Abstract:
:A water-soluble [18O]-labeled endoperoxide derived from N,N'-di(2,3-dihydroxypropyl)-1,4-naphthalene-dipropanamide (DHPN18O2) has been shown to act as a clean chemical source of [18O]-labeled molecular singlet oxygen. This allows the assessment of the singlet oxygen (1O2) reactivity toward biological targets such as DNA. The present work focuses on the qualitative identification of the main 1O2-oxidation products of 8-oxo-7,8-dihydro-2'-deoxyguanosine, which was achieved using high performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS). Thus, the [18O]-labeled and unlabeled imidazolone and oxazolone, together with the diastereoisomeric spiroiminodihydantoin nucleosides, were detected as the main degradation products. In addition, a modified nucleoside that exhibits similar features as those of the oxidized guanidinohydantoin molecule was detected. Our data strongly suggest that the imidazolone and oxazolone nucleosides are generated via the rearrangement of an unstable 5-hydroperoxide intermediate. Interestingly, the combined use of appropriate tools, including isotopically labeled singlet oxygen and the high- resolution HPLC-ESI-MS/MS technique, has allowed to shed new light on the 1O2-mediated oxidation reactions of guanine DNA components.
journal_name
Biol Chemjournal_title
Biological chemistryauthors
Martinez GR,Medeiros MH,Ravanat JL,Cadet J,Di Mascio Pdoi
10.1515/BC.2002.063keywords:
subject
Has Abstractpub_date
2002-03-01 00:00:00pages
607-17issue
3-4eissn
1431-6730issn
1437-4315journal_volume
383pub_type
杂志文章abstract::Stromal cell-derived factor-1alpha (SDF-1alpha) is a strong migratory stimulant for hematopoietic stem and progenitor cells (HSPCs). The hematopoietic cytokines thrombopoietin (TPO), Flt3-ligand (FL), stem cell factor (SCF) and interleukin 11 (IL-11) are able to stimulate amplification of primitive murine hematopoieti...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2008.099
更新日期:2008-07-01 00:00:00
abstract::The average amount of cholesterol in the whole animal equals approximately 2100 mg/kg body weight, and 15% and 23% of this sterol in the mouse and human, respectively, is found in the central nervous system. There is no detectable uptake across the blood-brain barrier of cholesterol carried in lipoproteins in the plas...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2009.035
更新日期:2009-04-01 00:00:00
abstract::The ubiquitous calpains, mu- and m-calpain, have been implicated in essential physiological processes and various pathologies. Cell-permeable specific inhibitors are important tools to elucidate the roles of calpains in cultivated cells and animal models. The synthetic N-acetylated 27-mer peptide derived from exon B o...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2003.045
更新日期:2003-03-01 00:00:00
abstract::Silver nanoparticles (AgNPs) have many biological applications in biomedicine, biotechnology and other life sciences. Depending on the size, shape and the type of carrier, AgNPs demonstrate different physical and chemical properties. AgNPs have strong antimicrobial, antiviral and antifungal activity, thus they are use...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2012-0202
更新日期:2013-01-01 00:00:00
abstract::Plasma kallikrein plays a role in coagulation, fibrinolysis and inflammation. Cathepsins B and L participate in (patho)physiological processes such as peptide antigen processing, tissue remodeling events, protein turnover in cells, hormone processing and tumor invasion. The present work analyzes the processing of prek...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2004.141
更新日期:2004-11-01 00:00:00
abstract::The purpose of this work was to study the regulative role of the glutamate receptor found earlier in the brain mitochondria. In the present work a glutamate-dependent signaling system with similar features was detected in mitochondria of the heart. The glutamate-dependent signaling system in the heart mitochondria was...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2015-0289
更新日期:2016-05-01 00:00:00
abstract::Recent progress in the identification and partial characterization of novel genes encoding cysteine proteases of the papain family has considerably increased our knowledge of this family of enzymes. Kinetic data available to date for this large family indicate relatively broad, overlapping specificities for most enzym...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2003.094
更新日期:2003-06-01 00:00:00
abstract::Annexins are a highly conserved protein family that bind to phospholipids in a calcium (Ca2+) - dependent manner. Studies with purified annexins, as well as overexpression and knockdown approaches identified multiple functions predominantly linked to their dynamic and reversible membrane binding behavior. However, mos...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2016-0168
