Structure of cruzipain/cruzain inhibitors isolated from Bauhinia bauhinioides seeds.

abstract：:Interleukin-13 (IL-13) is a T-cell-derived pleiotropic cytokine of particular medical importance because of its critical role in the development of allergic asthma. The effects of IL-13 on its target cells are mediated through a dimeric transmembrane receptor (IL-13R), which shares the IL-4Ralpha subunit with the IL-4...

journal_title：Biological chemistry

authors： Knackmuss S,Krause S,Engel K,Reusch U,Virchow JC,Mueller T,Kraich M,Little M,Luttmann W,Friedrich K

更新日期：2007-03-01 00:00:00

abstract：:The 15 members of the kallikrein-related serine peptidase (KLK) family have diverse tissue-specific expression profiles and roles in a range of cellular processes, including proliferation, migration, invasion, differentiation, inflammation and angiogenesis that are required in both normal physiology as well as patholo...

journal_title：Biological chemistry

authors： Dong Y,Harrington BS,Adams MN,Wortmann A,Stephenson SA,Lisle J,Herington A,Hooper JD,Clements JA

更新日期：2014-09-01 00:00:00

abstract：:The ATP-binding cassette (ABC) transporter genes represent the largest family of transporters and these genes are abundant in the genome of all vertebrates. Through analysis of the genome sequence databases we have characterized the full complement of ABC genes from several mammals and other vertebrates. Multiple gene...

journal_title：Biological chemistry

authors： Moitra K,Dean M

更新日期：2011-01-01 00:00:00

abstract：:Arginine deiminase (ADI) is a potential antitumor agent for the arginine deprivation treatment of L-arginine auxotrophic tumors. The optimum pH of ADI varies significantly, yet little is known about the origin of this variety. Here, Pseudomonas aeruginosa ADI (PaADI), an enzyme that functions only at acidic pH, was ut...

journal_title：Biological chemistry

authors： Ding H,Liu H,Yin Y,Ding Y,Jia Y,Chen Q,Zou G,Zheng Z

更新日期：2012-09-01 00:00:00

abstract：:A deletion mutant (V314) of the calcineurin A subunit was constructed using site-directed mutagenesis. Its phosphatase activity and function were then characterized. The V314 deletion significantly altered the phosphatase activity, which was more than ten times higher than that of wild-type calcineurin, the calcineuri...

journal_title：Biological chemistry

authors： Yan L,Wei Q

更新日期：1999-11-01 00:00:00

abstract：:There is increasing evidence that apoptosis and necrosis represent only two of several possible ways for cells to die. These two types of demise can occur simultaneously in tissues or cell cultures exposed to the same stimulus, and often local metabolic conditions and the intensity of the same initial insult decide th...

journal_title：Biological chemistry

authors： Nicotera P,Leist M,Single B,Volbracht C

更新日期：1999-09-01 00:00:00

abstract：:Based on studies presented here and other published experiments performed with surviving tissue preparations, with transfected cells and with cells that constitutively express the human angiotensin I converting enzyme ACE and B2 receptors, we concluded the following: ACE inhibitors and other endogenous peptides that r...

journal_title：Biological chemistry

authors： Erdös EG,Marcic BM

更新日期：2001-01-01 00:00:00

abstract：:The cell, as a molecular system, is often interpreted in terms of complex clockworks, and the design charts of mechanical and electrical engineering are assumed to provide adequate approximations for the description of cellular organization. However, a growing body of experimental evidence obtained through the observa...

journal_title：Biological chemistry

authors： Kurakin A

更新日期：2005-03-01 00:00:00

abstract：:Calpain is a calcium-activated non lysosomal neutral thiol protease (EC 3.4.22.17) present in a wide variety of eukaryotic cells. Calpain is usually present as an inactive form and is activated by calcium ions and phospholipids. The ability of calpain to alter, by limited proteolysis, the activity or function of numer...

journal_title：Biological chemistry

authors： Yajima Y,Kawashima S

更新日期：2002-05-01 00:00:00

abstract：:Animal cells release traces of material onto glass or silicon surfaces during adhesion and migration. This little studied phenomenon is a widespread and normal concomitant of cell migration. The paper introduces the study of such material. The traces can be visualised by different microscopic techniques (e.g. TIRF, IR...

journal_title：Biological chemistry

authors： Fuhr G,Richter E,Zimmermann H,Hitzler H,Niehus H,Hagedorn R

更新日期：1998-08-01 00:00:00

abstract：:The reverse action of a trypsin-free elastase isolated from porcine pancreas was studied in frozen aqueous systems. Under frozen state conditions, porcine pancreatic elastase was able to catalyse peptide bond formation more effectively than in solution at room temperature. The acceptance of free amino acids as nucleop...

