The pathobiochemistry of nitrogen dioxide.


:Nitrogen dioxide (*NO2) is an oxidizing free radical which can initiate a variety of destructive pathways in living systems, and several diseases are suspected to be connected with both exogenously and endogenously formed *NO2. Peroxynitrite (ONOO-/ONOOH) is believed to be an important endogenous source of *NO2 radicals, but other sources, among them enzymatically ones, have been identified recently. It also became clear during the last few years that in vivo formation of 3-nitrotyrosine strictly depends on the availability of *NO2 radicals. Since nitrogen dioxide is a very toxic compound an arsenal of antioxidants (e.g. vitamin C, glutathione, vitamin E, and beta-carotene) must eliminate this harmful radical in vivo. Here the recently identified superoxide (O2*-)-dependent formation of peroxynitrate (O2NOO-) and the central role of vitamin C are of special importance.


Biol Chem


Biological chemistry


Kirsch M,Korth HG,Sustmann R,de Groot H





Has Abstract


2002-03-01 00:00:00












  • Cellular prion protein acquires resistance to proteolytic degradation following copper ion binding.

    abstract::The conversion of cellular prion protein (PrP(C)) into its pathological isoform (PrP(Sc)) conveys an increase in hydrophobicity and induces a partial resistance to proteinase K (PK). Interestingly, co-incubation with high copper ion concentrations also modifies the solubility of PrP(c) and induces a partial PK resista...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Kuczius T,Buschmann A,Zhang W,Karch H,Becker K,Peters G,Groschup MH

    更新日期:2004-08-01 00:00:00

  • Identification of new probe substrates for human CYP20A1.

    abstract::CYP20A1 is a well-conserved member of the human cytochrome P450 enzyme family for which no endogenous or xenobiotic substrate is known. We have recently shown that this enzyme has moderate activity towards two proluciferin probe substrates. In order to facilitate the search for physiological substrates we have tested ...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Durairaj P,Fan L,Sharma SS,Jie Z,Bureik M

    更新日期:2020-02-25 00:00:00

  • Synthetic peptides and fluorogenic substrates related to the reactive site sequence of Kunitz-type inhibitors isolated from Bauhinia: interaction with human plasma kallikrein.

    abstract::We have previously described Kunitz-type serine proteinase inhibitors purified from Bauhinia seeds. Human plasma kallikrein shows different susceptibility to those inhibitors. In this communication, we describe the interaction of human plasma kallikrein with fluorogenic and non-fluorogenic peptides based on the Bauhin...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Oliva ML,Santomauro-Vaz EM,Andrade SA,Juliano MA,Pott VJ,Sampaio MU,Sampaio CA

    更新日期:2001-01-01 00:00:00

  • The hyaluronan-binding serine protease from human plasma cleaves HMW and LMW kininogen and releases bradykinin.

    abstract::The influence of the hyaluronan-binding protease (PHBSP), a plasma enzyme with FVII- and pro-urokinase-activating potency, on components of the contact phase (kallikrein/kinin) system was investigated. No activation or cleavage of the proenzymes involved in the contact phase system was observed. The pro-cofactor high ...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Etscheid M,Beer N,Fink E,Seitz R,Johannes D

    更新日期:2002-10-01 00:00:00

  • A fluorescence correlation spectroscopy study of ligand interaction with cytokinin-specific binding protein from mung bean.

    abstract::Cytokinins are essential plant hormones that regulate numerous physiological processes. Recently, a protein was identified in mung bean (Vigna radiata) and characterized as a cytokinin-specific binding protein (VrCSBP). Fluorescence correlation spectroscopy was used to investigate the interaction between VrCSBP and it...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Zawadzki P,Slósarek G,Boryski J,Wojtaszek P

    更新日期:2010-01-01 00:00:00

  • Novel approach to quorum quenching: rational design of antibacterials in combination with hexahistidine-tagged organophosphorus hydrolase.

    abstract::N-acyl homoserine lactones (AHLs) are quorum sensing (QS) signal molecules used by most Gram-negative pathogenic bacteria. In this article the lactonase activity of the preparations based on hexahistidine-tagged organophosphorus hydrolase (His6-OPH) towards AHLs was studied. Initially, three of the most interesting β-...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Aslanli A,Lyagin I,Efremenko E

