Abstract:
:A putative operon of the ribosomal proteins S20 and Thx has been determined in a 1.4 kb sequenced region of T. thermophilus genomic DNA. Both genes have a promoter sequence 29 nt upstream of ORF1, possess their own Shine-Dalgarno motifs (GGAG) and are separated by only 9 nucleotides, a feature characteristic of the compact Thermus thermophilus genome. This is a novel arrangement, since Thx is unique to the Thermus bacteria and in all other prokaryotes the S20 gene is monocistronic. Our results, in conjunction with the recent finding that Thx is located on the top of the head of the 30S subunit in a cavity between multiple RNA elements stabilizing them with its positive charge, corroborate the observation that thermophilic ribosomes require constituents with special features for their stabilization at high temperatures.
journal_name
Biol Chemjournal_title
Biological chemistryauthors
Leontiadou F,Triantafillidou D,Choli-Papadopoulou Tdoi
10.1515/BC.2001.126keywords:
subject
Has Abstractpub_date
2001-07-01 00:00:00pages
1001-6issue
7eissn
1431-6730issn
1437-4315journal_volume
382pub_type
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