Abstract:
:In preeclampsia and hemolysis, elevated liver enzymes and low platelet (HELLP) syndrome, impaired trophoblast invasion and excessive fibrin deposition in the placental intervillous space is associated with fetal compromise. However, little information is available whether modulation of placental protease expression--potentially causing impaired trophoblast invasion--is associated with the HELLP syndrome. Total RNA and protein were extracted from placental tissue from 11 females with HELLP syndrome and 8 controls matched for gestational age. mRNA expression of matrix metalloprotease (MMP) -2 and -9, tissue inhibitors of metalloprotease (TIMP) -1, -2, and -3, and urokinase-type plasminogen activator receptor (uPAR) was determined by Northern blotting. Protein expression of MMP-2 and -9, and TIMP-1 and -2 was detected by Western blotting and that of uPA, uPAR, and plasminogen activator inhibitor (PAI) -1 by ELISA. In patients with HELLP syndrome, mRNA expression of MMP-2 and TIMP-2 was decreased, whereas TIMP-1 and -3 levels were unchanged. MMP-9 and uPAR mRNA was undetectable in both groups. Protein expression of all investigated proteolytic factors remained unchanged. Our findings at the mRNA level suggest a decrease in matrix remodeling in placentae from patients with HELLP syndrome compared with control pregnancies, although this is not supported at the protein level.
journal_name
Biol Chemjournal_title
Biological chemistryauthors
von Steinburg SP,Krüger A,Fischer T,Mario Schneider KT,Schmitt Mdoi
10.1515/BC.2009.123subject
Has Abstractpub_date
2009-11-01 00:00:00pages
1199-204issue
11eissn
1431-6730issn
1437-4315journal_volume
390pub_type
杂志文章abstract::Mammalian apolipoproteinB (apoB) RNA editing is a site-specific deamination reaction that mediates the C to U conversion responsible for apoB48 production in the mammalian small intestine. This process is not detected in chicken apoB RNA. Mammalian apoB RNA editing is mediated by a multicomponent enzyme complex that i...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/bchm.1998.379.8-9.1075
更新日期:1998-08-01 00:00:00
abstract::Animal cells release traces of material onto glass or silicon surfaces during adhesion and migration. This little studied phenomenon is a widespread and normal concomitant of cell migration. The paper introduces the study of such material. The traces can be visualised by different microscopic techniques (e.g. TIRF, IR...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/bchm.1998.379.8-9.1161
更新日期:1998-08-01 00:00:00
abstract::Abstract Fanconi anemia (FA) is a rare cancer-prone genetic disorder characterized by progressive bone marrow failure, chromosomal instability and redox abnormalities. There is much biochemical and genetic data, which strongly suggest that FA cells experience increased oxidative stress. The present study was designed ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2011.064
更新日期:2011-07-01 00:00:00
abstract::Interleukin-13 (IL-13) is a T-cell-derived pleiotropic cytokine of particular medical importance because of its critical role in the development of allergic asthma. The effects of IL-13 on its target cells are mediated through a dimeric transmembrane receptor (IL-13R), which shares the IL-4Ralpha subunit with the IL-4...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2007.036
更新日期:2007-03-01 00:00:00
abstract::Invadolysin is a novel metalloprotease conserved amongst metazoans that is essential for life in Drosophila. We previously showed that invadolysin was essential for the cell cycle and cell migration, linking to metabolism through a role in lipid storage and interaction with mitochondrial proteins. In this study we dem...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2016-0226
更新日期:2017-03-01 00:00:00
abstract::A water-soluble [18O]-labeled endoperoxide derived from N,N'-di(2,3-dihydroxypropyl)-1,4-naphthalene-dipropanamide (DHPN18O2) has been shown to act as a clean chemical source of [18O]-labeled molecular singlet oxygen. This allows the assessment of the singlet oxygen (1O2) reactivity toward biological targets such as D...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2002.063
