Nervous system pathology: the fibrin perspective.

abstract：:In bacteria, UGA stop codons can be recoded to direct the incorporation of selenocysteine into proteins on the ribosome. Recoding requires a selenocysteine incorporation sequence (SECIS) downstream of the UGA codon, a specialized translation factor SelB, and the non-canonical Sec-tRNASec, which is formed from Ser-tRNA...

journal_title：Biological chemistry

authors： Fischer N,Paleskava A,Gromadski KB,Konevega AL,Wahl MC,Stark H,Rodnina MV

更新日期：2007-10-01 00:00:00

abstract：:Plasma kallikrein plays a role in coagulation, fibrinolysis and inflammation. Cathepsins B and L participate in (patho)physiological processes such as peptide antigen processing, tissue remodeling events, protein turnover in cells, hormone processing and tumor invasion. The present work analyzes the processing of prek...

journal_title：Biological chemistry

authors： Barros NM,Puzer L,Tersariol IL,Oliva ML,Sampaio CA,Carmona AK,Motta G

更新日期：2004-11-01 00:00:00

abstract：:The surface of spherical, nonporous silica nanoparticles (SiO2-NPs) was modified with gadolinium (Gd) complexes, fluorophores, and cell-penetrating peptides to achieve multifunctionality on a single particle. The Gd surface concentrations were 9-16 μmol/g resulting in nanomaterials with high local longitudinal and tra...

journal_title：Biological chemistry

authors： Joshi R,Feldmann V,Koestner W,Detje C,Gottschalk S,Mayer HA,Sauer MG,Engelmann J

更新日期：2013-01-01 00:00:00

abstract：:Rasal is a modular multi-domain protein of the GTPase-activating protein 1 (GAP1) family; its four known members, GAP1m, Rasal, GAP1IP4BP and Capri, have a Ras GTPase-activating domain (RasGAP). This domain supports the intrinsically slow GTPase activity of Ras by actively participating in the catalytic reaction. In t...

journal_title：Biological chemistry

authors： Cuellar J,Valpuesta JM,Wittinghofer A,Sot B

更新日期：2017-12-20 00:00:00

abstract：:To elucidate the structures and dynamics of membrane proteins, highly advanced biophysical methods have been developed that often require significant resources, both for sample preparation and experimental analyses. For very complex systems, such as membrane transporters, ion channels or G-protein coupled receptors (G...

journal_title：Biological chemistry

authors： Rose-Sperling D,Tran MA,Lauth LM,Goretzki B,Hellmich UA

更新日期：2019-09-25 00:00:00

abstract：:Two current hypotheses of O2 sensing in the carotid body (CB) chemoreceptors suggest participation of oxygen reactive (ROS) species, but they are mechanistically opposed. One postulates that hypoxia decreases ROS levels; the other that hypoxia increases them. Yet, both propose that the ensuing alteration in the cellul...

journal_title：Biological chemistry

authors： Gonzalez C,Sanz-Alyayate G,Agapito MT,Obeso A

更新日期：2004-03-01 00:00:00

abstract：:Invadolysin is a novel metalloprotease conserved amongst metazoans that is essential for life in Drosophila. We previously showed that invadolysin was essential for the cell cycle and cell migration, linking to metabolism through a role in lipid storage and interaction with mitochondrial proteins. In this study we dem...

journal_title：Biological chemistry

authors： Chang CW,Abhinav K,Di Cara F,Panagakou I,Vass S,Heck MM

更新日期：2017-03-01 00:00:00

abstract：:Human inositol phosphate multikinase (IPMK) is a multifunctional protein in cellular signal transduction, namely, a multispecific inositol phosphate kinase, phosphatidylinositol 3-kinase, and a scaffold within the mTOR-raptor complex. To fulfill these nuclear and cytoplasmic functions, intracellular targeting of IPMK ...

journal_title：Biological chemistry

authors： Meyer R,Nalaskowski MM,Ehm P,Schröder C,Naj X,Brehm MA,Mayr GW

更新日期：2012-03-01 00:00:00

abstract：:The three isoforms of human Ins(1,4,5)P3 3-kinase (IP3K) show remarkable differences in their intracellular targeting. Whereas predominant targeting to the cytoskeleton and endoplasmic reticulum has been shown for IP3K-A and IP3K-B, rat IP3K-C shuttles actively between the nucleus and cytoplasm. In the present study w...

journal_title：Biological chemistry

authors： Nalaskowski MM,Windhorst S,Stockebrand MC,Mayr GW

更新日期：2006-05-01 00:00:00

abstract：:A deletion mutant (V314) of the calcineurin A subunit was constructed using site-directed mutagenesis. Its phosphatase activity and function were then characterized. The V314 deletion significantly altered the phosphatase activity, which was more than ten times higher than that of wild-type calcineurin, the calcineuri...

