Novel approach to quorum quenching: rational design of antibacterials in combination with hexahistidine-tagged organophosphorus hydrolase.

Abstract:

:N-acyl homoserine lactones (AHLs) are quorum sensing (QS) signal molecules used by most Gram-negative pathogenic bacteria. In this article the lactonase activity of the preparations based on hexahistidine-tagged organophosphorus hydrolase (His6-OPH) towards AHLs was studied. Initially, three of the most interesting β-lactam antibiotics were selected from seven that were trialed during molecular docking to His6-OPH. Combinations of antibiotics (meropenem, imipenem, ceftriaxone) and His6-OPH taken in the native form or in the form of non-covalent enzyme-polyelectrolyte complexes (EPCs) with poly(glutamic acid) or poly(aspartic acid) were obtained and investigated. The lactonase activity of the preparations was investigated under different physical-chemical conditions in the hydrolysis of AHLs [N-butyryl-D,L-homoserine lactone, N-(3-oxooctanoyl)-D,L-homoserine lactone, N-(3-oxododecanoyl)-L-homoserine lactone]. An increased efficiency of catalytic action and stability of the lactonase activity of His6-OPH was shown for its complexes with antibiotics and was confirmed in trials with bacterial strains. The broadening of the catalytic action of the enzyme against AHLs was revealed in the presence of the meropenem. Results of molecular docking of AHLs to the surface of the His6-OPH dimer in the presence of antibiotics allowed proposing the mechanism of such interference based on a steric repulsion of the carbon chain of hydrolyzed AHLs by the antibiotics bounded to the enzyme surface.

journal_name

Biol Chem

journal_title

Biological chemistry

authors

Aslanli A,Lyagin I,Efremenko E

doi

10.1515/hsz-2018-0162

subject

Has Abstract

pub_date

2018-07-26 00:00:00

pages

869-879

issue

8

eissn

1431-6730

issn

1437-4315

pii

/j/bchm.just-accepted/hsz-2018-0162/hsz-2018-0162.

journal_volume

399

pub_type

杂志文章
  • Importance of tRNA interactions with 23S rRNA for peptide bond formation on the ribosome: studies with substrate analogs.

    abstract::The major enzymatic activity of the ribosome is the catalysis of peptide bond formation. The active site -- the peptidyl transferase center -- is composed of ribosomal RNA (rRNA), and interactions between rRNA and the reactants, peptidyl-tRNA and aminoacyl-tRNA, are crucial for the reaction to proceed rapidly and effi...

    journal_title:Biological chemistry

    pub_type: 杂志文章,评审

    doi:10.1515/BC.2007.077

    authors: Beringer M,Rodnina MV

    更新日期:2007-07-01 00:00:00

  • ACE inhibition enhances bradykinin relaxations through nitric oxide and B1 receptor activation in bovine coronary arteries.

    abstract::Bradykinin causes vascular relaxations through release of endothelial relaxing factors including prostacyclin, nitric oxide (NO) and epoxyeicosatrienoic acids (EETs). Bradykinin is metabolized by angiotensin converting enzyme (ACE) and ACE inhibition enhances bradykinin relaxations. Our goal was to characterize the ro...

    journal_title:Biological chemistry

    pub_type: 杂志文章

    doi:10.1515/hsz-2012-0348

    authors: Gauthier KM,Cepura CJ,Campbell WB

    更新日期:2013-09-01 00:00:00

  • Kallikrein 6 is a mediator of K-RAS-dependent migration of colon carcinoma cells.

    abstract::Kallikrein 6 (KLK6) is a trypsin-like serine peptidase whose relevance in various types of cancers is currently being explored. Previous studies have shown that KLK6 mRNA is upregulated in colon and gastric cancers; however, the regulatory mechanisms and phenotypic consequences of this upregulation are largely unknown...

    journal_title:Biological chemistry

    pub_type: 杂志文章

    doi:10.1515/BC.2008.087

    authors: Henkhaus RS,Gerner EW,Ignatenko NA

    更新日期:2008-06-01 00:00:00

  • On the regulative role of the glutamate receptor in mitochondria.

    abstract::The purpose of this work was to study the regulative role of the glutamate receptor found earlier in the brain mitochondria. In the present work a glutamate-dependent signaling system with similar features was detected in mitochondria of the heart. The glutamate-dependent signaling system in the heart mitochondria was...

