Abstract:
:Abstract The R- and K-gingipain proteases of Porphyromonas gingivalis are involved in proteolysis of haemoglobin from which the defensive dimeric haem pigment is formed. Whilst oxyhaemoglobin is refractory towards K-gingipain, methaemoglobin is rapidly degraded. Ligation of methaemoglobin with N3-, which effectively blocks haem dissociation from the protein, prevented haemoglobin breakdown. Haem-free globin was rapidly degraded by K-gingipain. These data emphasise the need for haemoglobin oxidation which encourages haem dissociation and makes the haem-free globin susceptible to proteolytic attack.
journal_name
Biol Chemjournal_title
Biological chemistryauthors
Smalley JW,Birss AJ,Szmigielski B,Potempa Jdoi
10.1515/BC.2008.140subject
Has Abstractpub_date
2008-09-01 00:00:00pages
1235-8issue
9eissn
1431-6730issn
1437-4315pii
10.1515/BC.2008.140journal_volume
389pub_type
杂志文章abstract::Major advances have been made during the last decade in our understanding of adrenal steroid hormone biosynthesis. Two key players in these pathways are the human mitochondrial cytochrome P450 enzymes CYP11B1 and CYP11B2, which catalyze the final steps in the biosynthesis of cortisol and aldosterone. Using data from m...
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