Abstract:
:The bifunctional protein DCoH (dimerization cofactor of HNF-1) is a transcriptional coactivator of homeodomain proteins and a cytoplasmic enzyme. Here we report on the isolation, nucleotide sequence and genomic structure of the chicken and rat DCoH genes. They have an unusual first exon coding for a single amino acid. The promoters are located within CpG-islands and lack TATA and CAAT consensus sequences. Analysis of the number of CpG dinucleotides in the promoter indicates little changes since man and chicken were separated in evolution whereas a 67% reduction was observed in the rat lineage. The DNA sequences were obtained entirely without cloning. For walking into unknown genomic regions we describe a modification of the ligation-mediated polymerase chain reaction (LM-PCR) that can replace conventional screening and cloning methods and greatly expedite the isolation of genomic DNA.
journal_name
Biol Chemjournal_title
Biological chemistryauthors
Bossow S,Riepl S,Igo-Kemenes Tsubject
Has Abstractpub_date
1998-11-01 00:00:00pages
1359-65issue
11eissn
1431-6730issn
1437-4315journal_volume
379pub_type
杂志文章abstract::Bradykinin causes vascular relaxations through release of endothelial relaxing factors including prostacyclin, nitric oxide (NO) and epoxyeicosatrienoic acids (EETs). Bradykinin is metabolized by angiotensin converting enzyme (ACE) and ACE inhibition enhances bradykinin relaxations. Our goal was to characterize the ro...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2012-0348
更新日期:2013-09-01 00:00:00
abstract::Neuritic plaques of Alzheimer patients are composed of multiple protein components. Among them, the amyloid beta-peptides (Abeta) 1-40/42 and further N- and C-terminally modified fragments of Abeta are highly abundant. Most prominent are the isoaspartate (isoAsp)-Abeta peptides and pyroglutamyl (pGlu)-Abeta. While pGl...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2008.125
更新日期:2008-08-01 00:00:00
abstract::A number of unexpected pathophysiological connections linking different neurodegenerative diseases have emerged over the past decade. An example is provided by prion and Alzheimer's diseases. Despite being distinct pathologies, these disorders share several neurotoxic mechanisms, including accumulation of misfolded pr...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2016-0190
更新日期:2016-11-01 00:00:00
abstract::FK506-binding proteins (FKBPs) belong to a distinct class of immunophilins that interact with immunosuppressants. They use their peptidyl-prolyl isomerase (PPIase) activity to catalyze the cis-trans conversion of prolyl bonds in proteins during protein-folding events. FKBPs also act as a unique group of chaperones. Th...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2017-0251
更新日期:2018-04-25 00:00:00
abstract::Site-directed spin labeling (SDSL) in combination with electron paramagnetic resonance (EPR) spectroscopy has emerged as an efficient tool to elucidate the structure and the conformational dynamics of proteins under conditions close to the native state. This review article summarizes the basics as well as the recent p...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2013-0155
更新日期:2013-10-01 00:00:00
abstract::To exploit the B-lymphocyte antigen-CD20 binding capacity of the Ibritumomab tiuxetan (IBTN) monoclonal antibody (mAb) for imaging, the over-expression of B cells in non-Hodgkin's lymphoma (NHL) (a myeloproliferative disorder of the lymphatic system) was investigated. In the current investigation, we present the label...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC-2011-170
更新日期:2012-01-01 00:00:00
abstract::Dog pancreas microsomes represent the key components of the established model system for the analysis of protein transport into the mammalian endoplasmic reticulum. More recently, these microsomes were also employed in cell-free systems which address questions related to protein folding and protein degradation in the ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.1999.149
更新日期:1999-10-01 00:00:00
abstract::The cloning of a Ca2+(0)-sensing receptor (CaR) from parathyroid and kidney, coupled with the identification of inherited disorders in Ca2+(0)-sensing resulting from inactivating or activating mutations of this CaR, has provided substantial insights into how Ca2+(0) is regulated at a systemic level. The CaR plays a ce...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:
更新日期:1997-08-01 00:00:00
