Protective activity of aromatic amines and imines against oxidative nerve cell death.

abstract：:A large number of prostate cancer (PCa) patients receive treatment without significant benefits, strengthening the need for accurate prognosis, which can be supported by the study of miRNAs. In silico specificity analysis was performed for the identification of miRNAs able to regulate KLK2 and KLK4 expression. Total R...

journal_title：Biological chemistry

authors： Avgeris M,Stravodimos K,Scorilas A

更新日期：2014-09-01 00:00:00

abstract：:Early diagnostics at the preclinical stage of Alzheimer's disease is of utmost importance for drug development in clinical trials and prognostic guidance. Since soluble Aβ oligomers are considered to play a crucial role in the disease pathogenesis, several methods aim to quantify Aβ oligomers in body fluids such as ce...

journal_title：Biological chemistry

authors： Kravchenko K,Kulawik A,Hülsemann M,Kühbach K,Zafiu C,Herrmann Y,Linnartz C,Peters L,Bujnicki T,Willbold J,Bannach O,Willbold D

更新日期：2017-04-01 00:00:00

abstract：:Components of kinin-forming systems operating at inflammatory sites are likely to interact with elastase that is released by recruited neutrophils and may, at least temporarily, constitute the major proteolytic activity present at these sites. The aim of this work was to determine the effect of kininogen degradation b...

journal_title：Biological chemistry

authors： Dulinski R,Suder P,Guevara-Lora I,Rapała-Kozik M,Potempa J,Silberring J,Imamura T,Travis J,Kozik A

更新日期：2003-06-01 00:00:00

abstract：:Based on its monophenolic structure and given its pharmacological and toxicological importance, the ability of tyrosinase to oxidize acetaminophen was studied for the first time. Progress curves showed a transient phase characteristic of the monophenolase activity of tyrosinase prior to attaining the steady-state. The...

journal_title：Biological chemistry

authors： Valero E,Varón R,García-Carmona F

更新日期：2002-12-01 00:00:00

abstract：:The sphingolipid sphingosine-1-phosphate (S1P) has various functions in immune cell biology, regulating survival, proliferation, and, most prominently, migration. S1P couples to five G protein-coupled receptors (S1PR1-5) to transduce its effects on immune cell function. Expression of S1PR4 is restricted to immune cell...

journal_title：Biological chemistry

authors： Dillmann C,Mora J,Olesch C,Brüne B,Weigert A

更新日期：2015-06-01 00:00:00

abstract：:The lysosomal cysteine proteinase cathepsin B has been implicated in the progression of various human tumors including ovarian cancer. Included in this study were 63 patients with epithelial ovarian carcinoma. Follow-up information (median follow-up period 7 years) was available for all patients, among whom 42 (66.7%)...

journal_title：Biological chemistry

authors： Scorilas A,Fotiou S,Tsiambas E,Yotis J,Kotsiandri F,Sameni M,Sloane BF,Talieri M

更新日期：2002-07-01 00:00:00

abstract：:Human septins comprise a family of 13 genes that encode for >30 protein isoforms with ubiquitous and tissue-specific expressions. Septins are GTP-binding proteins that assemble into higher-order oligomers and filamentous polymers, which associate with cell membranes and the cytoskeleton. In the last decade, much progr...

journal_title：Biological chemistry

authors： Dolat L,Hu Q,Spiliotis ET

更新日期：2014-02-01 00:00:00

abstract：:DEAD-box proteins disrupt or remodel RNA and protein/RNA complexes at the expense of ATP. The catalytic core is composed of two flexibly connected RecA-like domains. The N-terminal domain contains most of the motifs involved in nucleotide binding and serves as a minimalistic model for helicase/nucleotide interactions....

journal_title：Biological chemistry

authors： Strohmeier J,Hertel I,Diederichsen U,Rudolph MG,Klostermeier D

更新日期：2011-04-01 00:00:00

abstract：:Acid ceramidase (N-acylsphingosine deacylase, EC 3.5.1.23; AC) is the lipid hydrolase responsible for the degradation of ceramide into sphingosine and free fatty acids within lysosomes. The enzymatic activity was first identified over four decades ago and is deficient in two rare inherited disorders, Farber lipogranul...

journal_title：Biological chemistry

authors： Frohbergh M,He X,Schuchman EH

更新日期：2015-06-01 00:00:00

abstract：:Human inositol phosphate multikinase (IPMK) is a multifunctional protein in cellular signal transduction, namely, a multispecific inositol phosphate kinase, phosphatidylinositol 3-kinase, and a scaffold within the mTOR-raptor complex. To fulfill these nuclear and cytoplasmic functions, intracellular targeting of IPMK ...

