Efficacy of HOCl scavenging by sulfur-containing compounds: antioxidant activity of glutathione disulfide?

abstract：:Abstract Few genes are known to be involved in renal cell carcinoma (RCC) development and progression. The cell-specific transcription factor hepatocyte nuclear factor 4 alpha (HNF4 alpha) is down-regulated in RCC and we have shown that HNF4 alpha inhibits cell proliferation in the embryonic kidney cell line HEK293. T...

journal_title：Biological chemistry

authors： Grigo K,Wirsing A,Lucas B,Klein-Hitpass L,Ryffel GU

更新日期：2008-02-01 00:00:00

abstract：:Isolated human granulocyte plasma membranes contain progelatinase B. The binding of progelatinase B to the membrane, however, is relatively weak, and a considerable part of progelatinase B can be removed by simply washing the membrane with buffer. This detachment does not depend on the ionic strength of the buffer, in...

journal_title：Biological chemistry

authors： Kolkenbrock H,Zimmermann J,Burmester GR,Ulbrich N

更新日期：2000-01-01 00:00:00

abstract：:MHC encoded DM heterodimers and classical MHC class II complexes meet in an endosomal/lysosomal compartment where DM heterodimers support peptide loading of MHC class II. Studies on peptide loading of rat class II and on peptide persistence in cells of the dendritic lineage prompted us to establish full length cDNA cl...

journal_title：Biological chemistry

authors： Kurth D,Neumann J,Demleitner K,Hildmann J,Mehlig M,Scheicher C,Wendling U,Sun D,Reske K

更新日期：1997-09-01 00:00:00

abstract：:Disulfide bonds play a critical role in a variety of structural and mechanistic processes associated with proteins inside the cells and in the extracellular environment. The thioredoxin family of proteins like thioredoxin (Trx), glutaredoxin (Grx) and protein disulfide isomerase, are involved in the formation, transfe...

journal_title：Biological chemistry

authors： Sousa SF,Neves RPP,Waheed SO,Fernandes PA,Ramos MJ

更新日期：2019-04-24 00:00:00

abstract：:Five moeA mutants were generated by replacing some conserved amino acids of MoeA by site-directed mutagenesis. The mutants were assayed for the ability to restore in vivo nitrate reductase activity of the moeA mutant Escherichia coli JRG97 and in vitro Neurospora crassa nit-1 nitrate reductase activity. The replacemen...

journal_title：Biological chemistry

authors： Sandu C,Brandsch R

更新日期：2002-02-01 00:00:00

abstract：:Lipids serve many distinct functions in cellular homeostasis such as membrane organization, as a platform for membrane function and protein/protein or protein/lipid interaction, energy storage, as well as secondary messengers in signal transduction. Perturbations in lipid homeostasis may result in abnormal cellular fu...

journal_title：Biological chemistry

authors： Xiang Y,Lam SM,Shui G

更新日期：2015-12-01 00:00:00

abstract：:Many aspects of the RNA maturation leave traces in RNA sequencing data in the form of deviations from the reference genomic DNA. This includes, in particular, genomically non-encoded nucleotides and chemical modifications. The latter leave their signatures in the form of mismatches and conspicuous patterns of sequenci...

journal_title：Biological chemistry

authors： Findeiss S,Langenberger D,Stadler PF,Hoffmann S

更新日期：2011-04-01 00:00:00

abstract：:Hsp70 chaperone systems play an essential role in the life cycle of many proteins not only in an hostile environment but also under normal growth conditions. In the course of evolution the diversification of functions was accompanied by an amplification of components of the Hsp70 system. Here strategies are reviewed h...

journal_title：Biological chemistry

authors： Mayer MP,Bukau B

更新日期：1998-03-01 00:00:00

abstract：:Glutaminyl cyclases (QC) catalyze the intramolecular cyclization of N-terminal glutamine residues of peptides and proteins. For a comparison of the substrate specificity of human and papaya QC enzymes, a novel continuous assay was established by adapting an existing discontinuous method. Specificity constants (kcat/Km...

journal_title：Biological chemistry

authors： Schilling S,Manhart S,Hoffmann T,Ludwig HH,Wasternack C,Demuth HU

更新日期：2003-12-01 00:00:00

abstract：:Matrix metalloproteases from the cell surface cleave an 80 kDa E-cadherin fragment (sE-CAD) that induces invasion of cancer cells into collagen type I and inhibits cellular aggregation. Conditioned media from MDCKts.srcCl2 cells at 40 degrees C and 35 degrees C, PCm.src5 and COLO-16 cells at 37 degrees C contained spo...

journal_title：Biological chemistry

authors： Ryniers F,Stove C,Goethals M,Brackenier L,Noë V,Bracke M,Vandekerckhove J,Mareel M,Bruyneel E

