilizing properties at low drug concentrations and cytostatic/cytotoxic properties at higher concentrations. The present work examines the cytotoxic effects of VAL extracts produced from mistletoes grown ...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(95)04038-2
更新日期:1996-01-19 00:00:00
abstract::Receptors for all classes of steroid hormones were identified in cytosols of adenocarcinoma of the lung. In contrast, the incidence of receptor in squamous cell carcinoma was limited and small cell carcinomas appeared devoid of receptor. Receptor binding was of high affinity, saturable and localized to the 8, 6-7 and ...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(82)90014-3
更新日期:1982-09-01 00:00:00
abstract::We previously reported that prostaglandin E(2) contributes to colon carcinogenesis through its binding to the prostaglandin E receptor subtype EP(1) using a genetic approach in EP(1)-knockout mice and a pharmacological approach with the EP(1) selective antagonist, ONO-8711. In the present study, we examined the effect...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(00)00440-7
更新日期:2000-08-01 00:00:00
abstract::Tumor-derived exosomes (TEXs) contain enriched miRNAs that act as novel non-invasive biomarkers for cancer diagnosis and play a role in cancer progression. We investigated the exosomal miRNAs that affect cancer progression in non-small cell lung cancer (NSCLC) and identified the specific molecules involved. We identif...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2020.01.023
更新日期:2020-04-10 00:00:00
abstract::In the current study we examined the regulation of Bak, a death promoter of an apoptotic pathway, in the human breast cancer cell line MCF-7. We observed a time-dependent increase in both Bak mRNA and protein levels which appeared to correlate well with the increase in cell density. We also found that treatment of cel...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(97)00430-8
更新日期:1998-02-13 00:00:00
abstract::The three mistletoe (Viscum album L.) lectins. ML I, ML II and ML III, were tested on their ability to enhance the secretion of the cytokines tumor necrosis factor (TNF)alpha, interleukin (IL)-1 alpha, IL-1 beta and IL-6 by human monocytes obtained from healthy donors. At lectin concentrations from 0.02 to 20/pg ml (1...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(96)04401-1
更新日期:1996-12-03 00:00:00
abstract::The desensitization mechanism of the EGF receptor (EGFR) is important for the regulation of cancer cells. Although the phosphorylation of EGFR at Tyr1045 and Ser1046/1047 (Ser1046/7) reportedly accounts for such desensitization, the precise mechanism still remains unknown. Therefore, the present study investigated the...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2008.11.034
更新日期:2009-05-08 00:00:00