journal_title：Biological chemistry

authors： Meyer R,Nalaskowski MM,Ehm P,Schröder C,Naj X,Brehm MA,Mayr GW

更新日期：2012-03-01 00:00:00

abstract：:To elucidate the structures and dynamics of membrane proteins, highly advanced biophysical methods have been developed that often require significant resources, both for sample preparation and experimental analyses. For very complex systems, such as membrane transporters, ion channels or G-protein coupled receptors (G...

journal_title：Biological chemistry

authors： Rose-Sperling D,Tran MA,Lauth LM,Goretzki B,Hellmich UA

更新日期：2019-09-25 00:00:00

abstract：:This review addresses many of the chemical aspects of nitrosative stress mediated by N2O3. From a cellular perspective, N2O3 and the resulting reactive nitrogen oxide species target specific motifs such as thiols, lysine active sites, and zinc fingers and is dependant upon both the rates of production as well as consu...

journal_title：Biological chemistry

authors： Ridnour LA,Thomas DD,Mancardi D,Espey MG,Miranda KM,Paolocci N,Feelisch M,Fukuto J,Wink DA

更新日期：2004-01-01 00:00:00

abstract：:The surface topography of red blood cells (RBCs) was investigated under near-physiological conditions using atomic force microscopy (AFM). An immobilization protocol was established where RBCs are coupled via molecular bonds of the membrane glycoproteins to wheat germ agglutinin (WGA), which is covalently and flexibly...

journal_title：Biological chemistry

authors： Kamruzzahan AS,Kienberger F,Stroh CM,Berg J,Huss R,Ebner A,Zhu R,Rankl C,Gruber HJ,Hinterdorfer P

更新日期：2004-10-01 00:00:00

abstract：:Five moeA mutants were generated by replacing some conserved amino acids of MoeA by site-directed mutagenesis. The mutants were assayed for the ability to restore in vivo nitrate reductase activity of the moeA mutant Escherichia coli JRG97 and in vitro Neurospora crassa nit-1 nitrate reductase activity. The replacemen...

journal_title：Biological chemistry

authors： Sandu C,Brandsch R

更新日期：2002-02-01 00:00:00

abstract：:The signal recognition particle (SRP), a ubiquitous cytoplasmic ribonucleoprotein particle, plays an essential role in promoting co-translational translocation of proteins into the endoplasmic reticulum. Here, we summarise recent progress made in the understanding of two essential SRP functions: the signal recognition...

journal_title：Biological chemistry

authors： Bui N,Strub K

更新日期：1999-02-01 00:00:00

abstract：:The heterodimer consisting of ecdysteroid receptor (EcR) and ultraspiracle (USP), both of which are members of the nuclear receptor superfamily, is considered to be the functional ecdysteroid receptor. Here we analyzed the subcellular distribution of EcR and USP fused to fluorescent proteins. The experiments were carr...

journal_title：Biological chemistry

authors： Nieva C,Gwóźdź T,Dutko-Gwóźdź J,Wiedenmann J,Spindler-Barth M,Wieczorek E,Dobrucki J,Duś D,Henrich V,Ozyhar A,Spindler KD

更新日期：2005-05-01 00:00:00

abstract：:Few therapeutic options are offered to treat inflammation and alveolar wall destruction in emphysema. The effect of recombinant human pre-elafin, an elastase inhibitor, was evaluated in porcine pancreatic elastase (PPE)-induced emphysema in C57BL/6 mice. In a first protocol, mice received a single instillation of pre-...

journal_title：Biological chemistry

authors： Janelle MF,Doucet A,Bouchard D,Bourbonnais Y,Tremblay GM

更新日期：2006-07-01 00:00:00

abstract：:Synthetic biologists aim at engineering controllable biological parts such as DNA, RNA and proteins in order to steer biological activities using external inputs. Proteins can be controlled in several ways, for instance by regulating the expression of their encoding genes with small molecules or light. However, post-t...

journal_title：Biological chemistry

authors： Di Ventura B,Mootz HD

更新日期：2019-03-26 00:00:00

abstract：:The saline extract of Bauhinia bauhinioides dry seeds was shown to inhibit cruzipain, a cysteine proteinase from Trypanosoma cruzi. The inhibitory activity was assigned to a protein with 164 amino acid residues and molecular mass of 18 034 Da that was purified by chromatography on DEAE-Sephadex, trypsin-Sepharose (rem...

journal_title：Biological chemistry

authors： de Oliveira C,Santana LA,Carmona AK,Cezari MH,Sampaio MU,Sampaio CA,Oliva ML

更新日期：2001-05-01 00:00:00

abstract：:The periplasmic ligand-binding protein ChoX is part of the ABC transport system ChoVWX that imports choline as a nutrient into the soil bacterium Sinorhizobium meliloti. We have recently reported the crystal structures of ChoX in complex with its ligands choline and acetylcholine and the structure of a fully closed bu...

