Abstract:
:Mitochondria contain elaborate machineries for the import of precursor proteins from the cytosol. The translocase of the outer mitochondrial membrane (TOM) performs the initial import of precursor proteins and transfers the precursors to downstream translocases, including the presequence translocase and the carrier translocase of the inner membrane, the mitochondrial import and assembly machinery of the intermembrane space, and the sorting and assembly machinery of the outer membrane. Although the protein translocases can function as separate entities in vitro, recent studies revealed a close and dynamic cooperation of the protein import machineries to facilitate efficient transfer of precursor proteins in vivo. In addition, protein translocases were found to transiently interact with distinct machineries that function in the respiratory chain or in the maintenance of mitochondrial membrane architecture. Mitochondrial protein import is embedded in a regulatory network that ensures protein biogenesis, membrane dynamics, bioenergetic activity and quality control.
journal_name
Biol Chemjournal_title
Biological chemistryauthors
Straub SP,Stiller SB,Wiedemann N,Pfanner Ndoi
10.1515/hsz-2016-0145subject
Has Abstractpub_date
2016-11-01 00:00:00pages
1097-1114issue
11eissn
1431-6730issn
1437-4315pii
/j/bchm.just-accepted/hsz-2016-0145/hsz-2016-0145.journal_volume
397pub_type
杂志文章,评审abstract::Trypsin and chymotrypsin have similar tertiary structures, although very different substrate specificities. Trypsin hydrolyzes peptides at Lys/Arg residues while chymotrypsin recognizes large hydrophobic residues. Recent work has shown that trypsin is not converted into a protease with chymotrypsin-like activity when ...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:
更新日期:1996-07-01 00:00:00
abstract::Hyperpolarization-activated cation currents, termed I(h), are observed in a variety of neurons. Four members of a gene family encoding hyperpolarization-activated cyclic-nucleotide-gated cation channels (HCN1-4) have been cloned. The regional expression and cellular localization of the four HCN channel types in mouse ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.1999.121
更新日期:1999-07-01 00:00:00
abstract::In pancreatic acinar cells stimulation of different intracellular pathways leads to different patterns of Ca2+ signaling. Bombesin induces activation of both phosphatidylinositol 4,5-bisphosphate (PIP2)-specific phospholipase C (PLC) and phospholipase D (PLD). The latter leads to generation of diacylglycerol (DAG) in ...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.1999.111
更新日期:1999-07-01 00:00:00
abstract::Cytochrome c oxidase from bovine heart contains seven binding sites for ATP or ADP and three additional for ADP only, as concluded from competition equilibrium dialysis binding studies. The isolated enzyme contains bound cholate which, in contrast to bound ATP, is only slowly exchanged by ADP (or ATP). The kinetics of...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/bchm.1998.379.3.335
更新日期:1998-03-01 00:00:00
abstract::Nanomolar concentrations of human amylin promote death of RINm5F cells in a time- and concentrationdependent manner. Morphological changes of chromatin integrity suggest that cells are predominantly undergoing apoptosis. Human amylin induces significant activation of caspase-3 and strong and sustained phosphorylation ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2002.196
更新日期:2002-11-01 00:00:00
abstract::There is increasing evidence that apoptosis and necrosis represent only two of several possible ways for cells to die. These two types of demise can occur simultaneously in tissues or cell cultures exposed to the same stimulus, and often local metabolic conditions and the intensity of the same initial insult decide th...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.1999.129
更新日期:1999-09-01 00:00:00
abstract::Glutaminyl cyclases (QC) catalyze the intramolecular cyclization of N-terminal glutamine residues of peptides and proteins. For a comparison of the substrate specificity of human and papaya QC enzymes, a novel continuous assay was established by adapting an existing discontinuous method. Specificity constants (kcat/Km...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2003.175
更新日期:2003-12-01 00:00:00
abstract::Eukaryotic organisms have evolved complex and robust cellular stress response pathways to ensure maintenance of proteostasis and survival during fluctuating environmental conditions. Highly conserved stress response pathways can be triggered and coordinated at the cell-autonomous and cell-nonautonomous level by proteo...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2019-0385
