Abstract:
:The periplasmic ligand-binding protein ChoX is part of the ABC transport system ChoVWX that imports choline as a nutrient into the soil bacterium Sinorhizobium meliloti. We have recently reported the crystal structures of ChoX in complex with its ligands choline and acetylcholine and the structure of a fully closed but substrate-free state of ChoX. This latter structure revealed an architecture of the ligand-binding site that is superimposable to the closed, ligand-bound form of ChoX. We report here the crystal structure of ChoX in an unusual, ligand-free conformation that represents a semi-closed form of ChoX. The analysis revealed a subdomain movement in the N-lobe of ChoX. Comparison with the two well-characterized substrate binding proteins, MBP and HisJ, suggests the presence of a similar subdomain in these proteins.
journal_name
Biol Chemjournal_title
Biological chemistryauthors
Oswald C,Smits SH,Höing M,Bremer E,Schmitt Ldoi
10.1515/BC.2009.113subject
Has Abstractpub_date
2009-11-01 00:00:00pages
1163-70issue
11eissn
1431-6730issn
1437-4315journal_volume
390pub_type
杂志文章abstract::RNAs are key players in life as they connect the genetic code (DNA) with all cellular processes dominated by proteins. They contain a variety of chemical modifications and many RNAs fold into complex structures. Here, we review recent progress in the analysis of RNA modification and structure on the basis of stable is...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2018-0447
更新日期:2019-06-26 00:00:00
abstract::To elucidate the structures and dynamics of membrane proteins, highly advanced biophysical methods have been developed that often require significant resources, both for sample preparation and experimental analyses. For very complex systems, such as membrane transporters, ion channels or G-protein coupled receptors (G...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2018-0473
更新日期:2019-09-25 00:00:00
abstract::The saline extract of Bauhinia bauhinioides dry seeds was shown to inhibit cruzipain, a cysteine proteinase from Trypanosoma cruzi. The inhibitory activity was assigned to a protein with 164 amino acid residues and molecular mass of 18 034 Da that was purified by chromatography on DEAE-Sephadex, trypsin-Sepharose (rem...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2001.103
更新日期:2001-05-01 00:00:00
abstract::Fibroblast growth factor (FGF) receptor substrate 2 (FRS2) is a membrane-anchored docking protein that has been shown to play an important role in linking FGF, nerve growth factor (NGF) and glial cell-derived neurotrophic factor (GDNF) receptors with the Ras/mitogen-activated protein (MAP) kinase signaling cascade. He...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2003.134
更新日期:2003-08-01 00:00:00
abstract::92 kDa and 72 kDa gelatinases, two neutral proteinases exhibiting elastinolytic activity and secreted as zymogens by aortic smooth muscle cells, were shown to bind to insoluble elastin. The active form of each enzyme interacted with substrate more avidly than latent form. Once bound to insoluble elastin, 92 kDa progel...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/bchm.1997.378.3-4.265
更新日期:1997-03-01 00:00:00
abstract::Dendritic cells (DCs) are an important link between innate and adaptive immunity. DCs get activated in inflamed tissues where oxygen tension is usually low, which requires the transcription factor hypoxia inducible factor (HIF)-1 for cellular adaptation. To investigate whether the HIF-1 transcriptional complex plays a...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2012-0320
更新日期:2013-04-01 00:00:00
abstract::Among the Ras family, Ran is a unique small G protein. It does not have a lipid modification motif at the C-terminus to bind to the membrane, which is often observed within the Ras family. Ran may therefore interact with a wide range of proteins in various intracellular locations. This means that Ran could play many d...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2000.052
更新日期:2000-05-01 00:00:00
abstract::Heparan sulfate proteoglycans are obligatory for receptor binding and mitogenic activity of the basic fibroblast growth factor (bFGF). In the present study the influence of undersulfated heparan sulfate on the expression of basic fibroblast growth factor and coronary smooth muscle cell (cSMC) proliferation was investi...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/bchm.1997.378.7.701
更新日期:1997-07-01 00:00:00
