Abstract:
:Understanding the molecular mechanisms behind extreme temperature stability is of relevance for the protein folding problem and for designing proteins for industrial and medical applications. A powerful approach for understanding the structural basis of thermostability is the comparison of high resolution structures of homologous proteins from mesophiles and thermophiles. The 1.75 A crystal structure of Thermotoga maritima 1[4Fe-4S] ferredoxin was compared with those of mesophilic ferredoxins. Detailed analysis of structural differences reveals that thermostability is achieved without large changes of the overall polypeptide chain folding. The most striking differences include the formation of additional hydrogen bonding networks involving both side-chain and main-chain atoms. These networks are mainly connecting turns and strongly fix the N-terminus to the central core of the protein, increasing the overall rigidity of Thermotoga maritima ferredoxin. Other possibly stabilizing factors are the shortening of a solvent exposed surface loop, the increased content of alanines in the second alpha-helix, and the replacement of three residues close to the iron-sulfur cluster, which are in energetically unfavourable conformations in other ferredoxins, by glycines.
journal_name
Biol Chemjournal_title
Biological chemistryauthors
Macedo-Ribeiro S,Darimont B,Sterner Rsubject
Has Abstractpub_date
1997-03-01 00:00:00pages
331-6issue
3-4eissn
1431-6730issn
1437-4315journal_volume
378pub_type
杂志文章abstract::Fluorescence correlation spectroscopy (FCS) was used in monitoring human parvovirus B19 virus-like particle (VLP) antibody complexes from acute phase and past-immunity serum samples. The Oregon Green 488-labeled VLPs gave an average diffusion coefficient of 1.7 x 10(-7) cm2 s(-1) with an apparent hydrodynamic radius o...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2004.011
更新日期:2004-01-01 00:00:00
abstract::Recent progress in the identification and partial characterization of novel genes encoding cysteine proteases of the papain family has considerably increased our knowledge of this family of enzymes. Kinetic data available to date for this large family indicate relatively broad, overlapping specificities for most enzym...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2003.094
更新日期:2003-06-01 00:00:00
abstract::In pancreatic acinar cells stimulation of different intracellular pathways leads to different patterns of Ca2+ signaling. Bombesin induces activation of both phosphatidylinositol 4,5-bisphosphate (PIP2)-specific phospholipase C (PLC) and phospholipase D (PLD). The latter leads to generation of diacylglycerol (DAG) in ...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.1999.111
更新日期:1999-07-01 00:00:00
abstract::A large number of prostate cancer (PCa) patients receive treatment without significant benefits, strengthening the need for accurate prognosis, which can be supported by the study of miRNAs. In silico specificity analysis was performed for the identification of miRNAs able to regulate KLK2 and KLK4 expression. Total R...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2014-0150
更新日期:2014-09-01 00:00:00
abstract::Consumption of green tea is associated with a decrease in cardiovascular mortality. The beneficial health effects of green tea are attributed in part to polyphenols, organic compounds found in tea that lower blood pressure, reduce body fat, decrease LDL cholesterol, and inhibit inflammation. We hypothesized that epiga...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2008.095
更新日期:2008-07-01 00:00:00
abstract::Latent episomal genomes of Epstein-Barr virus, a human gammaherpesvirus, represent a suitable model system for studying replication and methylation of chromosomal DNA in mammals. We analyzed the methylation patterns of CpG dinucleotides in the latent origin of DNA replication of Epstein-Barr virus using automated fluo...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2000.014
更新日期:2000-02-01 00:00:00
abstract::Five moeA mutants were generated by replacing some conserved amino acids of MoeA by site-directed mutagenesis. The mutants were assayed for the ability to restore in vivo nitrate reductase activity of the moeA mutant Escherichia coli JRG97 and in vitro Neurospora crassa nit-1 nitrate reductase activity. The replacemen...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2002.034
更新日期:2002-02-01 00:00:00
abstract::In mammals, betaine of the mitochondrial matrix is used in the cytosol by betaine-homocysteine S-methyltransferase for methionine synthesis. The resulting dimethylglycine is shuttled back into the mitochondrial matrix for further degradation. Nanospray tandem mass spectrometry and N-terminal amino acid sequencing of m...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2000.080
