Correlating structure and ligand affinity in drug discovery: a cautionary tale involving second shell residues.

abstract：:In this review, we discuss how time-resolved Fourier transform infrared (FTIR) spectroscopy is used to understand how GTP hydrolysis is catalyzed by small GTPases and their cognate GTPase-activating proteins (GAPs). By interaction with small GTPases, GAPs regulate important signal transduction pathways and transport m...

journal_title：Biological chemistry

authors： Kötting C,Gerwert K

更新日期：2015-02-01 00:00:00

abstract：:Cellular volume regulation is fundamental for numerous physiological processes. The volume-regulated anion channel, VRAC, plays a crucial role in regulatory volume decrease. This channel, which is ubiquitously expressed in vertebrates, has been vastly characterized by electrophysiological means. It opens upon cell swe...

journal_title：Biological chemistry

authors： Stauber T

更新日期：2015-09-01 00:00:00

abstract：:Synthetic biologists aim at engineering controllable biological parts such as DNA, RNA and proteins in order to steer biological activities using external inputs. Proteins can be controlled in several ways, for instance by regulating the expression of their encoding genes with small molecules or light. However, post-t...

journal_title：Biological chemistry

authors： Di Ventura B,Mootz HD

更新日期：2019-03-26 00:00:00

abstract：:A putative operon of the ribosomal proteins S20 and Thx has been determined in a 1.4 kb sequenced region of T. thermophilus genomic DNA. Both genes have a promoter sequence 29 nt upstream of ORF1, possess their own Shine-Dalgarno motifs (GGAG) and are separated by only 9 nucleotides, a feature characteristic of the co...

journal_title：Biological chemistry

authors： Leontiadou F,Triantafillidou D,Choli-Papadopoulou T

更新日期：2001-07-01 00:00:00

abstract：:Abstract The R- and K-gingipain proteases of Porphyromonas gingivalis are involved in proteolysis of haemoglobin from which the defensive dimeric haem pigment is formed. Whilst oxyhaemoglobin is refractory towards K-gingipain, methaemoglobin is rapidly degraded. Ligation of methaemoglobin with N3-, which effectively b...

journal_title：Biological chemistry

authors： Smalley JW,Birss AJ,Szmigielski B,Potempa J

更新日期：2008-09-01 00:00:00

abstract：:Understanding the molecular mechanisms behind extreme temperature stability is of relevance for the protein folding problem and for designing proteins for industrial and medical applications. A powerful approach for understanding the structural basis of thermostability is the comparison of high resolution structures o...

journal_title：Biological chemistry

authors： Macedo-Ribeiro S,Darimont B,Sterner R

更新日期：1997-03-01 00:00:00

abstract：:The influence of the hyaluronan-binding protease (PHBSP), a plasma enzyme with FVII- and pro-urokinase-activating potency, on components of the contact phase (kallikrein/kinin) system was investigated. No activation or cleavage of the proenzymes involved in the contact phase system was observed. The pro-cofactor high ...

journal_title：Biological chemistry

authors： Etscheid M,Beer N,Fink E,Seitz R,Johannes D

更新日期：2002-10-01 00:00:00

abstract：:Zinc (Zn2+) is the most abundant trace element in cells and is essential for a vast number of catalytic, structural, and regulatory processes. Mounting evidence indicates that like calcium (Ca2+), intracellular Zn2+ pools are redistributed for specific cellular functions. This occurs through the regulation of 24 Zn2+ ...

journal_title：Biological chemistry

authors： Hennigar SR,Kelleher SL

更新日期：2012-07-01 00:00:00

abstract：:Abstract Few genes are known to be involved in renal cell carcinoma (RCC) development and progression. The cell-specific transcription factor hepatocyte nuclear factor 4 alpha (HNF4 alpha) is down-regulated in RCC and we have shown that HNF4 alpha inhibits cell proliferation in the embryonic kidney cell line HEK293. T...

journal_title：Biological chemistry

authors： Grigo K,Wirsing A,Lucas B,Klein-Hitpass L,Ryffel GU

更新日期：2008-02-01 00:00:00

abstract：:The ligand-activated aryl hydrocarbon receptor (AHR) is known to modulate many genes in a highly cell-specific manner, either directly or indirectly via secondary effects. In contrast, little is known about the effects of AHR deficiency on gene expression balance. We compared the transcriptome of CD4 T cells from AHR-...

