Abstract：
:Mutations of the p53 gene may alter the specific regulatory domains of the protein. We examined the conserved domains III, IV and V by SSCP using PCR primers covering exons 5, 6, 7 and 8 from hairy cell leukemia (HCL), polyps, colorectal and gastric carcinomas. A low rate of p53 mutations was detected in HCL and polyps. These mutations may predict the risk of malignant development. However, multiple mutations were a frequent occurrence in tumors. Sequence analysis of our samples did not demonstrate the high frequency of transition mutations (C-->T) that would be predicted if the major course of p53 mutations is deamination of 5-methylcytosine (5mC). Rather, most mutations were found to be single base insertions or deletions.
journal_name
Biol Chemjournal_title
Biological chemistryauthors
Marcsek ZL,König EAsubject
Has Abstractpub_date
1998-04-01 00:00:00pages
545-7issue
4-5eissn
1431-6730issn
1437-4315journal_volume
379pub_type
杂志文章abstract：:This review deals with current concepts on the regulation and function of phospholipid asymmetry in biological membranes. This ubiquitous phenomenon is characterized by a distinctly different lipid composition between the inner and outer leaflet of the membrane bilayer. Transbilayer asymmetry is controlled by differen...
journal_title：Biological chemistry
pub_type： 杂志文章,评审
doi：
更新日期：1998-08-01 00:00:00
abstract：:γ-Secretase is a membrane-embedded protease complex with presenilin as the catalytic component. Cleavage within the transmembrane domain of the amyloid β-protein precursor (APP) by γ-secretase produces the C-terminus of the amyloid β-peptide (Aβ), a proteolytic product prone to aggregation and strongly linked to Alzhe...
journal_title：Biological chemistry
pub_type： 杂志文章,评审
doi：10.1515/hsz-2012-0140
更新日期：2012-09-01 00:00:00
abstract：:The average amount of cholesterol in the whole animal equals approximately 2100 mg/kg body weight, and 15% and 23% of this sterol in the mouse and human, respectively, is found in the central nervous system. There is no detectable uptake across the blood-brain barrier of cholesterol carried in lipoproteins in the plas...
journal_title：Biological chemistry
pub_type： 杂志文章,评审
doi：10.1515/BC.2009.035
更新日期：2009-04-01 00:00:00
abstract：:As the outermost barrier of the body, the skin is directly and frequently exposed to a prooxidative environment, including solar UVA and UVB radiation, and air pollution. Ozone, a major air pollutant, possesses a strong oxidizing potential and therefore is possibly one of the most reactive chemicals the skin ever meet...
journal_title：Biological chemistry
pub_type： 杂志文章,评审
doi：10.1515/bchm.1997.378.11.1299
更新日期：1997-11-01 00:00:00
abstract：:Sphingolipids and glycosphingolipids can regulate cell recognition and signalling. Ceramide and sphingosine-1-phosphate are major players in the sphingolipid pathways and are involved in the initiation and regulation of signalling, apoptosis, stress responses and infection. Specific chemically synthesised sphingolipid...
journal_title：Biological chemistry
pub_type： 杂志文章,评审
doi：10.1515/hsz-2018-0169
更新日期：2018-09-25 00:00:00
abstract：:Kallikrein-related peptidase 2 (KLK2) degrades insulin-like growth factor (IGF)-binding protein-3 (IGFBP-3) in vitro. IGFBP-3 forms complexes with IGFs, preventing them from binding to their receptors and stimulating cell proliferation and survival. IGF-independent actions have also been described for IGFBP-3. The deg...
journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/BC.2010.039
更新日期：2010-04-01 00:00:00
abstract：:The pancreatic β-cell surface protein Tmem27 is promotes the preservation of functional β-cell mass. It is a selective substrate of the protease Bace2, yet the intramolecular features of Tmem27 that regulate its processing by this sheddase have not been characterized. In particular, the importance of homodimerization,...
journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/hsz-2012-0104
更新日期：2012-05-01 00:00:00
abstract：:We present a rapid and simple method for the sexing of human and other primate DNA. Homologous introns from the X- and Y-linked zinc finger protein genes (ZFX and ZFY) are PCR amplified with one primer pair. An Alu element insertion in the ZFX intron results in a size difference in the amplicons which is resolved by a...