    journal_title:Biological chemistry

    pub_type: 杂志文章

    doi:10.1515/hsz-2015-0289

    authors: Selin AA,Lobysheva NV,Nesterov SV,Skorobogatova YA,Byvshev IM,Pavlik LL,Mikheeva IB,Moshkov DA,Yaguzhinsky LS,Nartsissov YR

    更新日期:2016-05-01 00:00:00

  • Imaging morphological details and pathological differences of red blood cells using tapping-mode AFM.

    abstract::The surface topography of red blood cells (RBCs) was investigated under near-physiological conditions using atomic force microscopy (AFM). An immobilization protocol was established where RBCs are coupled via molecular bonds of the membrane glycoproteins to wheat germ agglutinin (WGA), which is covalently and flexibly...

    journal_title:Biological chemistry

    pub_type: 杂志文章

    doi:10.1515/BC.2004.124

    authors: Kamruzzahan AS,Kienberger F,Stroh CM,Berg J,Huss R,Ebner A,Zhu R,Rankl C,Gruber HJ,Hinterdorfer P

    更新日期:2004-10-01 00:00:00

  • A role for the metalloprotease invadolysin in insulin signaling and adipogenesis.

    abstract::Invadolysin is a novel metalloprotease conserved amongst metazoans that is essential for life in Drosophila. We previously showed that invadolysin was essential for the cell cycle and cell migration, linking to metabolism through a role in lipid storage and interaction with mitochondrial proteins. In this study we dem...

    journal_title:Biological chemistry

    pub_type: 杂志文章

    doi:10.1515/hsz-2016-0226

    authors: Chang CW,Abhinav K,Di Cara F,Panagakou I,Vass S,Heck MM

    更新日期:2017-03-01 00:00:00

  • Epigallocatechin gallate inhibits endothelial exocytosis.

    abstract::Consumption of green tea is associated with a decrease in cardiovascular mortality. The beneficial health effects of green tea are attributed in part to polyphenols, organic compounds found in tea that lower blood pressure, reduce body fat, decrease LDL cholesterol, and inhibit inflammation. We hypothesized that epiga...

    journal_title:Biological chemistry

    pub_type: 杂志文章

    doi:10.1515/BC.2008.095

    authors: Yamakuchi M,Bao C,Ferlito M,Lowenstein CJ

    更新日期:2008-07-01 00:00:00

  • Annexins - insights from knockout mice.

    abstract::Annexins are a highly conserved protein family that bind to phospholipids in a calcium (Ca2+) - dependent manner. Studies with purified annexins, as well as overexpression and knockdown approaches identified multiple functions predominantly linked to their dynamic and reversible membrane binding behavior. However, mos...

    journal_title:Biological chemistry

    pub_type: 杂志文章,评审

    doi:10.1515/hsz-2016-0168

    authors: Grewal T,Wason SJ,Enrich C,Rentero C

    更新日期:2016-10-01 00:00:00

  • Glycine-assisted enhancement of 1,4-beta-d-xylan xylanohydrolase activity at alkaline pH with a pH optimum shift.

    abstract::This is the first report describing the enhancement of xylanase activity by the neutral amino acid glycine. Xylanase activity is increased seven-fold at alkaline pH in the presence of glycine and its pH optimum is shifted from pH 7 to 8 without using any protein engineering techniques. Analysis of the steady-state kin...

    journal_title:Biological chemistry

    pub_type: 杂志文章

    doi:10.1515/BC.2007.007

    authors: Vathipadiekal V,Verma A,Rao M

    更新日期:2007-01-01 00:00:00

  • Pressure- and temperature-induced unfolding studies: thermodynamics of core hydrophobicity and packing of ribonuclease A.

    abstract::In this work we demonstrate that heat and pressure induce only slightly different energetic changes in the unfolded state of RNase A. Using pressure and temperature as denaturants on a significant number of variants, and by determining the free energy of unfolding at different temperatures, we estimated the stability ...

    journal_title:Biological chemistry

    pub_type: 杂志文章

    doi:10.1515/BC.2006.038

    authors: Font J,Benito A,Torrent J,Lange R,Ribó M,Vilanova M

    更新日期:2006-03-01 00:00:00

  • Diversity of GPI-anchored fungal adhesins.