journal_title：Biological chemistry

authors： Frericks M,Temchura VV,Majora M,Stutte S,Esser C

更新日期：2006-09-01 00:00:00

abstract：:Fluorescence correlation spectroscopy (FCS) was used in monitoring human parvovirus B19 virus-like particle (VLP) antibody complexes from acute phase and past-immunity serum samples. The Oregon Green 488-labeled VLPs gave an average diffusion coefficient of 1.7 x 10(-7) cm2 s(-1) with an apparent hydrodynamic radius o...

journal_title：Biological chemistry

authors： Toivola J,Michel PO,Gilbert L,Lahtinen T,Marjomäki V,Hedman K,Vuento M,Oker-Blom C

更新日期：2004-01-01 00:00:00

abstract：:Based on studies presented here and other published experiments performed with surviving tissue preparations, with transfected cells and with cells that constitutively express the human angiotensin I converting enzyme ACE and B2 receptors, we concluded the following: ACE inhibitors and other endogenous peptides that r...

journal_title：Biological chemistry

authors： Erdös EG,Marcic BM

更新日期：2001-01-01 00:00:00

abstract：:During the last years, the use of therapeutic glycoproteins has increased strikingly. Glycosylation of recombinant glycoproteins is of major importance in biotechnology, as the glycan composition of recombinant glycoproteins impacts their pharmacological properties. The terminal position of N-linked complex glycans in...

journal_title：Biological chemistry

authors： Werner A,Horstkorte R,Glanz D,Biskup K,Blanchard V,Berger M,Bork K

更新日期：2012-08-01 00:00:00

abstract：:In order to identify regulatory elements involved in the hepatocyte specific expression of the enzyme glutamine synthetase [GS (E.C. 6.3.1.2)] we analyzed the first intron of the rat GS gene. A sequence analysis detected clusters of potential transcription factor binding sites in regions that are hypersensitive for DN...

journal_title：Biological chemistry

authors： Gaunitz F,Gaunitz C,Papke M,Gebhardt R

更新日期：1997-01-01 00:00:00

abstract：:The BCL2 family of genes (B-cell CLL/lymphoma 2; Bcl-2) plays a pivotal role in the highly regulated process of apoptosis. We have recently cloned a newly identified member of this family, BCL2L12, which was found to be differentially expressed in many tumors. It is known that topotecan and methotrexate act through in...

journal_title：Biological chemistry

authors： Floros KV,Talieri M,Scorilas A

更新日期：2006-12-01 00:00:00

abstract：:Human septins comprise a family of 13 genes that encode for >30 protein isoforms with ubiquitous and tissue-specific expressions. Septins are GTP-binding proteins that assemble into higher-order oligomers and filamentous polymers, which associate with cell membranes and the cytoskeleton. In the last decade, much progr...

journal_title：Biological chemistry

authors： Dolat L,Hu Q,Spiliotis ET

更新日期：2014-02-01 00:00:00

abstract：:Bradykinin (BK) is a vasorelaxant, algesic and inflammatory agent. Angiotensin II (AngII) is known to control vascular tone and promote growth, inflammation and artherogenesis. There is evidence for cross talking between BK and AngII receptors. Therefore, the effect of lack of kinin receptors was assessed in mice with...

journal_title：Biological chemistry

authors： Rodrigues ES,Martin RP,Felipe SA,Bader M,Oliveira SM,Shimuta SI

更新日期：2009-09-01 00:00:00

abstract：:Drug-induced phospholipidosis is a lysosomal storage disorder characterized by excessive accumulation of phospholipids. Its cellular mechanism is still not well understood, but it is known that cationic amphiphilic drugs can induce it. These drugs have a hydrophilic amine head group that can be protonated in the endol...

journal_title：Biological chemistry

authors： Breiden B,Sandhoff K

更新日期：2019-12-18 00:00:00

abstract：:There are enzymatic and non-enzymatic mechanisms that generate NO* from nitrite in blood, stomach, saliva, urine and skin. In blood vessels, nitrite-derived NO* can provide protection via compensatory vasodilation during hypoxia, and in various body fluids it may have antibacterial activity. In the skin, nitrite-deriv...

journal_title：Biological chemistry

authors： Suschek CV,Schewe T,Sies H,Kröncke KD

更新日期：2006-05-01 00:00:00

abstract：:Tissue kallikrein KLK1 and the kallikrein-related peptidases KLK2-15 are a subfamily of serine proteases that have defined or proposed roles in a range of central nervous system (CNS) and non-CNS pathologies. To further understand their potential activity in multiple sclerosis (MS), serum levels of KLK1, 6, 7, 8 and 1...

journal_title：Biological chemistry

authors： Scarisbrick IA,Linbo R,Vandell AG,Keegan M,Blaber SI,Blaber M,Sneve D,Lucchinetti CF,Rodriguez M,Diamandis EP

