Mannosidase action, independent of glucose trimming, is essential for proteasome-mediated degradation of unassembled glycosylated Ig light chains.

abstract：:Neuritic plaques of Alzheimer patients are composed of multiple protein components. Among them, the amyloid beta-peptides (Abeta) 1-40/42 and further N- and C-terminally modified fragments of Abeta are highly abundant. Most prominent are the isoaspartate (isoAsp)-Abeta peptides and pyroglutamyl (pGlu)-Abeta. While pGl...

journal_title：Biological chemistry

authors： Böhme L,Hoffmann T,Manhart S,Wolf R,Demuth HU

更新日期：2008-08-01 00:00:00

abstract：:92 kDa and 72 kDa gelatinases, two neutral proteinases exhibiting elastinolytic activity and secreted as zymogens by aortic smooth muscle cells, were shown to bind to insoluble elastin. The active form of each enzyme interacted with substrate more avidly than latent form. Once bound to insoluble elastin, 92 kDa progel...

journal_title：Biological chemistry

authors： Emonard H,Hornebeck W

更新日期：1997-03-01 00:00:00

abstract：:Synthetic biologists aim at engineering controllable biological parts such as DNA, RNA and proteins in order to steer biological activities using external inputs. Proteins can be controlled in several ways, for instance by regulating the expression of their encoding genes with small molecules or light. However, post-t...

journal_title：Biological chemistry

authors： Di Ventura B,Mootz HD

更新日期：2019-03-26 00:00:00

abstract：:Metachromatic leukodystrophy is a lysosomal disorder caused by the deficiency of arylsulfatase A (ASA). This leads to the storage of the sphingolipid 3-O-sulfogalactosylceramide (sulfatide) in various cell types, such as renal tubular cells. Examination of mannose 6-phosphate receptor (MPR300)-dependent endocytosis re...

journal_title：Biological chemistry

authors： Klein D,Yaghootfam A,Matzner U,Koch B,Braulke T,Gieselmann V

更新日期：2009-01-01 00:00:00

abstract：:The regulation of eukaryotic gene expression is a complicated process involving the interaction of a large number of transacting factors with specific cis-regulatory elements. DNA methylation plays a role in this scheme by acting in cis to modulate protein-DNA interactions. Several lines of evidence indicate that meth...

journal_title：Biological chemistry

authors： Bergman Y,Mostoslavsky R

更新日期：1998-04-01 00:00:00

abstract：:The GAGA box of the rat serine protease inhibitor 2 (spi 2) genes not only acts as a basal promoter element, but also mediates transcriptional activation by growth hormone and interleukin-6. The GAGA box is separated from the TATA box by only 12 bp, and this close association is required for efficient transcription. H...

journal_title：Biological chemistry

authors： Leverrier S,Cinato E,Paul C,Derancourt J,Bemark M,Leanderson T,Legraverend C

更新日期：2000-11-01 00:00:00

abstract：:It is not rare that controversial indications about the presence or the expression level of multidrug-resistant (MDR) proteins come out from different laboratories upon examination of identical tumor specimens. Distinct aspects, including the use of weakly discriminating monoclonal antibodies (MAbs) and/or unsuitable ...

journal_title：Biological chemistry

authors： Poloni F,Castagna M,Felici F,Cianfriglia M

更新日期：1997-06-01 00:00:00

abstract：:Nitrosative stress results from an increase in reactive nitrogen species (RNS) within the cell. Though the RNS - nitric oxide (·NO) and peroxynitrite (ONOO-) - play pivotal physiological roles, at elevated concentrations, these moieties can be poisonous to both prokaryotic and eukaryotic cells alike due to their capac...

journal_title：Biological chemistry

authors： Tharmalingam S,Alhasawi A,Appanna VP,Lemire J,Appanna VD

更新日期：2017-10-26 00:00:00

abstract：:We recently reported that human melanoma cells, but not benign melanocytes, aberrantly express kallikrein-related peptidase 7 (KLK7). Here, we show a KLK7 overexpression-mediated decrease of cell adhesion to extracellular matrix binding proteins, associated with downregulation of α5/β1/αv/β3 integrin expression. We al...

journal_title：Biological chemistry

authors： Haddada M,Draoui H,Deschamps L,Walker F,Delaunay T,Brattsand M,Magdolen V,Darmoul D

更新日期：2018-09-25 00:00:00

abstract：:Fanconi anemia is a fatal, hereditary chromosome instability syndrome of early childhood with progressive pancytopenia and cancer-proneness. Hypersensitivity to alkylating agents points to DNA repair inefficiency. Excess reactive oxygen intermediates and hypersensitivity to oxygen, all features of Fanconi anemia cells...