    abstract::Selective adhesion of fungal cells to one another and to foreign surfaces is fundamental for the development of multicellular growth forms and the successful colonization of substrates and host organisms. Accordingly, fungi possess diverse cell wall-associated adhesins, mostly large glycoproteins, which present N-term...

    journal_title:Biological chemistry

    pub_type: 杂志文章,评审

    doi:10.1515/hsz-2020-0199

    authors: Essen LO,Vogt MS,Mösch HU

    更新日期:2020-11-26 00:00:00

  • Small-angle X-ray and neutron scattering as a tool for structural systems biology.

    abstract::Small-angle scattering (SAS) of X-rays and neutrons reveals low-resolution structures of biological macromolecules in solution. With the recent experimental and methodological advances, SAS became a unique tool for characterising biological systems. The method covers an extremely broad range of molecule sizes (from a ...

    journal_title:Biological chemistry

    pub_type: 杂志文章,评审

    doi:10.1515/BC.2010.093

    authors: Svergun DI

    更新日期:2010-07-01 00:00:00

  • Multicellular and unicellular responses of microbial biofilms to stress.

    abstract::Biofilms are a ubiquitous mode of microbial life and display an increased tolerance to different stresses. Inside biofilms, cells may experience both externally applied stresses and internal stresses that emerge as a result of growth in spatially structured communities. In this review, we discuss the spatial scales of...

    journal_title:Biological chemistry

    pub_type: 杂志文章,评审

    doi:10.1515/hsz-2020-0213

    authors: Rode DKH,Singh PK,Drescher K

    更新日期:2020-11-26 00:00:00

  • Cellular prion protein acquires resistance to proteolytic degradation following copper ion binding.

    abstract::The conversion of cellular prion protein (PrP(C)) into its pathological isoform (PrP(Sc)) conveys an increase in hydrophobicity and induces a partial resistance to proteinase K (PK). Interestingly, co-incubation with high copper ion concentrations also modifies the solubility of PrP(c) and induces a partial PK resista...

    journal_title:Biological chemistry

    pub_type: 杂志文章

    doi:10.1515/BC.2004.090

    authors: Kuczius T,Buschmann A,Zhang W,Karch H,Becker K,Peters G,Groschup MH

    更新日期:2004-08-01 00:00:00

  • Functional mapping of the EcoRV DNA methyltransferase by random mutagenesis and screening for catalytically inactive mutants.

    abstract::M.EcoRV is an alpha-adenine DNA methyltransferase. According to structure predictions, the enzyme consists of a catalytic domain, which has a structure similar to all other DNA-methyltransferases, and a smaller DNA-recognition domain. We have investigated this enzyme by random mutagenesis, using error-prone PCR, follo...

    journal_title:Biological chemistry

    pub_type: 杂志文章

    doi:10.1515/bchm.1998.379.4-5.475

    authors: Friedrich T,Roth M,Helm-Kruse S,Jeltsch A

    更新日期:1998-04-01 00:00:00

  • In vitro conversion and seeded fibrillization of posttranslationally modified prion protein.

    abstract::The conversion of the cellular isoform of the prion protein (PrP(C)) into the pathologic isoform (PrP(Sc)) is the key event in prion diseases. To study the conversion process, an in vitro system based on varying the concentration of low amounts of sodium dodecyl sulfate (SDS) has been employed. In the present study, t...

    journal_title:Biological chemistry

    pub_type: 杂志文章

    doi:10.1515/BC.2011.048

    authors: Stöhr J,Elfrink K,Weinmann N,Wille H,Willbold D,Birkmann E,Riesner D

    更新日期:2011-05-01 00:00:00

  • MAP3K1 functionally interacts with Axin1 in the canonical Wnt signalling pathway.

    abstract::A central point of regulation in the Wnt/beta-catenin signalling pathway is the formation of the beta-catenin destruction complex. Axin1, an essential negative regulator of Wnt signalling, serves as a scaffold within this complex and is critical for rapid turnover of beta-catenin. To examine the mechanism by which Wnt...

    journal_title:Biological chemistry

    pub_type: 杂志文章

    doi:10.1515/BC.2010.028

    authors: Sue Ng S,Mahmoudi T,Li VS,Hatzis P,Boersema PJ,Mohammed S,Heck AJ,Clevers H