更新日期:2016-10-01 00:00:00
abstract::The 15 human kallikrein-related peptidases (KLKs) are clinically important biomarkers and therapeutic targets of interest in inflammation, cancer, and neurodegenerative disease. KLKs are secreted as inactive pro-forms (pro-KLKs) that are activated extracellularly by specific proteolytic release of their amino-terminal...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2012-0249
更新日期:2013-01-01 00:00:00
abstract::Malaria can be a very severe disease, particularly in young children, pregnant women (mostly in primipara), and malaria naïve adults, and currently ranks among the most prevalent infections in tropical and subtropical areas throughout the world. The widespread occurrence and the increased incidence of malaria in many ...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2001.069
更新日期:2001-04-01 00:00:00
abstract::The human copper exporters ATP7A and ATP7B contain domains common to all P-type ATPases as well as class-specific features such as six sequential heavy-metal binding domains (HMBD1-HMBD6) and a type-specific constellation of transmembrane helices. Despite the medical significance of ATP7A and ATP7B related to Menkes a...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2011-0249
更新日期:2012-04-01 00:00:00
abstract::We developed a novel method for the simultaneous extraction and analysis of total tissue RNA and DNA to quantify the RNA and DNA oxidation products 8-oxo-7,8-dihydroguanosine and 8-oxo-7,8-dihydro-2'-deoxyguanosine using HPLC coupled to electrochemical detection (HPLC-ECD). The protein denaturing agents guanidine thio...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2006.014
更新日期:2006-01-01 00:00:00
abstract::Eukaryotic organisms have evolved complex and robust cellular stress response pathways to ensure maintenance of proteostasis and survival during fluctuating environmental conditions. Highly conserved stress response pathways can be triggered and coordinated at the cell-autonomous and cell-nonautonomous level by proteo...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2019-0385
更新日期:2020-08-27 00:00:00
abstract::As an important protein, UL31 has been demonstrated to play multiple roles in herpes simplex virus 1 (HSV-1) replication. Previous studies showed that UL31 predominantly locates in the nucleus in chemical fixed cells and live cells, however, the determining mechanisms for its nuclear translocation is not clear. In the...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2015-0299
更新日期:2016-06-01 00:00:00
abstract::Small-angle scattering (SAS) of X-rays and neutrons reveals low-resolution structures of biological macromolecules in solution. With the recent experimental and methodological advances, SAS became a unique tool for characterising biological systems. The method covers an extremely broad range of molecule sizes (from a ...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2010.093
更新日期:2010-07-01 00:00:00
abstract::G-protein coupled receptor 119 (GPR119) is emerging as a potential target for the treatment of type 2 diabetes with beneficial effects on glucose homeostasis. This study assessed the insulin-secreting properties of various GPR119 agonists and the distribution of GPR119 in pancreatic islets. Endogenous ligands [oleoyle...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2013-0255
更新日期:2014-04-01 00:00:00
abstract::DHX36 is a member of the DExD/H box helicase family, which comprises a large number of proteins involved in various cellular functions. Recently, the function of DHX36 in the regulation of G-quadruplexes (G4s) was demonstrated. G4s are alternative nucleic acid structures, which influence many cellular pathways on a tr...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2020-0292
更新日期:2020-10-01 00:00:00
abstract::Metarhizium anisopliae is an entomopathogenic fungus relevant in biotechnology with applications like malaria vector control. Studies of its virulence factors are therefore of great interest. Fungal ribotoxins are toxic ribonucleases with extraordinary efficiency against ribosomes and suggested as potential insecticid...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2016-0119
更新日期:2017-01-01 00:00:00
abstract::Protein transport, especially into different cellular compartments, is a highly coordinated and regulated process. The molecular machineries which carry out these transport processes are highly complex in structure, function, and regulation. In the case of chloroplasts, thousands of protein molecules have been estimat...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2009.051
