Establishment of a hybrid SalI-HgiDII type II restriction-modification system.

abstract：:We recently reported that human melanoma cells, but not benign melanocytes, aberrantly express kallikrein-related peptidase 7 (KLK7). Here, we show a KLK7 overexpression-mediated decrease of cell adhesion to extracellular matrix binding proteins, associated with downregulation of α5/β1/αv/β3 integrin expression. We al...

journal_title：Biological chemistry

authors： Haddada M,Draoui H,Deschamps L,Walker F,Delaunay T,Brattsand M,Magdolen V,Darmoul D

更新日期：2018-09-25 00:00:00

abstract：:A deletion mutant (V314) of the calcineurin A subunit was constructed using site-directed mutagenesis. Its phosphatase activity and function were then characterized. The V314 deletion significantly altered the phosphatase activity, which was more than ten times higher than that of wild-type calcineurin, the calcineuri...

journal_title：Biological chemistry

authors： Yan L,Wei Q

更新日期：1999-11-01 00:00:00

abstract：:The neurobiological mechanisms of obesity-induced peripheral neuropathy are poorly understood. We evaluated the role of Sigma-1 receptor (Sig-1R) and NMDA receptor (NMDARs) in the spinal cord in peripheral neuropathy using an animal model of high fat diet-induced diabetes. We examined the expression of Sig-1R and NMDA...

journal_title：Biological chemistry

authors： Song T,Zhao J,Ma X,Zhang Z,Jiang B,Yang Y

更新日期：2017-09-26 00:00:00

abstract：:Type I allergy, a hypersensitivity disease affecting almost 20% of the population worldwide, is based on the IgE recognition of otherwise harmless antigens (i.e., allergens). Allergen-induced crosslink of effector cell-bound IgE antibodies leads to the release of biological mediators and thus to immediate disease symp...

journal_title：Biological chemistry

authors： Valenta R,Vrtala S,Focke-Tejkl M,Bugajska-Schretter,Ball T,Twardosz A,Spitzauer S,Grönlund H,Kraft D

更新日期：1999-07-01 00:00:00

abstract：:Disulfide bonds play a critical role in a variety of structural and mechanistic processes associated with proteins inside the cells and in the extracellular environment. The thioredoxin family of proteins like thioredoxin (Trx), glutaredoxin (Grx) and protein disulfide isomerase, are involved in the formation, transfe...

journal_title：Biological chemistry

authors： Sousa SF,Neves RPP,Waheed SO,Fernandes PA,Ramos MJ

更新日期：2019-04-24 00:00:00

abstract：:Kallikrein 6 (KLK6) is a trypsin-like serine peptidase whose relevance in various types of cancers is currently being explored. Previous studies have shown that KLK6 mRNA is upregulated in colon and gastric cancers; however, the regulatory mechanisms and phenotypic consequences of this upregulation are largely unknown...

journal_title：Biological chemistry

authors： Henkhaus RS,Gerner EW,Ignatenko NA

更新日期：2008-06-01 00:00:00

abstract：:In this study, we tested the biological activity of a novel acylated form of (Pro3)glucose-dependent insulinotropic polypetide [(Pro3)GIP] prepared by conjugating palmitic acid to Lys16 to enhance its efficacy in vivo by promoting binding to albumin and extending its biological actions. Like the parent molecule (Pro3)...

journal_title：Biological chemistry

authors： Gault VA,Hunter K,Irwin N,Greer B,Green BD,Harriott P,O'Harte FP,Flatt PR

更新日期：2007-02-01 00:00:00

abstract：:We have previously shown that compatible organic osmolytes, such as betaine, myo-inositol and taurine, are part of the stress response of normal human keratinocytes (NHKs) to ultraviolet B (UVB) radiation. In this regard, we tested human HaCaT keratinocytes as a surrogate cell line for NHK. HaCaT cells osmo-dependentl...

journal_title：Biological chemistry

authors： Warskulat U,Brookmann S,Reinen A,Häussinger D

更新日期：2007-12-01 00:00:00

abstract：:Group II introns are autocatalytic RNAs which self-splice in vitro. However, in vivo additional protein factors might be involved in the splicing process. We used an affinity chromatography method called 'StreptoTag' to identify group II intron binding proteins from Saccharomyces cerevisiae. This method uses a hybrid ...

journal_title：Biological chemistry

authors： Böck-Taferner P,Wank H

更新日期：2004-07-01 00:00:00

abstract：:While the role of C2-ceramide in the induction of programmed cell death (PCD) in animal systems has been well documented, little is known of its role in plant cells. Here we show that C2-ceramide induces PCD in Arabidopsis suspension cultures, which is preceded by the generation of a calcium transient and an increase ...

