Abstract:
:Synthetic biologists aim at engineering controllable biological parts such as DNA, RNA and proteins in order to steer biological activities using external inputs. Proteins can be controlled in several ways, for instance by regulating the expression of their encoding genes with small molecules or light. However, post-translationally modifying pre-existing proteins to regulate their function or localization leads to faster responses. Conditional splicing of internal protein domains, termed inteins, is an attractive methodology for this purpose. Here we discuss methods to control intein activity with a focus on those compatible with applications in living cells.
journal_name
Biol Chemjournal_title
Biological chemistryauthors
Di Ventura B,Mootz HDdoi
10.1515/hsz-2018-0309subject
Has Abstractpub_date
2019-03-26 00:00:00pages
467-475issue
4eissn
1431-6730issn
1437-4315pii
/j/bchm.ahead-of-print/hsz-2018-0309/hsz-2018-0309journal_volume
400pub_type
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