更新日期:2009-08-01 00:00:00
abstract::Meprins are zinc-endopeptidases of the astacin family, which are expressed as membrane-bound or secreted forms in renal and intestinal brush-border membranes of mouse, rat and man. There are two types of meprin subunits, alpha and beta, which form disulfide-bonded homo- and heterodimers; further oligomerization is med...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2003.092
更新日期:2003-05-01 00:00:00
abstract::As a crucial protein, the herpes simplex virus 1 (HSV-1) UL2 protein has been shown to take part in various stages of viral infection, nonetheless, its exact subcellular localization and transport molecular determinants are not well known thus far. In the present study, by using live cells fluorescent microscopy assay...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2016-0268
更新日期:2017-04-01 00:00:00
abstract::Cancer cells are relatively resistant to endoplasmic reticulum (ER) stress-induced apoptosis. However, the underlying mechanisms remain largely unclear. We observed that the microRNAs miR-221/222 are associated with apoptosis regulation under ER stress in human hepatocellular carcinoma (HCC) cells. Induction of ER str...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2010.072
更新日期:2010-07-01 00:00:00
abstract::The members of the YidC/Oxa1/Alb3 protein family are evolutionary conserved in all three domains of life. They facilitate the insertion of membrane proteins into bacterial, mitochondrial, and thylakoid membranes and have been implicated in membrane protein folding and complex formation. The major classes of substrates...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2012-0199
更新日期:2012-11-01 00:00:00
abstract::The solute carrier family SLC10 consists of seven members, including the bile acid transporters Na+/taurocholate co-transporting polypeptide (NTCP) and apical sodium-dependent bile acid transporter (ASBT), the steroid sulfate transporter SOAT as well as four orphan carriers (SLC10A3, SLC10A4, SLC10A5 and SLC10A7). Pre...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2019-0148
更新日期:2019-09-25 00:00:00
abstract::The hypoxia-inducible factor-1 (HIF-1) is a basic-helix-loop-helix (bHLH) heterodimeric transcription factor activated by reductions in oxygen concentration (hypoxia). Activated HIF-1 upregulates expression of genes involved in the adaptation of higher organisms to hypoxic conditions, caused by e.g. high altitude, ane...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:
更新日期:1997-07-01 00:00:00
abstract::Coxsackievirus B3 (CVB-3) is a plus-strand RNA virus that is believed to be the most common causal agent of viral myocarditis. Since no specific treatment for CVB-3 infections is available to date, we and others have recently started to develop RNA interference (RNAi) approaches to prevent virus propagation. Here we d...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2005.100
更新日期:2005-09-01 00:00:00
abstract::Neuritic plaques of Alzheimer patients are composed of multiple protein components. Among them, the amyloid beta-peptides (Abeta) 1-40/42 and further N- and C-terminally modified fragments of Abeta are highly abundant. Most prominent are the isoaspartate (isoAsp)-Abeta peptides and pyroglutamyl (pGlu)-Abeta. While pGl...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2008.125
更新日期:2008-08-01 00:00:00
abstract::PAR and C/EBP family proteins are liver-enriched basic leucine zipper (bZip) transcription factors that bind similar sites on the promoters of albumin and cholesterol 7 alpha hydroxylase genes. However, C/EBP proteins have a more relaxed binding specificity than PAR proteins, in that they recognize many sites within p...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:
更新日期:1996-12-01 00:00:00
abstract::Small RNAs such as microRNAs (miRNAs), short interfering RNAs (siRNAs) or Piwi-interacting RNAs (piRNAs) are important regulators of gene expression in various organisms. Small RNAs bind to a member of the Argonaute protein family and are incorporated into larger structures that mediate diverse gene silencing events. ...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2014-0116
更新日期:2014-06-01 00:00:00
abstract::MHC (major histocompatibility complex) class I molecules bind intracellular virus-derived peptides in the endoplasmic reticulum (ER) and present them at the cell surface to cytotoxic T lymphocytes. Peptide-free class I molecules at the cell surface, however, could lead to aberrant T cell killing. Therefore, cells ensu...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2004.100
更新日期:2004-09-01 00:00:00