    更新日期:2018-07-26 00:00:00

  • Annexins in plasma membrane repair.

    abstract::Disruption of the plasma membrane poses deadly threat to eukaryotic cells and survival requires a rapid membrane repair system. Recent evidence reveal various plasma membrane repair mechanisms, which are required for cells to cope with membrane lesions including membrane fusion and replacement strategies, remodeling o...

    journal_title:Biological chemistry

    pub_type: 杂志文章,评审


    authors: Boye TL,Nylandsted J

    更新日期:2016-10-01 00:00:00

  • A structural network associated with the kallikrein-kinin and renin-angiotensin systems.

    abstract::The kallikrein-kinin and renin-angiotensin (KKS-RAS) systems represent two highly regulated proteolytic systems that are involved in several physiological and pathological processes. Although their protein-protein interactions can be studied using experimental approaches, it is difficult to differentiate between direc...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Stoka V,Turk V

    更新日期:2010-04-01 00:00:00

  • Cis-regulatory sequences from the first intron of the rat glutamine synthetase gene are involved in hepatocyte specific expression of the enzyme.

    abstract::In order to identify regulatory elements involved in the hepatocyte specific expression of the enzyme glutamine synthetase [GS (E.C.] we analyzed the first intron of the rat GS gene. A sequence analysis detected clusters of potential transcription factor binding sites in regions that are hypersensitive for DN...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Gaunitz F,Gaunitz C,Papke M,Gebhardt R

    更新日期:1997-01-01 00:00:00

  • The production and characterization of artificial heterodimers of the restriction endonuclease EcoRV.

    abstract::A novel approach to studying the inter- and intrasubunit communication required for the activity of homodimeric proteins is described. It was developed for the restriction endonuclease EcoRV, but should also be useful for other homodimeric enzymes. Two ecorV genes encoding different EcoRV mutants are coexpressed in th...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Wende W,Stahl F,Pingoud A

    更新日期:1996-10-01 00:00:00

  • A new immunohistochemical methodology for the specific detection of MDR1-P-glycoprotein in human tissues based on phage-displayed peptides mimicking the MM4.17 epitope.

    abstract::It is not rare that controversial indications about the presence or the expression level of multidrug-resistant (MDR) proteins come out from different laboratories upon examination of identical tumor specimens. Distinct aspects, including the use of weakly discriminating monoclonal antibodies (MAbs) and/or unsuitable ...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Poloni F,Castagna M,Felici F,Cianfriglia M

    更新日期:1997-06-01 00:00:00

  • Extracellular localization of catalase is associated with the transformed state of malignant cells.

    abstract::Oncogenic transformation is dependent on activated membrane-associated NADPH oxidase (NOX). However, the resultant extracellular superoxide anions are also driving the NO/peroxynitrite and the HOCl pathway, which eliminates NOX-expressing transformed cells through selective apoptosis induction. Tumor progression is de...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Böhm B,Heinzelmann S,Motz M,Bauer G

    更新日期:2015-12-01 00:00:00

  • Changes of the peripheral blood mononuclear cells membrane fluidity from type 1 Gaucher disease patients: an electron paramagnetic resonance study.

    abstract::Gaucher disease (GD) is a lysosomal storage disorder, caused by an impaired function of β-glucocerebrosidase, which results in accumulation of glucocerebroside in cells, and altered membrane ordering. Using electron paramagnetic resonance spin labeling, a statistically significant difference in the order parameter bet...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Pavićević A,Lakočević M,Popović M,Popović-Bijelić A,Daković M,Mojović M

    更新日期:2018-04-25 00:00:00

  • Inhibition of inducible tumor necrosis factor-alpha expression by the fungal epipolythiodiketopiperazine gliovirin.

    abstract::TNF-alpha is a major pro-inflammatory cytokine that regulates further cytokine induction, especially of IL-1 and IL-6, in many human diseases including cancer, inflammation and immune disorders. In a search for new inhibitors of inducible TNF-alpha promoter activity and expression, cultures of the imperfect fungus Tri...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Rether J,Serwe A,Anke T,Erkel G

    更新日期:2007-06-01 00:00:00

  • Efficient production of native actin upon translation in a bacterial lysate supplemented with the eukaryotic chaperonin TRiC.

