Association of betaine-homocysteine S-methyltransferase with microtubules.

abstract：:Here, we describe a novel member in the group of membrane-anchored chymotrypsin (S1)-like serine proteases, namely testis serine protease 1 (T-SP1), as it is principally expressed in testis tissue. The human T-SP1 gene encompasses 28.7 kb on the short arm of chromosome 8 and consists of seven exons. Rapid amplificatio...

journal_title：Biological chemistry

authors： Neth P,Profanter B,Geissler C,Nägler DK,Nerlich A,Sommerhoff CP,Jochum M

更新日期：2008-12-01 00:00:00

abstract：:The pancreatic β-cell surface protein Tmem27 is promotes the preservation of functional β-cell mass. It is a selective substrate of the protease Bace2, yet the intramolecular features of Tmem27 that regulate its processing by this sheddase have not been characterized. In particular, the importance of homodimerization,...

journal_title：Biological chemistry

authors： Esterházy D,Akpinar P,Stoffel M

更新日期：2012-05-01 00:00:00

abstract：:The regulation of eukaryotic gene expression is a complicated process involving the interaction of a large number of transacting factors with specific cis-regulatory elements. DNA methylation plays a role in this scheme by acting in cis to modulate protein-DNA interactions. Several lines of evidence indicate that meth...

journal_title：Biological chemistry

authors： Bergman Y,Mostoslavsky R

更新日期：1998-04-01 00:00:00

abstract：:A major challenge in the research on membrane-anchored and integral membrane protein complexes is to obtain these in a functionally active, water-soluble, and monodisperse form. This requires the incorporation of the membrane proteins into a native-like membrane or detergent micelle that mimics the properties of the o...

journal_title：Biological chemistry

authors： Borch J,Hamann T

更新日期：2009-08-01 00:00:00

abstract：:Reactive oxygen and nitrogen species deposited by cold physical plasma are proposed as predominant effectors in the interaction between discharge and biomedical application. Most reactive species found in plasma sources are known in biology for inter- and intracellular communication (redox signaling) and mammalian cel...

journal_title：Biological chemistry

authors： Wende K,von Woedtke T,Weltmann KD,Bekeschus S

更新日期：2018-12-19 00:00:00

abstract：:Knockout mice are the gold standard to probe for the role of a specific protease within the interacting network of proteases, substrates, and inhibitors. This proteolytic network, or protease web, determines cell signaling and organ homeostasis. Therefore, protease deficiency or inhibition is intrinsically tied to alt...

journal_title：Biological chemistry

authors： Krüger A

更新日期：2009-02-01 00:00:00

abstract：:Cellular proteomes are dynamic and adjusted to permanently changing conditions by ATP-fueled proteolytic machineries. Among the five AAA+ proteases in Escherichia coli FtsH is the only essential and membrane-anchored metalloprotease. FtsH is a homohexamer that uses its ATPase domain to unfold and translocate substrate...

journal_title：Biological chemistry

authors： Bittner LM,Arends J,Narberhaus F

更新日期：2017-05-01 00:00:00

abstract：:Human pro-matrix metalloproteinase 3 (proMMP-3) lacking the N-terminal 34 amino acids and the C-terminal hemopexin-like domain was expressed in E. coli and used to investigate the process of proenzyme activation and its interaction with an endogenous inhibitor TIMP-1 during activation. The truncated precursor was puri...

journal_title：Biological chemistry

authors： Suzuki K,Kan CC,Hung W,Gehring MR,Brew K,Nagase H

更新日期：1998-02-01 00:00:00

abstract：:Site-directed spin labeling (SDSL) in combination with electron paramagnetic resonance (EPR) spectroscopy has emerged as an efficient tool to elucidate the structure and the conformational dynamics of proteins under conditions close to the native state. This review article summarizes the basics as well as the recent p...

journal_title：Biological chemistry

authors： Klare JP

更新日期：2013-10-01 00:00:00

abstract：:Studies of extracellular matrix (ECM) biology in the nervous system have mainly focused on laminin, fibronectin and tenascin-R, proteins that are present during nervous system development and normal function. However, during disease, fibrin, which physiologically is not present in the nervous tissue, is detected at ne...

journal_title：Biological chemistry

authors： Akassoglou K,Strickland S

更新日期：2002-01-01 00:00:00

abstract：:This is the first report describing the enhancement of xylanase activity by the neutral amino acid glycine. Xylanase activity is increased seven-fold at alkaline pH in the presence of glycine and its pH optimum is shifted from pH 7 to 8 without using any protein engineering techniques. Analysis of the steady-state kin...

