Efficacy of HOCl scavenging by sulfur-containing compounds: antioxidant activity of glutathione disulfide?


:Hypochlorous acid (HOCl) is a bactericidal compound formed by activated neutrophils during inflammation. Overproduction of HOCl causes damage to tissues at the site of neutrophil accumulation. The deleterious effects of excessive HOCl formation can be attenuated using antioxidants. Thiols and thioethers are known to be very effective HOCl scavengers. In the present study, the potency of several sulfur-containing compounds to protect acetylcholinesterase, glutathione S-transferase P1-1 (GST P1-1) and alpha1-antiproteinease against inactivation by HOCl was determined. Surprisingly, glutathione disulfide was an effective protector of acetylcholinesterase against hypochlorous acid. Glutathione disulfide did not provide protection for GST P1-1 and alpha1-antiproteinease against oxidative inactivation by HOCl. The implications of this finding are discussed.


Biol Chem


Biological chemistry


den Hartog GJ,Haenen GR,Vegt E,van der Vijgh WJ,Bast A





Has Abstract


2002-03-01 00:00:00












  • The XPD helicase: XPanDing archaeal XPD structures to get a grip on human DNA repair.

    abstract::Xeroderma pigmentosum complementation group D protein (XPD) is an iron-sulfur cluster containing 5'-3' helicase and, in humans, part of the transcription factor TFIIH. TFIIH is involved in nucleotide excision repair as well as in transcription initiation. Recently, three different groups have reported the structures o...

    journal_title:Biological chemistry

    pub_type: 杂志文章,评审


    authors: Wolski SC,Kuper J,Kisker C

    更新日期:2010-07-01 00:00:00

  • Changes of the peripheral blood mononuclear cells membrane fluidity from type 1 Gaucher disease patients: an electron paramagnetic resonance study.

    abstract::Gaucher disease (GD) is a lysosomal storage disorder, caused by an impaired function of β-glucocerebrosidase, which results in accumulation of glucocerebroside in cells, and altered membrane ordering. Using electron paramagnetic resonance spin labeling, a statistically significant difference in the order parameter bet...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Pavićević A,Lakočević M,Popović M,Popović-Bijelić A,Daković M,Mojović M

    更新日期:2018-04-25 00:00:00

  • Reverse action of ribonuclease T1 in frozen aqueous systems.

    abstract::We have studied ribonuclease T1 (EC 3.1-27.3)-catalysed synthesis of guanylyl-(3'-->5')cytidine in frozen aqueous reaction mixtures at -10 degrees C and in solution at 0 degree C in order to investigate whether ribonuclease-catalysed synthetic reactions can take advantage of the yield-increasing effect of freezing as ...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Haensler M,Hahn U,Jakubke HD

    更新日期:1997-02-01 00:00:00

  • [18O]-labeled singlet oxygen as a tool for mechanistic studies of 8-oxo-7,8-dihydroguanine oxidative damage: detection of spiroiminodihydantoin, imidazolone and oxazolone derivatives.

    abstract::A water-soluble [18O]-labeled endoperoxide derived from N,N'-di(2,3-dihydroxypropyl)-1,4-naphthalene-dipropanamide (DHPN18O2) has been shown to act as a clean chemical source of [18O]-labeled molecular singlet oxygen. This allows the assessment of the singlet oxygen (1O2) reactivity toward biological targets such as D...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Martinez GR,Medeiros MH,Ravanat JL,Cadet J,Di Mascio P

    更新日期:2002-03-01 00:00:00

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    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Xie XJ,Xue CZ,Huang W,Yu DY,Wei Q

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    journal_title:Biological chemistry

    pub_type: 杂志文章,评审


    authors: Boye TL,Nylandsted J

    更新日期:2016-10-01 00:00:00

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    journal_title:Biological chemistry

    pub_type: 杂志文章,评审


    authors: Schulz I,Krause E,González A,Göbel A,Sternfeld L,Schmid A

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  • Genomic expansion across the albumin gene family on human chromosome 4q is directional.

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    pub_type: 杂志文章


    authors: Nishio H,Hamdi HK,Dugaiczyk A

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    authors: Werner A,Röhm KH,Müller HJ

    更新日期:2005-06-01 00:00:00

  • Diversity of GPI-anchored fungal adhesins.

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    journal_title:Biological chemistry

    pub_type: 杂志文章,评审


    authors: Essen LO,Vogt MS,Mösch HU

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    journal_title:Biological chemistry

    pub_type: 杂志文章,评审


    authors: Bui N,Strub K

    更新日期:1999-02-01 00:00:00

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    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Nakagawa T,Cheng Y,Sheng M,Walz T

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    journal_title:Biological chemistry

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    authors: Hofer T,Seo AY,Prudencio M,Leeuwenburgh C

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    journal_title:Biological chemistry



    authors: Kornberg RD

    更新日期:2001-08-01 00:00:00

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    journal_title:Biological chemistry

    pub_type: 杂志文章


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    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Shenai BR,Semenov AV,Rosenthal PJ

    更新日期:2002-05-01 00:00:00

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    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Fischer EH

    更新日期:2010-02-01 00:00:00

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    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Planque C,Aïnciburu M,Heuzé-Vourc'h N,Régina S,de Monte M,Courty Y

    更新日期:2006-06-01 00:00:00

  • Posttranslational modifications and proteinopathies: how guardians of the proteome are defeated.