abstract::RNAs are key players in life as they connect the genetic code (DNA) with all cellular processes dominated by proteins. They contain a variety of chemical modifications and many RNAs fold into complex structures. Here, we review recent progress in the analysis of RNA modification and structure on the basis of stable is...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2018-0447
更新日期:2019-06-26 00:00:00
abstract::Plastids originated from an endosymbiotic event between an early eukaryotic host cell and an ancestor of today's cyanobacteria. During the events by which the engulfed endosymbiont was transformed into a permanent organelle, many genes were transferred from the plastidal genome to the nucleus of the host cell. Protein...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2000.110
更新日期:2000-09-01 00:00:00
abstract::Mammalian apolipoproteinB (apoB) RNA editing is a site-specific deamination reaction that mediates the C to U conversion responsible for apoB48 production in the mammalian small intestine. This process is not detected in chicken apoB RNA. Mammalian apoB RNA editing is mediated by a multicomponent enzyme complex that i...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/bchm.1998.379.8-9.1075
更新日期:1998-08-01 00:00:00
abstract::This is the first report describing the enhancement of xylanase activity by the neutral amino acid glycine. Xylanase activity is increased seven-fold at alkaline pH in the presence of glycine and its pH optimum is shifted from pH 7 to 8 without using any protein engineering techniques. Analysis of the steady-state kin...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2007.007
更新日期:2007-01-01 00:00:00
abstract::γ-Secretase is a membrane-embedded protease complex with presenilin as the catalytic component. Cleavage within the transmembrane domain of the amyloid β-protein precursor (APP) by γ-secretase produces the C-terminus of the amyloid β-peptide (Aβ), a proteolytic product prone to aggregation and strongly linked to Alzhe...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2012-0140
更新日期:2012-09-01 00:00:00
abstract::Human septins comprise a family of 13 genes that encode for >30 protein isoforms with ubiquitous and tissue-specific expressions. Septins are GTP-binding proteins that assemble into higher-order oligomers and filamentous polymers, which associate with cell membranes and the cytoskeleton. In the last decade, much progr...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2013-0233
更新日期:2014-02-01 00:00:00
abstract::Two current hypotheses of O2 sensing in the carotid body (CB) chemoreceptors suggest participation of oxygen reactive (ROS) species, but they are mechanistically opposed. One postulates that hypoxia decreases ROS levels; the other that hypoxia increases them. Yet, both propose that the ensuing alteration in the cellul...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2004.021
更新日期:2004-03-01 00:00:00
abstract::Interleukin-6 (IL-6) is a 185 amino acid residue helical cytokine with various biological activities (e. g. B cell development, acute phase reaction). We have investigated the role of the 168-185 C-terminal region of IL-6 in the induction of fibrinogen synthesis and expression of junB mRNA using synthetic peptides cor...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2001.079
更新日期:2001-04-01 00:00:00
abstract::Bradykinin (BK) is a vasorelaxant, algesic and inflammatory agent. Angiotensin II (AngII) is known to control vascular tone and promote growth, inflammation and artherogenesis. There is evidence for cross talking between BK and AngII receptors. Therefore, the effect of lack of kinin receptors was assessed in mice with...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2009.081
更新日期:2009-09-01 00:00:00
abstract::Abstract The R- and K-gingipain proteases of Porphyromonas gingivalis are involved in proteolysis of haemoglobin from which the defensive dimeric haem pigment is formed. Whilst oxyhaemoglobin is refractory towards K-gingipain, methaemoglobin is rapidly degraded. Ligation of methaemoglobin with N3-, which effectively b...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2008.140
更新日期:2008-09-01 00:00:00
abstract::Hypochlorous acid (HOCl) is a bactericidal compound formed by activated neutrophils during inflammation. Overproduction of HOCl causes damage to tissues at the site of neutrophil accumulation. The deleterious effects of excessive HOCl formation can be attenuated using antioxidants. Thiols and thioethers are known to b...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2002.073
更新日期:2002-03-01 00:00:00