journal_title：Biological chemistry

authors： Townley HE,McDonald K,Jenkins GI,Knight MR,Leaver CJ

更新日期：2005-02-01 00:00:00

abstract：:Glucose-dependent insulinotropic polypeptide (GIP) is an incretin hormone secreted by endocrine K-cells in response to nutrient absorption. In this study we have utilized a specific and enzymatically stable GIP receptor antagonist, (Pro3)GIP, to evaluate the contribution of endogenous GIP to insulin secretion and gluc...

journal_title：Biological chemistry

authors： Irwin N,Gault VA,Green BD,Greer B,McCluskey JT,Harriott P,O'Harte FP,Flatt PR

更新日期：2004-09-01 00:00:00

abstract：:We present a method that allows preparing long DNA containing defined mismatches without the use of gel electrophoretic or chromatographic purification steps. The preparation starts with the synthesis of two PCR products, which are identical except for those positions that will later form the mismatches. One of the PC...

journal_title：Biological chemistry

authors： Thomas E,Pingoud A,Friedhoff P

更新日期：2002-09-01 00:00:00

abstract：:Hyperpolarization-activated cation currents, termed I(h), are observed in a variety of neurons. Four members of a gene family encoding hyperpolarization-activated cyclic-nucleotide-gated cation channels (HCN1-4) have been cloned. The regional expression and cellular localization of the four HCN channel types in mouse ...

journal_title：Biological chemistry

authors： Moosmang S,Biel M,Hofmann F,Ludwig A

更新日期：1999-07-01 00:00:00

abstract：:A controlled interaction of materials with their surrounding biological environment is of great interest in many fields. Multifunctional coatings aim to provide simultaneous modulation of several biological signals. They can consist of various combinations of bioactive, and bioinert components as well as of reporter m...

journal_title：Biological chemistry

authors： Pagel M,Beck-Sickinger AG

更新日期：2017-01-01 00:00:00

abstract：:A large number of prostate cancer (PCa) patients receive treatment without significant benefits, strengthening the need for accurate prognosis, which can be supported by the study of miRNAs. In silico specificity analysis was performed for the identification of miRNAs able to regulate KLK2 and KLK4 expression. Total R...

journal_title：Biological chemistry

authors： Avgeris M,Stravodimos K,Scorilas A

更新日期：2014-09-01 00:00:00

abstract：:The kallikrein-kinin and renin-angiotensin (KKS-RAS) systems represent two highly regulated proteolytic systems that are involved in several physiological and pathological processes. Although their protein-protein interactions can be studied using experimental approaches, it is difficult to differentiate between direc...

journal_title：Biological chemistry

authors： Stoka V,Turk V

更新日期：2010-04-01 00:00:00

abstract：:Kallikrein-related peptidase 2 (KLK2) degrades insulin-like growth factor (IGF)-binding protein-3 (IGFBP-3) in vitro. IGFBP-3 forms complexes with IGFs, preventing them from binding to their receptors and stimulating cell proliferation and survival. IGF-independent actions have also been described for IGFBP-3. The deg...

journal_title：Biological chemistry

authors： Hekim C,Riipi T,Weisell J,Närvänen A,Koistinen R,Stenman UH,Koistinen H

更新日期：2010-04-01 00:00:00

abstract：:We present a rapid and simple method for the sexing of human and other primate DNA. Homologous introns from the X- and Y-linked zinc finger protein genes (ZFX and ZFY) are PCR amplified with one primer pair. An Alu element insertion in the ZFX intron results in a size difference in the amplicons which is resolved by a...

journal_title：Biological chemistry

authors： Wilson JF,Erlandsson R

更新日期：1998-10-01 00:00:00

abstract：:The transcription factor E2F1 plays a decisive role in the G1/S and G2/M checkpoint transitions of proliferating cells. Because cells are arrested at these checkpoints after heat shock it was of interest to test heat shock effects on E2F1 activity. In human A549 cells, heat shock (44 degrees C, 30 min) caused an immed...

journal_title：Biological chemistry

authors： Gerullis D,Rensing L,Beyersmann D

更新日期：2003-01-01 00:00:00

abstract：:Matrix metalloproteinases (MMPs) are a family of zinc endopeptidases that have been implicated in various disease processes. Different classes of MMP inhibitors, including hydroxamic acids, phosphinic acids and thiols, have been previously described. Most of these mimic peptides and most likely bind in a similar way t...

journal_title：Biological chemistry

authors： Grams F,Brandstetter H,D'Alò S,Geppert D,Krell HW,Leinert H,Livi V,Menta E,Oliva A,Zimmermann G,Gram F,Brandstetter H,D'Alò S,Geppert D,Krell HW,Leinert H,Livi VMenta E,Oliva A,Zimmermann G

