Abstract:
:A deletion mutant (V314) of the calcineurin A subunit was constructed using site-directed mutagenesis. Its phosphatase activity and function were then characterized. The V314 deletion significantly altered the phosphatase activity, which was more than ten times higher than that of wild-type calcineurin, the calcineurin-immunosuppressant/immunophilin interaction, the effect of metal ions and calcineurin subunit interaction. We propose that the change of the activity and function of V314 is due to conformational changes of calcineurin to benefit the binding of, or stimulation by, Mn2+, or to affect the interaction between the A and B subunits.
journal_name
Biol Chemjournal_title
Biological chemistryauthors
Yan L,Wei Qdoi
10.1515/BC.1999.163keywords:
subject
Has Abstractpub_date
1999-11-01 00:00:00pages
1281-5issue
11eissn
1431-6730issn
1437-4315journal_volume
380pub_type
杂志文章abstract::Annexins are a highly conserved protein family that bind to phospholipids in a calcium (Ca2+) - dependent manner. Studies with purified annexins, as well as overexpression and knockdown approaches identified multiple functions predominantly linked to their dynamic and reversible membrane binding behavior. However, mos...
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pub_type: 杂志文章,评审
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journal_title:Biological chemistry
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journal_title:Biological chemistry
pub_type: 杂志文章,评审
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pub_type: 杂志文章
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journal_title:Biological chemistry
pub_type: 杂志文章
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journal_title:Biological chemistry
pub_type: 杂志文章,评审
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更新日期:2013-01-01 00:00:00
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journal_title:Biological chemistry
pub_type: 杂志文章,评审
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更新日期:2008-05-01 00:00:00
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journal_title:Biological chemistry
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更新日期:2019-03-26 00:00:00
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journal_title:Biological chemistry
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更新日期:2008-12-01 00:00:00
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journal_title:Biological chemistry
pub_type: 杂志文章
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更新日期:2004-07-01 00:00:00
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journal_title:Biological chemistry
pub_type: 杂志文章
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更新日期:2007-09-01 00:00:00
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journal_title:Biological chemistry
pub_type: 杂志文章,评审
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更新日期:2005-03-01 00:00:00
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journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2011-0209
更新日期:2012-03-01 00:00:00
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journal_title:Biological chemistry
pub_type: 杂志文章
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更新日期:2017-09-26 00:00:00