abstract::Rasal is a modular multi-domain protein of the GTPase-activating protein 1 (GAP1) family; its four known members, GAP1m, Rasal, GAP1IP4BP and Capri, have a Ras GTPase-activating domain (RasGAP). This domain supports the intrinsically slow GTPase activity of Ras by actively participating in the catalytic reaction. In t...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2017-0159
更新日期:2017-12-20 00:00:00
abstract::Synthetic biologists aim at engineering controllable biological parts such as DNA, RNA and proteins in order to steer biological activities using external inputs. Proteins can be controlled in several ways, for instance by regulating the expression of their encoding genes with small molecules or light. However, post-t...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2018-0309
更新日期:2019-03-26 00:00:00
abstract::In this review, we discuss how time-resolved Fourier transform infrared (FTIR) spectroscopy is used to understand how GTP hydrolysis is catalyzed by small GTPases and their cognate GTPase-activating proteins (GAPs). By interaction with small GTPases, GAPs regulate important signal transduction pathways and transport m...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2014-0219
更新日期:2015-02-01 00:00:00
abstract::We present a novel strategy, termed CISTEM, which allows direct in vivo screening of polypeptides displayed on the surface of E. coli cells by a combination of ligand-mediated protection and phage-mediated selection. The effectiveness of this new approach was demonstrated by displaying the T7.tag on the surface of E. ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2001.202
更新日期:2001-12-01 00:00:00
abstract::Trypsin and chymotrypsin have similar tertiary structures, although very different substrate specificities. Trypsin hydrolyzes peptides at Lys/Arg residues while chymotrypsin recognizes large hydrophobic residues. Recent work has shown that trypsin is not converted into a protease with chymotrypsin-like activity when ...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:
更新日期:1996-07-01 00:00:00
abstract::It is not rare that controversial indications about the presence or the expression level of multidrug-resistant (MDR) proteins come out from different laboratories upon examination of identical tumor specimens. Distinct aspects, including the use of weakly discriminating monoclonal antibodies (MAbs) and/or unsuitable ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/bchm.1997.378.6.503
更新日期:1997-06-01 00:00:00
abstract::Annexins are a highly conserved protein family that bind to phospholipids in a calcium (Ca2+) - dependent manner. Studies with purified annexins, as well as overexpression and knockdown approaches identified multiple functions predominantly linked to their dynamic and reversible membrane binding behavior. However, mos...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2016-0168
更新日期:2016-10-01 00:00:00
abstract::Most excitatory synaptic transmissions in the central nervous system are mediated by the neurotransmitter glutamate. Binding of glutamate released from the presynaptic membrane causes glutamate receptors in the postsynaptic membrane to open, which results in a transient depolarization of the postsynaptic membrane. The...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2006.024
更新日期:2006-02-01 00:00:00
abstract::We have previously shown that short double-stranded RNAs of specific sequence induce phosphorylation in the activation loop of splicing variant D of the transforming growth factor beta-activated protein kinase 1 (TAK1-D). Here, we further characterize this novel function of TAK1-D and the mechanisms of this dsRNA-trig...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2009.046
更新日期:2009-05-01 00:00:00
abstract::In bacteria, UGA stop codons can be recoded to direct the incorporation of selenocysteine into proteins on the ribosome. Recoding requires a selenocysteine incorporation sequence (SECIS) downstream of the UGA codon, a specialized translation factor SelB, and the non-canonical Sec-tRNASec, which is formed from Ser-tRNA...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2007.108
更新日期:2007-10-01 00:00:00
abstract::We recently reported that human melanoma cells, but not benign melanocytes, aberrantly express kallikrein-related peptidase 7 (KLK7). Here, we show a KLK7 overexpression-mediated decrease of cell adhesion to extracellular matrix binding proteins, associated with downregulation of α5/β1/αv/β3 integrin expression. We al...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2017-0339
更新日期:2018-09-25 00:00:00