FAM134B promotes adipogenesis by increasing vesicular activity in porcine and 3T3-L1 adipocytes.


:Family with sequence similarity 134, Member B (FAM134B), is a cis-Golgi transmembrane protein that is known to be necessary for the long-term survival of nociceptive and autonomic ganglion neurons. Recent work has shown that FAM134B plays a pivotal role in autophagy-mediated turnover of endoplasmic reticulum (ER) membranes, tumor inhibition and lipid homeostasis. In this study, we provide mechanistic links between FAM134B and ARF-related protein 1 (ARFRP1) and further show that FAM134B resides in the Golgi apparatus. Here, we found that FAM134B increased lipid accumulation in adipocytes. Transport vehicle number and ADP-ribosylation factor (ARF) family gene expression were also increased by FAM134B overexpression, suggesting that vesicular transport activity enhanced lipid accumulation. ARF-related protein 1 (ARFRP1) is a GTPase that promotes protein trafficking. We show that FAM134B regulates the expression of ARFRP1, and the knockdown of ARFRP1 abolishes enhancement on lipid accumulation caused by FAM134B. In addition, FAM134B upregulates the PAT family protein (PAT), which associates with the lipid droplets (LDs) surface and promotes lipolysis by recruiting adipocyte triglyceride lipase (ATGL). These findings indicate that FAM134B promotes lipid accumulation and adipogenic differentiation by increasing vesicle transport activity in the Golgi apparatus and inhibiting the lipolysis of LDs.


Biol Chem


Biological chemistry


Cai M,Chen J,Yu C,Xi L,Jiang Q,Wang Y,Wang X




Has Abstract


2019-03-26 00:00:00














  • Annexins in plasma membrane repair.

    abstract::Disruption of the plasma membrane poses deadly threat to eukaryotic cells and survival requires a rapid membrane repair system. Recent evidence reveal various plasma membrane repair mechanisms, which are required for cells to cope with membrane lesions including membrane fusion and replacement strategies, remodeling o...

    journal_title:Biological chemistry

    pub_type: 杂志文章,评审


    authors: Boye TL,Nylandsted J

    更新日期:2016-10-01 00:00:00

  • The human steroid hydroxylases CYP1B1 and CYP11B2.

    abstract::Major advances have been made during the last decade in our understanding of adrenal steroid hormone biosynthesis. Two key players in these pathways are the human mitochondrial cytochrome P450 enzymes CYP11B1 and CYP11B2, which catalyze the final steps in the biosynthesis of cortisol and aldosterone. Using data from m...

    journal_title:Biological chemistry

    pub_type: 杂志文章,评审


    authors: Bureik M,Lisurek M,Bernhardt R

    更新日期:2002-10-01 00:00:00

  • Electron-nuclear double resonance spectroscopy investigation of 4-hydroxybutyryl-CoA dehydratase from Clostridium aminobutyricum: comparison with other flavin radical enzymes.

    abstract::A FAD and [4Fe-4S]cluster-containing enzyme from Clostridium aminobutyricum catalyses the reversible dehydration of 4-hydroxybutyryl-CoA to crotonyl-CoA which involves the cleavage of an unactivated C-H bond at the beta-carbon. Transient oxidation of the substrate to an enoxy radical by FAD might facilitate the remova...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Cinkaya I,Buckel W,Medina M,Gomez-Moreno C,Cammack R

    更新日期:1997-08-01 00:00:00

  • The polysaccharide scaffold of PrP 27-30 is a common compound of natural prions and consists of alpha-linked polyglucose.

    abstract::An inert polysaccharide scaffold identified as a 5-15% component of prion rods (PrP 27-30) is unambiguously distinguishable from the N-glycosyl groups and the GPI anchor of PrP, and consists predominantly of 1,4-linked glucose with some branching via 1,4,6-linked glucose. We show that this polysaccharide scaffold is a...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Dumpitak C,Beekes M,Weinmann N,Metzger S,Winklhofer KF,Tatzelt J,Riesner D

    更新日期:2005-11-01 00:00:00

  • Activation of progelatinase A by mammalian legumain, a recently discovered cysteine proteinase.

    abstract::The activation of progelatinase A to gelatinase A requires cleavage of an asparaginyl bond to form the N-terminus of the mature enzyme. We have asked whether the activation can be mediated by legumain, the recently discovered lysosomal cysteine proteinase that is specific for hydrolysis of asparaginyl bonds. Addition ...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Chen JM,Fortunato M,Stevens RA,Barrett AJ