更新日期:2000-07-01 00:00:00
abstract::Stromal cell-derived factor-1alpha (SDF-1alpha) is a strong migratory stimulant for hematopoietic stem and progenitor cells (HSPCs). The hematopoietic cytokines thrombopoietin (TPO), Flt3-ligand (FL), stem cell factor (SCF) and interleukin 11 (IL-11) are able to stimulate amplification of primitive murine hematopoieti...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2008.099
更新日期:2008-07-01 00:00:00
abstract::The saline extract of Bauhinia bauhinioides dry seeds was shown to inhibit cruzipain, a cysteine proteinase from Trypanosoma cruzi. The inhibitory activity was assigned to a protein with 164 amino acid residues and molecular mass of 18 034 Da that was purified by chromatography on DEAE-Sephadex, trypsin-Sepharose (rem...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2001.103
更新日期:2001-05-01 00:00:00
abstract::The formation of N-glycosidic linkages of glycoproteins involves the ordered assembly of the common Glc3Man9GlcNAc2 core-oligosaccharide on the lipid carrier dolichyl pyrophosphate. Whereas early mannosylation steps occur on the cytoplasmic side of the endoplasmic reticulum with GDP-Man as donor, the final reactions f...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2001.039
更新日期:2001-02-01 00:00:00
abstract::The periplasmic ligand-binding protein ChoX is part of the ABC transport system ChoVWX that imports choline as a nutrient into the soil bacterium Sinorhizobium meliloti. We have recently reported the crystal structures of ChoX in complex with its ligands choline and acetylcholine and the structure of a fully closed bu...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2009.113
更新日期:2009-11-01 00:00:00
abstract::Vaccination with either exogenous hepatitis B surface antigen (HBsAg) lipoprotein particles without adjuvants, or plasmid DNA encoding secreted small HBsAg stimulate long-lasting, potent antibody responses in H-2d (BALB/c) and C57Bl/6 (H-2b) mice. Vaccination with exogenous HBsAg primes MHC-I restricted cytotoxic T ly...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.1999.039
更新日期:1999-03-01 00:00:00
abstract::In the SalI system, endonuclease activity can be only achieved in the presence of a functional modification gene. Thus, the DNA methyltransferase is involved in the control of restriction. By fusion of the restriction gene of the SalI system to the modification gene of the isospecific HgiDII system a hybrid type II re...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:
更新日期:1998-04-01 00:00:00
abstract::Matrix metalloproteinases (MMPs) are a family of zinc endopeptidases that have been implicated in various disease processes. Different classes of MMP inhibitors, including hydroxamic acids, phosphinic acids and thiols, have been previously described. Most of these mimic peptides and most likely bind in a similar way t...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2001.159
更新日期:2001-08-01 00:00:00
abstract::The conversion of cellular prion protein (PrP(C)) into its pathological isoform (PrP(Sc)) conveys an increase in hydrophobicity and induces a partial resistance to proteinase K (PK). Interestingly, co-incubation with high copper ion concentrations also modifies the solubility of PrP(c) and induces a partial PK resista...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2004.090
更新日期:2004-08-01 00:00:00
abstract::Biofilms are a ubiquitous mode of microbial life and display an increased tolerance to different stresses. Inside biofilms, cells may experience both externally applied stresses and internal stresses that emerge as a result of growth in spatially structured communities. In this review, we discuss the spatial scales of...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2020-0213
更新日期:2020-11-26 00:00:00
abstract::Few therapeutic options are offered to treat inflammation and alveolar wall destruction in emphysema. The effect of recombinant human pre-elafin, an elastase inhibitor, was evaluated in porcine pancreatic elastase (PPE)-induced emphysema in C57BL/6 mice. In a first protocol, mice received a single instillation of pre-...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2006.114
更新日期:2006-07-01 00:00:00
abstract::Zinc (Zn2+) is the most abundant trace element in cells and is essential for a vast number of catalytic, structural, and regulatory processes. Mounting evidence indicates that like calcium (Ca2+), intracellular Zn2+ pools are redistributed for specific cellular functions. This occurs through the regulation of 24 Zn2+ ...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2012-0128
