Abstract:
:Meprins are zinc-endopeptidases of the astacin family, which are expressed as membrane-bound or secreted forms in renal and intestinal brush-border membranes of mouse, rat and man. There are two types of meprin subunits, alpha and beta, which form disulfide-bonded homo- and heterodimers; further oligomerization is mediated by non-covalent interactions. Both subunits are translated as proenzymes that have to be activated by removal of an N-terminal propeptide. In the gut, the most probable activator is trypsin. In addition, plasmin has been shown to activate the human alpha subunit in colorectal cancer tissue. In the present study we have overexpressed the human meprin alpha subunit and a His-tagged soluble tail-switch-mutant of meprin beta in Baculovirus-infected insect cells. The recombinant homo-oligomeric proteins were purified by gel filtration and affinity chromatography with yields of up to 10 mg/l cell culture medium and analyzed with regard to their activation mechanism. While both alpha and beta homo-oligomers are activated by trypsin, only meprin alpha homo-oligomers are processed to their mature form by plasmin. These results indicate a different accessibility of the propeptide in meprin homo-oligomers and suggest an explanation for the appearance of meprin hetero-oligomers consisting of active alpha, but latent beta subunits.
journal_name
Biol Chemjournal_title
Biological chemistryauthors
Becker C,Kruse MN,Slotty KA,Köhler D,Harris JR,Rösmann S,Sterchi EE,Stöcker Wdoi
10.1515/BC.2003.092keywords:
subject
Has Abstractpub_date
2003-05-01 00:00:00pages
825-31issue
5eissn
1431-6730issn
1437-4315journal_volume
384pub_type
杂志文章abstract::Stromal cell-derived factor-1alpha (SDF-1alpha) is a strong migratory stimulant for hematopoietic stem and progenitor cells (HSPCs). The hematopoietic cytokines thrombopoietin (TPO), Flt3-ligand (FL), stem cell factor (SCF) and interleukin 11 (IL-11) are able to stimulate amplification of primitive murine hematopoieti...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2008.099
更新日期:2008-07-01 00:00:00
abstract::A putative operon of the ribosomal proteins S20 and Thx has been determined in a 1.4 kb sequenced region of T. thermophilus genomic DNA. Both genes have a promoter sequence 29 nt upstream of ORF1, possess their own Shine-Dalgarno motifs (GGAG) and are separated by only 9 nucleotides, a feature characteristic of the co...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2001.126
更新日期:2001-07-01 00:00:00
abstract::A high-resolution crystallographic structure determination of a protein-ligand complex is generally accepted as the 'gold standard' for structure-based drug design, yet the relationship between structure and affinity is neither obvious nor straightforward. Here we analyze the interactions of a series of serine protein...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2014-0158
更新日期:2014-07-01 00:00:00
abstract::Arginine deiminase (ADI) is a potential antitumor agent for the arginine deprivation treatment of L-arginine auxotrophic tumors. The optimum pH of ADI varies significantly, yet little is known about the origin of this variety. Here, Pseudomonas aeruginosa ADI (PaADI), an enzyme that functions only at acidic pH, was ut...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2012-0183
更新日期:2012-09-01 00:00:00
abstract::We present a method that allows preparing long DNA containing defined mismatches without the use of gel electrophoretic or chromatographic purification steps. The preparation starts with the synthesis of two PCR products, which are identical except for those positions that will later form the mismatches. One of the PC...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2002.166
更新日期:2002-09-01 00:00:00
abstract::Bromelain isoinhibitors from pineapple stem (BIs) are unique double-chain inhibitors and inhibit the cysteine proteinase bromelain competitively. The three-dimensional structure was shown to be composed of two distinct domains, each of which is formed by a three-stranded anti-parallel beta-sheet. Unexpectedly, BIs wer...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2002.126
更新日期:2002-07-01 00:00:00
abstract::Chitinase-like proteins (CLPs) are lectins combining properties of cytokines and growth factors. Human CLPs include YKL-40, YKL-39 and SI-CLP that are secreted by cancer cells, macrophages, neutrophils, synoviocytes, chondrocytes and other cells. The best investigated CLP in cancer is YKL-40. Serum and plasma levels o...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2015-0269
更新日期:2016-03-01 00:00:00
abstract::We have investigated the methylation status of the repeat unit of the human ribosomal RNA genes in lung cancer. Using a Southern blot analysis approach we have determined that the non-transcribed region of these genes was generally heavily methylated, while the transcribed region was not methylated in either tumor or ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.1999.010
更新日期:1999-01-01 00:00:00
abstract::While the role of C2-ceramide in the induction of programmed cell death (PCD) in animal systems has been well documented, little is known of its role in plant cells. Here we show that C2-ceramide induces PCD in Arabidopsis suspension cultures, which is preceded by the generation of a calcium transient and an increase ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2005.020
