Functional analysis of the Escherichia coli molybdopterin cofactor biosynthesis protein MoeA by site-directed mutagenesis.

abstract：:A water-soluble [18O]-labeled endoperoxide derived from N,N'-di(2,3-dihydroxypropyl)-1,4-naphthalene-dipropanamide (DHPN18O2) has been shown to act as a clean chemical source of [18O]-labeled molecular singlet oxygen. This allows the assessment of the singlet oxygen (1O2) reactivity toward biological targets such as D...

journal_title：Biological chemistry

authors： Martinez GR,Medeiros MH,Ravanat JL,Cadet J,Di Mascio P

更新日期：2002-03-01 00:00:00

abstract：:Eukaryotic organisms have evolved complex and robust cellular stress response pathways to ensure maintenance of proteostasis and survival during fluctuating environmental conditions. Highly conserved stress response pathways can be triggered and coordinated at the cell-autonomous and cell-nonautonomous level by proteo...

journal_title：Biological chemistry

authors： Jones LM,Chen Y,van Oosten-Hawle P

更新日期：2020-08-27 00:00:00

abstract：:Kaposi's sarcoma-associated herpesvirus (KSHV), or human herpesvirus 8 (HHV8) is a gammaherpesvirus and the etiological agent of Kaposi's sarcoma, primary effusion lymphoma and multicentric Castleman disease. The KSHV genome contains genes for a unique group of proteins with homology to cellular interferon regulatory ...

journal_title：Biological chemistry

authors： Koch S,Schulz TF

更新日期：2017-07-26 00:00:00

abstract：:In this study, we tested the biological activity of a novel acylated form of (Pro3)glucose-dependent insulinotropic polypetide [(Pro3)GIP] prepared by conjugating palmitic acid to Lys16 to enhance its efficacy in vivo by promoting binding to albumin and extending its biological actions. Like the parent molecule (Pro3)...

journal_title：Biological chemistry

authors： Gault VA,Hunter K,Irwin N,Greer B,Green BD,Harriott P,O'Harte FP,Flatt PR

更新日期：2007-02-01 00:00:00

abstract：:Mammalian tyrosinases are key enzymes of melanin formation. Their native forms undergo complex maturation and sorting processes before being integrated into the melanosomal membrane, which greatly complicates their heterologous expression in other cell types. In the present work, we constructed several differently tru...

journal_title：Biological chemistry

authors： Cordes P,Sun W,Wolber R,Kolbe L,Klebe G,Röhm KH

更新日期：2013-05-01 00:00:00

abstract：:A putative operon of the ribosomal proteins S20 and Thx has been determined in a 1.4 kb sequenced region of T. thermophilus genomic DNA. Both genes have a promoter sequence 29 nt upstream of ORF1, possess their own Shine-Dalgarno motifs (GGAG) and are separated by only 9 nucleotides, a feature characteristic of the co...

journal_title：Biological chemistry

authors： Leontiadou F,Triantafillidou D,Choli-Papadopoulou T

更新日期：2001-07-01 00:00:00

abstract：:The purpose of this work was to study the regulative role of the glutamate receptor found earlier in the brain mitochondria. In the present work a glutamate-dependent signaling system with similar features was detected in mitochondria of the heart. The glutamate-dependent signaling system in the heart mitochondria was...

journal_title：Biological chemistry

authors： Selin AA,Lobysheva NV,Nesterov SV,Skorobogatova YA,Byvshev IM,Pavlik LL,Mikheeva IB,Moshkov DA,Yaguzhinsky LS,Nartsissov YR

更新日期：2016-05-01 00:00:00

abstract：:Only one transcript for KLK10 was identified by RT-PCR in lung tissue, whereas KLK11 expressed at least four alternative transcripts. Quantitative analysis of KLK10 and KLK11 expression levels was assessed by real-time PCR, in a cohort of 47 patients with non-small-cell lung cancer (NSCLC). Expression levels of these ...

journal_title：Biological chemistry

authors： Planque C,Aïnciburu M,Heuzé-Vourc'h N,Régina S,de Monte M,Courty Y

更新日期：2006-06-01 00:00:00

abstract：:Mammalian apolipoproteinB (apoB) RNA editing is a site-specific deamination reaction that mediates the C to U conversion responsible for apoB48 production in the mammalian small intestine. This process is not detected in chicken apoB RNA. Mammalian apoB RNA editing is mediated by a multicomponent enzyme complex that i...

journal_title：Biological chemistry

authors： Anant S,Yu H,Davidson NO

更新日期：1998-08-01 00:00:00

abstract：:The expression of the cysteine protease cathepsin B is increased in early stages of human breast cancer.To assess the potential role of cathepsin B in premalignant progression of breast epithelial cells, we employed a 3D reconstituted basement membrane overlay culture model of MCF10A human breast epithelial cells and ...

