Tapasin and other chaperones: models of the MHC class I loading complex.

Abstract:

:MHC (major histocompatibility complex) class I molecules bind intracellular virus-derived peptides in the endoplasmic reticulum (ER) and present them at the cell surface to cytotoxic T lymphocytes. Peptide-free class I molecules at the cell surface, however, could lead to aberrant T cell killing. Therefore, cells ensure that class I molecules bind high-affinity ligand peptides in the ER, and restrict the export of empty class I molecules to the Golgi apparatus. For both of these safeguard mechanisms, the MHC class I loading complex (which consists of the peptide transporter TAP, the chaperones tapasin and calreticulin, and the protein disulfide isomerase ERp57) plays a central role. This article reviews the actions of accessory proteins in the biogenesis of class I molecules, specifically the functions of the loading complex in high-affinity peptide binding and localization of class I molecules, and the known connections between these two regulatory mechanisms. It introduces new models for the mode of action of tapasin, the role of the class I loading complex in peptide editing, and the intracellular localization of class I molecules.

journal_name

Biol Chem

journal_title

Biological chemistry

authors

Wright CA,Kozik P,Zacharias M,Springer S

doi

10.1515/BC.2004.100

keywords:

subject

Has Abstract

pub_date

2004-09-01 00:00:00

pages

763-78

issue

9

eissn

1431-6730

issn

1437-4315

journal_volume

385

pub_type

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