Abstract:
:The BCL2 family of genes (B-cell CLL/lymphoma 2; Bcl-2) plays a pivotal role in the highly regulated process of apoptosis. We have recently cloned a newly identified member of this family, BCL2L12, which was found to be differentially expressed in many tumors. It is known that topotecan and methotrexate act through induction of apoptosis in cancer cells. In the present study we investigated the expression profile of the novel apoptotic gene BCL2L12 in relation to other apoptotic genes in the human leukemic cell line HL-60, after treatment with topotecan or methotrexate. The kinetics of apoptosis induction and cell toxicity were investigated by DNA laddering and the MTT method, respectively. Gene expression levels were analyzed by RT-PCR using gene-specific primers. Downregulation of BCL2L12, BCL2 and FAS was observed after treatment of HL-60 cells with topotecan, while treatment with methotrexate led to downregulation of BCL2 and FAS, with no change in BCL2L12 expression. Our results support the significance of mRNA modulations in the expression of apoptosis-related genes during treatment of human leukemic cells with anticancer drugs.
journal_name
Biol Chemjournal_title
Biological chemistryauthors
Floros KV,Talieri M,Scorilas Adoi
10.1515/BC.2006.203subject
Has Abstractpub_date
2006-12-01 00:00:00pages
1629-33issue
12eissn
1431-6730issn
1437-4315journal_volume
387pub_type
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journal_title:Biological chemistry
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pub_type: 杂志文章
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journal_title:Biological chemistry
pub_type: 杂志文章,评审
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doi:10.1515/BC.2008.035
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pub_type: 杂志文章
doi:10.1515/BC.2000.007
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pub_type: 杂志文章,评审
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更新日期:2004-11-01 00:00:00
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pub_type: 评论,杂志文章
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journal_title:Biological chemistry
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