journal_title：Biological chemistry

authors： Haensler M,Wehofsky N,Gerisch S,Wissmann JD,Jakubke HD

更新日期：1998-01-01 00:00:00

abstract：:Meprins are zinc-endopeptidases of the astacin family, which are expressed as membrane-bound or secreted forms in renal and intestinal brush-border membranes of mouse, rat and man. There are two types of meprin subunits, alpha and beta, which form disulfide-bonded homo- and heterodimers; further oligomerization is med...

journal_title：Biological chemistry

authors： Becker C,Kruse MN,Slotty KA,Köhler D,Harris JR,Rösmann S,Sterchi EE,Stöcker W

更新日期：2003-05-01 00:00:00

abstract：:The neurobiological mechanisms of obesity-induced peripheral neuropathy are poorly understood. We evaluated the role of Sigma-1 receptor (Sig-1R) and NMDA receptor (NMDARs) in the spinal cord in peripheral neuropathy using an animal model of high fat diet-induced diabetes. We examined the expression of Sig-1R and NMDA...

journal_title：Biological chemistry

authors： Song T,Zhao J,Ma X,Zhang Z,Jiang B,Yang Y

更新日期：2017-09-26 00:00:00

abstract：:As the outermost barrier of the body, the skin is directly and frequently exposed to a prooxidative environment, including solar UVA and UVB radiation, and air pollution. Ozone, a major air pollutant, possesses a strong oxidizing potential and therefore is possibly one of the most reactive chemicals the skin ever meet...

journal_title：Biological chemistry

authors： Thiele JJ,Podda M,Packer L

更新日期：1997-11-01 00:00:00

abstract：:Haptoglobin (Hpt) is known to capture circulating free hemoglobin (Hb) and bind apolipoprotein (Apo) A-I or E. Here, we report that Hb can be tightly bound by most of Hpt molecules (TB-Hpt, 80%), whereas loosely bound by a minor part of them (LB-Hpt, 20%). LB-Hpt amount was significantly increased (over 60%) in patien...

journal_title：Biological chemistry

authors： Spagnuolo MS,Cigliano L,Maresca B,Pugliese CR,Abrescia P

更新日期：2011-04-01 00:00:00

abstract：:Here we report a detailed analysis of magnesium (Mg²+) ion effects on furin hydrolysis of fluorescent resonance energy transfer decapeptide substrates derived from canonical R-X-K/R-R furin cleavage motifs within certain viral envelope glycoproteins and eukaryotic proproteins. Using virus-derived sequences a selective...

journal_title：Biological chemistry

authors： Izidoro MA,Assis DM,Oliveira V,Santos JA,Juliano MA,Lindberg I,Juliano L

更新日期：2010-09-01 00:00:00

abstract：:Rasal is a modular multi-domain protein of the GTPase-activating protein 1 (GAP1) family; its four known members, GAP1m, Rasal, GAP1IP4BP and Capri, have a Ras GTPase-activating domain (RasGAP). This domain supports the intrinsically slow GTPase activity of Ras by actively participating in the catalytic reaction. In t...

journal_title：Biological chemistry

authors： Cuellar J,Valpuesta JM,Wittinghofer A,Sot B

更新日期：2017-12-20 00:00:00

abstract：:Abstract Hairpin ribozymes occur naturally only in the satellite RNAs of tobacco ringspot virus (TRsV), chicory yellow mottle virus (CYMoV) and arabis mosaic virus (ArMV). The catalytic centre of the predominantly studied sTRsV hairpin ribozyme, and of sArMV is organised around a four-way helical junction. We show her...

journal_title：Biological chemistry

authors： Bajaj P,Steger G,Hammann C

更新日期：2011-07-01 00:00:00

abstract：:The mechanism of interaction of the lipopolysaccharide (LPS)-binding protein, LBP, with differently composed symmetric and asymmetric planar lipid bilayers was investigated in electrical measurements (membrane current, potential, capacitance). From a change of the inner membrane potential difference, binding of LBP to...

journal_title：Biological chemistry

authors： Gutsmann T,Haberer N,Carroll SF,Seydel U,Wiese A

更新日期：2001-03-01 00:00:00

abstract：:We present a novel strategy, termed CISTEM, which allows direct in vivo screening of polypeptides displayed on the surface of E. coli cells by a combination of ligand-mediated protection and phage-mediated selection. The effectiveness of this new approach was demonstrated by displaying the T7.tag on the surface of E. ...

journal_title：Biological chemistry

authors： Camaj P,Hirsh AE,Schmidt W,Meinke A,von Gabain A

更新日期：2001-12-01 00:00:00

abstract：:G-protein coupled receptor 119 (GPR119) is emerging as a potential target for the treatment of type 2 diabetes with beneficial effects on glucose homeostasis. This study assessed the insulin-secreting properties of various GPR119 agonists and the distribution of GPR119 in pancreatic islets. Endogenous ligands [oleoyle...

