Abstract:
:Human inositol phosphate multikinase (IPMK) is a multifunctional protein in cellular signal transduction, namely, a multispecific inositol phosphate kinase, phosphatidylinositol 3-kinase, and a scaffold within the mTOR-raptor complex. To fulfill these nuclear and cytoplasmic functions, intracellular targeting of IPMK needs to be regulated. We show here that IPMK, which has been considered to be a preferentially nuclear protein, is a nucleocytoplasmic shuttling protein, whose nuclear export is mediated by classical nuclear export receptor CRM1. We identified a functional nuclear export signal (NES) additionally to its previously described nuclear import signal (NLS). Furthermore, we describe a mechanism by which the activity of the IPMK-NLS is controlled. Protein kinase CK2 binds endogenous IPMK and phosphorylates it at serine 284. Interestingly, this phosphorylation can decrease nuclear localization of IPMK cell type specifically. A controlled nuclear import of IPMK may direct its actions either toward nuclear inositol phosphate (InsPx) metabolism or cytoplasmic actions on InsPx, phosphatidylinositol-4,5-bisphosphate [PtdIns(4,5)P₂], as well as mTOR-raptor.
journal_name
Biol Chemjournal_title
Biological chemistryauthors
Meyer R,Nalaskowski MM,Ehm P,Schröder C,Naj X,Brehm MA,Mayr GWdoi
10.1515/hsz-2011-0209subject
Has Abstractpub_date
2012-03-01 00:00:00pages
149-60issue
3eissn
1431-6730issn
1437-4315pii
/j/bchm.2012.393.issue-3/hsz-2011-0209/hsz-2011-02journal_volume
393pub_type
杂志文章abstract::The ubiquitous calpains, mu- and m-calpain, have been implicated in essential physiological processes and various pathologies. Cell-permeable specific inhibitors are important tools to elucidate the roles of calpains in cultivated cells and animal models. The synthetic N-acetylated 27-mer peptide derived from exon B o...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2003.045
更新日期:2003-03-01 00:00:00
abstract::DEAD-box helicases catalyze RNA duplex unwinding in an ATP-dependent reaction. Members of the DEAD-box helicase family consist of a common helicase core formed by two RecA-like domains. According to the current mechanistic model for DEAD-box mediated RNA unwinding, binding of RNA and ATP triggers a conformational chan...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2014-0277
更新日期:2015-08-01 00:00:00
abstract::Adenoviral DNA was examined within capsids by dimethyl sulfate footprinting. Protein-DNA interactions were visualized through ligation-mediated PCR (LM-PCR). Signals for protein binding were found adjacent to both inverted terminal repeats (ITR). There were no indications of close protein binding at several other loci...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2001.169
更新日期:2001-09-01 00:00:00
abstract::Studies of extracellular matrix (ECM) biology in the nervous system have mainly focused on laminin, fibronectin and tenascin-R, proteins that are present during nervous system development and normal function. However, during disease, fibrin, which physiologically is not present in the nervous tissue, is detected at ne...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2002.004
更新日期:2002-01-01 00:00:00
abstract::Understanding the molecular mechanisms behind extreme temperature stability is of relevance for the protein folding problem and for designing proteins for industrial and medical applications. A powerful approach for understanding the structural basis of thermostability is the comparison of high resolution structures o...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:
更新日期:1997-03-01 00:00:00
abstract::DEAD-box proteins disrupt or remodel RNA and protein/RNA complexes at the expense of ATP. The catalytic core is composed of two flexibly connected RecA-like domains. The N-terminal domain contains most of the motifs involved in nucleotide binding and serves as a minimalistic model for helicase/nucleotide interactions....