更新日期：2001-08-01 00:00:00

abstract：:Molecular chaperones are well known as facilitators of protein folding and assembly. However, in recent years multiple chaperone-assisted degradation pathways have also emerged, including CAP (chaperone-assisted proteasomal degradation), CASA (chaperone-assisted selective autophagy), and CMA (chaperone-mediated autoph...

journal_title：Biological chemistry

authors： Kettern N,Dreiseidler M,Tawo R,Höhfeld J

更新日期：2010-05-01 00:00:00

abstract：:L-Arginine:glycine amidinotransferase (AT) catalyzes the committed step in creatine biosynthesis by formation of guanidinoacetic acid, the direct precursor of creatine. The X-ray structure of the human enzyme shows a novel fold with fivefold pseudosymmetry of beta beta alphabeta-modules. These modules enclose the acti...

journal_title：Biological chemistry

authors： Humm A,Fritsche E,Steinbacher S

更新日期：1997-03-01 00:00:00

abstract：:Mitochondria contain elaborate machineries for the import of precursor proteins from the cytosol. The translocase of the outer mitochondrial membrane (TOM) performs the initial import of precursor proteins and transfers the precursors to downstream translocases, including the presequence translocase and the carrier tr...

journal_title：Biological chemistry

authors： Straub SP,Stiller SB,Wiedemann N,Pfanner N

更新日期：2016-11-01 00:00:00

abstract：:The TNF family molecule RANKL and its receptor RANK are key regulators of bone remodeling, lymph node formation, and mammary gland development during pregnancy. RANKL and RANK are also expressed in the central nervous systems (CNS). However, the functional relevance of RANKL/RANK in the brain was entirely unknown. Rec...

journal_title：Biological chemistry

authors： Hanada R,Hanada T,Penninger JM

更新日期：2010-12-01 00:00:00

abstract：:92 kDa and 72 kDa gelatinases, two neutral proteinases exhibiting elastinolytic activity and secreted as zymogens by aortic smooth muscle cells, were shown to bind to insoluble elastin. The active form of each enzyme interacted with substrate more avidly than latent form. Once bound to insoluble elastin, 92 kDa progel...

journal_title：Biological chemistry

authors： Emonard H,Hornebeck W

更新日期：1997-03-01 00:00:00

abstract：:The hair follicle consists of a complex system of multiple tissue compartments that are clearly distinguishable by their morphology and type of differentiation. We have synthesized hair follicle-specific keratins from the companion layer (K6hf, K17) and the hair cortex (Ha1, Hb3, Hb6) in Escherichia coli. The assembly...

journal_title：Biological chemistry

authors： Hofmann I,Winter H,Mücke N,Langowski J,Schweizer J

更新日期：2002-09-01 00:00:00

abstract：:Small RNAs such as microRNAs (miRNAs), short interfering RNAs (siRNAs) or Piwi-interacting RNAs (piRNAs) are important regulators of gene expression in various organisms. Small RNAs bind to a member of the Argonaute protein family and are incorporated into larger structures that mediate diverse gene silencing events. ...

journal_title：Biological chemistry

authors： Dueck A,Meister G

更新日期：2014-06-01 00:00:00

abstract：:The bifunctional protein DCoH (dimerization cofactor of HNF-1) is a transcriptional coactivator of homeodomain proteins and a cytoplasmic enzyme. Here we report on the isolation, nucleotide sequence and genomic structure of the chicken and rat DCoH genes. They have an unusual first exon coding for a single amino acid....

journal_title：Biological chemistry

authors： Bossow S,Riepl S,Igo-Kemenes T

更新日期：1998-11-01 00:00:00

abstract：:Invadolysin is a novel metalloprotease conserved amongst metazoans that is essential for life in Drosophila. We previously showed that invadolysin was essential for the cell cycle and cell migration, linking to metabolism through a role in lipid storage and interaction with mitochondrial proteins. In this study we dem...

journal_title：Biological chemistry

authors： Chang CW,Abhinav K,Di Cara F,Panagakou I,Vass S,Heck MM

更新日期：2017-03-01 00:00:00

abstract：:Transforming growth factor-beta1 (TGF-beta1) has a biphasic effect on the growth of renal epithelial cells. In transformed cells, TGF-beta1 appears to accelerate the proliferation of malignant cells. The diverse cellular functions of TGF-beta1 are regulated by three high-affinity serine/threonine kinase receptors, nam...

journal_title：Biological chemistry

authors： Moodley R,Snyman C,Odhav B,Bhoola KD

更新日期：2005-04-01 00:00:00