journal_title：Biological chemistry

authors： Yan L,Wei Q

更新日期：1999-11-01 00:00:00

abstract：:Abstract Few genes are known to be involved in renal cell carcinoma (RCC) development and progression. The cell-specific transcription factor hepatocyte nuclear factor 4 alpha (HNF4 alpha) is down-regulated in RCC and we have shown that HNF4 alpha inhibits cell proliferation in the embryonic kidney cell line HEK293. T...

journal_title：Biological chemistry

authors： Grigo K,Wirsing A,Lucas B,Klein-Hitpass L,Ryffel GU

更新日期：2008-02-01 00:00:00

abstract：:The formation of N-glycosidic linkages of glycoproteins involves the ordered assembly of the common Glc3Man9GlcNAc2 core-oligosaccharide on the lipid carrier dolichyl pyrophosphate. Whereas early mannosylation steps occur on the cytoplasmic side of the endoplasmic reticulum with GDP-Man as donor, the final reactions f...

journal_title：Biological chemistry

authors： Sharma CB,Knauer R,Lehle L

更新日期：2001-02-01 00:00:00

abstract：:This article presents a historical account of the discovery of reversible protein phosphorylation. The process was uncovered in the mid-1950s in a study designed to elucidate the complex hormonal regulation of skeletal muscle glycogen phosphorylase. This enzyme was originally thought to be regulated by AMP now known t...

journal_title：Biological chemistry

authors： Fischer EH

更新日期：2010-02-01 00:00:00

abstract：:The activation of progelatinase A to gelatinase A requires cleavage of an asparaginyl bond to form the N-terminus of the mature enzyme. We have asked whether the activation can be mediated by legumain, the recently discovered lysosomal cysteine proteinase that is specific for hydrolysis of asparaginyl bonds. Addition ...

journal_title：Biological chemistry

authors： Chen JM,Fortunato M,Stevens RA,Barrett AJ

更新日期：2001-05-01 00:00:00

abstract：:The solute carrier family SLC10 consists of seven members, including the bile acid transporters Na+/taurocholate co-transporting polypeptide (NTCP) and apical sodium-dependent bile acid transporter (ASBT), the steroid sulfate transporter SOAT as well as four orphan carriers (SLC10A3, SLC10A4, SLC10A5 and SLC10A7). Pre...

journal_title：Biological chemistry

authors： Noppes S,Müller SF,Bennien J,Holtemeyer M,Palatini M,Leidolf R,Alber J,Geyer J

更新日期：2019-09-25 00:00:00

abstract：:Recombinant expression of actin in bacteria results in non-native species that aggregate into inclusion bodies. Actin is a folding substrate of TRiC, the chaperonin of the eukaryotic cytosol. By employing bacterial in vitro translation lysates supplemented with purified chaperones, we have found that TRiC is the only ...

journal_title：Biological chemistry

authors： Stemp MJ,Guha S,Hartl FU,Barral JM

更新日期：2005-08-01 00:00:00

abstract：:The neurobiological mechanisms of obesity-induced peripheral neuropathy are poorly understood. We evaluated the role of Sigma-1 receptor (Sig-1R) and NMDA receptor (NMDARs) in the spinal cord in peripheral neuropathy using an animal model of high fat diet-induced diabetes. We examined the expression of Sig-1R and NMDA...

journal_title：Biological chemistry

authors： Song T,Zhao J,Ma X,Zhang Z,Jiang B,Yang Y

更新日期：2017-09-26 00:00:00

abstract：:To facilitate evolutionary and forensic studies of DNA polymorphisms on the Y chromosome, we devised a multiplex amplification procedure for short-tandem-repeat (STR) loci. Four tetranucleotide STR loci (DYS19, DYS390, DYS391, and DYS393) were simultaneously amplified with FAM-labeled primers and genotypes were determ...

journal_title：Biological chemistry

authors： Redd AJ,Clifford SL,Stoneking M

更新日期：1997-08-01 00:00:00

abstract：:N.BstNBI is a unique restriction endonuclease isolated from Bacillus stearothermophilus. We have characterized the recognition sequence and the cleavage site of N.BstNBI. Mapping of cleavage sites of N.BstNBI showed that it recognizes an asymmetric sequence, 5' GAGTC 3', and cleaves only on the top strand 4 base pairs...

journal_title：Biological chemistry

authors： Morgan RD,Calvet C,Demeter M,Agra R,Kong H

更新日期：2000-11-01 00:00:00

abstract：:Human septins comprise a family of 13 genes that encode for >30 protein isoforms with ubiquitous and tissue-specific expressions. Septins are GTP-binding proteins that assemble into higher-order oligomers and filamentous polymers, which associate with cell membranes and the cytoskeleton. In the last decade, much progr...