更新日期:2012-07-01 00:00:00
abstract::Based on its monophenolic structure and given its pharmacological and toxicological importance, the ability of tyrosinase to oxidize acetaminophen was studied for the first time. Progress curves showed a transient phase characteristic of the monophenolase activity of tyrosinase prior to attaining the steady-state. The...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2002.217
更新日期:2002-12-01 00:00:00
abstract::Glycoconjugates have a whole spectrum of biological roles, from those that appear trivial to those that are crucial. Results accumulated in the past years indicate they might also play an important role in the response to stress, a complex physiological response of the human organism to various threats. We have recent...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.1999.058
更新日期:1999-04-01 00:00:00
abstract::Loss of genetic material from chromosome 3p21.3 is one of the most common and earliest events in the pathogenesis of lung cancer and many other solid tumors. The chromosomal area 3p21.3 is thought to harbor at least one important tumor suppressor gene, which, despite many years of investigation, has remained elusive. ...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2002.097
更新日期:2002-06-01 00:00:00
abstract::Hyperpolarization-activated cation currents, termed I(h), are observed in a variety of neurons. Four members of a gene family encoding hyperpolarization-activated cyclic-nucleotide-gated cation channels (HCN1-4) have been cloned. The regional expression and cellular localization of the four HCN channel types in mouse ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.1999.121
更新日期:1999-07-01 00:00:00
abstract::The central process for the transfer of the genetic information from the nucleic acid world into the structure of proteins is the ribosomal elongation cycle, where the sequence of codons is translated into the sequence of amino acids. The nascent polypeptide chain is elongated by one amino acid during the reactions of...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/bchm.1998.379.7.753
更新日期:1998-07-01 00:00:00
abstract::During the last decade, an increasing number of new enzymes containing glycyl radicals in their active sites have been identified and biochemically characterised. These include benzylsuccinate synthase (Bss), 4-hydroxyphenylacetate decarboxylase (Hpd) and the coenzyme B12-independent glycerol dehydratase (Gdh). These ...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2005.114
更新日期:2005-10-01 00:00:00
abstract::A FAD and [4Fe-4S]cluster-containing enzyme from Clostridium aminobutyricum catalyses the reversible dehydration of 4-hydroxybutyryl-CoA to crotonyl-CoA which involves the cleavage of an unactivated C-H bond at the beta-carbon. Transient oxidation of the substrate to an enoxy radical by FAD might facilitate the remova...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/bchm.1997.378.8.843
更新日期:1997-08-01 00:00:00
abstract::We have previously shown that short double-stranded RNAs of specific sequence induce phosphorylation in the activation loop of splicing variant D of the transforming growth factor beta-activated protein kinase 1 (TAK1-D). Here, we further characterize this novel function of TAK1-D and the mechanisms of this dsRNA-trig...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2009.046
更新日期:2009-05-01 00:00:00
abstract::This article presents a historical account of the discovery of reversible protein phosphorylation. The process was uncovered in the mid-1950s in a study designed to elucidate the complex hormonal regulation of skeletal muscle glycogen phosphorylase. This enzyme was originally thought to be regulated by AMP now known t...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2010.011
更新日期:2010-02-01 00:00:00
abstract::Historically considered as accessory cells to neurons, there is an increasing interest in the role of astrocytes in normal and pathological conditions. Astrocytes are involved in neurotransmitter recycling, antioxidant supply, ion buffering and neuroinflammation, i.e. a lot of the same pathways that go astray in Alzhe...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2019-0140
更新日期:2019-08-27 00:00:00
abstract::The GAGA box of the rat serine protease inhibitor 2 (spi 2) genes not only acts as a basal promoter element, but also mediates transcriptional activation by growth hormone and interleukin-6. The GAGA box is separated from the TATA box by only 12 bp, and this close association is required for efficient transcription. H...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2000.127
更新日期:2000-11-01 00:00:00