Abstract：
:Interleukin-1 signaling is initiated by recruitment of adapter proteins and kinases to the type I interleukin-1 receptor (IL-1RI). It is modulated by accompanying redox processes at various levels, such as (auto-) phosphorylation of the IL-1RI-associated kinase IRAK, the phosphorylation of IkappaB and translocation and transcriptional activity of NF-kappaB. Here we demonstrate that the thiol-modifying agents diamide, menadione, and phenylarsine oxide (PAO) block the recruitment of IRAK to the receptor without inhibiting kinase activity in the immunoprecipitated IL-1RI complex in the human epithelial cell line ECV304 and the murine T cell line EL-4. Inhibition of IRAK receptor association by menadione is reversible in a GSH-dependent manner, while the PAO effect proved to be irreversible. Phospholipid hydroperoxide glutathione peroxidase attenuates inhibition by menadione. Recruitment correlates with the presence of thiol groups in IRAK that were available for IAIT-labeling. We conclude that recruitment of IRAK to the IL-1RI is redox regulated by the glutathione system, a reduced status being a prerequisite for an appropiate IL-1 response.
journal_name
Biol Chemjournal_title
Biological chemistryauthors
Böl GF,Jurrmann N,Brigelius-Flohé Rdoi
10.1515/BC.2003.068keywords:
subject
Has Abstractpub_date
2003-04-01 00:00:00pages
609-17issue
4eissn
1431-6730issn
1437-4315journal_volume
384pub_type
杂志文章abstract：:A putative operon of the ribosomal proteins S20 and Thx has been determined in a 1.4 kb sequenced region of T. thermophilus genomic DNA. Both genes have a promoter sequence 29 nt upstream of ORF1, possess their own Shine-Dalgarno motifs (GGAG) and are separated by only 9 nucleotides, a feature characteristic of the co...
journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/BC.2001.126
更新日期：2001-07-01 00:00:00
abstract：:Synthetic biologists aim at engineering controllable biological parts such as DNA, RNA and proteins in order to steer biological activities using external inputs. Proteins can be controlled in several ways, for instance by regulating the expression of their encoding genes with small molecules or light. However, post-t...
journal_title：Biological chemistry
pub_type： 杂志文章,评审
doi：10.1515/hsz-2018-0309
更新日期：2019-03-26 00:00:00
abstract：:The activation of progelatinase A to gelatinase A requires cleavage of an asparaginyl bond to form the N-terminus of the mature enzyme. We have asked whether the activation can be mediated by legumain, the recently discovered lysosomal cysteine proteinase that is specific for hydrolysis of asparaginyl bonds. Addition ...
journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/BC.2001.093
更新日期：2001-05-01 00:00:00
abstract：:The cysteine-type peptidase cathepsin X is highly upregulated in several cancers and presumably promotes tumor invasion through bypassing cellular senescence. Here, we present first evidence that the underlying mechanism may involve the regulation of the insulin-like growth factor (IGF) system, a well-known activator ...
journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/hsz-2012-0209
更新日期：2012-12-01 00:00:00
abstract：:Historically considered as accessory cells to neurons, there is an increasing interest in the role of astrocytes in normal and pathological conditions. Astrocytes are involved in neurotransmitter recycling, antioxidant supply, ion buffering and neuroinflammation, i.e. a lot of the same pathways that go astray in Alzhe...
journal_title：Biological chemistry
pub_type： 杂志文章,评审
doi：10.1515/hsz-2019-0140
更新日期：2019-08-27 00:00:00
abstract：:Oncogenic transformation is dependent on activated membrane-associated NADPH oxidase (NOX). However, the resultant extracellular superoxide anions are also driving the NO/peroxynitrite and the HOCl pathway, which eliminates NOX-expressing transformed cells through selective apoptosis induction. Tumor progression is de...
journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/hsz-2014-0234
更新日期：2015-12-01 00:00:00
abstract：:One of the control mechanisms of cathepsin B biosynthesis and trafficking operates through alternative splicing of pre-mRNA. An mRNA lacking exon 2 is more efficiently translated than that containing all exons, and may be responsible for elevated biosynthesis and enzyme routing to the extracellular space, with critica...
journal_title：Biological chemistry
pub_type： 杂志文章,评审
doi：10.1515/BC.2006.125
更新日期：2006-08-01 00:00:00
abstract：:Full-length Hsp70 protein (Hsp70) and the C-terminal domain of Hsp70 (Hsp70C) both stimulate the cytolytic activity of naive natural killer (NK) cells against Hsp70-positive tumor target cells. Here, we describe the characterization of Hsp70-NK cell interaction with binding studies using the human NK cell line YT. Bin...
journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/BC.2003.030
更新日期：2003-02-01 00:00:00
abstract：:Human kallikrein-related peptidases (KLKs), including the well-known prostate cancer biomarker, prostate-specific antigen (PSA-KLK3), along with 14 other serine proteinase KLK family members are now known to regulate cells by cleaving and activating members of the G-protein-coupled proteinase-activated receptor (PAR) ...
journal_title：Biological chemistry
pub_type： 杂志文章,评审
doi：10.1515/hsz-2014-0123
更新日期：2014-09-01 00:00:00
abstract：:Molecular chaperones of the heat shock protein 70 (Hsp70) family play a crucial role in the presentation of exogenous antigenic peptides by antigen-presenting cells (APCs). In a combined biochemical and immunological approach, we characterize the biochemical interaction of tumor-associated peptides with human Hsp70 an...
journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/BC.2009.038
更新日期：2009-04-01 00:00:00
abstract：:Understanding the molecular mechanisms behind extreme temperature stability is of relevance for the protein folding problem and for designing proteins for industrial and medical applications. A powerful approach for understanding the structural basis of thermostability is the comparison of high resolution structures o...
journal_title：Biological chemistry
pub_type： 杂志文章
doi：
更新日期：1997-03-01 00:00:00
abstract：:This article presents a historical account of the discovery of reversible protein phosphorylation. The process was uncovered in the mid-1950s in a study designed to elucidate the complex hormonal regulation of skeletal muscle glycogen phosphorylase. This enzyme was originally thought to be regulated by AMP now known t...
journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/BC.2010.011
更新日期：2010-02-01 00:00:00
abstract：:In order to enhance and target gene delivery we have previously established a novel method, termed magnetofection, which uses magnetic force acting on gene vectors that are associated with magnetic particles. Here we review the benefits, the mechanism and the potential of the method with regard to overcoming physical ...
journal_title：Biological chemistry
pub_type： 杂志文章,评审
doi：10.1515/BC.2003.082
更新日期：2003-05-01 00:00:00
abstract：:Signalling molecules are produced, degraded, modified and transported throughout the development of higher organisms. Understanding their mode of action implies understanding these dynamics in vivo and in real time. Genetically encoded biosensors are being more and more used as tools to 'follow' signalling molecules a...
journal_title：Biological chemistry
pub_type： 杂志文章,评审
doi：10.1515/hsz-2018-0310
更新日期：2019-03-26 00:00:00
abstract：:The three-dimensional structure of pterin-4a-carbinolamine dehydratase (PCD) from Pseudomonas aeruginosa has been solved. Based on this we have investigated the roles of putative active center residues through functional replacement by site-directed mutagenesis. Three histidines, His73, His74 and His91, appear to be i...
journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/bchm.1998.379.12.1427
更新日期：1998-12-01 00:00:00
abstract：:Cathepsin E (CE) is an intracellular aspartic proteinase implicated in various physiological and pathological processes, yet its actual roles in vivo remain elusive. To assess the physiological significance of CE expression in tumor cells, human CE was stably expressed in human prostate carcinoma ALVA101 cells express...
journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/BC.2007.154
更新日期：2007-11-01 00:00:00
abstract：:[NiFe] hydrogenases catalyze reversible hydrogen production/consumption. The active site of [NiFe] hydrogenases contains a complex NiFe(CN)2CO center, and the biosynthesis/maturation of these enzymes is a complex and dynamic process, primarily involving six Hyp proteins (HypABCDEF). HypA and HypB are involved in the N...
journal_title：Biological chemistry
pub_type： 杂志文章,评审
doi：10.1515/hsz-2012-0197
更新日期：2012-09-08 00:00:00
abstract：:Type I allergy, a hypersensitivity disease affecting almost 20% of the population worldwide, is based on the IgE recognition of otherwise harmless antigens (i.e., allergens). Allergen-induced crosslink of effector cell-bound IgE antibodies leads to the release of biological mediators and thus to immediate disease symp...
journal_title：Biological chemistry
pub_type： 杂志文章,评审
doi：10.1515/BC.1999.101
更新日期：1999-07-01 00:00:00
abstract：:Chitinase-like proteins (CLPs) are lectins combining properties of cytokines and growth factors. Human CLPs include YKL-40, YKL-39 and SI-CLP that are secreted by cancer cells, macrophages, neutrophils, synoviocytes, chondrocytes and other cells. The best investigated CLP in cancer is YKL-40. Serum and plasma levels o...