更新日期:2020-08-27 00:00:00
abstract::We recently reported that human melanoma cells, but not benign melanocytes, aberrantly express kallikrein-related peptidase 7 (KLK7). Here, we show a KLK7 overexpression-mediated decrease of cell adhesion to extracellular matrix binding proteins, associated with downregulation of α5/β1/αv/β3 integrin expression. We al...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2017-0339
更新日期:2018-09-25 00:00:00
abstract::Alternative splicing is a process by which multiple messenger RNAs (mRNAs) are generated from a single pre-mRNA, resulting in functionally distinct protein products. This is accomplished by the differential recognition of splice sites in the pre-mRNA, often regulated in a tissue- or development-specific manner. Altern...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2005.143
更新日期:2005-12-01 00:00:00
abstract::Mitochondria are indispensable cellular organelles providing ATP and numerous other essential metabolites to ensure cell survival. Reactive oxygen species (ROS), which are formed as side reactions during oxidative phosphorylation or by external agents, induce molecular damage in mitochondrial proteins, lipids/membrane...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2017-0206
更新日期:2017-12-20 00:00:00
abstract::Interleukin-13 (IL-13) is a T-cell-derived pleiotropic cytokine of particular medical importance because of its critical role in the development of allergic asthma. The effects of IL-13 on its target cells are mediated through a dimeric transmembrane receptor (IL-13R), which shares the IL-4Ralpha subunit with the IL-4...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2007.036
更新日期:2007-03-01 00:00:00
abstract::A major challenge in the research on membrane-anchored and integral membrane protein complexes is to obtain these in a functionally active, water-soluble, and monodisperse form. This requires the incorporation of the membrane proteins into a native-like membrane or detergent micelle that mimics the properties of the o...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2009.091
更新日期:2009-08-01 00:00:00
abstract::The accumulation of cholesterol is a general feature of cancer tissue, and recent evidence suggests that cholesterol plays critical roles in the progression of cancers, including breast, prostate, and colorectal cancers. The dysregulation of metabolic pathways, including those involved in cholesterol biosynthesis, is ...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2014-0194
更新日期:2015-01-01 00:00:00
abstract::Protein phosphatase 2A (PP2A) constitutes one of the major families of protein serine/threonine phosphatases found in all eukaryotic cells. PP2A holoenzymes are composed of a catalytic subunit complexed with a structural regulatory subunit of 65 kDa. These core subunits associate with regulatory subunits of various si...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.1999.139
更新日期:1999-09-01 00:00:00
abstract::Transforming growth factor-beta1 (TGF-beta1) has a biphasic effect on the growth of renal epithelial cells. In transformed cells, TGF-beta1 appears to accelerate the proliferation of malignant cells. The diverse cellular functions of TGF-beta1 are regulated by three high-affinity serine/threonine kinase receptors, nam...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2005.045
更新日期:2005-04-01 00:00:00
abstract::Drug-induced phospholipidosis is a lysosomal storage disorder characterized by excessive accumulation of phospholipids. Its cellular mechanism is still not well understood, but it is known that cationic amphiphilic drugs can induce it. These drugs have a hydrophilic amine head group that can be protonated in the endol...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2019-0270
更新日期:2019-12-18 00:00:00
abstract::A putative operon of the ribosomal proteins S20 and Thx has been determined in a 1.4 kb sequenced region of T. thermophilus genomic DNA. Both genes have a promoter sequence 29 nt upstream of ORF1, possess their own Shine-Dalgarno motifs (GGAG) and are separated by only 9 nucleotides, a feature characteristic of the co...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2001.126
更新日期:2001-07-01 00:00:00
abstract::The GAGA box of the rat serine protease inhibitor 2 (spi 2) genes not only acts as a basal promoter element, but also mediates transcriptional activation by growth hormone and interleukin-6. The GAGA box is separated from the TATA box by only 12 bp, and this close association is required for efficient transcription. H...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2000.127