    更新日期:2001-05-01 00:00:00

  • Transcriptional activity of GLI1 is negatively regulated by protein kinase A.

    abstract::The transcription factor GLI1 is one of three vertebrate members of the GLI family, which is characterized by a highly conserved DNA-binding domain of five zinc fingers. We have analyzed whether human GLI1 is a target of PKA regulation. It was found that PKA inhibits GLI1 transcriptional activity. However, no evidence...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Kaesler S,Lüscher B,Rüther U

    更新日期:2000-07-01 00:00:00

  • 'Dipeptidyl peptidase-IV activity and/or structure homologs' (DASH) in growth-modulated glioma cell lines.

    abstract::Dipeptidyl peptidase-IV has been demonstrated to play a role in cancer biology by many authors. Since then, additional proteins possessing similar enzymatic activity have been described and their role in cancerogenesis has been hypothesized. To assess the complexity of these 'Dipeptidyl peptidase-IV activity and/or st...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Sedo A,Busek P,Scholzová E,Malík R,Vlasicová K,Janácková S,Mares V

    更新日期:2004-06-01 00:00:00

  • The FIRE3-mediated sterol response of the FAS promoter requires NF-Y/CBF as a coactivator.

    abstract::The transcription of the fatty acid synthase (FAS) gene is regulated by the sterol status of the cell via cleavage of the sterol regulatory element-binding protein (SREBP). When human HepG2 hepatoma cells were cotransfected with an expression plasmid for mature SREBP-1a together with FAS promoter/reporter constructs s...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Wolf SS,Roder K,Sickinger S,Schweizer M

    更新日期:2001-07-01 00:00:00

  • Tapasin and other chaperones: models of the MHC class I loading complex.

    abstract::MHC (major histocompatibility complex) class I molecules bind intracellular virus-derived peptides in the endoplasmic reticulum (ER) and present them at the cell surface to cytotoxic T lymphocytes. Peptide-free class I molecules at the cell surface, however, could lead to aberrant T cell killing. Therefore, cells ensu...

    journal_title:Biological chemistry

    pub_type: 杂志文章,评审


    authors: Wright CA,Kozik P,Zacharias M,Springer S

    更新日期:2004-09-01 00:00:00

  • Multifunctional biomaterial coatings: synthetic challenges and biological activity.

    abstract::A controlled interaction of materials with their surrounding biological environment is of great interest in many fields. Multifunctional coatings aim to provide simultaneous modulation of several biological signals. They can consist of various combinations of bioactive, and bioinert components as well as of reporter m...

    journal_title:Biological chemistry

    pub_type: 杂志文章,评审


    authors: Pagel M,Beck-Sickinger AG

    更新日期:2017-01-01 00:00:00

  • Purification of chrysancorin, a novel antifungal protein with mitogenic activity from garland chrysanthemum seeds.

    abstract::A novel antifungal protein, designated chrysancorin, was isolated from seeds of Chrysanthemum coronarium var. spatiosum with a procedure involving ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue resin, ion exchange chromatography on SP-Sepharose and FPLC-gel filtration on Superd...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Wang H,Ye XY,Ng TB

    更新日期:2001-06-01 00:00:00

  • Structural basis of [NiFe] hydrogenase maturation by Hyp proteins.

    abstract::[NiFe] hydrogenases catalyze reversible hydrogen production/consumption. The active site of [NiFe] hydrogenases contains a complex NiFe(CN)2CO center, and the biosynthesis/maturation of these enzymes is a complex and dynamic process, primarily involving six Hyp proteins (HypABCDEF). HypA and HypB are involved in the N...

    journal_title:Biological chemistry

    pub_type: 杂志文章,评审


    authors: Watanabe S,Sasaki D,Tominaga T,Miki K

    更新日期:2012-09-08 00:00:00

  • Short double-stranded RNAs of specific sequence activate ribosomal TAK1-D and induce a global inhibition of translation.

    abstract::We have previously shown that short double-stranded RNAs of specific sequence induce phosphorylation in the activation loop of splicing variant D of the transforming growth factor beta-activated protein kinase 1 (TAK1-D). Here, we further characterize this novel function of TAK1-D and the mechanisms of this dsRNA-trig...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Kodym R,Kodym E,Story MD

    更新日期:2009-05-01 00:00:00

  • Imaging morphological details and pathological differences of red blood cells using tapping-mode AFM.

