Cis-regulatory sequences from the first intron of the rat glutamine synthetase gene are involved in hepatocyte specific expression of the enzyme.

abstract：:The hair follicle consists of a complex system of multiple tissue compartments that are clearly distinguishable by their morphology and type of differentiation. We have synthesized hair follicle-specific keratins from the companion layer (K6hf, K17) and the hair cortex (Ha1, Hb3, Hb6) in Escherichia coli. The assembly...

journal_title：Biological chemistry

authors： Hofmann I,Winter H,Mücke N,Langowski J,Schweizer J

更新日期：2002-09-01 00:00:00

abstract：:Five phage displayed peptide libraries were screened for binders to C1q, the recognition subunit of the classical complement pathway. Two rounds of panning resulted in the isolation and characterisation of several different phage displayed C1q-binding peptides from all five libraries. Two groups of the characterised p...

journal_title：Biological chemistry

authors： Lauvrak V,Brekke OH,Ihle O,Lindqvist BH

更新日期：1997-12-01 00:00:00

abstract：:Abstract The R- and K-gingipain proteases of Porphyromonas gingivalis are involved in proteolysis of haemoglobin from which the defensive dimeric haem pigment is formed. Whilst oxyhaemoglobin is refractory towards K-gingipain, methaemoglobin is rapidly degraded. Ligation of methaemoglobin with N3-, which effectively b...

journal_title：Biological chemistry

authors： Smalley JW,Birss AJ,Szmigielski B,Potempa J

更新日期：2008-09-01 00:00:00

abstract：:The role of Zn2+-sensing receptor GPR39 on glucose homeostasis and incretin regulation was assessed in enteroendocrine L- and K-cells. Anti-hyperglycaemic, insulinotropic and incretin secreting properties of Zn2+ were explored in normal, diabetic and incretin receptor knockout mice. Compared to intraperitoneal injecti...

journal_title：Biological chemistry

authors： Moran BM,Miskelly MG,Abdel-Wahab YHA,Flatt PR,McKillop AM

更新日期：2019-06-20 00:00:00

abstract：:Recent evidence suggests that epidermal growth factor receptor (EGFR)-dependent signaling contributes to liver cell proliferation, as well as to apoptotic liver cell death, and represents an important regulator of hepatic regeneration. This article summarizes recent findings on the molecular mechanisms involved in EGF...

journal_title：Biological chemistry

authors： Reinehr R,Häussinger D

更新日期：2009-10-01 00:00:00

abstract：:Host T cell activation, a key step in obtaining adaptive immunity against pathogens, is initiated by the binding of the T cell receptor to a foreign antigenic peptide presented by the major histocompatibility complex on the surface of an antigen-presenting cell and, consequently, formation of an immunological synapse....

journal_title：Biological chemistry

authors： Beyersdorf N,Müller N

更新日期：2015-06-01 00:00:00

abstract：:Recent reports presented contradictory results regarding the catabolism of mature atrial (ANP) and brain (BNP) natriuretic peptides in circulation. Especially the role of neutral endopeptidase (NEP) in BNP degradation was conversely discussed. Our present in vitro-studies characterize the NEP-dependent metabolism of A...

journal_title：Biological chemistry

authors： Walther T,Stepan H,Pankow K,Becker M,Schultheiss HP,Siems WE

更新日期：2004-02-01 00:00:00

abstract：:Eremophilanes are sesquiterpenes with a rearranged carbon skeleton formed both by plants and fungi, however, almost no plant eremophilanes are found in fungi. These eremophilanes possess mainly phytotoxic, antimicrobial, anticancer and immunomodulatory properties and in this review fungal eremophilanes with bioactivit...

journal_title：Biological chemistry

authors： Yuyama KT,Fortkamp D,Abraham WR

更新日期：2017-12-20 00:00:00

abstract：:Nitrogen dioxide (*NO2) is an oxidizing free radical which can initiate a variety of destructive pathways in living systems, and several diseases are suspected to be connected with both exogenously and endogenously formed *NO2. Peroxynitrite (ONOO-/ONOOH) is believed to be an important endogenous source of *NO2 radica...

journal_title：Biological chemistry

authors： Kirsch M,Korth HG,Sustmann R,de Groot H

更新日期：2002-03-01 00:00:00

abstract：:The influence of the cellular milieu, a complex and crowded solvent, is often neglected when biomolecular structure and function are studied in vitro. To mimic the cellular environment, crowding effects are commonly induced in vitro using artificial crowding agents like Ficoll or dextran. However, it is unclear if suc...