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2011.034
更新日期:2011-04-01 00:00:00
abstract::The three isoforms of human Ins(1,4,5)P3 3-kinase (IP3K) show remarkable differences in their intracellular targeting. Whereas predominant targeting to the cytoskeleton and endoplasmic reticulum has been shown for IP3K-A and IP3K-B, rat IP3K-C shuttles actively between the nucleus and cytoplasm. In the present study w...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2006.075
更新日期:2006-05-01 00:00:00
abstract::The vast majority of malignancies detected in renal parenchyma are diagnosed as renal cell carcinoma (RCC), whose subtype discrimination and determination of prognosis may contribute to the selection of the adequate therapy. Recently, a new class of small non-coding RNAs, known as microRNAs, has proven to be among the...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2015-0284
更新日期:2016-06-01 00:00:00
abstract::Abstract Fanconi anemia (FA) is a rare cancer-prone genetic disorder characterized by progressive bone marrow failure, chromosomal instability and redox abnormalities. There is much biochemical and genetic data, which strongly suggest that FA cells experience increased oxidative stress. The present study was designed ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2011.064
更新日期:2011-07-01 00:00:00
abstract::Full-length Hsp70 protein (Hsp70) and the C-terminal domain of Hsp70 (Hsp70C) both stimulate the cytolytic activity of naive natural killer (NK) cells against Hsp70-positive tumor target cells. Here, we describe the characterization of Hsp70-NK cell interaction with binding studies using the human NK cell line YT. Bin...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2003.030
更新日期:2003-02-01 00:00:00
abstract::Upon activation of the B cell antigen receptor (BCR), the spleen tyrosine kinase (Syk) and the Src family kinase Lyn phosphorylate tyrosines of the immunoreceptor tyrosine-based activation motif (ITAM) of Igα and Igβ which further serve as binding sites for the SH2 domains of these kinases. Using a synthetic biology a...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2018-0308
更新日期:2019-03-26 00:00:00
abstract::While the role of C2-ceramide in the induction of programmed cell death (PCD) in animal systems has been well documented, little is known of its role in plant cells. Here we show that C2-ceramide induces PCD in Arabidopsis suspension cultures, which is preceded by the generation of a calcium transient and an increase ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2005.020
更新日期:2005-02-01 00:00:00
abstract::The T-knot scaffold, a disulphide-reinforced structural motif shared by several proteins with very different biological functions, has been defined as 'a stretch of the protein chain which comprises two strands of a beta-sheet and three loops, knotted by two disulphides into the shape of the letter T'. In this communi...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.1999.159
更新日期:1999-10-01 00:00:00
abstract::Haptoglobin (Hpt) is known to capture circulating free hemoglobin (Hb) and bind apolipoprotein (Apo) A-I or E. Here, we report that Hb can be tightly bound by most of Hpt molecules (TB-Hpt, 80%), whereas loosely bound by a minor part of them (LB-Hpt, 20%). LB-Hpt amount was significantly increased (over 60%) in patien...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2011.033
更新日期:2011-04-01 00:00:00
abstract::CYP20A1 is a well-conserved member of the human cytochrome P450 enzyme family for which no endogenous or xenobiotic substrate is known. We have recently shown that this enzyme has moderate activity towards two proluciferin probe substrates. In order to facilitate the search for physiological substrates we have tested ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2019-0307
更新日期:2020-02-25 00:00:00
abstract::DHX36 is a member of the DExD/H box helicase family, which comprises a large number of proteins involved in various cellular functions. Recently, the function of DHX36 in the regulation of G-quadruplexes (G4s) was demonstrated. G4s are alternative nucleic acid structures, which influence many cellular pathways on a tr...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2020-0292
更新日期:2020-10-01 00:00:00
abstract::The three-dimensional structure of pterin-4a-carbinolamine dehydratase (PCD) from Pseudomonas aeruginosa has been solved. Based on this we have investigated the roles of putative active center residues through functional replacement by site-directed mutagenesis. Three histidines, His73, His74 and His91, appear to be i...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/bchm.1998.379.12.1427
更新日期:1998-12-01 00:00:00
abstract::The transcription factor E2F1 plays a decisive role in the G1/S and G2/M checkpoint transitions of proliferating cells. Because cells are arrested at these checkpoints after heat shock it was of interest to test heat shock effects on E2F1 activity. In human A549 cells, heat shock (44 degrees C, 30 min) caused an immed...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2003.017
