Replacement of the interchain disulfide bridge-forming amino acids A7 and B7 by glutamate impairs the structure and activity of insulin.

abstract：:The influence of the hyaluronan-binding protease (PHBSP), a plasma enzyme with FVII- and pro-urokinase-activating potency, on components of the contact phase (kallikrein/kinin) system was investigated. No activation or cleavage of the proenzymes involved in the contact phase system was observed. The pro-cofactor high ...

journal_title：Biological chemistry

authors： Etscheid M,Beer N,Fink E,Seitz R,Johannes D

更新日期：2002-10-01 00:00:00

abstract：:The glutamyl endopeptidase family of enzymes from staphylococci has been shown to be important virulence determinants of pathogenic family members, such as Staphylococcus aureus. Previous studies have identified the N-terminus and residues from positions 185-195 as potentially important regions that determine the acti...

journal_title：Biological chemistry

authors： Ono T,Ohara-Nemoto Y,Shimoyama Y,Okawara H,Kobayakawa T,Baba TT,Kimura S,Nemoto TK

更新日期：2010-10-01 00:00:00

abstract：:Recent evidence suggests that epidermal growth factor receptor (EGFR)-dependent signaling contributes to liver cell proliferation, as well as to apoptotic liver cell death, and represents an important regulator of hepatic regeneration. This article summarizes recent findings on the molecular mechanisms involved in EGF...

journal_title：Biological chemistry

authors： Reinehr R,Häussinger D

更新日期：2009-10-01 00:00:00

abstract：:Protein phosphatase 2A (PP2A) constitutes one of the major families of protein serine/threonine phosphatases found in all eukaryotic cells. PP2A holoenzymes are composed of a catalytic subunit complexed with a structural regulatory subunit of 65 kDa. These core subunits associate with regulatory subunits of various si...

journal_title：Biological chemistry

authors： Götz J,Kues W

更新日期：1999-09-01 00:00:00

abstract：:Bradykinin causes vascular relaxations through release of endothelial relaxing factors including prostacyclin, nitric oxide (NO) and epoxyeicosatrienoic acids (EETs). Bradykinin is metabolized by angiotensin converting enzyme (ACE) and ACE inhibition enhances bradykinin relaxations. Our goal was to characterize the ro...

journal_title：Biological chemistry

authors： Gauthier KM,Cepura CJ,Campbell WB

更新日期：2013-09-01 00:00:00

abstract：:Oncogenic transformation is dependent on activated membrane-associated NADPH oxidase (NOX). However, the resultant extracellular superoxide anions are also driving the NO/peroxynitrite and the HOCl pathway, which eliminates NOX-expressing transformed cells through selective apoptosis induction. Tumor progression is de...

journal_title：Biological chemistry

authors： Böhm B,Heinzelmann S,Motz M,Bauer G

更新日期：2015-12-01 00:00:00

abstract：:DEAD-box helicases catalyze RNA duplex unwinding in an ATP-dependent reaction. Members of the DEAD-box helicase family consist of a common helicase core formed by two RecA-like domains. According to the current mechanistic model for DEAD-box mediated RNA unwinding, binding of RNA and ATP triggers a conformational chan...

journal_title：Biological chemistry

authors： Rudolph MG,Klostermeier D

更新日期：2015-08-01 00:00:00

abstract：:Components of kinin-forming systems operating at inflammatory sites are likely to interact with elastase that is released by recruited neutrophils and may, at least temporarily, constitute the major proteolytic activity present at these sites. The aim of this work was to determine the effect of kininogen degradation b...

journal_title：Biological chemistry

authors： Dulinski R,Suder P,Guevara-Lora I,Rapała-Kozik M,Potempa J,Silberring J,Imamura T,Travis J,Kozik A

更新日期：2003-06-01 00:00:00

abstract：:Cells need to process multifaceted external cues to steer their dynamic behavior. To efficiently perform this task, cells implement several exploratory mechanisms to actively sample their environment. In particular, cells can use exploratory actin-based cell protrusions and contractions to engage and squeeze the envir...

journal_title：Biological chemistry

authors： Nalbant P,Dehmelt L

更新日期：2018-07-26 00:00:00

abstract：:Fluorescence correlation spectroscopy (FCS) was used in monitoring human parvovirus B19 virus-like particle (VLP) antibody complexes from acute phase and past-immunity serum samples. The Oregon Green 488-labeled VLPs gave an average diffusion coefficient of 1.7 x 10(-7) cm2 s(-1) with an apparent hydrodynamic radius o...