更新日期:2005-02-01 00:00:00
abstract::A deletion mutant (V314) of the calcineurin A subunit was constructed using site-directed mutagenesis. Its phosphatase activity and function were then characterized. The V314 deletion significantly altered the phosphatase activity, which was more than ten times higher than that of wild-type calcineurin, the calcineuri...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.1999.163
更新日期:1999-11-01 00:00:00
abstract::In this review, we discuss how time-resolved Fourier transform infrared (FTIR) spectroscopy is used to understand how GTP hydrolysis is catalyzed by small GTPases and their cognate GTPase-activating proteins (GAPs). By interaction with small GTPases, GAPs regulate important signal transduction pathways and transport m...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2014-0219
更新日期:2015-02-01 00:00:00
abstract::Rhomboids are ubiquitous intramembrane serine proteases that cleave transmembrane substrates. Their functions include growth factor signaling, mitochondrial homeostasis, and parasite invasion. A recent study revealed that the Escherichia coli rhomboid protease EcGlpG is essential for its extraintestinal pathogenic col...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2018-0255
更新日期:2018-11-27 00:00:00
abstract::In order to enhance and target gene delivery we have previously established a novel method, termed magnetofection, which uses magnetic force acting on gene vectors that are associated with magnetic particles. Here we review the benefits, the mechanism and the potential of the method with regard to overcoming physical ...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2003.082
更新日期:2003-05-01 00:00:00
abstract::Isolated human granulocyte plasma membranes contain progelatinase B. The binding of progelatinase B to the membrane, however, is relatively weak, and a considerable part of progelatinase B can be removed by simply washing the membrane with buffer. This detachment does not depend on the ionic strength of the buffer, in...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2000.007
更新日期:2000-01-01 00:00:00
abstract::Cysteine cathepsins are expressed in most tissues, including the gastrointestinal tract. We demonstrated an involvement of mouse intestinal cathepsin B in extracellular matrix remodeling for regeneration from trauma. The present study aimed at elucidating roles of cysteine cathepsins in the non-traumatized gastrointes...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2014-0151
更新日期:2014-10-01 00:00:00
abstract::92 kDa and 72 kDa gelatinases, two neutral proteinases exhibiting elastinolytic activity and secreted as zymogens by aortic smooth muscle cells, were shown to bind to insoluble elastin. The active form of each enzyme interacted with substrate more avidly than latent form. Once bound to insoluble elastin, 92 kDa progel...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/bchm.1997.378.3-4.265
更新日期:1997-03-01 00:00:00
abstract::Protein phosphatase 2A (PP2A) constitutes one of the major families of protein serine/threonine phosphatases found in all eukaryotic cells. PP2A holoenzymes are composed of a catalytic subunit complexed with a structural regulatory subunit of 65 kDa. These core subunits associate with regulatory subunits of various si...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.1999.139
更新日期:1999-09-01 00:00:00
abstract::Eukaryotic organisms have evolved complex and robust cellular stress response pathways to ensure maintenance of proteostasis and survival during fluctuating environmental conditions. Highly conserved stress response pathways can be triggered and coordinated at the cell-autonomous and cell-nonautonomous level by proteo...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2019-0385
更新日期:2020-08-27 00:00:00
abstract::Extreme thermophiles produce unusually long polyamines, including the linear caldopentamine (Cdp) and the branched pentamine tetrakis(3-aminopropyl)-ammonium (Taa), with the latter containing a central quaternary ammonium moiety. Here we compare the interaction of these two pentamines with RNA by studying the heat den...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2009.096
更新日期:2009-09-01 00:00:00
abstract::A kinetic study was performed of a model for an autocatalytic zymogen activation process involving both intra- and intermolecular routes, to which a chromogenic reaction in which the active enzyme acts upon one of its substrates was coupled to continuously monitor the reaction. Kinetic equations describing the evoluti...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2005.080
更新日期:2005-07-01 00:00:00
abstract::We studied the mode of regulation of the activity of mature cathepsin B (CB) by L-cysteine and some related thiols. The activity of CB with Z-Arg-Arg-NHMec as substrate was gradually inhibited over a range of increasing concentration of Cys, Cys methyl ester (CysOMe), Cys ethyl ester (CysOEt), N-acetyl-Cys (N-AcCys) a...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.1999.069
更新日期:1999-05-01 00:00:00
abstract::The bifunctional protein DCoH (dimerization cofactor of HNF-1) is a transcriptional coactivator of homeodomain proteins and a cytoplasmic enzyme. Here we report on the isolation, nucleotide sequence and genomic structure of the chicken and rat DCoH genes. They have an unusual first exon coding for a single amino acid....