journal_title：Biological chemistry

authors： Kontou M,Hirsch-Kauffmann M,Schweiger M

更新日期：2008-10-01 00:00:00

abstract：:The signal recognition particle (SRP), a ubiquitous cytoplasmic ribonucleoprotein particle, plays an essential role in promoting co-translational translocation of proteins into the endoplasmic reticulum. Here, we summarise recent progress made in the understanding of two essential SRP functions: the signal recognition...

journal_title：Biological chemistry

authors： Bui N,Strub K

更新日期：1999-02-01 00:00:00

abstract：:Site-directed spin labeling (SDSL) in combination with electron paramagnetic resonance (EPR) spectroscopy has emerged as an efficient tool to elucidate the structure and the conformational dynamics of proteins under conditions close to the native state. This review article summarizes the basics as well as the recent p...

journal_title：Biological chemistry

authors： Klare JP

更新日期：2013-10-01 00:00:00

abstract：:Five moeA mutants were generated by replacing some conserved amino acids of MoeA by site-directed mutagenesis. The mutants were assayed for the ability to restore in vivo nitrate reductase activity of the moeA mutant Escherichia coli JRG97 and in vitro Neurospora crassa nit-1 nitrate reductase activity. The replacemen...

journal_title：Biological chemistry

authors： Sandu C,Brandsch R

更新日期：2002-02-01 00:00:00

abstract：:Vaccination with either exogenous hepatitis B surface antigen (HBsAg) lipoprotein particles without adjuvants, or plasmid DNA encoding secreted small HBsAg stimulate long-lasting, potent antibody responses in H-2d (BALB/c) and C57Bl/6 (H-2b) mice. Vaccination with exogenous HBsAg primes MHC-I restricted cytotoxic T ly...

journal_title：Biological chemistry

authors： Schirmbeck R,Reimann J

更新日期：1999-03-01 00:00:00

abstract：:RNAs are key players in life as they connect the genetic code (DNA) with all cellular processes dominated by proteins. They contain a variety of chemical modifications and many RNAs fold into complex structures. Here, we review recent progress in the analysis of RNA modification and structure on the basis of stable is...

journal_title：Biological chemistry

authors： Asadi-Atoi P,Barraud P,Tisne C,Kellner S

更新日期：2019-06-26 00:00:00

abstract：:Protein disulfide isomerase (PDI) is a protein of the endoplasmic reticulum (ER) that is essential for the unscrambling of nonnative disulfide bonds. Here, we have determined the importance of PDI to both spore germination and vegetative cell division. To vary the concentration of PDI in the ER, we used plasmids that ...

journal_title：Biological chemistry

authors： Laboissière MC,Sturley SL,Raines RT

更新日期：1997-05-01 00:00:00

abstract：:Mitochondria contain elaborate machineries for the import of precursor proteins from the cytosol. The translocase of the outer mitochondrial membrane (TOM) performs the initial import of precursor proteins and transfers the precursors to downstream translocases, including the presequence translocase and the carrier tr...

journal_title：Biological chemistry

authors： Straub SP,Stiller SB,Wiedemann N,Pfanner N

更新日期：2016-11-01 00:00:00

abstract：:During the last years, the use of therapeutic glycoproteins has increased strikingly. Glycosylation of recombinant glycoproteins is of major importance in biotechnology, as the glycan composition of recombinant glycoproteins impacts their pharmacological properties. The terminal position of N-linked complex glycans in...

journal_title：Biological chemistry

authors： Werner A,Horstkorte R,Glanz D,Biskup K,Blanchard V,Berger M,Bork K

更新日期：2012-08-01 00:00:00

abstract：:Sphingolipids and glycosphingolipids can regulate cell recognition and signalling. Ceramide and sphingosine-1-phosphate are major players in the sphingolipid pathways and are involved in the initiation and regulation of signalling, apoptosis, stress responses and infection. Specific chemically synthesised sphingolipid...

journal_title：Biological chemistry

authors： Fink J,Seibel J

更新日期：2018-09-25 00:00:00

abstract：:Tissue kallikrein KLK1 and the kallikrein-related peptidases KLK2-15 are a subfamily of serine proteases that have defined or proposed roles in a range of central nervous system (CNS) and non-CNS pathologies. To further understand their potential activity in multiple sclerosis (MS), serum levels of KLK1, 6, 7, 8 and 1...

journal_title：Biological chemistry

authors： Scarisbrick IA,Linbo R,Vandell AG,Keegan M,Blaber SI,Blaber M,Sneve D,Lucchinetti CF,Rodriguez M,Diamandis EP