    abstract::Recombinant expression of actin in bacteria results in non-native species that aggregate into inclusion bodies. Actin is a folding substrate of TRiC, the chaperonin of the eukaryotic cytosol. By employing bacterial in vitro translation lysates supplemented with purified chaperones, we have found that TRiC is the only ...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Stemp MJ,Guha S,Hartl FU,Barral JM

    更新日期:2005-08-01 00:00:00

  • Replacement of the interchain disulfide bridge-forming amino acids A7 and B7 by glutamate impairs the structure and activity of insulin.

    abstract::Insulin contains three disulfide bonds, one intrachain bond, A6-A11, and two interchain bonds, A7-B7 and A20-B19. Site-directed mutagenesis results (the two cysteine residues of disulfide A7-B7 were replaced by serine) showed that disulfide A7-B7 is crucial to both the structure and activity of insulin. However, chemi...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Guo ZY,Jia XY,Feng YM

    更新日期:2004-12-01 00:00:00

  • The miRNA-kallikrein axis of interaction: a new dimension in the pathogenesis of prostate cancer.

    abstract::Kallikrein-related peptidases (KLKs) are a family of serine proteases that were shown to be useful cancer biomarkers. KLKs have been shown to be dysregulated in prostate cancer (PCa). microRNAs (miRNAs) are short RNA nucleotides that negatively regulate gene expression and have been reportedly dysregulated in PCa. We ...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: White NM,Youssef YM,Fendler A,Stephan C,Jung K,Yousef GM

    更新日期:2012-04-01 00:00:00

  • Mitochondrial morphology and distribution in mammalian cells.

    abstract::It is now appreciated that mitochondria form tubular networks that adapt to the requirements of the cell by undergoing changes in their shape through fission and fusion. Proper mitochondrial distribution also appears to be required for ATP delivery and calcium regulation, and, in some cases, for cell development. Whil...

    journal_title:Biological chemistry

    pub_type: 杂志文章,评审


    authors: Frazier AE,Kiu C,Stojanovski D,Hoogenraad NJ,Ryan MT

    更新日期:2006-12-01 00:00:00

  • The human steroid hydroxylases CYP1B1 and CYP11B2.

    abstract::Major advances have been made during the last decade in our understanding of adrenal steroid hormone biosynthesis. Two key players in these pathways are the human mitochondrial cytochrome P450 enzymes CYP11B1 and CYP11B2, which catalyze the final steps in the biosynthesis of cortisol and aldosterone. Using data from m...

    journal_title:Biological chemistry

    pub_type: 杂志文章,评审


    authors: Bureik M,Lisurek M,Bernhardt R

    更新日期:2002-10-01 00:00:00

  • Purification and cloning of type A/B hnRNP proteins involved in transcriptional activation from the Rat spi 2 gene GAGA box.

    abstract::The GAGA box of the rat serine protease inhibitor 2 (spi 2) genes not only acts as a basal promoter element, but also mediates transcriptional activation by growth hormone and interleukin-6. The GAGA box is separated from the TATA box by only 12 bp, and this close association is required for efficient transcription. H...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Leverrier S,Cinato E,Paul C,Derancourt J,Bemark M,Leanderson T,Legraverend C

    更新日期:2000-11-01 00:00:00

  • Specific inhibition of interleukin-13 activity by a recombinant human single-chain immunoglobulin domain directed against the IL-13 receptor alpha1 chain.

    abstract::Interleukin-13 (IL-13) is a T-cell-derived pleiotropic cytokine of particular medical importance because of its critical role in the development of allergic asthma. The effects of IL-13 on its target cells are mediated through a dimeric transmembrane receptor (IL-13R), which shares the IL-4Ralpha subunit with the IL-4...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Knackmuss S,Krause S,Engel K,Reusch U,Virchow JC,Mueller T,Kraich M,Little M,Luttmann W,Friedrich K

    更新日期:2007-03-01 00:00:00

  • Cellular expression of plasma prekallikrein in human tissues.

    abstract::Plasma prekallikrein (PPK) is synthesised in hepatocytes and secreted into the blood, where it participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. Recently we demonstrated by quantitative RT-PCR that the human PPK gene is transcribed not only in the l...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Fink E,Bhoola KD,Snyman C,Neth P,Figueroa CD

    更新日期:2007-09-01 00:00:00

  • Eremophilane-type sesquiterpenes from fungi and their medicinal potential.

