Amylin-induced cytotoxicity is associated with activation of caspase-3 and MAP kinases.

abstract：:Kallikrein 6 (KLK6) is a trypsin-like serine peptidase whose relevance in various types of cancers is currently being explored. Previous studies have shown that KLK6 mRNA is upregulated in colon and gastric cancers; however, the regulatory mechanisms and phenotypic consequences of this upregulation are largely unknown...

journal_title：Biological chemistry

authors： Henkhaus RS,Gerner EW,Ignatenko NA

更新日期：2008-06-01 00:00:00

abstract：:The p53 tumour suppressor gene is frequently mutated in human tumours and different tumour-derived mutations have varying effects on cells. The effect of a novel tumour-derived p53 mutation and two recently described mutations from South African breast cancer patients on the growth rate, colony formation, cell cycle a...

journal_title：Biological chemistry

authors： Donninger H,Binder A,Bohm L,Parker MI

更新日期：2008-01-01 00:00:00

abstract：:The periplasmic ligand-binding protein ChoX is part of the ABC transport system ChoVWX that imports choline as a nutrient into the soil bacterium Sinorhizobium meliloti. We have recently reported the crystal structures of ChoX in complex with its ligands choline and acetylcholine and the structure of a fully closed bu...

journal_title：Biological chemistry

authors： Oswald C,Smits SH,Höing M,Bremer E,Schmitt L

更新日期：2009-11-01 00:00:00

abstract：:The thyroglobulin type-1 (Tg-1) domain is a protein module that occurs in a variety of secreted and membrane proteins and is recognised as a potent inhibitor of cysteine peptidases. We present here some properties of the Tg-1 domain of human testican, a modularly organised proteoglycan secreted mainly by brain cells, ...

journal_title：Biological chemistry

authors： Meh P,Pavsic M,Turk V,Baici A,Lenarcic B

更新日期：2005-01-01 00:00:00

abstract：:The kallikrein-kinin and renin-angiotensin (KKS-RAS) systems represent two highly regulated proteolytic systems that are involved in several physiological and pathological processes. Although their protein-protein interactions can be studied using experimental approaches, it is difficult to differentiate between direc...

journal_title：Biological chemistry

authors： Stoka V,Turk V

更新日期：2010-04-01 00:00:00

abstract：:The regulation of eukaryotic gene expression is a complicated process involving the interaction of a large number of transacting factors with specific cis-regulatory elements. DNA methylation plays a role in this scheme by acting in cis to modulate protein-DNA interactions. Several lines of evidence indicate that meth...

journal_title：Biological chemistry

authors： Bergman Y,Mostoslavsky R

更新日期：1998-04-01 00:00:00

abstract：:Dipeptidyl peptidase-IV has been demonstrated to play a role in cancer biology by many authors. Since then, additional proteins possessing similar enzymatic activity have been described and their role in cancerogenesis has been hypothesized. To assess the complexity of these 'Dipeptidyl peptidase-IV activity and/or st...

journal_title：Biological chemistry

authors： Sedo A,Busek P,Scholzová E,Malík R,Vlasicová K,Janácková S,Mares V

更新日期：2004-06-01 00:00:00

abstract：:Small-angle scattering (SAS) of X-rays and neutrons reveals low-resolution structures of biological macromolecules in solution. With the recent experimental and methodological advances, SAS became a unique tool for characterising biological systems. The method covers an extremely broad range of molecule sizes (from a ...

journal_title：Biological chemistry

authors： Svergun DI

更新日期：2010-07-01 00:00:00

abstract：:Two members of the nuclear receptor superfamily, EcR (ecdysteroid receptor protein) and Usp (Ultraspiracle), heterodimerize to form a functional receptor for the steroid hormone 20-hydroxyecdysone and thus enable it to coordinate morphogenetic events during insect metamorphosis. N-terminally His-tagged Usp was overexp...

journal_title：Biological chemistry

authors： Rymarczyk G,Grad I,Rusek A,Oświecimska-Rusin K,Niedziela-Majka A,Kochman M,Ozyhar A

更新日期：2003-01-01 00:00:00

abstract：:Dog pancreas microsomes represent the key components of the established model system for the analysis of protein transport into the mammalian endoplasmic reticulum. More recently, these microsomes were also employed in cell-free systems which address questions related to protein folding and protein degradation in the ...

journal_title：Biological chemistry

authors： Bies C,Guth S,Janoschek K,Nastainczyk W,Volkmer J,Zimmermann R

更新日期：1999-10-01 00:00:00

abstract：:Among the Ras family, Ran is a unique small G protein. It does not have a lipid modification motif at the C-terminus to bind to the membrane, which is often observed within the Ras family. Ran may therefore interact with a wide range of proteins in various intracellular locations. This means that Ran could play many d...

