Zinc-induced activation of GPR39 regulates glucose homeostasis through glucose-dependent insulinotropic polypeptide secretion from enteroendocrine K-cells.

abstract：:The three isoforms of human Ins(1,4,5)P3 3-kinase (IP3K) show remarkable differences in their intracellular targeting. Whereas predominant targeting to the cytoskeleton and endoplasmic reticulum has been shown for IP3K-A and IP3K-B, rat IP3K-C shuttles actively between the nucleus and cytoplasm. In the present study w...

journal_title：Biological chemistry

authors： Nalaskowski MM,Windhorst S,Stockebrand MC,Mayr GW

更新日期：2006-05-01 00:00:00

abstract：:DEAD-box proteins disrupt or remodel RNA and protein/RNA complexes at the expense of ATP. The catalytic core is composed of two flexibly connected RecA-like domains. The N-terminal domain contains most of the motifs involved in nucleotide binding and serves as a minimalistic model for helicase/nucleotide interactions....

journal_title：Biological chemistry

authors： Strohmeier J,Hertel I,Diederichsen U,Rudolph MG,Klostermeier D

更新日期：2011-04-01 00:00:00

abstract：:The efficient execution of apoptotic cell death with the clearance of apoptotic debris by phagocytes is a key regulatory mechanism ensuring tissue homeostasis. Failure in this execution program contributes to the pathogenesis of many human diseases. In this review, we describe the current knowledge regarding the inter...

journal_title：Biological chemistry

authors： Weigert A,Jennewein C,Brüne B

更新日期：2009-05-01 00:00:00

abstract：:Abstract Hairpin ribozymes occur naturally only in the satellite RNAs of tobacco ringspot virus (TRsV), chicory yellow mottle virus (CYMoV) and arabis mosaic virus (ArMV). The catalytic centre of the predominantly studied sTRsV hairpin ribozyme, and of sArMV is organised around a four-way helical junction. We show her...

journal_title：Biological chemistry

authors： Bajaj P,Steger G,Hammann C

更新日期：2011-07-01 00:00:00

abstract：:The mitochondrial DNA (mtDNA) encompasses two classes of functionally important sequence variants: recent pathogenic mutations and ancient adaptive polymorphisms. To rapidly and cheaply evaluate both classes of single nucleotide variants (SNVs), we have developed an integrated system in which mtDNA SNVs are analyzed b...

journal_title：Biological chemistry

authors： Poole JC,Procaccio V,Brandon MC,Merrick G,Wallace DC

更新日期：2010-10-01 00:00:00

abstract：:Isolated human granulocyte plasma membranes contain progelatinase B. The binding of progelatinase B to the membrane, however, is relatively weak, and a considerable part of progelatinase B can be removed by simply washing the membrane with buffer. This detachment does not depend on the ionic strength of the buffer, in...

journal_title：Biological chemistry

authors： Kolkenbrock H,Zimmermann J,Burmester GR,Ulbrich N

更新日期：2000-01-01 00:00:00

abstract：:We have previously shown that compatible organic osmolytes, such as betaine, myo-inositol and taurine, are part of the stress response of normal human keratinocytes (NHKs) to ultraviolet B (UVB) radiation. In this regard, we tested human HaCaT keratinocytes as a surrogate cell line for NHK. HaCaT cells osmo-dependentl...

journal_title：Biological chemistry

authors： Warskulat U,Brookmann S,Reinen A,Häussinger D

更新日期：2007-12-01 00:00:00

abstract：:γ-Secretase is a membrane-embedded protease complex with presenilin as the catalytic component. Cleavage within the transmembrane domain of the amyloid β-protein precursor (APP) by γ-secretase produces the C-terminus of the amyloid β-peptide (Aβ), a proteolytic product prone to aggregation and strongly linked to Alzhe...

journal_title：Biological chemistry

authors： Wolfe MS

更新日期：2012-09-01 00:00:00

abstract：:The relationship between the structure and activity of meta- and para-hydroxylated monophenols was studied during their tyrosinase-catalysed hydroxylation and the rate-limiting steps of the reaction mechanism were identified. The para-hydroxylated substrates permit us to study the effect of a substituent (R) in the ca...

journal_title：Biological chemistry

authors： Fenoll LG,Rodríguez-López JN,Varón R,García-Ruiz PA,García-Cánovas F,Tudela J

更新日期：2000-04-01 00:00:00

abstract：:Cells need to process multifaceted external cues to steer their dynamic behavior. To efficiently perform this task, cells implement several exploratory mechanisms to actively sample their environment. In particular, cells can use exploratory actin-based cell protrusions and contractions to engage and squeeze the envir...