journal_title：Biological chemistry

authors： Toivola J,Michel PO,Gilbert L,Lahtinen T,Marjomäki V,Hedman K,Vuento M,Oker-Blom C

更新日期：2004-01-01 00:00:00

abstract：:Bacterial proteases secreted into an infected host may exhibit a wide range of pathogenic potentials. Staphylococci, in particular Staphylococcus aureus, are known to produce several extracellular proteases, including serine-, cysteine- and metalloenzymes. Their insensitivity to most human plasma protease inhibitors a...

journal_title：Biological chemistry

authors： Dubin G

更新日期：2002-07-01 00:00:00

abstract：:The flagellate Euglena gracilis contains as photoreceptor complex a heterotetrameric light-sensitive adenylyl cyclase (AC), consisting of the flavoproteins PACalpha and PACbeta. Previously, we demonstrated the functional expression of PACalpha and PACbeta in oocytes from Xenopus laevis and of PACalpha in different ani...

journal_title：Biological chemistry

authors： Looser J,Schröder-Lang S,Hegemann P,Nagel G

更新日期：2009-11-01 00:00:00

abstract：:The hypoxia-inducible factor-1 (HIF-1) is a basic-helix-loop-helix (bHLH) heterodimeric transcription factor activated by reductions in oxygen concentration (hypoxia). Activated HIF-1 upregulates expression of genes involved in the adaptation of higher organisms to hypoxic conditions, caused by e.g. high altitude, ane...

journal_title：Biological chemistry

authors： Wenger RH,Gassmann M

更新日期：1997-07-01 00:00:00

abstract：:Glycoconjugates have a whole spectrum of biological roles, from those that appear trivial to those that are crucial. Results accumulated in the past years indicate they might also play an important role in the response to stress, a complex physiological response of the human organism to various threats. We have recent...

journal_title：Biological chemistry

authors： Lauc G,Peter-Katalinić J,Dabelić S,Flögel M

更新日期：1999-04-01 00:00:00

abstract：:We present a novel strategy, termed CISTEM, which allows direct in vivo screening of polypeptides displayed on the surface of E. coli cells by a combination of ligand-mediated protection and phage-mediated selection. The effectiveness of this new approach was demonstrated by displaying the T7.tag on the surface of E. ...

journal_title：Biological chemistry

authors： Camaj P,Hirsh AE,Schmidt W,Meinke A,von Gabain A

更新日期：2001-12-01 00:00:00

abstract：:Molecular chaperones of the heat shock protein 70 (Hsp70) family play a crucial role in the presentation of exogenous antigenic peptides by antigen-presenting cells (APCs). In a combined biochemical and immunological approach, we characterize the biochemical interaction of tumor-associated peptides with human Hsp70 an...

journal_title：Biological chemistry

authors： Pandya MJ,Bendz H,Manzenrieder F,Noessner E,Kessler H,Buchner J,Issels RD

更新日期：2009-04-01 00:00:00

abstract：:CYP20A1 is a well-conserved member of the human cytochrome P450 enzyme family for which no endogenous or xenobiotic substrate is known. We have recently shown that this enzyme has moderate activity towards two proluciferin probe substrates. In order to facilitate the search for physiological substrates we have tested ...

journal_title：Biological chemistry

authors： Durairaj P,Fan L,Sharma SS,Jie Z,Bureik M

更新日期：2020-02-25 00:00:00

abstract：:The efficient execution of apoptotic cell death with the clearance of apoptotic debris by phagocytes is a key regulatory mechanism ensuring tissue homeostasis. Failure in this execution program contributes to the pathogenesis of many human diseases. In this review, we describe the current knowledge regarding the inter...

journal_title：Biological chemistry

authors： Weigert A,Jennewein C,Brüne B

更新日期：2009-05-01 00:00:00

abstract：:Plasma kallikrein plays a role in coagulation, fibrinolysis and inflammation. Cathepsins B and L participate in (patho)physiological processes such as peptide antigen processing, tissue remodeling events, protein turnover in cells, hormone processing and tumor invasion. The present work analyzes the processing of prek...

journal_title：Biological chemistry

authors： Barros NM,Puzer L,Tersariol IL,Oliva ML,Sampaio CA,Carmona AK,Motta G

更新日期：2004-11-01 00:00:00

abstract：:PAR and C/EBP family proteins are liver-enriched basic leucine zipper (bZip) transcription factors that bind similar sites on the promoters of albumin and cholesterol 7 alpha hydroxylase genes. However, C/EBP proteins have a more relaxed binding specificity than PAR proteins, in that they recognize many sites within p...