journal_title：Biological chemistry
pub_type： 杂志文章,评审
doi：10.1515/hsz-2015-0269
更新日期：2016-03-01 00:00:00
abstract：:The immunoproteasome subunit low molecular weight protein 2 (LMP2) codon 60 polymorphism has been associated with autoimmune diseases. It has also been demonstrated to influence susceptibility to TNF-alpha-induced apoptosis in blood cells and proteasome activity in aged human brain. In the present study, an in silico ...
journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/BC.2006.056
更新日期：2006-04-01 00:00:00
abstract：:Cysteine proteases of the malaria parasite Plasmodium falciparum, known as falcipains, are promising targets for antimalarial chemotherapy. We evaluated cultured parasites for the stage-specific expression of cysteine proteases and sensitivity to cysteine protease inhibitors. Protease activity and inhibitor sensitivit...
journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/BC.2002.089
更新日期：2002-05-01 00:00:00
abstract：:Two new fluorescent labels are presented that are optimized for excitation with He/Ne laser and red diode lasers. Application in FCS and labeling of proteins and oligomers are demonstrated. A strong rise of quantum yield and emission life time upon binding to biomolecules are characteristic features of the dyes. ...
journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/BC.2001.062
更新日期：2001-03-01 00:00:00
abstract：:The relationship between the structure and activity of meta- and para-hydroxylated monophenols was studied during their tyrosinase-catalysed hydroxylation and the rate-limiting steps of the reaction mechanism were identified. The para-hydroxylated substrates permit us to study the effect of a substituent (R) in the ca...
journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/BC.2000.041
更新日期：2000-04-01 00:00:00
abstract：:Rhomboids are ubiquitous intramembrane serine proteases that cleave transmembrane substrates. Their functions include growth factor signaling, mitochondrial homeostasis, and parasite invasion. A recent study revealed that the Escherichia coli rhomboid protease EcGlpG is essential for its extraintestinal pathogenic col...
journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/hsz-2018-0255
更新日期：2018-11-27 00:00:00
abstract：:The bioactivation of inorganic nitrite refers to the conversion of otherwise 'inert' nitrite to the diatomic signaling molecule nitric oxide (NO), which plays important roles in human physiology and disease, notably in the regulation of vascular tone and blood flow. While the most well-known sources of NO are the nitr...
journal_title：Biological chemistry
pub_type： 杂志文章,评审
doi：10.1515/hsz-2019-0349
更新日期：2019-12-18 00:00:00
abstract：:The hypoxia-inducible factor-1 (HIF-1) is a basic-helix-loop-helix (bHLH) heterodimeric transcription factor activated by reductions in oxygen concentration (hypoxia). Activated HIF-1 upregulates expression of genes involved in the adaptation of higher organisms to hypoxic conditions, caused by e.g. high altitude, ane...
journal_title：Biological chemistry
pub_type： 杂志文章,评审
doi：
更新日期：1997-07-01 00:00:00
abstract：:The physiological roles of the intracellular iron and redox regulatory systems are intimately linked. Iron is an essential trace element for most organisms, yet elevated cellular iron levels are a potent generator and amplifier of reactive oxygen species and redox stress. Proteins binding iron or iron-sulfur (Fe/S) cl...
journal_title：Biological chemistry
pub_type： 杂志文章,评审
doi：10.1515/hsz-2020-0237
更新日期：2020-11-26 00:00:00
abstract：:Malaria can be a very severe disease, particularly in young children, pregnant women (mostly in primipara), and malaria naïve adults, and currently ranks among the most prevalent infections in tropical and subtropical areas throughout the world. The widespread occurrence and the increased incidence of malaria in many ...
journal_title：Biological chemistry
pub_type： 杂志文章,评审
doi：10.1515/BC.2001.069
更新日期：2001-04-01 00:00:00
abstract：:Dipeptidyl peptidase-IV has been demonstrated to play a role in cancer biology by many authors. Since then, additional proteins possessing similar enzymatic activity have been described and their role in cancerogenesis has been hypothesized. To assess the complexity of these 'Dipeptidyl peptidase-IV activity and/or st...
journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/BC.2004.067
更新日期：2004-06-01 00:00:00
abstract：:Hsp70 chaperone systems play an essential role in the life cycle of many proteins not only in an hostile environment but also under normal growth conditions. In the course of evolution the diversification of functions was accompanied by an amplification of components of the Hsp70 system. Here strategies are reviewed h...
journal_title：Biological chemistry
pub_type： 杂志文章,评审
doi：
更新日期：1998-03-01 00:00:00