journal_title：Biological chemistry

authors： Moran BM,Abdel-Wahab YH,Flatt PR,McKillop AM

更新日期：2014-04-01 00:00:00

abstract：:The vast majority of malignancies detected in renal parenchyma are diagnosed as renal cell carcinoma (RCC), whose subtype discrimination and determination of prognosis may contribute to the selection of the adequate therapy. Recently, a new class of small non-coding RNAs, known as microRNAs, has proven to be among the...

journal_title：Biological chemistry

authors： Papadopoulos EI,Petraki C,Gregorakis A,Fragoulis EG,Scorilas A

更新日期：2016-06-01 00:00:00

abstract：:Dog pancreas microsomes represent the key components of the established model system for the analysis of protein transport into the mammalian endoplasmic reticulum. More recently, these microsomes were also employed in cell-free systems which address questions related to protein folding and protein degradation in the ...

journal_title：Biological chemistry

authors： Bies C,Guth S,Janoschek K,Nastainczyk W,Volkmer J,Zimmermann R

更新日期：1999-10-01 00:00:00

abstract：:Historically considered as accessory cells to neurons, there is an increasing interest in the role of astrocytes in normal and pathological conditions. Astrocytes are involved in neurotransmitter recycling, antioxidant supply, ion buffering and neuroinflammation, i.e. a lot of the same pathways that go astray in Alzhe...

journal_title：Biological chemistry

authors： Zulfiqar S,Garg P,Nieweg K

更新日期：2019-08-27 00:00:00

abstract：:Few therapeutic options are offered to treat inflammation and alveolar wall destruction in emphysema. The effect of recombinant human pre-elafin, an elastase inhibitor, was evaluated in porcine pancreatic elastase (PPE)-induced emphysema in C57BL/6 mice. In a first protocol, mice received a single instillation of pre-...

journal_title：Biological chemistry

authors： Janelle MF,Doucet A,Bouchard D,Bourbonnais Y,Tremblay GM

更新日期：2006-07-01 00:00:00

abstract：:The stress-activated protein kinase p38/SAPK2 is known to regulate the activity of transcription factors and to control expression of several genes at the transcriptional or post-transcriptional level. In order to identify genes whose expression is under the control of p38/SAPK2 activity, we have compared the mRNA lev...

journal_title：Biological chemistry

authors： Rolli-Derkinderen M,Gaestel M

更新日期：2000-03-01 00:00:00

abstract：:The formation of N-glycosidic linkages of glycoproteins involves the ordered assembly of the common Glc3Man9GlcNAc2 core-oligosaccharide on the lipid carrier dolichyl pyrophosphate. Whereas early mannosylation steps occur on the cytoplasmic side of the endoplasmic reticulum with GDP-Man as donor, the final reactions f...

journal_title：Biological chemistry

authors： Sharma CB,Knauer R,Lehle L

更新日期：2001-02-01 00:00:00

abstract：:Two new fluorescent labels are presented that are optimized for excitation with He/Ne laser and red diode lasers. Application in FCS and labeling of proteins and oligomers are demonstrated. A strong rise of quantum yield and emission life time upon binding to biomolecules are characteristic features of the dyes. ...

journal_title：Biological chemistry

authors： Czerney P,Lehmann F,Wenzel M,Buschmann V,Dietrich A,Mohr GJ

更新日期：2001-03-01 00:00:00

abstract：:As a crucial protein, the herpes simplex virus 1 (HSV-1) UL2 protein has been shown to take part in various stages of viral infection, nonetheless, its exact subcellular localization and transport molecular determinants are not well known thus far. In the present study, by using live cells fluorescent microscopy assay...

journal_title：Biological chemistry

authors： Cai M,Huang Z,Liao Z,Chen T,Wang P,Jiang S,Chen D,Peng T,Bian Y,Hong G,Yang H,Zeng Z,Li X,Li M

更新日期：2017-04-01 00:00:00

abstract：:The immunoproteasome subunit low molecular weight protein 2 (LMP2) codon 60 polymorphism has been associated with autoimmune diseases. It has also been demonstrated to influence susceptibility to TNF-alpha-induced apoptosis in blood cells and proteasome activity in aged human brain. In the present study, an in silico ...

journal_title：Biological chemistry

authors： Mishto M,Santoro A,Bellavista E,Sessions R,Textoris-Taube K,Dal Piaz F,Carrard G,Forti K,Salvioli S,Friguet B,Kloetzel PM,Rivett AJ,Franceschi C

更新日期：2006-04-01 00:00:00