journal_title：Biological chemistry

authors： Oswald C,Smits SH,Höing M,Bremer E,Schmitt L

更新日期：2009-11-01 00:00:00

abstract：:To exploit the B-lymphocyte antigen-CD20 binding capacity of the Ibritumomab tiuxetan (IBTN) monoclonal antibody (mAb) for imaging, the over-expression of B cells in non-Hodgkin's lymphoma (NHL) (a myeloproliferative disorder of the lymphatic system) was investigated. In the current investigation, we present the label...

journal_title：Biological chemistry

authors： Shah SQ,Khan AU,Khan MR

更新日期：2012-01-01 00:00:00

abstract：:In order to identify regulatory elements involved in the hepatocyte specific expression of the enzyme glutamine synthetase [GS (E.C. 6.3.1.2)] we analyzed the first intron of the rat GS gene. A sequence analysis detected clusters of potential transcription factor binding sites in regions that are hypersensitive for DN...

journal_title：Biological chemistry

authors： Gaunitz F,Gaunitz C,Papke M,Gebhardt R

更新日期：1997-01-01 00:00:00

abstract：:Abstract The R- and K-gingipain proteases of Porphyromonas gingivalis are involved in proteolysis of haemoglobin from which the defensive dimeric haem pigment is formed. Whilst oxyhaemoglobin is refractory towards K-gingipain, methaemoglobin is rapidly degraded. Ligation of methaemoglobin with N3-, which effectively b...

journal_title：Biological chemistry

authors： Smalley JW,Birss AJ,Szmigielski B,Potempa J

更新日期：2008-09-01 00:00:00

abstract：:The neurobiological mechanisms of obesity-induced peripheral neuropathy are poorly understood. We evaluated the role of Sigma-1 receptor (Sig-1R) and NMDA receptor (NMDARs) in the spinal cord in peripheral neuropathy using an animal model of high fat diet-induced diabetes. We examined the expression of Sig-1R and NMDA...

journal_title：Biological chemistry

authors： Song T,Zhao J,Ma X,Zhang Z,Jiang B,Yang Y

更新日期：2017-09-26 00:00:00

abstract：:We have previously shown that short double-stranded RNAs of specific sequence induce phosphorylation in the activation loop of splicing variant D of the transforming growth factor beta-activated protein kinase 1 (TAK1-D). Here, we further characterize this novel function of TAK1-D and the mechanisms of this dsRNA-trig...

journal_title：Biological chemistry

authors： Kodym R,Kodym E,Story MD

更新日期：2009-05-01 00:00:00

abstract：:The influence of the cellular milieu, a complex and crowded solvent, is often neglected when biomolecular structure and function are studied in vitro. To mimic the cellular environment, crowding effects are commonly induced in vitro using artificial crowding agents like Ficoll or dextran. However, it is unclear if suc...

journal_title：Biological chemistry

authors： Gnutt D,Ebbinghaus S

更新日期：2016-01-01 00:00:00

abstract：:Transforming growth factor-β (TGFβ) signaling acts as suppressor and inducer of tumor progression during the early and late stages of cancer, respectively. Some miRNAs have shown a regulatory effect on TGFβ signaling and here, we have used a combination of bioinformatics and experimental tools to show that hsa-miR-559...

journal_title：Biological chemistry

authors： Abedini Bakhshmand E,Soltani BM

更新日期：2019-04-24 00:00:00

abstract：:Glucose-dependent insulinotropic polypeptide (GIP) is an incretin hormone secreted by endocrine K-cells in response to nutrient absorption. In this study we have utilized a specific and enzymatically stable GIP receptor antagonist, (Pro3)GIP, to evaluate the contribution of endogenous GIP to insulin secretion and gluc...

journal_title：Biological chemistry

authors： Irwin N,Gault VA,Green BD,Greer B,McCluskey JT,Harriott P,O'Harte FP,Flatt PR

更新日期：2004-09-01 00:00:00

abstract：:Major vault protein (MVP) is the predominant member of a large cytosolic ribonucleoprotein particle, termed vault. We have previously shown that MVP derived from electric ray electric organ becomes phosphorylated by protein kinase C in vitro and by tyrosine kinase in vivo. Here we show that MVP from two mammalian cell...

journal_title：Biological chemistry

authors： Ehrnsperger C,Volknandt W

更新日期：2001-10-01 00:00:00

abstract：:In this review, we discuss how time-resolved Fourier transform infrared (FTIR) spectroscopy is used to understand how GTP hydrolysis is catalyzed by small GTPases and their cognate GTPase-activating proteins (GAPs). By interaction with small GTPases, GAPs regulate important signal transduction pathways and transport m...

journal_title：Biological chemistry

authors： Kötting C,Gerwert K

更新日期：2015-02-01 00:00:00