journal_title：Biological chemistry

authors： Gnutt D,Ebbinghaus S

更新日期：2016-01-01 00:00:00

abstract：:In order to study the role of N-glycans in the ER-associated degradation of unassembled immunoglobulin light (Ig L) chains, we introduced N-glycan acceptor sites into the variable domain of the murine Ig L chain kappaNS1, which is unfolded in unassembled molecules. We investigated the fate of kappaNS1 glycosylated at ...

journal_title：Biological chemistry

authors： Chillarón J,Adán C,Haas IG

更新日期：2000-12-01 00:00:00

abstract：:Studies of extracellular matrix (ECM) biology in the nervous system have mainly focused on laminin, fibronectin and tenascin-R, proteins that are present during nervous system development and normal function. However, during disease, fibrin, which physiologically is not present in the nervous tissue, is detected at ne...

journal_title：Biological chemistry

authors： Akassoglou K,Strickland S

更新日期：2002-01-01 00:00:00

abstract：:The kallikrein-kinin and renin-angiotensin (KKS-RAS) systems represent two highly regulated proteolytic systems that are involved in several physiological and pathological processes. Although their protein-protein interactions can be studied using experimental approaches, it is difficult to differentiate between direc...

journal_title：Biological chemistry

authors： Stoka V,Turk V

更新日期：2010-04-01 00:00:00

abstract：:Among the Ras family, Ran is a unique small G protein. It does not have a lipid modification motif at the C-terminus to bind to the membrane, which is often observed within the Ras family. Ran may therefore interact with a wide range of proteins in various intracellular locations. This means that Ran could play many d...

journal_title：Biological chemistry

authors： Nishimoto T

更新日期：2000-05-01 00:00:00

abstract：:Rhomboids are ubiquitous intramembrane serine proteases that cleave transmembrane substrates. Their functions include growth factor signaling, mitochondrial homeostasis, and parasite invasion. A recent study revealed that the Escherichia coli rhomboid protease EcGlpG is essential for its extraintestinal pathogenic col...

journal_title：Biological chemistry

authors： Arutyunova E,Jiang Z,Yang J,Kulepa AN,Young HS,Verhelst S,O'Donoghue AJ,Lemieux MJ

更新日期：2018-11-27 00:00:00

abstract：:A number of unexpected pathophysiological connections linking different neurodegenerative diseases have emerged over the past decade. An example is provided by prion and Alzheimer's diseases. Despite being distinct pathologies, these disorders share several neurotoxic mechanisms, including accumulation of misfolded pr...

journal_title：Biological chemistry

authors： Elezgarai SR,Biasini E

更新日期：2016-11-01 00:00:00

abstract：:Proteomic identification of protease cleavage site specificity (PICS) is a recent proteomic approach for the easy mapping of protease subsite preferences that determines both the prime- and non-prime side specificity concurrently. Here we greatly facilitate user access by providing an automated and simple web-based da...

journal_title：Biological chemistry

authors： Schilling O,auf dem Keller U,Overall CM

更新日期：2011-11-01 00:00:00

abstract：:Neuritic plaques of Alzheimer patients are composed of multiple protein components. Among them, the amyloid beta-peptides (Abeta) 1-40/42 and further N- and C-terminally modified fragments of Abeta are highly abundant. Most prominent are the isoaspartate (isoAsp)-Abeta peptides and pyroglutamyl (pGlu)-Abeta. While pGl...

journal_title：Biological chemistry

authors： Böhme L,Hoffmann T,Manhart S,Wolf R,Demuth HU

更新日期：2008-08-01 00:00:00

abstract：:The three isoforms of human Ins(1,4,5)P3 3-kinase (IP3K) show remarkable differences in their intracellular targeting. Whereas predominant targeting to the cytoskeleton and endoplasmic reticulum has been shown for IP3K-A and IP3K-B, rat IP3K-C shuttles actively between the nucleus and cytoplasm. In the present study w...

journal_title：Biological chemistry

authors： Nalaskowski MM,Windhorst S,Stockebrand MC,Mayr GW

更新日期：2006-05-01 00:00:00

abstract：:Group II introns are autocatalytic RNAs which self-splice in vitro. However, in vivo additional protein factors might be involved in the splicing process. We used an affinity chromatography method called 'StreptoTag' to identify group II intron binding proteins from Saccharomyces cerevisiae. This method uses a hybrid ...

journal_title：Biological chemistry

authors： Böck-Taferner P,Wank H

更新日期：2004-07-01 00:00:00

abstract：:A large number of prostate cancer (PCa) patients receive treatment without significant benefits, strengthening the need for accurate prognosis, which can be supported by the study of miRNAs. In silico specificity analysis was performed for the identification of miRNAs able to regulate KLK2 and KLK4 expression. Total R...