journal_title：Biological chemistry

authors： Nalbant P,Dehmelt L

更新日期：2018-07-26 00:00:00

abstract：:Mutations of the p53 gene may alter the specific regulatory domains of the protein. We examined the conserved domains III, IV and V by SSCP using PCR primers covering exons 5, 6, 7 and 8 from hairy cell leukemia (HCL), polyps, colorectal and gastric carcinomas. A low rate of p53 mutations was detected in HCL and polyp...

journal_title：Biological chemistry

authors： Marcsek ZL,König EA

更新日期：1998-04-01 00:00:00

abstract：:Molecular chaperones of the heat shock protein 70 (Hsp70) family play a crucial role in the presentation of exogenous antigenic peptides by antigen-presenting cells (APCs). In a combined biochemical and immunological approach, we characterize the biochemical interaction of tumor-associated peptides with human Hsp70 an...

journal_title：Biological chemistry

authors： Pandya MJ,Bendz H,Manzenrieder F,Noessner E,Kessler H,Buchner J,Issels RD

更新日期：2009-04-01 00:00:00

abstract：:The HIRIP3 protein had been identified from its interaction with the HIRA histone chaperone. Experiments using anti-peptide antisera indicated that this 556-aa protein is nuclear throughout the cell cycle and excluded from condensed chromatin during mitosis. Based on its electrophoretic migration and sensitivity to ph...

journal_title：Biological chemistry

authors： Assrir N,Filhol O,Galisson F,Lipinski M

更新日期：2007-04-01 00:00:00

abstract：:Aberrant microglial activation and neuroinflammation is a pathological hallmark of amyotrophic lateral sclerosis (ALS). Fractalkine (CX3CL1) is mostly expressed on neuronal cells. The fractalkine receptor (CX3CR1) is predominantly expressed on microglia. Many progressive neuroinflammatory disorders show disruption of ...

journal_title：Biological chemistry

authors： Liu C,Hong K,Chen H,Niu Y,Duan W,Liu Y,Ji Y,Deng B,Li Y,Li Z,Wen D,Li C

更新日期：2019-04-24 00:00:00

abstract：:The formation of N-glycosidic linkages of glycoproteins involves the ordered assembly of the common Glc3Man9GlcNAc2 core-oligosaccharide on the lipid carrier dolichyl pyrophosphate. Whereas early mannosylation steps occur on the cytoplasmic side of the endoplasmic reticulum with GDP-Man as donor, the final reactions f...

journal_title：Biological chemistry

authors： Sharma CB,Knauer R,Lehle L

更新日期：2001-02-01 00:00:00

abstract：:Hypoxia-inducible transcription factor (HIF), an αβ dimer, is the key inducer of hypoxia-responsive genes that operate both during normal development and pathological processes in association with decreased oxygen availability. The products of HIF target genes function in, e.g., hematopoiesis, angiogenesis, iron trans...

journal_title：Biological chemistry

authors： Myllyharju J,Koivunen P

更新日期：2013-04-01 00:00:00

abstract：:Glucose-dependent insulinotropic polypeptide (GIP) is an incretin hormone secreted by endocrine K-cells in response to nutrient absorption. In this study we have utilized a specific and enzymatically stable GIP receptor antagonist, (Pro3)GIP, to evaluate the contribution of endogenous GIP to insulin secretion and gluc...

journal_title：Biological chemistry

authors： Irwin N,Gault VA,Green BD,Greer B,McCluskey JT,Harriott P,O'Harte FP,Flatt PR

更新日期：2004-09-01 00:00:00

abstract：:The GAGA box of the rat serine protease inhibitor 2 (spi 2) genes not only acts as a basal promoter element, but also mediates transcriptional activation by growth hormone and interleukin-6. The GAGA box is separated from the TATA box by only 12 bp, and this close association is required for efficient transcription. H...

journal_title：Biological chemistry

authors： Leverrier S,Cinato E,Paul C,Derancourt J,Bemark M,Leanderson T,Legraverend C

更新日期：2000-11-01 00:00:00

abstract：:Extreme thermophiles produce unusually long polyamines, including the linear caldopentamine (Cdp) and the branched pentamine tetrakis(3-aminopropyl)-ammonium (Taa), with the latter containing a central quaternary ammonium moiety. Here we compare the interaction of these two pentamines with RNA by studying the heat den...

journal_title：Biological chemistry

authors： Hayrapetyan A,Grosjean H,Helm M

更新日期：2009-09-01 00:00:00

abstract：:The pancreatic β-cell surface protein Tmem27 is promotes the preservation of functional β-cell mass. It is a selective substrate of the protease Bace2, yet the intramolecular features of Tmem27 that regulate its processing by this sheddase have not been characterized. In particular, the importance of homodimerization,...