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    journal_title:Biological chemistry

    pub_type: 杂志文章,评审


    authors: Olzscha H

    更新日期:2019-06-26 00:00:00

  • Tapasin and other chaperones: models of the MHC class I loading complex.

    abstract::MHC (major histocompatibility complex) class I molecules bind intracellular virus-derived peptides in the endoplasmic reticulum (ER) and present them at the cell surface to cytotoxic T lymphocytes. Peptide-free class I molecules at the cell surface, however, could lead to aberrant T cell killing. Therefore, cells ensu...

    journal_title:Biological chemistry

    pub_type: 杂志文章,评审


    authors: Wright CA,Kozik P,Zacharias M,Springer S

    更新日期:2004-09-01 00:00:00

  • Synthetic biology of B cell activation: understanding signal amplification at the B cell antigen receptor using a rebuilding approach.

    abstract::Upon activation of the B cell antigen receptor (BCR), the spleen tyrosine kinase (Syk) and the Src family kinase Lyn phosphorylate tyrosines of the immunoreceptor tyrosine-based activation motif (ITAM) of Igα and Igβ which further serve as binding sites for the SH2 domains of these kinases. Using a synthetic biology a...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Kulathu Y,Zuern C,Yang J,Reth M

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  • Binding and activation of the human plasma kinin-forming system on the cell walls of Candida albicans and Candida tropicalis.

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    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Karkowska-Kuleta J,Kozik A,Rapala-Kozik M

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    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Böhm B,Heinzelmann S,Motz M,Bauer G

    更新日期:2015-12-01 00:00:00

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    abstract::Recombinant expression of actin in bacteria results in non-native species that aggregate into inclusion bodies. Actin is a folding substrate of TRiC, the chaperonin of the eukaryotic cytosol. By employing bacterial in vitro translation lysates supplemented with purified chaperones, we have found that TRiC is the only ...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Stemp MJ,Guha S,Hartl FU,Barral JM

    更新日期:2005-08-01 00:00:00

  • Monitoring human parvovirus B19 virus-like particles and antibody complexes in solution by fluorescence correlation spectroscopy.

    abstract::Fluorescence correlation spectroscopy (FCS) was used in monitoring human parvovirus B19 virus-like particle (VLP) antibody complexes from acute phase and past-immunity serum samples. The Oregon Green 488-labeled VLPs gave an average diffusion coefficient of 1.7 x 10(-7) cm2 s(-1) with an apparent hydrodynamic radius o...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Toivola J,Michel PO,Gilbert L,Lahtinen T,Marjomäki V,Hedman K,Vuento M,Oker-Blom C

    更新日期:2004-01-01 00:00:00

  • New glycyl radical enzymes catalysing key metabolic steps in anaerobic bacteria.

    abstract::During the last decade, an increasing number of new enzymes containing glycyl radicals in their active sites have been identified and biochemically characterised. These include benzylsuccinate synthase (Bss), 4-hydroxyphenylacetate decarboxylase (Hpd) and the coenzyme B12-independent glycerol dehydratase (Gdh). These ...

    journal_title:Biological chemistry

    pub_type: 杂志文章,评审


    authors: Selmer T,Pierik AJ,Heider J

    更新日期:2005-10-01 00:00:00

  • The DEAH helicase DHX36 and its role in G-quadruplex-dependent processes.

    abstract::DHX36 is a member of the DExD/H box helicase family, which comprises a large number of proteins involved in various cellular functions. Recently, the function of DHX36 in the regulation of G-quadruplexes (G4s) was demonstrated. G4s are alternative nucleic acid structures, which influence many cellular pathways on a tr...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Schult P,Paeschke K

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  • 'Dipeptidyl peptidase-IV activity and/or structure homologs' (DASH) in growth-modulated glioma cell lines.

    abstract::Dipeptidyl peptidase-IV has been demonstrated to play a role in cancer biology by many authors. Since then, additional proteins possessing similar enzymatic activity have been described and their role in cancerogenesis has been hypothesized. To assess the complexity of these 'Dipeptidyl peptidase-IV activity and/or st...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Sedo A,Busek P,Scholzová E,Malík R,Vlasicová K,Janácková S,Mares V

    更新日期:2004-06-01 00:00:00

  • Synthetic peptides and fluorogenic substrates related to the reactive site sequence of Kunitz-type inhibitors isolated from Bauhinia: interaction with human plasma kallikrein.

    abstract::We have previously described Kunitz-type serine proteinase inhibitors purified from Bauhinia seeds. Human plasma kallikrein shows different susceptibility to those inhibitors. In this communication, we describe the interaction of human plasma kallikrein with fluorogenic and non-fluorogenic peptides based on the Bauhin...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Oliva ML,Santomauro-Vaz EM,Andrade SA,Juliano MA,Pott VJ,Sampaio MU,Sampaio CA

    更新日期:2001-01-01 00:00:00

  • Structure and function of the human parvulins Pin1 and Par14/17.

    abstract::Parvulins belong to the family of peptidyl-prolyl cis/trans isomerases (PPIases) assisting in protein folding and in regulating the function of a broad variety of proteins in all branches of life. The human representatives Pin1 and Par14/17 are directly involved in processes influencing cellular maintenance and cell f...

    journal_title:Biological chemistry

    pub_type: 杂志文章,评审


    authors: Matena A,Rehic E,Hönig D,Kamba B,Bayer P

    更新日期:2018-01-26 00:00:00