journal_title：Biological chemistry
pub_type： 杂志文章
doi：
更新日期：1998-10-01 00:00:00
abstract：:The primary cilium is a sensory organelle that is vital in regulating several signalling pathways. Unlike most organelles cilia are open to the rest of the cell, not enclosed by membranes. The distinct protein composition is crucial to the function of cilia and many signalling proteins and receptors are specifically c...
journal_title：Biological chemistry
pub_type： 杂志文章,评审
doi：10.1515/hsz-2017-0168
更新日期：2017-12-20 00:00:00
abstract：:Hsp70 chaperone systems play an essential role in the life cycle of many proteins not only in an hostile environment but also under normal growth conditions. In the course of evolution the diversification of functions was accompanied by an amplification of components of the Hsp70 system. Here strategies are reviewed h...
journal_title：Biological chemistry
pub_type： 杂志文章,评审
doi：
更新日期：1998-03-01 00:00:00
abstract：:We developed a novel method for the simultaneous extraction and analysis of total tissue RNA and DNA to quantify the RNA and DNA oxidation products 8-oxo-7,8-dihydroguanosine and 8-oxo-7,8-dihydro-2'-deoxyguanosine using HPLC coupled to electrochemical detection (HPLC-ECD). The protein denaturing agents guanidine thio...
journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/BC.2006.014
更新日期：2006-01-01 00:00:00
abstract：:The mechanism of interaction of the lipopolysaccharide (LPS)-binding protein, LBP, with differently composed symmetric and asymmetric planar lipid bilayers was investigated in electrical measurements (membrane current, potential, capacitance). From a change of the inner membrane potential difference, binding of LBP to...
journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/BC.2001.052
更新日期：2001-03-01 00:00:00
abstract：:The activities of cathepsin B, L, J and H in rat liver were significantly increased by starvation if compared with normal diet rats. Furthermore, the activity of cathepsin L increased with glucagon treatment, and the activities of cathepsin L and H decreased significantly with insulin treatment. The changes in catheps...
journal_title：Biological chemistry
pub_type： 杂志文章
doi：
更新日期：1996-07-01 00:00:00
abstract：:In this work we demonstrate that heat and pressure induce only slightly different energetic changes in the unfolded state of RNase A. Using pressure and temperature as denaturants on a significant number of variants, and by determining the free energy of unfolding at different temperatures, we estimated the stability ...
journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/BC.2006.038
更新日期：2006-03-01 00:00:00
abstract：:The 15 human kallikrein-related peptidases (KLKs) are clinically important biomarkers and therapeutic targets of interest in inflammation, cancer, and neurodegenerative disease. KLKs are secreted as inactive pro-forms (pro-KLKs) that are activated extracellularly by specific proteolytic release of their amino-terminal...
journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/hsz-2012-0249
更新日期：2013-01-01 00:00:00
abstract：:Parvulins belong to the family of peptidyl-prolyl cis/trans isomerases (PPIases) assisting in protein folding and in regulating the function of a broad variety of proteins in all branches of life. The human representatives Pin1 and Par14/17 are directly involved in processes influencing cellular maintenance and cell f...
journal_title：Biological chemistry
pub_type： 杂志文章,评审
doi：10.1515/hsz-2017-0137
更新日期：2018-01-26 00:00:00
abstract：:HB13 human myoblasts express physiological and biochemical markers associated with myoblast differentiation in non-human cell culture model systems. During differentiation, HB13 myoblasts also demonstrate fusion-related increases in cathepsin B activity and protein levels. These increases are associated with an increa...
journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/BC.2002.207
更新日期：2002-12-01 00:00:00
abstract：:The TNF family molecule RANKL and its receptor RANK are key regulators of bone remodeling, lymph node formation, and mammary gland development during pregnancy. RANKL and RANK are also expressed in the central nervous systems (CNS). However, the functional relevance of RANKL/RANK in the brain was entirely unknown. Rec...
journal_title：Biological chemistry
pub_type： 杂志文章,评审
doi：10.1515/BC.2010.149
更新日期：2010-12-01 00:00:00
abstract：:Studies of extracellular matrix (ECM) biology in the nervous system have mainly focused on laminin, fibronectin and tenascin-R, proteins that are present during nervous system development and normal function. However, during disease, fibrin, which physiologically is not present in the nervous tissue, is detected at ne...