    abstract::The surface topography of red blood cells (RBCs) was investigated under near-physiological conditions using atomic force microscopy (AFM). An immobilization protocol was established where RBCs are coupled via molecular bonds of the membrane glycoproteins to wheat germ agglutinin (WGA), which is covalently and flexibly...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Kamruzzahan AS,Kienberger F,Stroh CM,Berg J,Huss R,Ebner A,Zhu R,Rankl C,Gruber HJ,Hinterdorfer P

    更新日期:2004-10-01 00:00:00

  • The pathobiochemistry of nitrogen dioxide.

    abstract::Nitrogen dioxide (*NO2) is an oxidizing free radical which can initiate a variety of destructive pathways in living systems, and several diseases are suspected to be connected with both exogenously and endogenously formed *NO2. Peroxynitrite (ONOO-/ONOOH) is believed to be an important endogenous source of *NO2 radica...

    journal_title:Biological chemistry

    pub_type: 杂志文章,评审


    authors: Kirsch M,Korth HG,Sustmann R,de Groot H

    更新日期:2002-03-01 00:00:00

  • Attenuated kinin release from human neutrophil elastase-pretreated kininogens by tissue and plasma kallikreins.

    abstract::Components of kinin-forming systems operating at inflammatory sites are likely to interact with elastase that is released by recruited neutrophils and may, at least temporarily, constitute the major proteolytic activity present at these sites. The aim of this work was to determine the effect of kininogen degradation b...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Dulinski R,Suder P,Guevara-Lora I,Rapała-Kozik M,Potempa J,Silberring J,Imamura T,Travis J,Kozik A

    更新日期:2003-06-01 00:00:00

  • Analysis of the autoproteolytic activity of the recombinant helper component proteinase from zucchini yellow mosaic virus.

    abstract::The multifunctional helper component proteinase (HC-Pro) of potyviruses contains an autoproteolytic function that, together with the protein 1 (P1) and NIa proteinase, processes the polyprotein into mature proteins. In this study, we analysed the autoproteolytic active domain of zucchini yellow mosaic virus (ZYMV) HC-...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Boonrod K,Füllgrabe MW,Krczal G,Wassenegger M

    更新日期:2011-10-01 00:00:00

  • Activation profiles of human kallikrein-related peptidases by matrix metalloproteinases.

    abstract::The 15 human kallikrein-related peptidases (KLKs) are clinically important biomarkers and therapeutic targets of interest in inflammation, cancer, and neurodegenerative disease. KLKs are secreted as inactive pro-forms (pro-KLKs) that are activated extracellularly by specific proteolytic release of their amino-terminal...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Yoon H,Blaber SI,Li W,Scarisbrick IA,Blaber M

    更新日期:2013-01-01 00:00:00

  • Hsp70 chaperone systems: diversity of cellular functions and mechanism of action.

    abstract::Hsp70 chaperone systems play an essential role in the life cycle of many proteins not only in an hostile environment but also under normal growth conditions. In the course of evolution the diversification of functions was accompanied by an amplification of components of the Hsp70 system. Here strategies are reviewed h...

    journal_title:Biological chemistry

    pub_type: 杂志文章,评审


    authors: Mayer MP,Bukau B

    更新日期:1998-03-01 00:00:00

  • Kinetics of autocatalytic zymogen activation measured by a coupled reaction: pepsinogen autoactivation.

    abstract::A kinetic study was performed of a model for an autocatalytic zymogen activation process involving both intra- and intermolecular routes, to which a chromogenic reaction in which the active enzyme acts upon one of its substrates was coupled to continuously monitor the reaction. Kinetic equations describing the evoluti...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Fuentes ME,Varón R,García-Moreno M,Valero E

    更新日期:2005-07-01 00:00:00

  • Nervous system pathology: the fibrin perspective.

    abstract::Studies of extracellular matrix (ECM) biology in the nervous system have mainly focused on laminin, fibronectin and tenascin-R, proteins that are present during nervous system development and normal function. However, during disease, fibrin, which physiologically is not present in the nervous tissue, is detected at ne...

    journal_title:Biological chemistry

    pub_type: 杂志文章,评审


    authors: Akassoglou K,Strickland S

    更新日期:2002-01-01 00:00:00

  • Isoaspartate-containing amyloid precursor protein-derived peptides alter efficacy and specificity of potential beta-secretases.

    abstract::Neuritic plaques of Alzheimer patients are composed of multiple protein components. Among them, the amyloid beta-peptides (Abeta) 1-40/42 and further N- and C-terminally modified fragments of Abeta are highly abundant. Most prominent are the isoaspartate (isoAsp)-Abeta peptides and pyroglutamyl (pGlu)-Abeta. While pGl...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Böhme L,Hoffmann T,Manhart S,Wolf R,Demuth HU