abstract::The amyloid precursor protein (APP) and its processing are widely believed to be central for the etiology of Alzheimer's disease (AD) and appear essential for neuronal development and cell homeostasis in mammals. Many studies show the proteolysis of APP by the proteases α-, β- and γ-secretase, functional aspects of th...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2013-0280
更新日期:2014-05-01 00:00:00
abstract::A novel approach to studying the inter- and intrasubunit communication required for the activity of homodimeric proteins is described. It was developed for the restriction endonuclease EcoRV, but should also be useful for other homodimeric enzymes. Two ecorV genes encoding different EcoRV mutants are coexpressed in th...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/bchm3.1996.377.10.625
更新日期:1996-10-01 00:00:00
abstract::In preeclampsia and hemolysis, elevated liver enzymes and low platelet (HELLP) syndrome, impaired trophoblast invasion and excessive fibrin deposition in the placental intervillous space is associated with fetal compromise. However, little information is available whether modulation of placental protease expression--p...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2009.123
更新日期:2009-11-01 00:00:00
abstract::The mechanism of interaction of the lipopolysaccharide (LPS)-binding protein, LBP, with differently composed symmetric and asymmetric planar lipid bilayers was investigated in electrical measurements (membrane current, potential, capacitance). From a change of the inner membrane potential difference, binding of LBP to...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2001.052
更新日期:2001-03-01 00:00:00
abstract::In order to study the role of N-glycans in the ER-associated degradation of unassembled immunoglobulin light (Ig L) chains, we introduced N-glycan acceptor sites into the variable domain of the murine Ig L chain kappaNS1, which is unfolded in unassembled molecules. We investigated the fate of kappaNS1 glycosylated at ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2000.143
更新日期:2000-12-01 00:00:00
abstract::The surface of spherical, nonporous silica nanoparticles (SiO2-NPs) was modified with gadolinium (Gd) complexes, fluorophores, and cell-penetrating peptides to achieve multifunctionality on a single particle. The Gd surface concentrations were 9-16 μmol/g resulting in nanomaterials with high local longitudinal and tra...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2012-0251
更新日期:2013-01-01 00:00:00
abstract::Myocardial zinc dyshomeostasis is associated with caspase-3 activation, ErbB2 degradation and apoptosis during hypoxia/reoxygenation. Zinc pyrithione replenishes intracellular zinc, suppresses caspase-3, augments ErbB2 levels and improves cell survival. We hypothesize that zinc is capable of modulating redox and endop...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2020-0167
更新日期:2020-10-25 00:00:00
abstract::The thyroglobulin type-1 (Tg-1) domain is a protein module that occurs in a variety of secreted and membrane proteins and is recognised as a potent inhibitor of cysteine peptidases. We present here some properties of the Tg-1 domain of human testican, a modularly organised proteoglycan secreted mainly by brain cells, ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2005.010
更新日期:2005-01-01 00:00:00
abstract::Many aspects of the RNA maturation leave traces in RNA sequencing data in the form of deviations from the reference genomic DNA. This includes, in particular, genomically non-encoded nucleotides and chemical modifications. The latter leave their signatures in the form of mismatches and conspicuous patterns of sequenci...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2011.043
更新日期:2011-04-01 00:00:00
abstract::The reverse action of a trypsin-free elastase isolated from porcine pancreas was studied in frozen aqueous systems. Under frozen state conditions, porcine pancreatic elastase was able to catalyse peptide bond formation more effectively than in solution at room temperature. The acceptance of free amino acids as nucleop...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract::Chitinase-like proteins (CLPs) are lectins combining properties of cytokines and growth factors. Human CLPs include YKL-40, YKL-39 and SI-CLP that are secreted by cancer cells, macrophages, neutrophils, synoviocytes, chondrocytes and other cells. The best investigated CLP in cancer is YKL-40. Serum and plasma levels o...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2015-0269
更新日期:2016-03-01 00:00:00