journal_title：Biological chemistry

authors： Dolat L,Hu Q,Spiliotis ET

更新日期：2014-02-01 00:00:00

abstract：:Bacterial infections often upregulate the plasma kinin-forming cascade of the host (the 'contact system') which is triggered by adsorption of high molecular mass kininogen (HK), coagulation factor XII (FXII) and prekallikrein (pHPK) on the host or pathogen cell surfaces. A possible activation of the contact system upo...

journal_title：Biological chemistry

authors： Karkowska-Kuleta J,Kozik A,Rapala-Kozik M

更新日期：2010-01-01 00:00:00

abstract：:Components of kinin-forming systems operating at inflammatory sites are likely to interact with elastase that is released by recruited neutrophils and may, at least temporarily, constitute the major proteolytic activity present at these sites. The aim of this work was to determine the effect of kininogen degradation b...

journal_title：Biological chemistry

authors： Dulinski R,Suder P,Guevara-Lora I,Rapała-Kozik M,Potempa J,Silberring J,Imamura T,Travis J,Kozik A

更新日期：2003-06-01 00:00:00

abstract：:The lysosomal cysteine proteinase cathepsin B has been implicated in the progression of various human tumors including ovarian cancer. Included in this study were 63 patients with epithelial ovarian carcinoma. Follow-up information (median follow-up period 7 years) was available for all patients, among whom 42 (66.7%)...

journal_title：Biological chemistry

authors： Scorilas A,Fotiou S,Tsiambas E,Yotis J,Kotsiandri F,Sameni M,Sloane BF,Talieri M

更新日期：2002-07-01 00:00:00

abstract：:The Sialyl-Tn antigen (Sialyl alpha-Ser/Thr) is expressed as a cancer-associated antigen on the surface of cancer cells. Its presence is associated with a poor prognosis in patients with colorectal and other cancers. We previously reported that Sialyl-Tn expression in LSC human colon cancer cells could be explained by...

journal_title：Biological chemistry

authors： Brockhausen I,Yang J,Lehotay M,Ogata S,Itzkowitz S

更新日期：2001-02-01 00:00:00

abstract：:Nitrogen dioxide (*NO2) is an oxidizing free radical which can initiate a variety of destructive pathways in living systems, and several diseases are suspected to be connected with both exogenously and endogenously formed *NO2. Peroxynitrite (ONOO-/ONOOH) is believed to be an important endogenous source of *NO2 radica...

journal_title：Biological chemistry

authors： Kirsch M,Korth HG,Sustmann R,de Groot H

更新日期：2002-03-01 00:00:00

abstract：:DNA-based immunization has been shown to induce protective immunity against several microbial pathogens including HIV-1. Several routes of DNA vaccination have been exploited. However, the properties of the immune responses seem to differ with the different routes used for DNA delivery, ultimately affecting the outcom...

journal_title：Biological chemistry

authors： Asakura Y,Lundholm P,Kjerrström A,Benthin R,Lucht E,Fukushima J,Schwartz S,Okuda K,Wahren B,Hinkula J

更新日期：1999-03-01 00:00:00

abstract：:Tissue kallikrein KLK1 and the kallikrein-related peptidases KLK2-15 are a subfamily of serine proteases that have defined or proposed roles in a range of central nervous system (CNS) and non-CNS pathologies. To further understand their potential activity in multiple sclerosis (MS), serum levels of KLK1, 6, 7, 8 and 1...

journal_title：Biological chemistry

authors： Scarisbrick IA,Linbo R,Vandell AG,Keegan M,Blaber SI,Blaber M,Sneve D,Lucchinetti CF,Rodriguez M,Diamandis EP

更新日期：2008-06-01 00:00:00

abstract：:The non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase catalyses the irreversible reaction of glyceraldehyde-3-phosphate to 3-phosphoglycerate by the reduction of NADP to NADPH. This is in contrast to the extensively analysed phosphorylating glyceraldehyde-3-phosphate dehydrogenases which catalyse the revers...

journal_title：Biological chemistry

authors： Habenicht A

更新日期：1997-12-01 00:00:00

abstract：:Cathepsin E (CE) is an intracellular aspartic proteinase implicated in various physiological and pathological processes, yet its actual roles in vivo remain elusive. To assess the physiological significance of CE expression in tumor cells, human CE was stably expressed in human prostate carcinoma ALVA101 cells express...

journal_title：Biological chemistry

authors： Shin M,Kadowaki T,Iwata J,Kawakubo T,Yamaguchi N,Takii R,Tsukuba T,Yamamoto K

更新日期：2007-11-01 00:00:00

abstract：:The bifunctional protein DCoH (dimerization cofactor of HNF-1) is a transcriptional coactivator of homeodomain proteins and a cytoplasmic enzyme. Here we report on the isolation, nucleotide sequence and genomic structure of the chicken and rat DCoH genes. They have an unusual first exon coding for a single amino acid....

journal_title：Biological chemistry

authors： Bossow S,Riepl S,Igo-Kemenes T

更新日期：1998-11-01 00:00:00