更新日期：2002-01-01 00:00:00

abstract：:Five phage displayed peptide libraries were screened for binders to C1q, the recognition subunit of the classical complement pathway. Two rounds of panning resulted in the isolation and characterisation of several different phage displayed C1q-binding peptides from all five libraries. Two groups of the characterised p...

journal_title：Biological chemistry

authors： Lauvrak V,Brekke OH,Ihle O,Lindqvist BH

更新日期：1997-12-01 00:00:00

abstract：:The T-knot scaffold, a disulphide-reinforced structural motif shared by several proteins with very different biological functions, has been defined as 'a stretch of the protein chain which comprises two strands of a beta-sheet and three loops, knotted by two disulphides into the shape of the letter T'. In this communi...

journal_title：Biological chemistry

authors： Polticelli F,Pascarella S,Bordo D,Bolognesi M,Ascenzi P

更新日期：1999-10-01 00:00:00

abstract：:Early diagnostics at the preclinical stage of Alzheimer's disease is of utmost importance for drug development in clinical trials and prognostic guidance. Since soluble Aβ oligomers are considered to play a crucial role in the disease pathogenesis, several methods aim to quantify Aβ oligomers in body fluids such as ce...

journal_title：Biological chemistry

authors： Kravchenko K,Kulawik A,Hülsemann M,Kühbach K,Zafiu C,Herrmann Y,Linnartz C,Peters L,Bujnicki T,Willbold J,Bannach O,Willbold D

更新日期：2017-04-01 00:00:00

abstract：:Most excitatory synaptic transmissions in the central nervous system are mediated by the neurotransmitter glutamate. Binding of glutamate released from the presynaptic membrane causes glutamate receptors in the postsynaptic membrane to open, which results in a transient depolarization of the postsynaptic membrane. The...

journal_title：Biological chemistry

authors： Nakagawa T,Cheng Y,Sheng M,Walz T

更新日期：2006-02-01 00:00:00

abstract：:The amyloid precursor protein (APP) and its processing are widely believed to be central for the etiology of Alzheimer's disease (AD) and appear essential for neuronal development and cell homeostasis in mammals. Many studies show the proteolysis of APP by the proteases α-, β- and γ-secretase, functional aspects of th...

journal_title：Biological chemistry

authors： Coburger I,Hoefgen S,Than ME

更新日期：2014-05-01 00:00:00

abstract：:The p53 tumour suppressor gene is frequently mutated in human tumours and different tumour-derived mutations have varying effects on cells. The effect of a novel tumour-derived p53 mutation and two recently described mutations from South African breast cancer patients on the growth rate, colony formation, cell cycle a...

journal_title：Biological chemistry

authors： Donninger H,Binder A,Bohm L,Parker MI

更新日期：2008-01-01 00:00:00

abstract：:The molecular architectures of the catalytic core of protein phosphatase 1 (PP1) and protein phosphatase 2B (PP2B) are similar, and both contain a beta12-beta13 loop that consists of non-conserved residues. A truncation mutant containing the PP2B catalytic domain has previously been constructed in our laboratory, and ...

journal_title：Biological chemistry

authors： Xie XJ,Xue CZ,Huang W,Yu DY,Wei Q

更新日期：2006-10-01 00:00:00

abstract：:Signalling molecules are produced, degraded, modified and transported throughout the development of higher organisms. Understanding their mode of action implies understanding these dynamics in vivo and in real time. Genetically encoded biosensors are being more and more used as tools to 'follow' signalling molecules a...

journal_title：Biological chemistry

authors： Martin-Arevalillo R,Vernoux T

更新日期：2019-03-26 00:00:00

abstract：:The surface topography of red blood cells (RBCs) was investigated under near-physiological conditions using atomic force microscopy (AFM). An immobilization protocol was established where RBCs are coupled via molecular bonds of the membrane glycoproteins to wheat germ agglutinin (WGA), which is covalently and flexibly...

journal_title：Biological chemistry

authors： Kamruzzahan AS,Kienberger F,Stroh CM,Berg J,Huss R,Ebner A,Zhu R,Rankl C,Gruber HJ,Hinterdorfer P

更新日期：2004-10-01 00:00:00

abstract：:Interleukin-6 (IL-6) is a 185 amino acid residue helical cytokine with various biological activities (e. g. B cell development, acute phase reaction). We have investigated the role of the 168-185 C-terminal region of IL-6 in the induction of fibrinogen synthesis and expression of junB mRNA using synthetic peptides cor...