更新日期：2008-06-01 00:00:00

abstract：:We have previously shown that short double-stranded RNAs of specific sequence induce phosphorylation in the activation loop of splicing variant D of the transforming growth factor beta-activated protein kinase 1 (TAK1-D). Here, we further characterize this novel function of TAK1-D and the mechanisms of this dsRNA-trig...

journal_title：Biological chemistry

authors： Kodym R,Kodym E,Story MD

更新日期：2009-05-01 00:00:00

abstract：:The conversion of the cellular isoform of the prion protein (PrP(C)) into the pathologic isoform (PrP(Sc)) is the key event in prion diseases. To study the conversion process, an in vitro system based on varying the concentration of low amounts of sodium dodecyl sulfate (SDS) has been employed. In the present study, t...

journal_title：Biological chemistry

authors： Stöhr J,Elfrink K,Weinmann N,Wille H,Willbold D,Birkmann E,Riesner D

更新日期：2011-05-01 00:00:00

abstract：:Nitrosative stress results from an increase in reactive nitrogen species (RNS) within the cell. Though the RNS - nitric oxide (·NO) and peroxynitrite (ONOO-) - play pivotal physiological roles, at elevated concentrations, these moieties can be poisonous to both prokaryotic and eukaryotic cells alike due to their capac...

journal_title：Biological chemistry

authors： Tharmalingam S,Alhasawi A,Appanna VP,Lemire J,Appanna VD

更新日期：2017-10-26 00:00:00

abstract：:Human kallikrein-related peptidases (KLKs), including the well-known prostate cancer biomarker, prostate-specific antigen (PSA-KLK3), along with 14 other serine proteinase KLK family members are now known to regulate cells by cleaving and activating members of the G-protein-coupled proteinase-activated receptor (PAR) ...

journal_title：Biological chemistry

authors： Hollenberg MD

更新日期：2014-09-01 00:00:00

abstract：:Adenoviral DNA was examined within capsids by dimethyl sulfate footprinting. Protein-DNA interactions were visualized through ligation-mediated PCR (LM-PCR). Signals for protein binding were found adjacent to both inverted terminal repeats (ITR). There were no indications of close protein binding at several other loci...

journal_title：Biological chemistry

authors： Dobner T,Büchner D,Zeller T,Wolf H,Niller HH

更新日期：2001-09-01 00:00:00

abstract：:Fanconi anemia is a fatal, hereditary chromosome instability syndrome of early childhood with progressive pancytopenia and cancer-proneness. Hypersensitivity to alkylating agents points to DNA repair inefficiency. Excess reactive oxygen intermediates and hypersensitivity to oxygen, all features of Fanconi anemia cells...

journal_title：Biological chemistry

authors： Kontou M,Hirsch-Kauffmann M,Schweiger M

更新日期：2008-10-01 00:00:00

abstract：:Transforming growth factor-beta1 (TGF-beta1) has a biphasic effect on the growth of renal epithelial cells. In transformed cells, TGF-beta1 appears to accelerate the proliferation of malignant cells. The diverse cellular functions of TGF-beta1 are regulated by three high-affinity serine/threonine kinase receptors, nam...

journal_title：Biological chemistry

authors： Moodley R,Snyman C,Odhav B,Bhoola KD

更新日期：2005-04-01 00:00:00

abstract：:Cathepsin E (CE) is an intracellular aspartic proteinase implicated in various physiological and pathological processes, yet its actual roles in vivo remain elusive. To assess the physiological significance of CE expression in tumor cells, human CE was stably expressed in human prostate carcinoma ALVA101 cells express...

journal_title：Biological chemistry

authors： Shin M,Kadowaki T,Iwata J,Kawakubo T,Yamaguchi N,Takii R,Tsukuba T,Yamamoto K

更新日期：2007-11-01 00:00:00

abstract：:Magnesium cation (Mg2+) is the most abundant divalent cation in living cells, where it is required for various intracellular functions. In chloroplasts and cyanobacteria, established photosynthetic model systems, Mg2+ is the central ion in chlorophylls, and Mg2+ flux across the thylakoid membrane is required for count...

journal_title：Biological chemistry

authors： Pohland AC,Schneider D

更新日期：2019-09-25 00:00:00

abstract：:Mammalian tyrosinases are key enzymes of melanin formation. Their native forms undergo complex maturation and sorting processes before being integrated into the melanosomal membrane, which greatly complicates their heterologous expression in other cell types. In the present work, we constructed several differently tru...

journal_title：Biological chemistry

authors： Cordes P,Sun W,Wolber R,Kolbe L,Klebe G,Röhm KH

更新日期：2013-05-01 00:00:00