journal_title：Biological chemistry

authors： Esterházy D,Akpinar P,Stoffel M

更新日期：2012-05-01 00:00:00

abstract：:Bacterial infections often upregulate the plasma kinin-forming cascade of the host (the 'contact system') which is triggered by adsorption of high molecular mass kininogen (HK), coagulation factor XII (FXII) and prekallikrein (pHPK) on the host or pathogen cell surfaces. A possible activation of the contact system upo...

journal_title：Biological chemistry

authors： Karkowska-Kuleta J,Kozik A,Rapala-Kozik M

更新日期：2010-01-01 00:00:00

abstract：:Vascular injury triggers endothelial exocytosis of granules, releasing pro-inflammatory and pro-thrombotic mediators into the blood. Nitric oxide (NO) and reactive oxygen species (ROS) limit vascular inflammation and thrombosis by inhibiting endothelial exocytosis. NO decreases exocytosis by regulating the activity of...

journal_title：Biological chemistry

authors： Lowenstein CJ,Tsuda H

更新日期：2006-10-01 00:00:00

abstract：:The enzymatic properties of glycosylphosphatidylinositol-specific phospholipase D (EC 3.1.4.50) were characterized using a 6,000-fold purified enzyme. This was obtained in 100 microg amounts from human serum with a recovery of 35%. Pure alkaline phosphatase containing one anchor moiety per molecule was used as substra...

journal_title：Biological chemistry

authors： Rhode H,Schulze M,Cumme GA,Göhlert A,Blume E,Bublitz R,Schilling K,Horn A

更新日期：2000-05-01 00:00:00

abstract：:We have investigated the methylation status of the repeat unit of the human ribosomal RNA genes in lung cancer. Using a Southern blot analysis approach we have determined that the non-transcribed region of these genes was generally heavily methylated, while the transcribed region was not methylated in either tumor or ...

journal_title：Biological chemistry

authors： Shiraishi M,Sekiguchi A,Chuu YH,Sekiya T

更新日期：1999-01-01 00:00:00

abstract：:The surface topography of red blood cells (RBCs) was investigated under near-physiological conditions using atomic force microscopy (AFM). An immobilization protocol was established where RBCs are coupled via molecular bonds of the membrane glycoproteins to wheat germ agglutinin (WGA), which is covalently and flexibly...

journal_title：Biological chemistry

authors： Kamruzzahan AS,Kienberger F,Stroh CM,Berg J,Huss R,Ebner A,Zhu R,Rankl C,Gruber HJ,Hinterdorfer P

更新日期：2004-10-01 00:00:00

abstract：:Hypochlorous acid (HOCl) is a bactericidal compound formed by activated neutrophils during inflammation. Overproduction of HOCl causes damage to tissues at the site of neutrophil accumulation. The deleterious effects of excessive HOCl formation can be attenuated using antioxidants. Thiols and thioethers are known to b...

journal_title：Biological chemistry

authors： den Hartog GJ,Haenen GR,Vegt E,van der Vijgh WJ,Bast A

更新日期：2002-03-01 00:00:00

abstract：:The regulation of eukaryotic gene expression is a complicated process involving the interaction of a large number of transacting factors with specific cis-regulatory elements. DNA methylation plays a role in this scheme by acting in cis to modulate protein-DNA interactions. Several lines of evidence indicate that meth...

journal_title：Biological chemistry

authors： Bergman Y,Mostoslavsky R

更新日期：1998-04-01 00:00:00

abstract：:In order to identify regulatory elements involved in the hepatocyte specific expression of the enzyme glutamine synthetase [GS (E.C. 6.3.1.2)] we analyzed the first intron of the rat GS gene. A sequence analysis detected clusters of potential transcription factor binding sites in regions that are hypersensitive for DN...

journal_title：Biological chemistry

authors： Gaunitz F,Gaunitz C,Papke M,Gebhardt R

更新日期：1997-01-01 00:00:00

abstract：:In this study, we tested the biological activity of a novel acylated form of (Pro3)glucose-dependent insulinotropic polypetide [(Pro3)GIP] prepared by conjugating palmitic acid to Lys16 to enhance its efficacy in vivo by promoting binding to albumin and extending its biological actions. Like the parent molecule (Pro3)...

journal_title：Biological chemistry

authors： Gault VA,Hunter K,Irwin N,Greer B,Green BD,Harriott P,O'Harte FP,Flatt PR

更新日期：2007-02-01 00:00:00

abstract：:We studied the mode of regulation of the activity of mature cathepsin B (CB) by L-cysteine and some related thiols. The activity of CB with Z-Arg-Arg-NHMec as substrate was gradually inhibited over a range of increasing concentration of Cys, Cys methyl ester (CysOMe), Cys ethyl ester (CysOEt), N-acetyl-Cys (N-AcCys) a...

journal_title：Biological chemistry

authors： Krepela E,Procházka J,Kárová B

更新日期：1999-05-01 00:00:00