Abstract:
:Type I allergy, a hypersensitivity disease affecting almost 20% of the population worldwide, is based on the IgE recognition of otherwise harmless antigens (i.e., allergens). Allergen-induced crosslink of effector cell-bound IgE antibodies leads to the release of biological mediators and thus to immediate disease symptoms (allergic rhinitis, conjunctivitis and asthma). Specific immunotherapy, the only causative treatment of Type I allergy, is based on the administration of increasing doses of allergens to allergic patients in order to yield allergen-specific non-responsiveness. Major disadvantages are 1. that current forms of allergen immunotherapy are performed with allergens difficult to standardize which cannot be matched to the patients reactivity profile and 2. that the administration of active allergen preparations can cause anaphylactic side effects. Through the application of molecular biological techniques many relevant environmental allergens have been produced as active recombinant proteins which allow component-resolved allergy diagnosis and thus represent the basis for patient-tailored forms of immunotherapy. Here we review molecular strategies which have been recently applied to generate genetically engineered and synthetic hypoallergenic allergen derivatives for patient-tailored and safe vaccination against Type I allergy.
journal_name
Biol Chemjournal_title
Biological chemistryauthors
Valenta R,Vrtala S,Focke-Tejkl M,Bugajska-Schretter,Ball T,Twardosz A,Spitzauer S,Grönlund H,Kraft Ddoi
10.1515/BC.1999.101keywords:
subject
Has Abstractpub_date
1999-07-01 00:00:00pages
815-24issue
7-8eissn
1431-6730issn
1437-4315journal_volume
380pub_type
杂志文章,评审abstract::Major advances have been made during the last decade in our understanding of adrenal steroid hormone biosynthesis. Two key players in these pathways are the human mitochondrial cytochrome P450 enzymes CYP11B1 and CYP11B2, which catalyze the final steps in the biosynthesis of cortisol and aldosterone. Using data from m...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2002.174
更新日期:2002-10-01 00:00:00
abstract::Full-length Hsp70 protein (Hsp70) and the C-terminal domain of Hsp70 (Hsp70C) both stimulate the cytolytic activity of naive natural killer (NK) cells against Hsp70-positive tumor target cells. Here, we describe the characterization of Hsp70-NK cell interaction with binding studies using the human NK cell line YT. Bin...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2003.030
更新日期:2003-02-01 00:00:00
abstract::Here we report a detailed analysis of magnesium (Mg²+) ion effects on furin hydrolysis of fluorescent resonance energy transfer decapeptide substrates derived from canonical R-X-K/R-R furin cleavage motifs within certain viral envelope glycoproteins and eukaryotic proproteins. Using virus-derived sequences a selective...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2010.114
更新日期:2010-09-01 00:00:00
abstract::The surface topography of red blood cells (RBCs) was investigated under near-physiological conditions using atomic force microscopy (AFM). An immobilization protocol was established where RBCs are coupled via molecular bonds of the membrane glycoproteins to wheat germ agglutinin (WGA), which is covalently and flexibly...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2004.124
更新日期:2004-10-01 00:00:00
abstract::Protein transport, especially into different cellular compartments, is a highly coordinated and regulated process. The molecular machineries which carry out these transport processes are highly complex in structure, function, and regulation. In the case of chloroplasts, thousands of protein molecules have been estimat...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2009.051
更新日期:2009-08-01 00:00:00
abstract::The pancreatic β-cell surface protein Tmem27 is promotes the preservation of functional β-cell mass. It is a selective substrate of the protease Bace2, yet the intramolecular features of Tmem27 that regulate its processing by this sheddase have not been characterized. In particular, the importance of homodimerization,...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2012-0104
更新日期:2012-05-01 00:00:00
abstract::Major vault protein (MVP) is the predominant member of a large cytosolic ribonucleoprotein particle, termed vault. We have previously shown that MVP derived from electric ray electric organ becomes phosphorylated by protein kinase C in vitro and by tyrosine kinase in vivo. Here we show that MVP from two mammalian cell...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2001.180
更新日期:2001-10-01 00:00:00
