Abstract:
:DEAD-box proteins disrupt or remodel RNA and protein/RNA complexes at the expense of ATP. The catalytic core is composed of two flexibly connected RecA-like domains. The N-terminal domain contains most of the motifs involved in nucleotide binding and serves as a minimalistic model for helicase/nucleotide interactions. A single conserved glutamine in the so-called Q-motif has been suggested as a conformational sensor for the nucleotide state. To reprogram the Thermus thermophilus RNA helicase Hera for use of oxo-ATP instead of ATP and to investigate the sensor function of the Q-motif, we analyzed helicase activity of Hera Q28E. Crystal structures of the Hera N-terminal domain Q28E mutant (TthDEAD_Q28E) in apo- and ligand-bound forms show that Q28E does change specificity from adenine to 8-oxoadenine. However, significant structural changes accompany the Q28E mutation, which prevent the P-loop from adopting its catalytically active conformation and explain the lack of helicase activity of Hera_Q28E with either ATP or 8-oxo-ATP as energy sources. 8-Oxo-adenosine, 8-oxo-AMP, and 8-oxo-ADP weakly bind to TthDEAD_Q28E but in non-canonical modes. These results indicate that the Q-motif not only senses the nucleotide state of the helicase but could also stabilize a catalytically competent conformation of the P-loop and other helicase signature motifs.
journal_name
Biol Chemjournal_title
Biological chemistryauthors
Strohmeier J,Hertel I,Diederichsen U,Rudolph MG,Klostermeier Ddoi
10.1515/BC.2011.034subject
Has Abstractpub_date
2011-04-01 00:00:00pages
357-69issue
4eissn
1431-6730issn
1437-4315journal_volume
392pub_type
杂志文章abstract::The kallikrein-kinin and renin-angiotensin (KKS-RAS) systems represent two highly regulated proteolytic systems that are involved in several physiological and pathological processes. Although their protein-protein interactions can be studied using experimental approaches, it is difficult to differentiate between direc...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2010.046
更新日期:2010-04-01 00:00:00
abstract::While the role of C2-ceramide in the induction of programmed cell death (PCD) in animal systems has been well documented, little is known of its role in plant cells. Here we show that C2-ceramide induces PCD in Arabidopsis suspension cultures, which is preceded by the generation of a calcium transient and an increase ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2005.020
更新日期:2005-02-01 00:00:00
abstract::The N-terminal propeptide domains of several cathepsin L-like cysteine proteases have been shown to possess potent inhibitory activity. Here we report the first kinetic characterisation of the inhibition properties of the cathepsin V propeptide (CatV PP). Using a facile recombinant approach we demonstrate expression, ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2007.053
更新日期:2007-05-01 00:00:00
abstract::Metarhizium anisopliae is an entomopathogenic fungus relevant in biotechnology with applications like malaria vector control. Studies of its virulence factors are therefore of great interest. Fungal ribotoxins are toxic ribonucleases with extraordinary efficiency against ribosomes and suggested as potential insecticid...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2016-0119
更新日期:2017-01-01 00:00:00
abstract::Xeroderma pigmentosum complementation group D protein (XPD) is an iron-sulfur cluster containing 5'-3' helicase and, in humans, part of the transcription factor TFIIH. TFIIH is involved in nucleotide excision repair as well as in transcription initiation. Recently, three different groups have reported the structures o...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2010.076
更新日期:2010-07-01 00:00:00
abstract::N.BstNBI is a unique restriction endonuclease isolated from Bacillus stearothermophilus. We have characterized the recognition sequence and the cleavage site of N.BstNBI. Mapping of cleavage sites of N.BstNBI showed that it recognizes an asymmetric sequence, 5' GAGTC 3', and cleaves only on the top strand 4 base pairs...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2000.137
更新日期:2000-11-01 00:00:00
abstract::Eukaryotic organisms have evolved complex and robust cellular stress response pathways to ensure maintenance of proteostasis and survival during fluctuating environmental conditions. Highly conserved stress response pathways can be triggered and coordinated at the cell-autonomous and cell-nonautonomous level by proteo...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2019-0385
更新日期:2020-08-27 00:00:00