更新日期:2002-03-01 00:00:00
abstract::We present a rapid and simple method for the sexing of human and other primate DNA. Homologous introns from the X- and Y-linked zinc finger protein genes (ZFX and ZFY) are PCR amplified with one primer pair. An Alu element insertion in the ZFX intron results in a size difference in the amplicons which is resolved by a...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:
更新日期:1998-10-01 00:00:00
abstract::As a crucial protein, the herpes simplex virus 1 (HSV-1) UL2 protein has been shown to take part in various stages of viral infection, nonetheless, its exact subcellular localization and transport molecular determinants are not well known thus far. In the present study, by using live cells fluorescent microscopy assay...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2016-0268
更新日期:2017-04-01 00:00:00
abstract::Human kallikrein-related peptidases (KLKs), including the well-known prostate cancer biomarker, prostate-specific antigen (PSA-KLK3), along with 14 other serine proteinase KLK family members are now known to regulate cells by cleaving and activating members of the G-protein-coupled proteinase-activated receptor (PAR) ...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2014-0123
更新日期:2014-09-01 00:00:00
abstract::A kinetic study was performed of a model for an autocatalytic zymogen activation process involving both intra- and intermolecular routes, to which a chromogenic reaction in which the active enzyme acts upon one of its substrates was coupled to continuously monitor the reaction. Kinetic equations describing the evoluti...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2005.080
更新日期:2005-07-01 00:00:00
abstract::Isolated human granulocyte plasma membranes contain progelatinase B. The binding of progelatinase B to the membrane, however, is relatively weak, and a considerable part of progelatinase B can be removed by simply washing the membrane with buffer. This detachment does not depend on the ionic strength of the buffer, in...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2000.007
更新日期:2000-01-01 00:00:00
abstract::The GAGA box of the rat serine protease inhibitor 2 (spi 2) genes not only acts as a basal promoter element, but also mediates transcriptional activation by growth hormone and interleukin-6. The GAGA box is separated from the TATA box by only 12 bp, and this close association is required for efficient transcription. H...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2000.127
更新日期:2000-11-01 00:00:00
abstract::Fluorescence correlation spectroscopy (FCS) was used in monitoring human parvovirus B19 virus-like particle (VLP) antibody complexes from acute phase and past-immunity serum samples. The Oregon Green 488-labeled VLPs gave an average diffusion coefficient of 1.7 x 10(-7) cm2 s(-1) with an apparent hydrodynamic radius o...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2004.011
更新日期:2004-01-01 00:00:00
abstract::The role of Zn2+-sensing receptor GPR39 on glucose homeostasis and incretin regulation was assessed in enteroendocrine L- and K-cells. Anti-hyperglycaemic, insulinotropic and incretin secreting properties of Zn2+ were explored in normal, diabetic and incretin receptor knockout mice. Compared to intraperitoneal injecti...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2018-0393
更新日期:2019-06-20 00:00:00
abstract::Mitochondria are indispensable cellular organelles providing ATP and numerous other essential metabolites to ensure cell survival. Reactive oxygen species (ROS), which are formed as side reactions during oxidative phosphorylation or by external agents, induce molecular damage in mitochondrial proteins, lipids/membrane...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2017-0206
更新日期:2017-12-20 00:00:00
abstract::The bifunctional protein DCoH (dimerization cofactor of HNF-1) is a transcriptional coactivator of homeodomain proteins and a cytoplasmic enzyme. Here we report on the isolation, nucleotide sequence and genomic structure of the chicken and rat DCoH genes. They have an unusual first exon coding for a single amino acid....