journal_title：Biological chemistry

authors： Vathipadiekal V,Verma A,Rao M

更新日期：2007-01-01 00:00:00

abstract：:G-protein coupled receptor 119 (GPR119) is emerging as a potential target for the treatment of type 2 diabetes with beneficial effects on glucose homeostasis. This study assessed the insulin-secreting properties of various GPR119 agonists and the distribution of GPR119 in pancreatic islets. Endogenous ligands [oleoyle...

journal_title：Biological chemistry

authors： Moran BM,Abdel-Wahab YH,Flatt PR,McKillop AM

更新日期：2014-04-01 00:00:00

abstract：:The adipocytokine adiponectin and its receptor (AdipoR) comprise a new receptor-ligand system that is involved in a variety of clinically important morbidities such as obesity, type 2 diabetes and cardiovascular diseases. Adiponectin exerts a multitude of beneficial and tissue specific effects depending on its unique,...

journal_title：Biological chemistry

authors： Heiker JT,Kosel D,Beck-Sickinger AG

更新日期：2010-09-01 00:00:00

abstract：:The cysteine-type peptidase cathepsin X is highly upregulated in several cancers and presumably promotes tumor invasion through bypassing cellular senescence. Here, we present first evidence that the underlying mechanism may involve the regulation of the insulin-like growth factor (IGF) system, a well-known activator ...

journal_title：Biological chemistry

authors： Kraus S,Fruth M,Bunsen T,Nägler DK

更新日期：2012-12-01 00:00:00

abstract：:Host T cell activation, a key step in obtaining adaptive immunity against pathogens, is initiated by the binding of the T cell receptor to a foreign antigenic peptide presented by the major histocompatibility complex on the surface of an antigen-presenting cell and, consequently, formation of an immunological synapse....

journal_title：Biological chemistry

authors： Beyersdorf N,Müller N

更新日期：2015-06-01 00:00:00

abstract：:The immunoproteasome subunit low molecular weight protein 2 (LMP2) codon 60 polymorphism has been associated with autoimmune diseases. It has also been demonstrated to influence susceptibility to TNF-alpha-induced apoptosis in blood cells and proteasome activity in aged human brain. In the present study, an in silico ...

journal_title：Biological chemistry

authors： Mishto M,Santoro A,Bellavista E,Sessions R,Textoris-Taube K,Dal Piaz F,Carrard G,Forti K,Salvioli S,Friguet B,Kloetzel PM,Rivett AJ,Franceschi C

更新日期：2006-04-01 00:00:00

abstract：:In this work we demonstrate that heat and pressure induce only slightly different energetic changes in the unfolded state of RNase A. Using pressure and temperature as denaturants on a significant number of variants, and by determining the free energy of unfolding at different temperatures, we estimated the stability ...

journal_title：Biological chemistry

authors： Font J,Benito A,Torrent J,Lange R,Ribó M,Vilanova M

更新日期：2006-03-01 00:00:00

abstract：:The kallikrein-related peptidase (KLK) gene family of 15 serine proteases encodes many proteins, including prostate specific antigen (PSA or KLK3), that are well described and/or are potential biomarkers for hormone-related cancers. Variant mRNA transcripts produced by alternative splicing, polyadenylation or AUG site...

journal_title：Biological chemistry

authors： Tan OL,Whitbread AK,Clements JA,Dong Y

更新日期：2006-06-01 00:00:00

abstract：:It is now appreciated that mitochondria form tubular networks that adapt to the requirements of the cell by undergoing changes in their shape through fission and fusion. Proper mitochondrial distribution also appears to be required for ATP delivery and calcium regulation, and, in some cases, for cell development. Whil...

journal_title：Biological chemistry

authors： Frazier AE,Kiu C,Stojanovski D,Hoogenraad NJ,Ryan MT

更新日期：2006-12-01 00:00:00

abstract：:There is increasing evidence that apoptosis and necrosis represent only two of several possible ways for cells to die. These two types of demise can occur simultaneously in tissues or cell cultures exposed to the same stimulus, and often local metabolic conditions and the intensity of the same initial insult decide th...