abstract::Maintenance of cellular redox control is pivotal for normal cellular functions and cell fate decisions including cell death. Among the key cellular redox systems in mammals, the glutathione peroxidase (GPX) family of proteins is the largest conferring multifaceted functions and affecting virtually all cellular process...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2020-0286
更新日期:2020-10-22 00:00:00
abstract::TNF-alpha is a major pro-inflammatory cytokine that regulates further cytokine induction, especially of IL-1 and IL-6, in many human diseases including cancer, inflammation and immune disorders. In a search for new inhibitors of inducible TNF-alpha promoter activity and expression, cultures of the imperfect fungus Tri...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2007.066
更新日期:2007-06-01 00:00:00
abstract::In order to enhance and target gene delivery we have previously established a novel method, termed magnetofection, which uses magnetic force acting on gene vectors that are associated with magnetic particles. Here we review the benefits, the mechanism and the potential of the method with regard to overcoming physical ...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2003.082
更新日期:2003-05-01 00:00:00
abstract::There are enzymatic and non-enzymatic mechanisms that generate NO* from nitrite in blood, stomach, saliva, urine and skin. In blood vessels, nitrite-derived NO* can provide protection via compensatory vasodilation during hypoxia, and in various body fluids it may have antibacterial activity. In the skin, nitrite-deriv...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2006.065
更新日期:2006-05-01 00:00:00
abstract::The solute carrier family SLC10 consists of seven members, including the bile acid transporters Na+/taurocholate co-transporting polypeptide (NTCP) and apical sodium-dependent bile acid transporter (ASBT), the steroid sulfate transporter SOAT as well as four orphan carriers (SLC10A3, SLC10A4, SLC10A5 and SLC10A7). Pre...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/hsz-2019-0148
更新日期:2019-09-25 00:00:00
abstract::We have investigated the methylation status of the repeat unit of the human ribosomal RNA genes in lung cancer. Using a Southern blot analysis approach we have determined that the non-transcribed region of these genes was generally heavily methylated, while the transcribed region was not methylated in either tumor or ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.1999.010
更新日期:1999-01-01 00:00:00
abstract::Haptoglobin (Hpt) is known to capture circulating free hemoglobin (Hb) and bind apolipoprotein (Apo) A-I or E. Here, we report that Hb can be tightly bound by most of Hpt molecules (TB-Hpt, 80%), whereas loosely bound by a minor part of them (LB-Hpt, 20%). LB-Hpt amount was significantly increased (over 60%) in patien...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2011.033
更新日期:2011-04-01 00:00:00
abstract::Signalling molecules are produced, degraded, modified and transported throughout the development of higher organisms. Understanding their mode of action implies understanding these dynamics in vivo and in real time. Genetically encoded biosensors are being more and more used as tools to 'follow' signalling molecules a...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/hsz-2018-0310
更新日期:2019-03-26 00:00:00
abstract::Insulin contains three disulfide bonds, one intrachain bond, A6-A11, and two interchain bonds, A7-B7 and A20-B19. Site-directed mutagenesis results (the two cysteine residues of disulfide A7-B7 were replaced by serine) showed that disulfide A7-B7 is crucial to both the structure and activity of insulin. However, chemi...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2004.151
更新日期:2004-12-01 00:00:00
abstract::Plasma prekallikrein (PPK) is synthesised in hepatocytes and secreted into the blood, where it participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. Recently we demonstrated by quantitative RT-PCR that the human PPK gene is transcribed not only in the l...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2007.104
更新日期:2007-09-01 00:00:00
abstract::Bacterial proteases secreted into an infected host may exhibit a wide range of pathogenic potentials. Staphylococci, in particular Staphylococcus aureus, are known to produce several extracellular proteases, including serine-, cysteine- and metalloenzymes. Their insensitivity to most human plasma protease inhibitors a...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2002.116
更新日期:2002-07-01 00:00:00