更新日期:2000-11-01 00:00:00
abstract::Cathepsin E (CE) is an intracellular aspartic proteinase implicated in various physiological and pathological processes, yet its actual roles in vivo remain elusive. To assess the physiological significance of CE expression in tumor cells, human CE was stably expressed in human prostate carcinoma ALVA101 cells express...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2007.154
更新日期:2007-11-01 00:00:00
abstract::M.EcoRV is an alpha-adenine DNA methyltransferase. According to structure predictions, the enzyme consists of a catalytic domain, which has a structure similar to all other DNA-methyltransferases, and a smaller DNA-recognition domain. We have investigated this enzyme by random mutagenesis, using error-prone PCR, follo...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/bchm.1998.379.4-5.475
更新日期:1998-04-01 00:00:00
abstract::The sphingolipid sphingosine-1-phosphate (S1P) has various functions in immune cell biology, regulating survival, proliferation, and, most prominently, migration. S1P couples to five G protein-coupled receptors (S1PR1-5) to transduce its effects on immune cell function. Expression of S1PR4 is restricted to immune cell...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2014-0271
更新日期:2015-06-01 00:00:00
abstract::Consumption of green tea is associated with a decrease in cardiovascular mortality. The beneficial health effects of green tea are attributed in part to polyphenols, organic compounds found in tea that lower blood pressure, reduce body fat, decrease LDL cholesterol, and inhibit inflammation. We hypothesized that epiga...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2008.095
更新日期:2008-07-01 00:00:00
abstract::Fibroblast growth factor (FGF) receptor substrate 2 (FRS2) is a membrane-anchored docking protein that has been shown to play an important role in linking FGF, nerve growth factor (NGF) and glial cell-derived neurotrophic factor (GDNF) receptors with the Ras/mitogen-activated protein (MAP) kinase signaling cascade. He...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2003.134
更新日期:2003-08-01 00:00:00
abstract::The cloning of a Ca2+(0)-sensing receptor (CaR) from parathyroid and kidney, coupled with the identification of inherited disorders in Ca2+(0)-sensing resulting from inactivating or activating mutations of this CaR, has provided substantial insights into how Ca2+(0) is regulated at a systemic level. The CaR plays a ce...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:
更新日期:1997-08-01 00:00:00
abstract::The immunoproteasome subunit low molecular weight protein 2 (LMP2) codon 60 polymorphism has been associated with autoimmune diseases. It has also been demonstrated to influence susceptibility to TNF-alpha-induced apoptosis in blood cells and proteasome activity in aged human brain. In the present study, an in silico ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2006.056
更新日期:2006-04-01 00:00:00
abstract::Parvulins belong to the family of peptidyl-prolyl cis/trans isomerases (PPIases) assisting in protein folding and in regulating the function of a broad variety of proteins in all branches of life. The human representatives Pin1 and Par14/17 are directly involved in processes influencing cellular maintenance and cell f...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2017-0137
更新日期:2018-01-26 00:00:00
abstract::The relationship between the structure and activity of meta- and para-hydroxylated monophenols was studied during their tyrosinase-catalysed hydroxylation and the rate-limiting steps of the reaction mechanism were identified. The para-hydroxylated substrates permit us to study the effect of a substituent (R) in the ca...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2000.041
更新日期:2000-04-01 00:00:00
abstract::The influence of the cellular milieu, a complex and crowded solvent, is often neglected when biomolecular structure and function are studied in vitro. To mimic the cellular environment, crowding effects are commonly induced in vitro using artificial crowding agents like Ficoll or dextran. However, it is unclear if suc...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2015-0161
更新日期:2016-01-01 00:00:00
abstract::Abstract The homeostasis and atheroprotective function of bilirubin could be an appealing model to investigate one of the many physiologic functions of the human aryl hydrocarbon receptor (AhR). Several clinical and epidemiological studies have been carried out on key enzymes generating and eliminating bilirubin (heme...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2010.065
更新日期:2010-06-01 00:00:00