更新日期:2003-01-01 00:00:00
abstract::The heterodimer consisting of ecdysteroid receptor (EcR) and ultraspiracle (USP), both of which are members of the nuclear receptor superfamily, is considered to be the functional ecdysteroid receptor. Here we analyzed the subcellular distribution of EcR and USP fused to fluorescent proteins. The experiments were carr...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2005.055
更新日期:2005-05-01 00:00:00
abstract::The Sialyl-Tn antigen (Sialyl alpha-Ser/Thr) is expressed as a cancer-associated antigen on the surface of cancer cells. Its presence is associated with a poor prognosis in patients with colorectal and other cancers. We previously reported that Sialyl-Tn expression in LSC human colon cancer cells could be explained by...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2001.029
更新日期:2001-02-01 00:00:00
abstract::Sphingolipids and glycosphingolipids can regulate cell recognition and signalling. Ceramide and sphingosine-1-phosphate are major players in the sphingolipid pathways and are involved in the initiation and regulation of signalling, apoptosis, stress responses and infection. Specific chemically synthesised sphingolipid...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2018-0169
更新日期:2018-09-25 00:00:00
abstract::Cytokinins are essential plant hormones that regulate numerous physiological processes. Recently, a protein was identified in mung bean (Vigna radiata) and characterized as a cytokinin-specific binding protein (VrCSBP). Fluorescence correlation spectroscopy was used to investigate the interaction between VrCSBP and it...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2010.005
更新日期:2010-01-01 00:00:00
abstract::Small RNAs such as microRNAs (miRNAs), short interfering RNAs (siRNAs) or Piwi-interacting RNAs (piRNAs) are important regulators of gene expression in various organisms. Small RNAs bind to a member of the Argonaute protein family and are incorporated into larger structures that mediate diverse gene silencing events. ...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2014-0116
更新日期:2014-06-01 00:00:00
abstract::Matrix metalloproteases from the cell surface cleave an 80 kDa E-cadherin fragment (sE-CAD) that induces invasion of cancer cells into collagen type I and inhibits cellular aggregation. Conditioned media from MDCKts.srcCl2 cells at 40 degrees C and 35 degrees C, PCm.src5 and COLO-16 cells at 37 degrees C contained spo...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2002.016
更新日期:2002-01-01 00:00:00
abstract::Airway smooth muscle (ASM) controls airway narrowing and plays a pivotal role in the pathogenesis of asthma. MicroRNAs are small yet powerful gene tuners that regulate diverse cellular processes. Recent studies have demonstrated the versatile role of microRNAs in regulating multiple ASM phenotypes that are critically ...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2015-0298
更新日期:2016-06-01 00:00:00
abstract::The cloning of a Ca2+(0)-sensing receptor (CaR) from parathyroid and kidney, coupled with the identification of inherited disorders in Ca2+(0)-sensing resulting from inactivating or activating mutations of this CaR, has provided substantial insights into how Ca2+(0) is regulated at a systemic level. The CaR plays a ce...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:
更新日期:1997-08-01 00:00:00
abstract::We have previously shown that compatible organic osmolytes, such as betaine, myo-inositol and taurine, are part of the stress response of normal human keratinocytes (NHKs) to ultraviolet B (UVB) radiation. In this regard, we tested human HaCaT keratinocytes as a surrogate cell line for NHK. HaCaT cells osmo-dependentl...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2007.140
更新日期:2007-12-01 00:00:00
abstract::Mutations of the p53 gene may alter the specific regulatory domains of the protein. We examined the conserved domains III, IV and V by SSCP using PCR primers covering exons 5, 6, 7 and 8 from hairy cell leukemia (HCL), polyps, colorectal and gastric carcinomas. A low rate of p53 mutations was detected in HCL and polyp...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:
更新日期:1998-04-01 00:00:00
abstract::The hypoxia-inducible factor-1 (HIF-1) is a basic-helix-loop-helix (bHLH) heterodimeric transcription factor activated by reductions in oxygen concentration (hypoxia). Activated HIF-1 upregulates expression of genes involved in the adaptation of higher organisms to hypoxic conditions, caused by e.g. high altitude, ane...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:
更新日期:1997-07-01 00:00:00
abstract::Chitinase-like proteins (CLPs) are lectins combining properties of cytokines and growth factors. Human CLPs include YKL-40, YKL-39 and SI-CLP that are secreted by cancer cells, macrophages, neutrophils, synoviocytes, chondrocytes and other cells. The best investigated CLP in cancer is YKL-40. Serum and plasma levels o...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2015-0269
更新日期:2016-03-01 00:00:00