abstract::The kallikrein-related peptidase (KLK) gene family of 15 serine proteases encodes many proteins, including prostate specific antigen (PSA or KLK3), that are well described and/or are potential biomarkers for hormone-related cancers. Variant mRNA transcripts produced by alternative splicing, polyadenylation or AUG site...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2006.088
更新日期:2006-06-01 00:00:00
abstract::Drug-induced phospholipidosis is a lysosomal storage disorder characterized by excessive accumulation of phospholipids. Its cellular mechanism is still not well understood, but it is known that cationic amphiphilic drugs can induce it. These drugs have a hydrophilic amine head group that can be protonated in the endol...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2019-0270
更新日期:2019-12-18 00:00:00
abstract::To elucidate the structures and dynamics of membrane proteins, highly advanced biophysical methods have been developed that often require significant resources, both for sample preparation and experimental analyses. For very complex systems, such as membrane transporters, ion channels or G-protein coupled receptors (G...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2018-0473
更新日期:2019-09-25 00:00:00
abstract::An inert polysaccharide scaffold identified as a 5-15% component of prion rods (PrP 27-30) is unambiguously distinguishable from the N-glycosyl groups and the GPI anchor of PrP, and consists predominantly of 1,4-linked glucose with some branching via 1,4,6-linked glucose. We show that this polysaccharide scaffold is a...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2005.131
更新日期:2005-11-01 00:00:00
abstract::The lysosomal cysteine proteases cathepsin B and L play important roles in tumor cell invasion. An imbalance between these cathepsins and their endogenous inhibitors, the cystatins, has been associated with development of the metastatic phenotype. Accordingly, many studies have indicated potential use of cystatins in ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2008.035
更新日期:2008-04-01 00:00:00
abstract::Bradykinin causes vascular relaxations through release of endothelial relaxing factors including prostacyclin, nitric oxide (NO) and epoxyeicosatrienoic acids (EETs). Bradykinin is metabolized by angiotensin converting enzyme (ACE) and ACE inhibition enhances bradykinin relaxations. Our goal was to characterize the ro...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2012-0348
更新日期:2013-09-01 00:00:00
abstract::We have previously described Kunitz-type serine proteinase inhibitors purified from Bauhinia seeds. Human plasma kallikrein shows different susceptibility to those inhibitors. In this communication, we describe the interaction of human plasma kallikrein with fluorogenic and non-fluorogenic peptides based on the Bauhin...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2001.016
更新日期:2001-01-01 00:00:00
abstract::Magnesium cation (Mg2+) is the most abundant divalent cation in living cells, where it is required for various intracellular functions. In chloroplasts and cyanobacteria, established photosynthetic model systems, Mg2+ is the central ion in chlorophylls, and Mg2+ flux across the thylakoid membrane is required for count...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2018-0476
更新日期:2019-09-25 00:00:00
abstract::The influence of the cellular milieu, a complex and crowded solvent, is often neglected when biomolecular structure and function are studied in vitro. To mimic the cellular environment, crowding effects are commonly induced in vitro using artificial crowding agents like Ficoll or dextran. However, it is unclear if suc...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2015-0161
更新日期:2016-01-01 00:00:00
abstract::Five moeA mutants were generated by replacing some conserved amino acids of MoeA by site-directed mutagenesis. The mutants were assayed for the ability to restore in vivo nitrate reductase activity of the moeA mutant Escherichia coli JRG97 and in vitro Neurospora crassa nit-1 nitrate reductase activity. The replacemen...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2002.034
更新日期:2002-02-01 00:00:00
abstract::Bradykinin (BK) is a vasorelaxant, algesic and inflammatory agent. Angiotensin II (AngII) is known to control vascular tone and promote growth, inflammation and artherogenesis. There is evidence for cross talking between BK and AngII receptors. Therefore, the effect of lack of kinin receptors was assessed in mice with...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2009.081
更新日期:2009-09-01 00:00:00
abstract::Matrix metalloproteinases (MMPs) are a family of zinc endopeptidases that have been implicated in various disease processes. Different classes of MMP inhibitors, including hydroxamic acids, phosphinic acids and thiols, have been previously described. Most of these mimic peptides and most likely bind in a similar way t...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2001.159