journal_title：Biological chemistry

authors： Igaz P,Bösze S,Tóth S,Falus A,Hudecz F

更新日期：2001-04-01 00:00:00

abstract：:The enzyme L-asparaginase is a crucial component in the treatment of acute lymphoblastic leukemia (ALL). As all asparaginases in clinical use are derived from microorganisms, immunological reactions are the most important adverse events associated with asparaginase treatment. Two different methods, phage display and t...

journal_title：Biological chemistry

authors： Werner A,Röhm KH,Müller HJ

更新日期：2005-06-01 00:00:00

abstract：:Cellular proteomes are dynamic and adjusted to permanently changing conditions by ATP-fueled proteolytic machineries. Among the five AAA+ proteases in Escherichia coli FtsH is the only essential and membrane-anchored metalloprotease. FtsH is a homohexamer that uses its ATPase domain to unfold and translocate substrate...

journal_title：Biological chemistry

authors： Bittner LM,Arends J,Narberhaus F

更新日期：2017-05-01 00:00:00

abstract：:Arginine deiminase (ADI) is a potential antitumor agent for the arginine deprivation treatment of L-arginine auxotrophic tumors. The optimum pH of ADI varies significantly, yet little is known about the origin of this variety. Here, Pseudomonas aeruginosa ADI (PaADI), an enzyme that functions only at acidic pH, was ut...

journal_title：Biological chemistry

authors： Ding H,Liu H,Yin Y,Ding Y,Jia Y,Chen Q,Zou G,Zheng Z

更新日期：2012-09-01 00:00:00

abstract：:Molecular chaperones are well known as facilitators of protein folding and assembly. However, in recent years multiple chaperone-assisted degradation pathways have also emerged, including CAP (chaperone-assisted proteasomal degradation), CASA (chaperone-assisted selective autophagy), and CMA (chaperone-mediated autoph...

journal_title：Biological chemistry

authors： Kettern N,Dreiseidler M,Tawo R,Höhfeld J

更新日期：2010-05-01 00:00:00

abstract：:In mammalian cells, glucosylceramide (GlcCer), the simplest glycosphingolipid, is hydrolyzed by the lysosomal enzyme acid beta-glucosidase (GlcCerase). In the human metabolic disorder Gaucher disease, GlcCerase activity is significantly decreased owing to one of approximately 200 mutations in the GlcCerase gene. The m...

journal_title：Biological chemistry

authors： Kacher Y,Brumshtein B,Boldin-Adamsky S,Toker L,Shainskaya A,Silman I,Sussman JL,Futerman AH

更新日期：2008-11-01 00:00:00

abstract：:DEAD-box helicases catalyze RNA duplex unwinding in an ATP-dependent reaction. Members of the DEAD-box helicase family consist of a common helicase core formed by two RecA-like domains. According to the current mechanistic model for DEAD-box mediated RNA unwinding, binding of RNA and ATP triggers a conformational chan...

journal_title：Biological chemistry

authors： Rudolph MG,Klostermeier D

更新日期：2015-08-01 00:00:00

abstract：:The cloning of a Ca2+(0)-sensing receptor (CaR) from parathyroid and kidney, coupled with the identification of inherited disorders in Ca2+(0)-sensing resulting from inactivating or activating mutations of this CaR, has provided substantial insights into how Ca2+(0) is regulated at a systemic level. The CaR plays a ce...

journal_title：Biological chemistry

authors： Chattopadhyay N,Vassilev PM,Brown EM

更新日期：1997-08-01 00:00:00

abstract：:The conversion of cellular prion protein (PrP(C)) into its pathological isoform (PrP(Sc)) conveys an increase in hydrophobicity and induces a partial resistance to proteinase K (PK). Interestingly, co-incubation with high copper ion concentrations also modifies the solubility of PrP(c) and induces a partial PK resista...

journal_title：Biological chemistry

authors： Kuczius T,Buschmann A,Zhang W,Karch H,Becker K,Peters G,Groschup MH

更新日期：2004-08-01 00:00:00

abstract：:While the role of C2-ceramide in the induction of programmed cell death (PCD) in animal systems has been well documented, little is known of its role in plant cells. Here we show that C2-ceramide induces PCD in Arabidopsis suspension cultures, which is preceded by the generation of a calcium transient and an increase ...

journal_title：Biological chemistry

authors： Townley HE,McDonald K,Jenkins GI,Knight MR,Leaver CJ

更新日期：2005-02-01 00:00:00

abstract：:We present a method that allows preparing long DNA containing defined mismatches without the use of gel electrophoretic or chromatographic purification steps. The preparation starts with the synthesis of two PCR products, which are identical except for those positions that will later form the mismatches. One of the PC...

journal_title：Biological chemistry

authors： Thomas E,Pingoud A,Friedhoff P

更新日期：2002-09-01 00:00:00