journal_title：Biological chemistry

authors： Nishimoto T

更新日期：2000-05-01 00:00:00

abstract：:The cell, as a molecular system, is often interpreted in terms of complex clockworks, and the design charts of mechanical and electrical engineering are assumed to provide adequate approximations for the description of cellular organization. However, a growing body of experimental evidence obtained through the observa...

journal_title：Biological chemistry

authors： Kurakin A

更新日期：2005-03-01 00:00:00

abstract：:Two new fluorescent labels are presented that are optimized for excitation with He/Ne laser and red diode lasers. Application in FCS and labeling of proteins and oligomers are demonstrated. A strong rise of quantum yield and emission life time upon binding to biomolecules are characteristic features of the dyes. ...

journal_title：Biological chemistry

authors： Czerney P,Lehmann F,Wenzel M,Buschmann V,Dietrich A,Mohr GJ

更新日期：2001-03-01 00:00:00

abstract：:Invadolysin is a novel metalloprotease conserved amongst metazoans that is essential for life in Drosophila. We previously showed that invadolysin was essential for the cell cycle and cell migration, linking to metabolism through a role in lipid storage and interaction with mitochondrial proteins. In this study we dem...

journal_title：Biological chemistry

authors： Chang CW,Abhinav K,Di Cara F,Panagakou I,Vass S,Heck MM

更新日期：2017-03-01 00:00:00

abstract：:The purpose of this work was to study the regulative role of the glutamate receptor found earlier in the brain mitochondria. In the present work a glutamate-dependent signaling system with similar features was detected in mitochondria of the heart. The glutamate-dependent signaling system in the heart mitochondria was...

journal_title：Biological chemistry

authors： Selin AA,Lobysheva NV,Nesterov SV,Skorobogatova YA,Byvshev IM,Pavlik LL,Mikheeva IB,Moshkov DA,Yaguzhinsky LS,Nartsissov YR

更新日期：2016-05-01 00:00:00

abstract：:N-acyl homoserine lactones (AHLs) are quorum sensing (QS) signal molecules used by most Gram-negative pathogenic bacteria. In this article the lactonase activity of the preparations based on hexahistidine-tagged organophosphorus hydrolase (His6-OPH) towards AHLs was studied. Initially, three of the most interesting β-...

journal_title：Biological chemistry

authors： Aslanli A,Lyagin I,Efremenko E

更新日期：2018-07-26 00:00:00

abstract：:The kinin B2 receptor, which is constitutively expressed in a large number of tissues, mediates most of the known effects of bradykinin (BK). Normally undetectable in healthy tissues, the B1 receptor is strongly over-expressed under pathological conditions. BK is an important mediator in renal homeostasis and is mainl...

journal_title：Biological chemistry

authors： Bachvarov D,Bachvarova M,Koumangaye R,Klein J,Pesquero JB,Neau E,Bader M,Schanstra JP,Bascands JL

更新日期：2006-01-01 00:00:00

abstract：:The human copper exporters ATP7A and ATP7B contain domains common to all P-type ATPases as well as class-specific features such as six sequential heavy-metal binding domains (HMBD1-HMBD6) and a type-specific constellation of transmembrane helices. Despite the medical significance of ATP7A and ATP7B related to Menkes a...

journal_title：Biological chemistry

authors： Gourdon P,Sitsel O,Lykkegaard Karlsen J,Birk Møller L,Nissen P

更新日期：2012-04-01 00:00:00

abstract：:Protein disulfide isomerase (PDI) is a protein of the endoplasmic reticulum (ER) that is essential for the unscrambling of nonnative disulfide bonds. Here, we have determined the importance of PDI to both spore germination and vegetative cell division. To vary the concentration of PDI in the ER, we used plasmids that ...

journal_title：Biological chemistry

authors： Laboissière MC,Sturley SL,Raines RT

更新日期：1997-05-01 00:00:00

abstract：:Five phage displayed peptide libraries were screened for binders to C1q, the recognition subunit of the classical complement pathway. Two rounds of panning resulted in the isolation and characterisation of several different phage displayed C1q-binding peptides from all five libraries. Two groups of the characterised p...

journal_title：Biological chemistry

authors： Lauvrak V,Brekke OH,Ihle O,Lindqvist BH

更新日期：1997-12-01 00:00:00

abstract：:The cloning of a Ca2+(0)-sensing receptor (CaR) from parathyroid and kidney, coupled with the identification of inherited disorders in Ca2+(0)-sensing resulting from inactivating or activating mutations of this CaR, has provided substantial insights into how Ca2+(0) is regulated at a systemic level. The CaR plays a ce...

