Abstract:
:Two new fluorescent labels are presented that are optimized for excitation with He/Ne laser and red diode lasers. Application in FCS and labeling of proteins and oligomers are demonstrated. A strong rise of quantum yield and emission life time upon binding to biomolecules are characteristic features of the dyes.
journal_name
Biol Chemjournal_title
Biological chemistryauthors
Czerney P,Lehmann F,Wenzel M,Buschmann V,Dietrich A,Mohr GJdoi
10.1515/BC.2001.062keywords:
subject
Has Abstractpub_date
2001-03-01 00:00:00pages
495-8issue
3eissn
1431-6730issn
1437-4315journal_volume
382pub_type
杂志文章abstract::Cytochrome c oxidase from bovine heart contains seven binding sites for ATP or ADP and three additional for ADP only, as concluded from competition equilibrium dialysis binding studies. The isolated enzyme contains bound cholate which, in contrast to bound ATP, is only slowly exchanged by ADP (or ATP). The kinetics of...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/bchm.1998.379.3.335
更新日期:1998-03-01 00:00:00
abstract::Recent progress in the identification and partial characterization of novel genes encoding cysteine proteases of the papain family has considerably increased our knowledge of this family of enzymes. Kinetic data available to date for this large family indicate relatively broad, overlapping specificities for most enzym...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2003.094
更新日期:2003-06-01 00:00:00
abstract::The kallikrein-kinin and renin-angiotensin (KKS-RAS) systems represent two highly regulated proteolytic systems that are involved in several physiological and pathological processes. Although their protein-protein interactions can be studied using experimental approaches, it is difficult to differentiate between direc...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2010.046
更新日期:2010-04-01 00:00:00
abstract::The non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase catalyses the irreversible reaction of glyceraldehyde-3-phosphate to 3-phosphoglycerate by the reduction of NADP to NADPH. This is in contrast to the extensively analysed phosphorylating glyceraldehyde-3-phosphate dehydrogenases which catalyse the revers...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:
更新日期:1997-12-01 00:00:00
abstract::Characterization of protein damage during photosensitization of chlorin e6-treated cells was performed using the green fluorescent protein (GFP). The GFP-chromophore damage caused by singlet oxygen was studied in COS 7 kidney cells and E. coli bacteria following light irradiation. Electron spin resonance (ESR) reveale...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2000.153
更新日期:2000-12-01 00:00:00
abstract::The glutamyl endopeptidase family of enzymes from staphylococci has been shown to be important virulence determinants of pathogenic family members, such as Staphylococcus aureus. Previous studies have identified the N-terminus and residues from positions 185-195 as potentially important regions that determine the acti...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2010.116
更新日期:2010-10-01 00:00:00
abstract::As a crucial protein, the herpes simplex virus 1 (HSV-1) UL2 protein has been shown to take part in various stages of viral infection, nonetheless, its exact subcellular localization and transport molecular determinants are not well known thus far. In the present study, by using live cells fluorescent microscopy assay...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2016-0268
更新日期:2017-04-01 00:00:00
abstract::Interleukin-13 (IL-13) is a T-cell-derived pleiotropic cytokine of particular medical importance because of its critical role in the development of allergic asthma. The effects of IL-13 on its target cells are mediated through a dimeric transmembrane receptor (IL-13R), which shares the IL-4Ralpha subunit with the IL-4...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2007.036
更新日期:2007-03-01 00:00:00
abstract::DEAD-box helicases catalyze RNA duplex unwinding in an ATP-dependent reaction. Members of the DEAD-box helicase family consist of a common helicase core formed by two RecA-like domains. According to the current mechanistic model for DEAD-box mediated RNA unwinding, binding of RNA and ATP triggers a conformational chan...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2014-0277
更新日期:2015-08-01 00:00:00
abstract::Bradykinin (BK) is a vasorelaxant, algesic and inflammatory agent. Angiotensin II (AngII) is known to control vascular tone and promote growth, inflammation and artherogenesis. There is evidence for cross talking between BK and AngII receptors. Therefore, the effect of lack of kinin receptors was assessed in mice with...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2009.081
更新日期:2009-09-01 00:00:00
abstract::M.EcoRV is an alpha-adenine DNA methyltransferase. According to structure predictions, the enzyme consists of a catalytic domain, which has a structure similar to all other DNA-methyltransferases, and a smaller DNA-recognition domain. We have investigated this enzyme by random mutagenesis, using error-prone PCR, follo...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/bchm.1998.379.4-5.475
更新日期:1998-04-01 00:00:00
abstract::The efficient execution of apoptotic cell death with the clearance of apoptotic debris by phagocytes is a key regulatory mechanism ensuring tissue homeostasis. Failure in this execution program contributes to the pathogenesis of many human diseases. In this review, we describe the current knowledge regarding the inter...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2009.048
更新日期:2009-05-01 00:00:00
abstract::Glucose-dependent insulinotropic polypeptide (GIP) is an incretin hormone secreted by endocrine K-cells in response to nutrient absorption. In this study we have utilized a specific and enzymatically stable GIP receptor antagonist, (Pro3)GIP, to evaluate the contribution of endogenous GIP to insulin secretion and gluc...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2004.110
更新日期:2004-09-01 00:00:00
abstract::In mammals, betaine of the mitochondrial matrix is used in the cytosol by betaine-homocysteine S-methyltransferase for methionine synthesis. The resulting dimethylglycine is shuttled back into the mitochondrial matrix for further degradation. Nanospray tandem mass spectrometry and N-terminal amino acid sequencing of m...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2000.080
更新日期:2000-07-01 00:00:00