journal_title：Biological chemistry

authors： Falvey E,Marcacci L,Schibler U

更新日期：1996-12-01 00:00:00

abstract：:Induction of apoptosis by selective BH3-mimetics is currently investigated as a novel strategy for cancer treatment. Here, we report that selective BH3-mimetics induce apoptosis in a variety of hematological malignancies. Apoptosis is accompanied by severe mitochondrial toxicities upstream of caspase activation. Speci...

journal_title：Biological chemistry

authors： Henz K,Al-Zebeeby A,Basoglu M,Fulda S,Cohen GM,Varadarajan S,Vogler M

更新日期：2019-01-28 00:00:00

abstract：:Fibroblast growth factor (FGF) receptor substrate 2 (FRS2) is a membrane-anchored docking protein that has been shown to play an important role in linking FGF, nerve growth factor (NGF) and glial cell-derived neurotrophic factor (GDNF) receptors with the Ras/mitogen-activated protein (MAP) kinase signaling cascade. He...

journal_title：Biological chemistry

authors： Wu Y,Chen Z,Ullrich A

更新日期：2003-08-01 00:00:00

abstract：:Invadolysin is a novel metalloprotease conserved amongst metazoans that is essential for life in Drosophila. We previously showed that invadolysin was essential for the cell cycle and cell migration, linking to metabolism through a role in lipid storage and interaction with mitochondrial proteins. In this study we dem...

journal_title：Biological chemistry

authors： Chang CW,Abhinav K,Di Cara F,Panagakou I,Vass S,Heck MM

更新日期：2017-03-01 00:00:00

abstract：:The physiological function of the GPI-anchored ectoenzyme aminopeptidase P (APP) is still elusive. Most researchers suppose that this enzyme inactivates biologically active peptides like bradykinin, neuropeptide tyrosine (NPY) and others (Vanhoof et al., 1995). We demonstrate by immunohistology with a specific antibod...

journal_title：Biological chemistry

authors： Lasch J,Moschner S,Sann H,Zellmer S,Koelsch R

更新日期：1998-06-01 00:00:00

abstract：:We have investigated the methylation status of the repeat unit of the human ribosomal RNA genes in lung cancer. Using a Southern blot analysis approach we have determined that the non-transcribed region of these genes was generally heavily methylated, while the transcribed region was not methylated in either tumor or ...

journal_title：Biological chemistry

authors： Shiraishi M,Sekiguchi A,Chuu YH,Sekiya T

更新日期：1999-01-01 00:00:00

abstract：:Abstract The homeostasis and atheroprotective function of bilirubin could be an appealing model to investigate one of the many physiologic functions of the human aryl hydrocarbon receptor (AhR). Several clinical and epidemiological studies have been carried out on key enzymes generating and eliminating bilirubin (heme...

journal_title：Biological chemistry

authors： Bock KW,Köhle C

更新日期：2010-06-01 00:00:00

abstract：:Gaucher disease (GD) is a lysosomal storage disorder, caused by an impaired function of β-glucocerebrosidase, which results in accumulation of glucocerebroside in cells, and altered membrane ordering. Using electron paramagnetic resonance spin labeling, a statistically significant difference in the order parameter bet...

journal_title：Biological chemistry

authors： Pavićević A,Lakočević M,Popović M,Popović-Bijelić A,Daković M,Mojović M

更新日期：2018-04-25 00:00:00

abstract：:We have previously shown that compatible organic osmolytes, such as betaine, myo-inositol and taurine, are part of the stress response of normal human keratinocytes (NHKs) to ultraviolet B (UVB) radiation. In this regard, we tested human HaCaT keratinocytes as a surrogate cell line for NHK. HaCaT cells osmo-dependentl...

journal_title：Biological chemistry

authors： Warskulat U,Brookmann S,Reinen A,Häussinger D

更新日期：2007-12-01 00:00:00

abstract：:The regulation of eukaryotic gene expression is a complicated process involving the interaction of a large number of transacting factors with specific cis-regulatory elements. DNA methylation plays a role in this scheme by acting in cis to modulate protein-DNA interactions. Several lines of evidence indicate that meth...

journal_title：Biological chemistry

authors： Bergman Y,Mostoslavsky R

更新日期：1998-04-01 00:00:00

abstract：:The three-dimensional structure of pterin-4a-carbinolamine dehydratase (PCD) from Pseudomonas aeruginosa has been solved. Based on this we have investigated the roles of putative active center residues through functional replacement by site-directed mutagenesis. Three histidines, His73, His74 and His91, appear to be i...

journal_title：Biological chemistry

authors： Köster S,Stier G,Kubasch N,Curtius HC,Ghisla S

更新日期：1998-12-01 00:00:00