journal_title：Biological chemistry

authors： Nicotera P,Leist M,Single B,Volbracht C

更新日期：1999-09-01 00:00:00

abstract：:The influence of the cellular milieu, a complex and crowded solvent, is often neglected when biomolecular structure and function are studied in vitro. To mimic the cellular environment, crowding effects are commonly induced in vitro using artificial crowding agents like Ficoll or dextran. However, it is unclear if suc...

journal_title：Biological chemistry

authors： Gnutt D,Ebbinghaus S

更新日期：2016-01-01 00:00:00

abstract：:Five moeA mutants were generated by replacing some conserved amino acids of MoeA by site-directed mutagenesis. The mutants were assayed for the ability to restore in vivo nitrate reductase activity of the moeA mutant Escherichia coli JRG97 and in vitro Neurospora crassa nit-1 nitrate reductase activity. The replacemen...

journal_title：Biological chemistry

authors： Sandu C,Brandsch R

更新日期：2002-02-01 00:00:00

abstract：:Hsp70 chaperone systems play an essential role in the life cycle of many proteins not only in an hostile environment but also under normal growth conditions. In the course of evolution the diversification of functions was accompanied by an amplification of components of the Hsp70 system. Here strategies are reviewed h...

journal_title：Biological chemistry

authors： Mayer MP,Bukau B

更新日期：1998-03-01 00:00:00

abstract：:Plasma prekallikrein (PPK) is synthesised in hepatocytes and secreted into the blood, where it participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. Recently we demonstrated by quantitative RT-PCR that the human PPK gene is transcribed not only in the l...

journal_title：Biological chemistry

authors： Fink E,Bhoola KD,Snyman C,Neth P,Figueroa CD

更新日期：2007-09-01 00:00:00

abstract：:There are enzymatic and non-enzymatic mechanisms that generate NO* from nitrite in blood, stomach, saliva, urine and skin. In blood vessels, nitrite-derived NO* can provide protection via compensatory vasodilation during hypoxia, and in various body fluids it may have antibacterial activity. In the skin, nitrite-deriv...

journal_title：Biological chemistry

authors： Suschek CV,Schewe T,Sies H,Kröncke KD

更新日期：2006-05-01 00:00:00

abstract：:The relationship between the structure and activity of meta- and para-hydroxylated monophenols was studied during their tyrosinase-catalysed hydroxylation and the rate-limiting steps of the reaction mechanism were identified. The para-hydroxylated substrates permit us to study the effect of a substituent (R) in the ca...

journal_title：Biological chemistry

authors： Fenoll LG,Rodríguez-López JN,Varón R,García-Ruiz PA,García-Cánovas F,Tudela J

更新日期：2000-04-01 00:00:00

abstract：:Arginine deiminase (ADI) is a potential antitumor agent for the arginine deprivation treatment of L-arginine auxotrophic tumors. The optimum pH of ADI varies significantly, yet little is known about the origin of this variety. Here, Pseudomonas aeruginosa ADI (PaADI), an enzyme that functions only at acidic pH, was ut...

journal_title：Biological chemistry

authors： Ding H,Liu H,Yin Y,Ding Y,Jia Y,Chen Q,Zou G,Zheng Z

更新日期：2012-09-01 00:00:00

abstract：:The average amount of cholesterol in the whole animal equals approximately 2100 mg/kg body weight, and 15% and 23% of this sterol in the mouse and human, respectively, is found in the central nervous system. There is no detectable uptake across the blood-brain barrier of cholesterol carried in lipoproteins in the plas...

journal_title：Biological chemistry

authors： Dietschy JM

更新日期：2009-04-01 00:00:00

abstract：:The bifunctional protein DCoH (dimerization cofactor of HNF-1) is a transcriptional coactivator of homeodomain proteins and a cytoplasmic enzyme. Here we report on the isolation, nucleotide sequence and genomic structure of the chicken and rat DCoH genes. They have an unusual first exon coding for a single amino acid....

journal_title：Biological chemistry

authors： Bossow S,Riepl S,Igo-Kemenes T

更新日期：1998-11-01 00:00:00

abstract：:The lysosomal cysteine proteinase cathepsin B has been implicated in the progression of various human tumors including ovarian cancer. Included in this study were 63 patients with epithelial ovarian carcinoma. Follow-up information (median follow-up period 7 years) was available for all patients, among whom 42 (66.7%)...

journal_title：Biological chemistry

authors： Scorilas A,Fotiou S,Tsiambas E,Yotis J,Kotsiandri F,Sameni M,Sloane BF,Talieri M

更新日期：2002-07-01 00:00:00