更新日期:2001-08-01 00:00:00
abstract::Upon activation of the B cell antigen receptor (BCR), the spleen tyrosine kinase (Syk) and the Src family kinase Lyn phosphorylate tyrosines of the immunoreceptor tyrosine-based activation motif (ITAM) of Igα and Igβ which further serve as binding sites for the SH2 domains of these kinases. Using a synthetic biology a...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2018-0308
更新日期:2019-03-26 00:00:00
abstract::Interleukin-13 (IL-13) is a T-cell-derived pleiotropic cytokine of particular medical importance because of its critical role in the development of allergic asthma. The effects of IL-13 on its target cells are mediated through a dimeric transmembrane receptor (IL-13R), which shares the IL-4Ralpha subunit with the IL-4...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2007.036
更新日期:2007-03-01 00:00:00
abstract::MHC (major histocompatibility complex) class I molecules bind intracellular virus-derived peptides in the endoplasmic reticulum (ER) and present them at the cell surface to cytotoxic T lymphocytes. Peptide-free class I molecules at the cell surface, however, could lead to aberrant T cell killing. Therefore, cells ensu...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2004.100
更新日期:2004-09-01 00:00:00
abstract::M.EcoRV is an alpha-adenine DNA methyltransferase. According to structure predictions, the enzyme consists of a catalytic domain, which has a structure similar to all other DNA-methyltransferases, and a smaller DNA-recognition domain. We have investigated this enzyme by random mutagenesis, using error-prone PCR, follo...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/bchm.1998.379.4-5.475
更新日期:1998-04-01 00:00:00
abstract::The production and processing of ribosomal RNA is a complex and well-coordinated nucleolar process for ribosome biogenesis. Progress in understanding nucleolar structure and function has lead to the unexpected discovery of the nucleolus as a highly sensitive sensor of cellular stress and an important regulator of the ...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2013-0153
更新日期:2013-09-01 00:00:00
abstract::The p53 tumour suppressor gene is frequently mutated in human tumours and different tumour-derived mutations have varying effects on cells. The effect of a novel tumour-derived p53 mutation and two recently described mutations from South African breast cancer patients on the growth rate, colony formation, cell cycle a...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2008.010
更新日期:2008-01-01 00:00:00
abstract::The role of Zn2+-sensing receptor GPR39 on glucose homeostasis and incretin regulation was assessed in enteroendocrine L- and K-cells. Anti-hyperglycaemic, insulinotropic and incretin secreting properties of Zn2+ were explored in normal, diabetic and incretin receptor knockout mice. Compared to intraperitoneal injecti...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2018-0393
更新日期:2019-06-20 00:00:00
abstract::Synthetic biologists aim at engineering controllable biological parts such as DNA, RNA and proteins in order to steer biological activities using external inputs. Proteins can be controlled in several ways, for instance by regulating the expression of their encoding genes with small molecules or light. However, post-t...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2018-0309
更新日期:2019-03-26 00:00:00
abstract::Cells need to process multifaceted external cues to steer their dynamic behavior. To efficiently perform this task, cells implement several exploratory mechanisms to actively sample their environment. In particular, cells can use exploratory actin-based cell protrusions and contractions to engage and squeeze the envir...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2017-0341
更新日期:2018-07-26 00:00:00
abstract::Major advances have been made during the last decade in our understanding of adrenal steroid hormone biosynthesis. Two key players in these pathways are the human mitochondrial cytochrome P450 enzymes CYP11B1 and CYP11B2, which catalyze the final steps in the biosynthesis of cortisol and aldosterone. Using data from m...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2002.174
更新日期:2002-10-01 00:00:00
abstract::The adipocytokine adiponectin and its receptor (AdipoR) comprise a new receptor-ligand system that is involved in a variety of clinically important morbidities such as obesity, type 2 diabetes and cardiovascular diseases. Adiponectin exerts a multitude of beneficial and tissue specific effects depending on its unique,...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2010.104
更新日期:2010-09-01 00:00:00