journal_title：Biological chemistry

authors： Chattopadhyay N,Vassilev PM,Brown EM

更新日期：1997-08-01 00:00:00

abstract：:Tissue kallikrein KLK1 and the kallikrein-related peptidases KLK2-15 are a subfamily of serine proteases that have defined or proposed roles in a range of central nervous system (CNS) and non-CNS pathologies. To further understand their potential activity in multiple sclerosis (MS), serum levels of KLK1, 6, 7, 8 and 1...

journal_title：Biological chemistry

authors： Scarisbrick IA,Linbo R,Vandell AG,Keegan M,Blaber SI,Blaber M,Sneve D,Lucchinetti CF,Rodriguez M,Diamandis EP

更新日期：2008-06-01 00:00:00

abstract：:A high-resolution crystallographic structure determination of a protein-ligand complex is generally accepted as the 'gold standard' for structure-based drug design, yet the relationship between structure and affinity is neither obvious nor straightforward. Here we analyze the interactions of a series of serine protein...

journal_title：Biological chemistry

authors： Tziridis A,Rauh D,Neumann P,Kolenko P,Menzel A,Bräuer U,Ursel C,Steinmetzer P,Stürzebecher J,Schweinitz A,Steinmetzer T,Stubbs MT

更新日期：2014-07-01 00:00:00

abstract：:The formation of N-glycosidic linkages of glycoproteins involves the ordered assembly of the common Glc3Man9GlcNAc2 core-oligosaccharide on the lipid carrier dolichyl pyrophosphate. Whereas early mannosylation steps occur on the cytoplasmic side of the endoplasmic reticulum with GDP-Man as donor, the final reactions f...

journal_title：Biological chemistry

authors： Sharma CB,Knauer R,Lehle L

更新日期：2001-02-01 00:00:00

abstract：:This article presents a historical account of the discovery of reversible protein phosphorylation. The process was uncovered in the mid-1950s in a study designed to elucidate the complex hormonal regulation of skeletal muscle glycogen phosphorylase. This enzyme was originally thought to be regulated by AMP now known t...

journal_title：Biological chemistry

authors： Fischer EH

更新日期：2010-02-01 00:00:00

abstract：:In order to identify regulatory elements involved in the hepatocyte specific expression of the enzyme glutamine synthetase [GS (E.C. 6.3.1.2)] we analyzed the first intron of the rat GS gene. A sequence analysis detected clusters of potential transcription factor binding sites in regions that are hypersensitive for DN...

journal_title：Biological chemistry

authors： Gaunitz F,Gaunitz C,Papke M,Gebhardt R

更新日期：1997-01-01 00:00:00

abstract：:Amphetamine ('Speed'), methamphetamine ('Ice') and its congener 3,4-methylenedioxymethamphetamine (MDMA; 'Ecstasy') are illicit drugs abused worldwide for their euphoric and stimulant effects. Despite compelling evidence for chronic MDMA neurotoxicity in animal models, the physiological consequences of such toxicity i...

journal_title：Biological chemistry

authors： Steinkellner T,Freissmuth M,Sitte HH,Montgomery T

更新日期：2011-01-01 00:00:00

abstract：:Structural and functional characterization of integral membrane proteins in a bilayer environment is strongly hampered by the requirement of detergents for solubilization and subsequent purification, as detergents commonly affect their structure and/or activity. Here, we describe a rapid procedure with minimal exposur...

journal_title：Biological chemistry

authors： Shirzad-Wasei N,van Oostrum J,Bovee-Geurts PH,Kusters LJ,Bosman GJ,DeGrip WJ

更新日期：2015-08-01 00:00:00

abstract：:The expression of the cysteine protease cathepsin B is increased in early stages of human breast cancer.To assess the potential role of cathepsin B in premalignant progression of breast epithelial cells, we employed a 3D reconstituted basement membrane overlay culture model of MCF10A human breast epithelial cells and ...

journal_title：Biological chemistry

authors： Mullins SR,Sameni M,Blum G,Bogyo M,Sloane BF,Moin K

更新日期：2012-12-01 00:00:00

abstract：:The main function of the epidermis is to establish a vital multifunctional barrier between the body and its external environment. A defective epidermal barrier is one of the key features of atopic dermatitis (AD), a chronic and relapsing inflammatory skin disorder that affects up to 20% of children and 2-3% of adults ...

journal_title：Biological chemistry

authors： Le Lamer M,Pellerin L,Reynier M,Cau L,Pendaries V,Leprince C,Méchin MC,Serre G,Paul C,Simon M

更新日期：2015-11-01 00:00:00