journal_title：Biological chemistry

authors： Mullins SR,Sameni M,Blum G,Bogyo M,Sloane BF,Moin K

更新日期：2012-12-01 00:00:00

abstract：:During the last years, the use of therapeutic glycoproteins has increased strikingly. Glycosylation of recombinant glycoproteins is of major importance in biotechnology, as the glycan composition of recombinant glycoproteins impacts their pharmacological properties. The terminal position of N-linked complex glycans in...

journal_title：Biological chemistry

authors： Werner A,Horstkorte R,Glanz D,Biskup K,Blanchard V,Berger M,Bork K

更新日期：2012-08-01 00:00:00

abstract：:The cloning of a Ca2+(0)-sensing receptor (CaR) from parathyroid and kidney, coupled with the identification of inherited disorders in Ca2+(0)-sensing resulting from inactivating or activating mutations of this CaR, has provided substantial insights into how Ca2+(0) is regulated at a systemic level. The CaR plays a ce...

journal_title：Biological chemistry

authors： Chattopadhyay N,Vassilev PM,Brown EM

更新日期：1997-08-01 00:00:00

abstract：:Type I interferons (IFNs) produced primarily by plasmacytoid dendritic cells (pDCs) as part of the innate immune response to infectious agents induce the maturation of myeloid DCs and enhance antigen presentation. Type I IFNs also enhance apoptosis of virus-infected cells, stimulate cross priming and enhanced presenta...

journal_title：Biological chemistry

authors： Tovey MG,Lallemand C,Thyphronitis G

更新日期：2008-05-01 00:00:00

abstract：:Transcriptional enhancers are short (200-1500 base pairs) DNA segments that are able to dramatically boost transcription from the promoter of a target gene. Originally discovered in simian virus 40 (SV40), a small DNA virus, transcription enhancers were soon also found in immunoglobulin genes and other cellular genes ...

journal_title：Biological chemistry

authors： Schaffner W

更新日期：2015-04-01 00:00:00

abstract：:The ubiquitous calpains, mu- and m-calpain, have been implicated in essential physiological processes and various pathologies. Cell-permeable specific inhibitors are important tools to elucidate the roles of calpains in cultivated cells and animal models. The synthetic N-acetylated 27-mer peptide derived from exon B o...

journal_title：Biological chemistry

authors： Gil-Parrado S,Assfalg-Machleidt I,Fiorino F,Deluca D,Pfeiler D,Schaschke N,Moroder L,Machleidt W

更新日期：2003-03-01 00:00:00

abstract：:The molecular architectures of the catalytic core of protein phosphatase 1 (PP1) and protein phosphatase 2B (PP2B) are similar, and both contain a beta12-beta13 loop that consists of non-conserved residues. A truncation mutant containing the PP2B catalytic domain has previously been constructed in our laboratory, and ...

journal_title：Biological chemistry

authors： Xie XJ,Xue CZ,Huang W,Yu DY,Wei Q

更新日期：2006-10-01 00:00:00

abstract：:DEAD-box helicases catalyze RNA duplex unwinding in an ATP-dependent reaction. Members of the DEAD-box helicase family consist of a common helicase core formed by two RecA-like domains. According to the current mechanistic model for DEAD-box mediated RNA unwinding, binding of RNA and ATP triggers a conformational chan...

journal_title：Biological chemistry

authors： Rudolph MG,Klostermeier D

更新日期：2015-08-01 00:00:00

abstract：:A number of unexpected pathophysiological connections linking different neurodegenerative diseases have emerged over the past decade. An example is provided by prion and Alzheimer's diseases. Despite being distinct pathologies, these disorders share several neurotoxic mechanisms, including accumulation of misfolded pr...

journal_title：Biological chemistry

authors： Elezgarai SR,Biasini E

更新日期：2016-11-01 00:00:00

abstract：:Based on its monophenolic structure and given its pharmacological and toxicological importance, the ability of tyrosinase to oxidize acetaminophen was studied for the first time. Progress curves showed a transient phase characteristic of the monophenolase activity of tyrosinase prior to attaining the steady-state. The...

journal_title：Biological chemistry

authors： Valero E,Varón R,García-Carmona F

更新日期：2002-12-01 00:00:00

abstract：:Few therapeutic options are offered to treat inflammation and alveolar wall destruction in emphysema. The effect of recombinant human pre-elafin, an elastase inhibitor, was evaluated in porcine pancreatic elastase (PPE)-induced emphysema in C57BL/6 mice. In a first protocol, mice received a single instillation of pre-...

journal_title：Biological chemistry

authors： Janelle MF,Doucet A,Bouchard D,Bourbonnais Y,Tremblay GM

更新日期：2006-07-01 00:00:00

abstract：:Cancer cells are relatively resistant to endoplasmic reticulum (ER) stress-induced apoptosis. However, the underlying mechanisms remain largely unclear. We observed that the microRNAs miR-221/222 are associated with apoptosis regulation under ER stress in human hepatocellular carcinoma (HCC) cells. Induction of ER str...