    更新日期:2010-02-01 00:00:00

  • Evidence for a protective role of the CX3CL1/CX3CR1 axis in a model of amyotrophic lateral sclerosis.

    abstract::Aberrant microglial activation and neuroinflammation is a pathological hallmark of amyotrophic lateral sclerosis (ALS). Fractalkine (CX3CL1) is mostly expressed on neuronal cells. The fractalkine receptor (CX3CR1) is predominantly expressed on microglia. Many progressive neuroinflammatory disorders show disruption of ...

    journal_title:Biological chemistry

    pub_type: 杂志文章

    doi:10.1515/hsz-2018-0204

    authors: Liu C,Hong K,Chen H,Niu Y,Duan W,Liu Y,Ji Y,Deng B,Li Y,Li Z,Wen D,Li C

    更新日期:2019-04-24 00:00:00

  • Nitrite, a naturally occurring precursor of nitric oxide that acts like a 'prodrug'.

    abstract::There are enzymatic and non-enzymatic mechanisms that generate NO* from nitrite in blood, stomach, saliva, urine and skin. In blood vessels, nitrite-derived NO* can provide protection via compensatory vasodilation during hypoxia, and in various body fluids it may have antibacterial activity. In the skin, nitrite-deriv...

    journal_title:Biological chemistry

    pub_type: 杂志文章,评审

    doi:10.1515/BC.2006.065

    authors: Suschek CV,Schewe T,Sies H,Kröncke KD

    更新日期:2006-05-01 00:00:00

  • Exploratory cell dynamics: a sense of touch for cells?

    abstract::Cells need to process multifaceted external cues to steer their dynamic behavior. To efficiently perform this task, cells implement several exploratory mechanisms to actively sample their environment. In particular, cells can use exploratory actin-based cell protrusions and contractions to engage and squeeze the envir...

    journal_title:Biological chemistry

    pub_type: 杂志文章,评审

    doi:10.1515/hsz-2017-0341

    authors: Nalbant P,Dehmelt L

    更新日期:2018-07-26 00:00:00

  • Cell traces--footprints of individual cells during locomotion and adhesion.

    abstract::Animal cells release traces of material onto glass or silicon surfaces during adhesion and migration. This little studied phenomenon is a widespread and normal concomitant of cell migration. The paper introduces the study of such material. The traces can be visualised by different microscopic techniques (e.g. TIRF, IR...

    journal_title:Biological chemistry

    pub_type: 杂志文章

    doi:10.1515/bchm.1998.379.8-9.1161

    authors: Fuhr G,Richter E,Zimmermann H,Hitzler H,Niehus H,Hagedorn R

    更新日期:1998-08-01 00:00:00

  • Oxygen(es) and the hypoxia-inducible factor-1.

    abstract::The hypoxia-inducible factor-1 (HIF-1) is a basic-helix-loop-helix (bHLH) heterodimeric transcription factor activated by reductions in oxygen concentration (hypoxia). Activated HIF-1 upregulates expression of genes involved in the adaptation of higher organisms to hypoxic conditions, caused by e.g. high altitude, ane...

    journal_title:Biological chemistry

    pub_type: 杂志文章,评审

    doi:

    authors: Wenger RH,Gassmann M

    更新日期:1997-07-01 00:00:00

  • Benefits of stable isotope labeling in RNA analysis.

    abstract::RNAs are key players in life as they connect the genetic code (DNA) with all cellular processes dominated by proteins. They contain a variety of chemical modifications and many RNAs fold into complex structures. Here, we review recent progress in the analysis of RNA modification and structure on the basis of stable is...

    journal_title:Biological chemistry

    pub_type: 杂志文章,评审

    doi:10.1515/hsz-2018-0447

    authors: Asadi-Atoi P,Barraud P,Tisne C,Kellner S

    更新日期:2019-06-26 00:00:00

  • Physiology and pathophysiology of the RANKL/RANK system.

    abstract::The TNF family molecule RANKL and its receptor RANK are key regulators of bone remodeling, lymph node formation, and mammary gland development during pregnancy. RANKL and RANK are also expressed in the central nervous systems (CNS). However, the functional relevance of RANKL/RANK in the brain was entirely unknown. Rec...

