Abstract:
:Haptoglobin (Hpt) is known to capture circulating free hemoglobin (Hb) and bind apolipoprotein (Apo) A-I or E. Here, we report that Hb can be tightly bound by most of Hpt molecules (TB-Hpt, 80%), whereas loosely bound by a minor part of them (LB-Hpt, 20%). LB-Hpt amount was significantly increased (over 60%) in patients with acute coronary syndrome. LB-Hpt bound ApoA-I and ApoE less efficiently than TB-Hpt (8- and 4-fold less, respectively) and did not affect their activity of stimulating the enzyme lecithin-cholesterol acyltransferase. LB-Hpt and TB-Hpt displayed comparable levels of nitrotyrosine residues, but differences in glycan chains. Changes in LB-Hpt level might be associated with changes in Hpt functions.
journal_name
Biol Chemjournal_title
Biological chemistryauthors
Spagnuolo MS,Cigliano L,Maresca B,Pugliese CR,Abrescia Pdoi
10.1515/BC.2011.033subject
Has Abstractpub_date
2011-04-01 00:00:00pages
371-6issue
4eissn
1431-6730issn
1437-4315journal_volume
392pub_type
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