journal_title：Biological chemistry

authors： Dai R,Li J,Liu Y,Yan D,Chen S,Duan C,Liu X,He T,Li H

更新日期：2010-07-01 00:00:00

abstract：:Bone morphogenetic proteins (BMP) are dimeric factors initiating several distinct signaling cascades by binding to two types of transmembrane serine/threonine kinase receptors (BRI and BRII), and are thus regulating several steps in embryonal development and adult tissue homeostasis. BMP-2 contains two symmetrical pai...

journal_title：Biological chemistry

authors： Knaus P,Sebald W

更新日期：2001-08-01 00:00:00

abstract：:Neuritic plaques of Alzheimer patients are composed of multiple protein components. Among them, the amyloid beta-peptides (Abeta) 1-40/42 and further N- and C-terminally modified fragments of Abeta are highly abundant. Most prominent are the isoaspartate (isoAsp)-Abeta peptides and pyroglutamyl (pGlu)-Abeta. While pGl...

journal_title：Biological chemistry

authors： Böhme L,Hoffmann T,Manhart S,Wolf R,Demuth HU

更新日期：2008-08-01 00:00:00

abstract：:Silver nanoparticles (AgNPs) have many biological applications in biomedicine, biotechnology and other life sciences. Depending on the size, shape and the type of carrier, AgNPs demonstrate different physical and chemical properties. AgNPs have strong antimicrobial, antiviral and antifungal activity, thus they are use...

journal_title：Biological chemistry

authors： Szmyd R,Goralczyk AG,Skalniak L,Cierniak A,Lipert B,Filon FL,Crosera M,Borowczyk J,Laczna E,Drukala J,Klein A,Jura J

更新日期：2013-01-01 00:00:00

abstract：:Most excitatory synaptic transmissions in the central nervous system are mediated by the neurotransmitter glutamate. Binding of glutamate released from the presynaptic membrane causes glutamate receptors in the postsynaptic membrane to open, which results in a transient depolarization of the postsynaptic membrane. The...

journal_title：Biological chemistry

authors： Nakagawa T,Cheng Y,Sheng M,Walz T

更新日期：2006-02-01 00:00:00

abstract：:Lipids serve many distinct functions in cellular homeostasis such as membrane organization, as a platform for membrane function and protein/protein or protein/lipid interaction, energy storage, as well as secondary messengers in signal transduction. Perturbations in lipid homeostasis may result in abnormal cellular fu...

journal_title：Biological chemistry

authors： Xiang Y,Lam SM,Shui G

更新日期：2015-12-01 00:00:00

abstract：:Two new fluorescent labels are presented that are optimized for excitation with He/Ne laser and red diode lasers. Application in FCS and labeling of proteins and oligomers are demonstrated. A strong rise of quantum yield and emission life time upon binding to biomolecules are characteristic features of the dyes. ...

journal_title：Biological chemistry

authors： Czerney P,Lehmann F,Wenzel M,Buschmann V,Dietrich A,Mohr GJ

更新日期：2001-03-01 00:00:00

abstract：:M.EcoRV is an alpha-adenine DNA methyltransferase. According to structure predictions, the enzyme consists of a catalytic domain, which has a structure similar to all other DNA-methyltransferases, and a smaller DNA-recognition domain. We have investigated this enzyme by random mutagenesis, using error-prone PCR, follo...

journal_title：Biological chemistry

authors： Friedrich T,Roth M,Helm-Kruse S,Jeltsch A

更新日期：1998-04-01 00:00:00

abstract：:The solute carrier family SLC10 consists of seven members, including the bile acid transporters Na+/taurocholate co-transporting polypeptide (NTCP) and apical sodium-dependent bile acid transporter (ASBT), the steroid sulfate transporter SOAT as well as four orphan carriers (SLC10A3, SLC10A4, SLC10A5 and SLC10A7). Pre...

journal_title：Biological chemistry

authors： Noppes S,Müller SF,Bennien J,Holtemeyer M,Palatini M,Leidolf R,Alber J,Geyer J

更新日期：2019-09-25 00:00:00

abstract：:The surface of spherical, nonporous silica nanoparticles (SiO2-NPs) was modified with gadolinium (Gd) complexes, fluorophores, and cell-penetrating peptides to achieve multifunctionality on a single particle. The Gd surface concentrations were 9-16 μmol/g resulting in nanomaterials with high local longitudinal and tra...

journal_title：Biological chemistry

authors： Joshi R,Feldmann V,Koestner W,Detje C,Gottschalk S,Mayer HA,Sauer MG,Engelmann J

更新日期：2013-01-01 00:00:00