journal_title：Biological chemistry
pub_type： 杂志文章,评审
doi：10.1515/BC.2002.004
更新日期：2002-01-01 00:00:00
abstract：:We have previously described Kunitz-type serine proteinase inhibitors purified from Bauhinia seeds. Human plasma kallikrein shows different susceptibility to those inhibitors. In this communication, we describe the interaction of human plasma kallikrein with fluorogenic and non-fluorogenic peptides based on the Bauhin...
journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/BC.2001.016
更新日期：2001-01-01 00:00:00
abstract：:Seven purified proteins may be combined to reconstitute regulated, promoter-dependent RNA polymerase II transcription: five general transcription factors, Mediator, and RNA polymerase II. The entire system has been conserved across species from yeast to humans. The structure of RNA polymerase II, consisting of 10 poly...
journal_title：Biological chemistry
pub_type：
doi：10.1515/BC.2001.140
更新日期：2001-08-01 00:00:00
abstract：:TNF-alpha is a major pro-inflammatory cytokine that regulates further cytokine induction, especially of IL-1 and IL-6, in many human diseases including cancer, inflammation and immune disorders. In a search for new inhibitors of inducible TNF-alpha promoter activity and expression, cultures of the imperfect fungus Tri...
journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/BC.2007.066
更新日期：2007-06-01 00:00:00
abstract：:The gene product of the yeast VPS1 gene is a member of a family of high-molecular-weight GTP-binding proteins that are involved in diverse cellular processes. The Vps1 protein (Vps1p) was shown to perform an essential function in the yeast secretory pathway. Here, we report the isolation and characterization of a muta...
journal_title：Biological chemistry
pub_type： 杂志文章
doi：
更新日期：1997-10-01 00:00:00
abstract：:In mammalian cells, glucosylceramide (GlcCer), the simplest glycosphingolipid, is hydrolyzed by the lysosomal enzyme acid beta-glucosidase (GlcCerase). In the human metabolic disorder Gaucher disease, GlcCerase activity is significantly decreased owing to one of approximately 200 mutations in the GlcCerase gene. The m...
journal_title：Biological chemistry
pub_type： 杂志文章,评审
doi：10.1515/BC.2008.163
更新日期：2008-11-01 00:00:00
abstract：:MHC (major histocompatibility complex) class I molecules bind intracellular virus-derived peptides in the endoplasmic reticulum (ER) and present them at the cell surface to cytotoxic T lymphocytes. Peptide-free class I molecules at the cell surface, however, could lead to aberrant T cell killing. Therefore, cells ensu...
journal_title：Biological chemistry
pub_type： 杂志文章,评审
doi：10.1515/BC.2004.100
更新日期：2004-09-01 00:00:00
abstract：:The reverse action of a trypsin-free elastase isolated from porcine pancreas was studied in frozen aqueous systems. Under frozen state conditions, porcine pancreatic elastase was able to catalyse peptide bond formation more effectively than in solution at room temperature. The acceptance of free amino acids as nucleop...
journal_title：Biological chemistry
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:We studied the mode of regulation of the activity of mature cathepsin B (CB) by L-cysteine and some related thiols. The activity of CB with Z-Arg-Arg-NHMec as substrate was gradually inhibited over a range of increasing concentration of Cys, Cys methyl ester (CysOMe), Cys ethyl ester (CysOEt), N-acetyl-Cys (N-AcCys) a...
journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/BC.1999.069
更新日期：1999-05-01 00:00:00
abstract：:Cellular volume regulation is fundamental for numerous physiological processes. The volume-regulated anion channel, VRAC, plays a crucial role in regulatory volume decrease. This channel, which is ubiquitously expressed in vertebrates, has been vastly characterized by electrophysiological means. It opens upon cell swe...
journal_title：Biological chemistry
pub_type： 杂志文章,评审
doi：10.1515/hsz-2015-0127
更新日期：2015-09-01 00:00:00
abstract：:The enzyme L-asparaginase is a crucial component in the treatment of acute lymphoblastic leukemia (ALL). As all asparaginases in clinical use are derived from microorganisms, immunological reactions are the most important adverse events associated with asparaginase treatment. Two different methods, phage display and t...
journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/BC.2005.063
更新日期：2005-06-01 00:00:00
abstract：:DEAD-box proteins disrupt or remodel RNA and protein/RNA complexes at the expense of ATP. The catalytic core is composed of two flexibly connected RecA-like domains. The N-terminal domain contains most of the motifs involved in nucleotide binding and serves as a minimalistic model for helicase/nucleotide interactions....
journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/BC.2011.034
更新日期：2011-04-01 00:00:00