journal_title：Biological chemistry

authors： Avgeris M,Stravodimos K,Scorilas A

更新日期：2014-09-01 00:00:00

abstract：:Consumption of green tea is associated with a decrease in cardiovascular mortality. The beneficial health effects of green tea are attributed in part to polyphenols, organic compounds found in tea that lower blood pressure, reduce body fat, decrease LDL cholesterol, and inhibit inflammation. We hypothesized that epiga...

journal_title：Biological chemistry

authors： Yamakuchi M,Bao C,Ferlito M,Lowenstein CJ

更新日期：2008-07-01 00:00:00

abstract：:The relationship between the structure and activity of meta- and para-hydroxylated monophenols was studied during their tyrosinase-catalysed hydroxylation and the rate-limiting steps of the reaction mechanism were identified. The para-hydroxylated substrates permit us to study the effect of a substituent (R) in the ca...

journal_title：Biological chemistry

authors： Fenoll LG,Rodríguez-López JN,Varón R,García-Ruiz PA,García-Cánovas F,Tudela J

更新日期：2000-04-01 00:00:00

abstract：:There are enzymatic and non-enzymatic mechanisms that generate NO* from nitrite in blood, stomach, saliva, urine and skin. In blood vessels, nitrite-derived NO* can provide protection via compensatory vasodilation during hypoxia, and in various body fluids it may have antibacterial activity. In the skin, nitrite-deriv...

journal_title：Biological chemistry

authors： Suschek CV,Schewe T,Sies H,Kröncke KD

更新日期：2006-05-01 00:00:00

abstract：:The surface topography of red blood cells (RBCs) was investigated under near-physiological conditions using atomic force microscopy (AFM). An immobilization protocol was established where RBCs are coupled via molecular bonds of the membrane glycoproteins to wheat germ agglutinin (WGA), which is covalently and flexibly...

journal_title：Biological chemistry

authors： Kamruzzahan AS,Kienberger F,Stroh CM,Berg J,Huss R,Ebner A,Zhu R,Rankl C,Gruber HJ,Hinterdorfer P

更新日期：2004-10-01 00:00:00

abstract：:The occurrence of rhodanese-like proteins in the major evolutionary phyla, together with the observed abundance of these proteins also within the same genome, suggests that their function cannot be limited to cyanide scavenging. The aim of the present study was to investigate whether Azotobacter vinelandii RhdA, an en...

journal_title：Biological chemistry

authors： Cereda A,Forlani F,Iametti S,Bernhardt R,Ferranti P,Picariello G,Pagani S,Bonomi F

更新日期：2003-10-01 00:00:00

abstract：:Bone morphogenetic proteins (BMP) are dimeric factors initiating several distinct signaling cascades by binding to two types of transmembrane serine/threonine kinase receptors (BRI and BRII), and are thus regulating several steps in embryonal development and adult tissue homeostasis. BMP-2 contains two symmetrical pai...

journal_title：Biological chemistry

authors： Knaus P,Sebald W

更新日期：2001-08-01 00:00:00

abstract：:The enzymatic properties of glycosylphosphatidylinositol-specific phospholipase D (EC 3.1.4.50) were characterized using a 6,000-fold purified enzyme. This was obtained in 100 microg amounts from human serum with a recovery of 35%. Pure alkaline phosphatase containing one anchor moiety per molecule was used as substra...

journal_title：Biological chemistry

authors： Rhode H,Schulze M,Cumme GA,Göhlert A,Blume E,Bublitz R,Schilling K,Horn A

更新日期：2000-05-01 00:00:00

abstract：:The cysteine-type peptidase cathepsin X is highly upregulated in several cancers and presumably promotes tumor invasion through bypassing cellular senescence. Here, we present first evidence that the underlying mechanism may involve the regulation of the insulin-like growth factor (IGF) system, a well-known activator ...

journal_title：Biological chemistry

authors： Kraus S,Fruth M,Bunsen T,Nägler DK

更新日期：2012-12-01 00:00:00

abstract：:Here, we describe a novel member in the group of membrane-anchored chymotrypsin (S1)-like serine proteases, namely testis serine protease 1 (T-SP1), as it is principally expressed in testis tissue. The human T-SP1 gene encompasses 28.7 kb on the short arm of chromosome 8 and consists of seven exons. Rapid amplificatio...

journal_title：Biological chemistry

authors： Neth P,Profanter B,Geissler C,Nägler DK,Nerlich A,Sommerhoff CP,Jochum M

更新日期：2008-12-01 00:00:00