Abstract：
:The gene product of the yeast VPS1 gene is a member of a family of high-molecular-weight GTP-binding proteins that are involved in diverse cellular processes. The Vps1 protein (Vps1p) was shown to perform an essential function in the yeast secretory pathway. Here, we report the isolation and characterization of a mutant allele of the VPS1 gene, causing a dominant-negative vacuolar protein sorting (vps) defect, as demonstrated by the mislocalization of the vacuolar hydrolase carboxypeptidase Y (CPY). DNA sequence analysis of the mutant vps1 allele (vps1d-293) revealed a single point mutation, resulting in an amino acid exchange at position 293 from Ala to Asp. The mutation is located downstream of the tripartite GTP-binding motif found in the amino-terminal half of the protein. The observation that expression of wild-type Vps1p partially suppressed the dominant-negative CPY sorting phenotype indicates competition of a non-functional mutant Vps1 protein and a functional wild-type VPS1p for a Vps1p-binding site of an as yet unknown vacuolar protein sorting factor.
journal_name
Biol Chemjournal_title
Biological chemistryauthors
Finken-Eigen M,Müller S,Köhrer Ksubject
Has Abstractpub_date
1997-10-01 00:00:00pages
1187-91issue
10eissn
1431-6730issn
1437-4315journal_volume
378pub_type
杂志文章abstract：:Fibroblast growth factor (FGF) receptor substrate 2 (FRS2) is a membrane-anchored docking protein that has been shown to play an important role in linking FGF, nerve growth factor (NGF) and glial cell-derived neurotrophic factor (GDNF) receptors with the Ras/mitogen-activated protein (MAP) kinase signaling cascade. He...
journal_title：Biological chemistry
pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2001-12-01 00:00:00
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更新日期：2001-01-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2004-11-01 00:00:00
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更新日期：2015-12-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2000-09-01 00:00:00
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更新日期：2007-06-01 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章,评审
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更新日期：2004-06-01 00:00:00
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更新日期：2009-08-01 00:00:00
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journal_title：Biological chemistry
pub_type： 杂志文章,评审
doi：
更新日期：1997-12-01 00:00:00
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journal_title：Biological chemistry
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
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journal_title：Biological chemistry
pub_type： 杂志文章
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更新日期：2015-06-01 00:00:00
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journal_title：Biological chemistry
pub_type： 杂志文章,评审
doi：10.1515/bchm.1998.379.4-5.401
更新日期：1998-04-01 00:00:00
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journal_title：Biological chemistry
pub_type： 杂志文章
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更新日期：1998-04-01 00:00:00
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journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/hsz-2019-0148
更新日期：2019-09-25 00:00:00
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pub_type： 杂志文章
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更新日期：2004-05-01 00:00:00