    journal_title:Biological chemistry

    pub_type: 杂志文章,评审

    doi:10.1515/BC.2010.149

    authors: Hanada R,Hanada T,Penninger JM

    更新日期:2010-12-01 00:00:00

  • A nucleosome-free dG-dC-rich sequence element promotes constitutive transcription of the essential yeast RIO1 gene.

    abstract::RIO1 is an essential gene that encodes a protein serine kinase and is transcribed constitutively at a very low level. Transcriptional activation of RIO1 dispenses with a canonical TATA box as well as with classical transactivators or specific DNA-binding factors. Instead, a dG-dC-rich sequence element, that is located...

    journal_title:Biological chemistry

    pub_type: 杂志文章

    doi:10.1515/BC.2003.143

    authors: Angermayr M,Schwerdffeger K,Bandlow W

    更新日期:2003-09-01 00:00:00

  • Visualisation of transforming growth factor-beta 1, tissue kallikrein, and kinin and transforming growth factor-beta receptors on human clear-cell renal carcinoma cells.

    abstract::Transforming growth factor-beta1 (TGF-beta1) has a biphasic effect on the growth of renal epithelial cells. In transformed cells, TGF-beta1 appears to accelerate the proliferation of malignant cells. The diverse cellular functions of TGF-beta1 are regulated by three high-affinity serine/threonine kinase receptors, nam...

    journal_title:Biological chemistry

    pub_type: 杂志文章

    doi:10.1515/BC.2005.045

    authors: Moodley R,Snyman C,Odhav B,Bhoola KD

    更新日期:2005-04-01 00:00:00

  • Full length cDNA of rat RT1.DMa and RT1.DMb and expression of RT1.DM genes in dendritic and Langerhans cells.

    abstract::MHC encoded DM heterodimers and classical MHC class II complexes meet in an endosomal/lysosomal compartment where DM heterodimers support peptide loading of MHC class II. Studies on peptide loading of rat class II and on peptide persistence in cells of the dendritic lineage prompted us to establish full length cDNA cl...

    journal_title:Biological chemistry

    pub_type: 杂志文章

    doi:10.1515/bchm.1997.378.9.1005

    authors: Kurth D,Neumann J,Demleitner K,Hildmann J,Mehlig M,Scheicher C,Wendling U,Sun D,Reske K

    更新日期:1997-09-01 00:00:00

  • Characterization of the nuclear import mechanisms of HSV-1 UL31.

    abstract::As an important protein, UL31 has been demonstrated to play multiple roles in herpes simplex virus 1 (HSV-1) replication. Previous studies showed that UL31 predominantly locates in the nucleus in chemical fixed cells and live cells, however, the determining mechanisms for its nuclear translocation is not clear. In the...

    journal_title:Biological chemistry

    pub_type: 杂志文章

    doi:10.1515/hsz-2015-0299

    authors: Cai M,Si J,Li X,Zeng Z,Li M

    更新日期:2016-06-01 00:00:00

  • EGFR and FGFR signaling through FRS2 is subject to negative feedback control by ERK1/2.

    abstract::Fibroblast growth factor (FGF) receptor substrate 2 (FRS2) is a membrane-anchored docking protein that has been shown to play an important role in linking FGF, nerve growth factor (NGF) and glial cell-derived neurotrophic factor (GDNF) receptors with the Ras/mitogen-activated protein (MAP) kinase signaling cascade. He...

    journal_title:Biological chemistry

    pub_type: 杂志文章

    doi:10.1515/BC.2003.134

    authors: Wu Y,Chen Z,Ullrich A

    更新日期:2003-08-01 00:00:00

  • Expression of the human kallikrein genes 10 (KLK10) and 11 (KLK11) in cancerous and non-cancerous lung tissues.

    abstract::Only one transcript for KLK10 was identified by RT-PCR in lung tissue, whereas KLK11 expressed at least four alternative transcripts. Quantitative analysis of KLK10 and KLK11 expression levels was assessed by real-time PCR, in a cohort of 47 patients with non-small-cell lung cancer (NSCLC). Expression levels of these ...

    journal_title:Biological chemistry

    pub_type: 杂志文章

    doi:10.1515/BC.2006.098

    authors: Planque C,Aïnciburu M,Heuzé-Vourc'h N,Régina S,de Monte M,Courty Y

    更新日期:2006-06-01 00:00:00