    更新日期:2008-08-01 00:00:00

  • Reverse catalysis of elastase from porcine pancreas in frozen aqueous systems.

    abstract::The reverse action of a trypsin-free elastase isolated from porcine pancreas was studied in frozen aqueous systems. Under frozen state conditions, porcine pancreatic elastase was able to catalyse peptide bond formation more effectively than in solution at room temperature. The acceptance of free amino acids as nucleop...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Haensler M,Wehofsky N,Gerisch S,Wissmann JD,Jakubke HD

    更新日期:1998-01-01 00:00:00

  • Self-organization versus Watchmaker: stochastic dynamics of cellular organization.

    abstract::The cell, as a molecular system, is often interpreted in terms of complex clockworks, and the design charts of mechanical and electrical engineering are assumed to provide adequate approximations for the description of cellular organization. However, a growing body of experimental evidence obtained through the observa...

    journal_title:Biological chemistry

    pub_type: 杂志文章,评审


    authors: Kurakin A

    更新日期:2005-03-01 00:00:00

  • Trypsin: a case study in the structural determinants of enzyme specificity.

    abstract::Trypsin and chymotrypsin have similar tertiary structures, although very different substrate specificities. Trypsin hydrolyzes peptides at Lys/Arg residues while chymotrypsin recognizes large hydrophobic residues. Recent work has shown that trypsin is not converted into a protease with chymotrypsin-like activity when ...

    journal_title:Biological chemistry

    pub_type: 杂志文章,评审


    authors: Hedstrom L

    更新日期:1996-07-01 00:00:00

  • Agonist-stimulated pathways of calcium signaling in pancreatic acinar cells.

    abstract::In pancreatic acinar cells stimulation of different intracellular pathways leads to different patterns of Ca2+ signaling. Bombesin induces activation of both phosphatidylinositol 4,5-bisphosphate (PIP2)-specific phospholipase C (PLC) and phospholipase D (PLD). The latter leads to generation of diacylglycerol (DAG) in ...

    journal_title:Biological chemistry

    pub_type: 杂志文章,评审


    authors: Schulz I,Krause E,González A,Göbel A,Sternfeld L,Schmid A

    更新日期:1999-07-01 00:00:00

  • Tailor-made dyes for fluorescence correlation spectroscopy (FCS).

    abstract::Two new fluorescent labels are presented that are optimized for excitation with He/Ne laser and red diode lasers. Application in FCS and labeling of proteins and oligomers are demonstrated. A strong rise of quantum yield and emission life time upon binding to biomolecules are characteristic features of the dyes. ...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Czerney P,Lehmann F,Wenzel M,Buschmann V,Dietrich A,Mohr GJ

    更新日期:2001-03-01 00:00:00

  • Characterization of the nuclear import mechanisms of HSV-1 UL31.

    abstract::As an important protein, UL31 has been demonstrated to play multiple roles in herpes simplex virus 1 (HSV-1) replication. Previous studies showed that UL31 predominantly locates in the nucleus in chemical fixed cells and live cells, however, the determining mechanisms for its nuclear translocation is not clear. In the...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Cai M,Si J,Li X,Zeng Z,Li M

    更新日期:2016-06-01 00:00:00

  • Characterization of the subcellular localization and nuclear import molecular mechanisms of herpes simplex virus 1 UL2.

    abstract::As a crucial protein, the herpes simplex virus 1 (HSV-1) UL2 protein has been shown to take part in various stages of viral infection, nonetheless, its exact subcellular localization and transport molecular determinants are not well known thus far. In the present study, by using live cells fluorescent microscopy assay...

    journal_title:Biological chemistry

    pub_type: 杂志文章


    authors: Cai M,Huang Z,Liao Z,Chen T,Wang P,Jiang S,Chen D,Peng T,Bian Y,Hong G,Yang H,Zeng Z,Li X,Li M

    更新日期:2017-04-01 00:00:00

  • Kallikrein-related peptidase (KLK) family mRNA variants and protein isoforms in hormone-related cancers: do they have a function?

    abstract::The kallikrein-related peptidase (KLK) gene family of 15 serine proteases encodes many proteins, including prostate specific antigen (PSA or KLK3), that are well described and/or are potential biomarkers for hormone-related cancers. Variant mRNA transcripts produced by alternative splicing, polyadenylation or AUG site...

    journal_title:Biological chemistry

    pub_type: 杂志文章,评审


    authors: Tan OL,Whitbread AK,Clements JA,Dong Y

    更新日期:2006-06-01 00:00:00