abstract::Knockout mice are the gold standard to probe for the role of a specific protease within the interacting network of proteases, substrates, and inhibitors. This proteolytic network, or protease web, determines cell signaling and organ homeostasis. Therefore, protease deficiency or inhibition is intrinsically tied to alt...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2009.015
更新日期:2009-02-01 00:00:00
abstract::Interleukin-13 (IL-13) is a T-cell-derived pleiotropic cytokine of particular medical importance because of its critical role in the development of allergic asthma. The effects of IL-13 on its target cells are mediated through a dimeric transmembrane receptor (IL-13R), which shares the IL-4Ralpha subunit with the IL-4...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2007.036
更新日期:2007-03-01 00:00:00
abstract::Recent progress in the identification and partial characterization of novel genes encoding cysteine proteases of the papain family has considerably increased our knowledge of this family of enzymes. Kinetic data available to date for this large family indicate relatively broad, overlapping specificities for most enzym...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2003.094
更新日期:2003-06-01 00:00:00
abstract::Plasma prekallikrein (PPK) is synthesised in hepatocytes and secreted into the blood, where it participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. Recently we demonstrated by quantitative RT-PCR that the human PPK gene is transcribed not only in the l...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2007.104
更新日期:2007-09-01 00:00:00
abstract::We have previously shown that compatible organic osmolytes, such as betaine, myo-inositol and taurine, are part of the stress response of normal human keratinocytes (NHKs) to ultraviolet B (UVB) radiation. In this regard, we tested human HaCaT keratinocytes as a surrogate cell line for NHK. HaCaT cells osmo-dependentl...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2007.140
更新日期:2007-12-01 00:00:00
abstract::Hsp70 chaperone systems play an essential role in the life cycle of many proteins not only in an hostile environment but also under normal growth conditions. In the course of evolution the diversification of functions was accompanied by an amplification of components of the Hsp70 system. Here strategies are reviewed h...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:
更新日期:1998-03-01 00:00:00
abstract::A high-resolution crystallographic structure determination of a protein-ligand complex is generally accepted as the 'gold standard' for structure-based drug design, yet the relationship between structure and affinity is neither obvious nor straightforward. Here we analyze the interactions of a series of serine protein...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2014-0158
更新日期:2014-07-01 00:00:00
abstract::The lysosomal cysteine proteinase cathepsin B has been implicated in the progression of various human tumors including ovarian cancer. Included in this study were 63 patients with epithelial ovarian carcinoma. Follow-up information (median follow-up period 7 years) was available for all patients, among whom 42 (66.7%)...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2002.146
更新日期:2002-07-01 00:00:00
abstract::Mammalian apolipoproteinB (apoB) RNA editing is a site-specific deamination reaction that mediates the C to U conversion responsible for apoB48 production in the mammalian small intestine. This process is not detected in chicken apoB RNA. Mammalian apoB RNA editing is mediated by a multicomponent enzyme complex that i...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/bchm.1998.379.8-9.1075
更新日期:1998-08-01 00:00:00
abstract::Protein folding is one of the fundamental processes in life and therefore needs to be tightly regulated. Many cellular quality control systems are in place to ensure that proteostasis is optimally adjusted for a changing environment, facilitating protein folding, translocation and degradation. These systems include th...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2018-0458
更新日期:2019-06-26 00:00:00
abstract::Oxidative stress has for a long time been associated with cell death, especially classical necrosis, however, its role in other cell death pathways is less clear. Here, we evaluated in a comparative way, the effect of four different reactive oxygen species (ROS) scavengers, N-acetyl-L-cysteine (NAC), α-tocopherol and ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2017-0317
更新日期:2019-01-28 00:00:00
abstract::N.BstNBI is a unique restriction endonuclease isolated from Bacillus stearothermophilus. We have characterized the recognition sequence and the cleavage site of N.BstNBI. Mapping of cleavage sites of N.BstNBI showed that it recognizes an asymmetric sequence, 5' GAGTC 3', and cleaves only on the top strand 4 base pairs...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2000.137
