Abstract:
:The influence of the cellular milieu, a complex and crowded solvent, is often neglected when biomolecular structure and function are studied in vitro. To mimic the cellular environment, crowding effects are commonly induced in vitro using artificial crowding agents like Ficoll or dextran. However, it is unclear if such effects are also observed in cellulo. Diverging results on protein stability in living cells point out the need for new quantitative methods to investigate the contributions of excluded volume and nonspecific interactions to the cellular crowding effect. We show how new crowding sensitive probes may be utilized to directly investigate crowding effects in living cells. Moreover, we discuss processes where crowding effects could play a crucial role in molecular cell biology.
journal_name
Biol Chemjournal_title
Biological chemistryauthors
Gnutt D,Ebbinghaus Sdoi
10.1515/hsz-2015-0161subject
Has Abstractpub_date
2016-01-01 00:00:00pages
37-44issue
1eissn
1431-6730issn
1437-4315pii
/j/bchm.just-accepted/hsz-2015-0161/hsz-2015-0161.journal_volume
397pub_type
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