journal_title:Biological chemistry
pub_type: 杂志文章
doi:
更新日期:1998-11-01 00:00:00
abstract::We have cloned from a chicken intestinal cDNA library Cmdr1, the first avian P-glycoprotein. Cmdr1 is 67% and 69% identical to proteins encoded by the human MDR1 and MDR2 genes, respectively. Functional expression of Cmdr1 in both mouse NIH 3T3 and yeast cells demonstrated that Cmdr1 represents the avian ortholog of h...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.1999.031
更新日期:1999-02-01 00:00:00
abstract::There are enzymatic and non-enzymatic mechanisms that generate NO* from nitrite in blood, stomach, saliva, urine and skin. In blood vessels, nitrite-derived NO* can provide protection via compensatory vasodilation during hypoxia, and in various body fluids it may have antibacterial activity. In the skin, nitrite-deriv...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2006.065
更新日期:2006-05-01 00:00:00
abstract::Recent reports presented contradictory results regarding the catabolism of mature atrial (ANP) and brain (BNP) natriuretic peptides in circulation. Especially the role of neutral endopeptidase (NEP) in BNP degradation was conversely discussed. Our present in vitro-studies characterize the NEP-dependent metabolism of A...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2004.036
更新日期:2004-02-01 00:00:00
abstract::In a recent study we showed that two proteinases (CMS2MS2 and CMS2MS3) from Carica candamarcensis enhance mammalian cell proliferation. The aim of the present study is the determination of the primary structure of CMS2MS2 and prediction of its three-dimensional structure. The protein contains 214 residues, including t...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2007.083
更新日期:2007-08-01 00:00:00
abstract::Thyroid hormones stimulate gap junctional communication in rat liver WB-F344 epithelial cells, elevating connexin43 mRNA and protein levels. In the present work we analysed connexin43 expression in liver and heart samples from thyroid hormone-treated Wistar rats. Connexin43 mRNA was elevated 2.1-fold in rat liver samp...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2000.120
更新日期:2000-09-01 00:00:00
abstract::Based on its monophenolic structure and given its pharmacological and toxicological importance, the ability of tyrosinase to oxidize acetaminophen was studied for the first time. Progress curves showed a transient phase characteristic of the monophenolase activity of tyrosinase prior to attaining the steady-state. The...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2002.217
更新日期:2002-12-01 00:00:00
abstract::The thyroglobulin type-1 (Tg-1) domain is a protein module that occurs in a variety of secreted and membrane proteins and is recognised as a potent inhibitor of cysteine peptidases. We present here some properties of the Tg-1 domain of human testican, a modularly organised proteoglycan secreted mainly by brain cells, ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2005.010
更新日期:2005-01-01 00:00:00
abstract::We have previously shown that short double-stranded RNAs of specific sequence induce phosphorylation in the activation loop of splicing variant D of the transforming growth factor beta-activated protein kinase 1 (TAK1-D). Here, we further characterize this novel function of TAK1-D and the mechanisms of this dsRNA-trig...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2009.046
更新日期:2009-05-01 00:00:00