更新日期：2008-06-01 00:00:00

abstract：:Knockout mice are the gold standard to probe for the role of a specific protease within the interacting network of proteases, substrates, and inhibitors. This proteolytic network, or protease web, determines cell signaling and organ homeostasis. Therefore, protease deficiency or inhibition is intrinsically tied to alt...

journal_title：Biological chemistry

authors： Krüger A

更新日期：2009-02-01 00:00:00

abstract：:As an important protein, UL31 has been demonstrated to play multiple roles in herpes simplex virus 1 (HSV-1) replication. Previous studies showed that UL31 predominantly locates in the nucleus in chemical fixed cells and live cells, however, the determining mechanisms for its nuclear translocation is not clear. In the...

journal_title：Biological chemistry

authors： Cai M,Si J,Li X,Zeng Z,Li M

更新日期：2016-06-01 00:00:00

abstract：:We studied the mode of regulation of the activity of mature cathepsin B (CB) by L-cysteine and some related thiols. The activity of CB with Z-Arg-Arg-NHMec as substrate was gradually inhibited over a range of increasing concentration of Cys, Cys methyl ester (CysOMe), Cys ethyl ester (CysOEt), N-acetyl-Cys (N-AcCys) a...

journal_title：Biological chemistry

authors： Krepela E,Procházka J,Kárová B

更新日期：1999-05-01 00:00:00

abstract：:We present a rapid and simple method for the sexing of human and other primate DNA. Homologous introns from the X- and Y-linked zinc finger protein genes (ZFX and ZFY) are PCR amplified with one primer pair. An Alu element insertion in the ZFX intron results in a size difference in the amplicons which is resolved by a...

journal_title：Biological chemistry

authors： Wilson JF,Erlandsson R

更新日期：1998-10-01 00:00:00

abstract：:Calpain is a calcium-activated non lysosomal neutral thiol protease (EC 3.4.22.17) present in a wide variety of eukaryotic cells. Calpain is usually present as an inactive form and is activated by calcium ions and phospholipids. The ability of calpain to alter, by limited proteolysis, the activity or function of numer...

journal_title：Biological chemistry

authors： Yajima Y,Kawashima S

更新日期：2002-05-01 00:00:00

abstract：:A FAD and [4Fe-4S]cluster-containing enzyme from Clostridium aminobutyricum catalyses the reversible dehydration of 4-hydroxybutyryl-CoA to crotonyl-CoA which involves the cleavage of an unactivated C-H bond at the beta-carbon. Transient oxidation of the substrate to an enoxy radical by FAD might facilitate the remova...

journal_title：Biological chemistry

authors： Cinkaya I,Buckel W,Medina M,Gomez-Moreno C,Cammack R

更新日期：1997-08-01 00:00:00

abstract：:Abstract The R- and K-gingipain proteases of Porphyromonas gingivalis are involved in proteolysis of haemoglobin from which the defensive dimeric haem pigment is formed. Whilst oxyhaemoglobin is refractory towards K-gingipain, methaemoglobin is rapidly degraded. Ligation of methaemoglobin with N3-, which effectively b...

journal_title：Biological chemistry

authors： Smalley JW,Birss AJ,Szmigielski B,Potempa J

更新日期：2008-09-01 00:00:00

abstract：:Meprins are zinc-endopeptidases of the astacin family, which are expressed as membrane-bound or secreted forms in renal and intestinal brush-border membranes of mouse, rat and man. There are two types of meprin subunits, alpha and beta, which form disulfide-bonded homo- and heterodimers; further oligomerization is med...

journal_title：Biological chemistry

authors： Becker C,Kruse MN,Slotty KA,Köhler D,Harris JR,Rösmann S,Sterchi EE,Stöcker W

更新日期：2003-05-01 00:00:00

abstract：:The purpose of this work was to study the regulative role of the glutamate receptor found earlier in the brain mitochondria. In the present work a glutamate-dependent signaling system with similar features was detected in mitochondria of the heart. The glutamate-dependent signaling system in the heart mitochondria was...

journal_title：Biological chemistry

authors： Selin AA,Lobysheva NV,Nesterov SV,Skorobogatova YA,Byvshev IM,Pavlik LL,Mikheeva IB,Moshkov DA,Yaguzhinsky LS,Nartsissov YR

更新日期：2016-05-01 00:00:00

abstract：:Studies of extracellular matrix (ECM) biology in the nervous system have mainly focused on laminin, fibronectin and tenascin-R, proteins that are present during nervous system development and normal function. However, during disease, fibrin, which physiologically is not present in the nervous tissue, is detected at ne...

journal_title：Biological chemistry

authors： Akassoglou K,Strickland S

更新日期：2002-01-01 00:00:00