journal_title:Biological chemistry
pub_type: 杂志文章
doi:
更新日期:1998-11-01 00:00:00
abstract::Acid ceramidase (N-acylsphingosine deacylase, EC 3.5.1.23; AC) is the lipid hydrolase responsible for the degradation of ceramide into sphingosine and free fatty acids within lysosomes. The enzymatic activity was first identified over four decades ago and is deficient in two rare inherited disorders, Farber lipogranul...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2014-0290
更新日期:2015-06-01 00:00:00
abstract::Bauhinia ungulata factor Xa inhibitor (BuXI) inactivates factor Xa and LOPAP, a prothrombin activator proteinase isolated from the venom of Lonomia obliqua caterpillar bristles. The reactive site of the enzyme-inhibitor interaction was explored to design specific substrates for both enzymes. Methionine is crucial for ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2003.055
更新日期:2003-03-01 00:00:00
abstract::The ubiquitous calpains, mu- and m-calpain, have been implicated in essential physiological processes and various pathologies. Cell-permeable specific inhibitors are important tools to elucidate the roles of calpains in cultivated cells and animal models. The synthetic N-acetylated 27-mer peptide derived from exon B o...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2003.045
更新日期:2003-03-01 00:00:00
abstract::The cell, as a molecular system, is often interpreted in terms of complex clockworks, and the design charts of mechanical and electrical engineering are assumed to provide adequate approximations for the description of cellular organization. However, a growing body of experimental evidence obtained through the observa...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2005.030
更新日期:2005-03-01 00:00:00
abstract::Dipeptidyl peptidase-IV has been demonstrated to play a role in cancer biology by many authors. Since then, additional proteins possessing similar enzymatic activity have been described and their role in cancerogenesis has been hypothesized. To assess the complexity of these 'Dipeptidyl peptidase-IV activity and/or st...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2004.067
更新日期:2004-06-01 00:00:00
abstract::MHC (major histocompatibility complex) class I molecules bind intracellular virus-derived peptides in the endoplasmic reticulum (ER) and present them at the cell surface to cytotoxic T lymphocytes. Peptide-free class I molecules at the cell surface, however, could lead to aberrant T cell killing. Therefore, cells ensu...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2004.100
更新日期:2004-09-01 00:00:00
abstract::γ-Secretase is a membrane-embedded protease complex with presenilin as the catalytic component. Cleavage within the transmembrane domain of the amyloid β-protein precursor (APP) by γ-secretase produces the C-terminus of the amyloid β-peptide (Aβ), a proteolytic product prone to aggregation and strongly linked to Alzhe...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2012-0140
更新日期:2012-09-01 00:00:00
abstract::We developed a novel method for the simultaneous extraction and analysis of total tissue RNA and DNA to quantify the RNA and DNA oxidation products 8-oxo-7,8-dihydroguanosine and 8-oxo-7,8-dihydro-2'-deoxyguanosine using HPLC coupled to electrochemical detection (HPLC-ECD). The protein denaturing agents guanidine thio...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2006.014
更新日期:2006-01-01 00:00:00
abstract::Bone morphogenetic proteins (BMP) are dimeric factors initiating several distinct signaling cascades by binding to two types of transmembrane serine/threonine kinase receptors (BRI and BRII), and are thus regulating several steps in embryonal development and adult tissue homeostasis. BMP-2 contains two symmetrical pai...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2001.149
更新日期:2001-08-01 00:00:00
abstract::The signal recognition particle (SRP), a ubiquitous cytoplasmic ribonucleoprotein particle, plays an essential role in promoting co-translational translocation of proteins into the endoplasmic reticulum. Here, we summarise recent progress made in the understanding of two essential SRP functions: the signal recognition...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.1999.021
更新日期:1999-02-01 00:00:00
abstract::Recent progress in the identification and partial characterization of novel genes encoding cysteine proteases of the papain family has considerably increased our knowledge of this family of enzymes. Kinetic data available to date for this large family indicate relatively broad, overlapping specificities for most enzym...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2003.094
更新日期:2003-06-01 00:00:00
abstract::N-acyl homoserine lactones (AHLs) are quorum sensing (QS) signal molecules used by most Gram-negative pathogenic bacteria. In this article the lactonase activity of the preparations based on hexahistidine-tagged organophosphorus hydrolase (His6-OPH) towards AHLs was studied. Initially, three of the most interesting β-...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2018-0162
更新日期:2018-07-26 00:00:00
abstract::Heparan sulfate proteoglycans are obligatory for receptor binding and mitogenic activity of the basic fibroblast growth factor (bFGF). In the present study the influence of undersulfated heparan sulfate on the expression of basic fibroblast growth factor and coronary smooth muscle cell (cSMC) proliferation was investi...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/bchm.1997.378.7.701
更新日期:1997-07-01 00:00:00
abstract::Interleukin-1 signaling is initiated by recruitment of adapter proteins and kinases to the type I interleukin-1 receptor (IL-1RI). It is modulated by accompanying redox processes at various levels, such as (auto-) phosphorylation of the IL-1RI-associated kinase IRAK, the phosphorylation of IkappaB and translocation an...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2003.068
更新日期:2003-04-01 00:00:00