更新日期:2000-11-01 00:00:00
abstract::Group II introns are autocatalytic RNAs which self-splice in vitro. However, in vivo additional protein factors might be involved in the splicing process. We used an affinity chromatography method called 'StreptoTag' to identify group II intron binding proteins from Saccharomyces cerevisiae. This method uses a hybrid ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2004.076
更新日期:2004-07-01 00:00:00
abstract::The main function of the epidermis is to establish a vital multifunctional barrier between the body and its external environment. A defective epidermal barrier is one of the key features of atopic dermatitis (AD), a chronic and relapsing inflammatory skin disorder that affects up to 20% of children and 2-3% of adults ...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2015-0141
更新日期:2015-11-01 00:00:00
abstract::The kinin B2 receptor, which is constitutively expressed in a large number of tissues, mediates most of the known effects of bradykinin (BK). Normally undetectable in healthy tissues, the B1 receptor is strongly over-expressed under pathological conditions. BK is an important mediator in renal homeostasis and is mainl...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2006.004
更新日期:2006-01-01 00:00:00
abstract::γ-Secretase is a membrane-embedded protease complex with presenilin as the catalytic component. Cleavage within the transmembrane domain of the amyloid β-protein precursor (APP) by γ-secretase produces the C-terminus of the amyloid β-peptide (Aβ), a proteolytic product prone to aggregation and strongly linked to Alzhe...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2012-0140
更新日期:2012-09-01 00:00:00
abstract::Nanoparticles (NPs) are recognized as an attractive vehicles for cancer treatment due to their targeted drug release. Gastric cancer is an important killer disease, and its therapy methods still need improvement. The NPs were prepared using a precipitation method, and were evaluated using transmission electron microsc...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2017-0208
更新日期:2018-02-23 00:00:00
abstract::Latent episomal genomes of Epstein-Barr virus, a human gammaherpesvirus, represent a suitable model system for studying replication and methylation of chromosomal DNA in mammals. We analyzed the methylation patterns of CpG dinucleotides in the latent origin of DNA replication of Epstein-Barr virus using automated fluo...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2000.014
更新日期:2000-02-01 00:00:00
abstract::Human inositol phosphate multikinase (IPMK) is a multifunctional protein in cellular signal transduction, namely, a multispecific inositol phosphate kinase, phosphatidylinositol 3-kinase, and a scaffold within the mTOR-raptor complex. To fulfill these nuclear and cytoplasmic functions, intracellular targeting of IPMK ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2011-0209
更新日期:2012-03-01 00:00:00
abstract::Transforming growth factor-beta1 (TGF-beta1) has a biphasic effect on the growth of renal epithelial cells. In transformed cells, TGF-beta1 appears to accelerate the proliferation of malignant cells. The diverse cellular functions of TGF-beta1 are regulated by three high-affinity serine/threonine kinase receptors, nam...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2005.045
更新日期:2005-04-01 00:00:00
abstract::In a recent study we showed that two proteinases (CMS2MS2 and CMS2MS3) from Carica candamarcensis enhance mammalian cell proliferation. The aim of the present study is the determination of the primary structure of CMS2MS2 and prediction of its three-dimensional structure. The protein contains 214 residues, including t...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2007.083
更新日期:2007-08-01 00:00:00
abstract::Cellular volume regulation is fundamental for numerous physiological processes. The volume-regulated anion channel, VRAC, plays a crucial role in regulatory volume decrease. This channel, which is ubiquitously expressed in vertebrates, has been vastly characterized by electrophysiological means. It opens upon cell swe...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2015-0127
更新日期:2015-09-01 00:00:00
abstract::The saline extract of Bauhinia bauhinioides dry seeds was shown to inhibit cruzipain, a cysteine proteinase from Trypanosoma cruzi. The inhibitory activity was assigned to a protein with 164 amino acid residues and molecular mass of 18 034 Da that was purified by chromatography on DEAE-Sephadex, trypsin-Sepharose (rem...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2001.103
更新日期:2001-05-01 00:00:00