    abstract::Eremophilanes are sesquiterpenes with a rearranged carbon skeleton formed both by plants and fungi, however, almost no plant eremophilanes are found in fungi. These eremophilanes possess mainly phytotoxic, antimicrobial, anticancer and immunomodulatory properties and in this review fungal eremophilanes with bioactivit...

    journal_title:Biological chemistry

    pub_type: 杂志文章,评审


    authors: Yuyama KT,Fortkamp D,Abraham WR

    更新日期:2017-12-20 00:00:00

  • Mapping of B-cell epitopes in E. coli asparaginase II, an enzyme used in leukemia treatment.

    abstract::The enzyme L-asparaginase is a crucial component in the treatment of acute lymphoblastic leukemia (ALL). As all asparaginases in clinical use are derived from microorganisms, immunological reactions are the most important adverse events associated with asparaginase treatment. Two different methods, phage display and t...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Werner A,Röhm KH,Müller HJ

    更新日期:2005-06-01 00:00:00

  • Click reactions with functional sphingolipids.

    abstract::Sphingolipids and glycosphingolipids can regulate cell recognition and signalling. Ceramide and sphingosine-1-phosphate are major players in the sphingolipid pathways and are involved in the initiation and regulation of signalling, apoptosis, stress responses and infection. Specific chemically synthesised sphingolipid...

    journal_title:Biological chemistry

    pub_type: 杂志文章,评审


    authors: Fink J,Seibel J

    更新日期:2018-09-25 00:00:00

  • Topotecan and methotrexate alter expression of the apoptosis-related genes BCL2, FAS and BCL2L12 in leukemic HL-60 cells.

    abstract::The BCL2 family of genes (B-cell CLL/lymphoma 2; Bcl-2) plays a pivotal role in the highly regulated process of apoptosis. We have recently cloned a newly identified member of this family, BCL2L12, which was found to be differentially expressed in many tumors. It is known that topotecan and methotrexate act through in...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Floros KV,Talieri M,Scorilas A

    更新日期:2006-12-01 00:00:00

  • Inhibition of cathepsin L-like proteases by cathepsin V propeptide.

    abstract::The N-terminal propeptide domains of several cathepsin L-like cysteine proteases have been shown to possess potent inhibitory activity. Here we report the first kinetic characterisation of the inhibition properties of the cathepsin V propeptide (CatV PP). Using a facile recombinant approach we demonstrate expression, ...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Burden RE,Snoddy P,Jefferies CA,Walker B,Scott CJ

    更新日期:2007-05-01 00:00:00

  • Impaired synthesis of heme oxygenase-1 in Fanconi anemia cells can be rescued by transfection of Fanconi wild-type cDNA.

    abstract::Fanconi anemia is a fatal, hereditary chromosome instability syndrome of early childhood with progressive pancytopenia and cancer-proneness. Hypersensitivity to alkylating agents points to DNA repair inefficiency. Excess reactive oxygen intermediates and hypersensitivity to oxygen, all features of Fanconi anemia cells...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Kontou M,Hirsch-Kauffmann M,Schweiger M

    更新日期:2008-10-01 00:00:00

  • The Btk inhibitor LFM-A13 is a potent inhibitor of Jak2 kinase activity.

    abstract::LFM-A13, or alpha-cyano-beta-hydroxy-beta-methyl-N-(2,5-dibromophenyl)propenamide, was shown to inhibit Bruton's tyrosine kinase (Btk). Here we show that LFM-A13 efficiently inhibits erythropoietin (Epo)-induced phosphorylation of the erythropoietin receptor, Janus kinase 2 (Jak2) and downstream signalling molecules. ...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: van den Akker E,van Dijk TB,Schmidt U,Felida L,Beug H,Löwenberg B,von Lindern M

    更新日期:2004-05-01 00:00:00

  • Emerging mechanisms of drug-induced phospholipidosis.

    abstract::Drug-induced phospholipidosis is a lysosomal storage disorder characterized by excessive accumulation of phospholipids. Its cellular mechanism is still not well understood, but it is known that cationic amphiphilic drugs can induce it. These drugs have a hydrophilic amine head group that can be protonated in the endol...

    journal_title:Biological chemistry

    pub_type: 杂志文章,评审


    authors: Breiden B,Sandhoff K

    更新日期:2019-12-18 00:00:00