abstract::The lysosomal cysteine proteinase cathepsin B has been implicated in the progression of various human tumors including ovarian cancer. Included in this study were 63 patients with epithelial ovarian carcinoma. Follow-up information (median follow-up period 7 years) was available for all patients, among whom 42 (66.7%)...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2002.146
更新日期:2002-07-01 00:00:00
abstract::The surface of spherical, nonporous silica nanoparticles (SiO2-NPs) was modified with gadolinium (Gd) complexes, fluorophores, and cell-penetrating peptides to achieve multifunctionality on a single particle. The Gd surface concentrations were 9-16 μmol/g resulting in nanomaterials with high local longitudinal and tra...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2012-0251
更新日期:2013-01-01 00:00:00
abstract::Tissue kallikrein KLK1 and the kallikrein-related peptidases KLK2-15 are a subfamily of serine proteases that have defined or proposed roles in a range of central nervous system (CNS) and non-CNS pathologies. To further understand their potential activity in multiple sclerosis (MS), serum levels of KLK1, 6, 7, 8 and 1...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2008.085
更新日期:2008-06-01 00:00:00
abstract::Human kallikrein-related peptidases (KLKs), including the well-known prostate cancer biomarker, prostate-specific antigen (PSA-KLK3), along with 14 other serine proteinase KLK family members are now known to regulate cells by cleaving and activating members of the G-protein-coupled proteinase-activated receptor (PAR) ...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2014-0123
更新日期:2014-09-01 00:00:00
abstract::The enzymatic properties of glycosylphosphatidylinositol-specific phospholipase D (EC 3.1.4.50) were characterized using a 6,000-fold purified enzyme. This was obtained in 100 microg amounts from human serum with a recovery of 35%. Pure alkaline phosphatase containing one anchor moiety per molecule was used as substra...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2000.062
更新日期:2000-05-01 00:00:00
abstract::Mitochondria contain elaborate machineries for the import of precursor proteins from the cytosol. The translocase of the outer mitochondrial membrane (TOM) performs the initial import of precursor proteins and transfers the precursors to downstream translocases, including the presequence translocase and the carrier tr...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2016-0145
更新日期:2016-11-01 00:00:00
abstract::Lipids serve many distinct functions in cellular homeostasis such as membrane organization, as a platform for membrane function and protein/protein or protein/lipid interaction, energy storage, as well as secondary messengers in signal transduction. Perturbations in lipid homeostasis may result in abnormal cellular fu...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2015-0207
更新日期:2015-12-01 00:00:00
abstract::Glycoconjugates have a whole spectrum of biological roles, from those that appear trivial to those that are crucial. Results accumulated in the past years indicate they might also play an important role in the response to stress, a complex physiological response of the human organism to various threats. We have recent...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.1999.058
更新日期:1999-04-01 00:00:00
abstract::Drug-induced phospholipidosis is a lysosomal storage disorder characterized by excessive accumulation of phospholipids. Its cellular mechanism is still not well understood, but it is known that cationic amphiphilic drugs can induce it. These drugs have a hydrophilic amine head group that can be protonated in the endol...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2019-0270
更新日期:2019-12-18 00:00:00
abstract::To facilitate evolutionary and forensic studies of DNA polymorphisms on the Y chromosome, we devised a multiplex amplification procedure for short-tandem-repeat (STR) loci. Four tetranucleotide STR loci (DYS19, DYS390, DYS391, and DYS393) were simultaneously amplified with FAM-labeled primers and genotypes were determ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/bchm.1997.378.8.923
更新日期:1997-08-01 00:00:00
abstract::Meprins are zinc-endopeptidases of the astacin family, which are expressed as membrane-bound or secreted forms in renal and intestinal brush-border membranes of mouse, rat and man. There are two types of meprin subunits, alpha and beta, which form disulfide-bonded homo- and heterodimers; further oligomerization is med...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2003.092
更新日期:2003-05-01 00:00:00
abstract::The three-dimensional structure of pterin-4a-carbinolamine dehydratase (PCD) from Pseudomonas aeruginosa has been solved. Based on this we have investigated the roles of putative active center residues through functional replacement by site-directed mutagenesis. Three histidines, His73, His74 and His91, appear to be i...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/bchm.1998.379.12.1427
更新日期:1998-12-01 00:00:00
abstract::The thyroglobulin type-1 (Tg-1) domain is a protein module that occurs in a variety of secreted and membrane proteins and is recognised as a potent inhibitor of cysteine peptidases. We present here some properties of the Tg-1 domain of human testican, a modularly organised proteoglycan secreted mainly by brain cells, ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2005.010
更新日期:2005-01-01 00:00:00
abstract::The sphingolipid sphingosine-1-phosphate (S1P) has various functions in immune cell biology, regulating survival, proliferation, and, most prominently, migration. S1P couples to five G protein-coupled receptors (S1PR1-5) to transduce its effects on immune cell function. Expression of S1PR4 is restricted to immune cell...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2014-0271
更新日期:2015-06-01 00:00:00
abstract::We studied the mode of regulation of the activity of mature cathepsin B (CB) by L-cysteine and some related thiols. The activity of CB with Z-Arg-Arg-NHMec as substrate was gradually inhibited over a range of increasing concentration of Cys, Cys methyl ester (CysOMe), Cys ethyl ester (CysOEt), N-acetyl-Cys (N-AcCys) a...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.1999.069
更新日期:1999-05-01 00:00:00
abstract::The production and processing of ribosomal RNA is a complex and well-coordinated nucleolar process for ribosome biogenesis. Progress in understanding nucleolar structure and function has lead to the unexpected discovery of the nucleolus as a highly sensitive sensor of cellular stress and an important regulator of the ...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2013-0153
更新日期:2013-09-01 00:00:00