Molecular recognition between Azotobacter vinelandii rhodanese and a sulfur acceptor protein.

abstract：:A water-soluble [18O]-labeled endoperoxide derived from N,N'-di(2,3-dihydroxypropyl)-1,4-naphthalene-dipropanamide (DHPN18O2) has been shown to act as a clean chemical source of [18O]-labeled molecular singlet oxygen. This allows the assessment of the singlet oxygen (1O2) reactivity toward biological targets such as D...

journal_title：Biological chemistry

authors： Martinez GR,Medeiros MH,Ravanat JL,Cadet J,Di Mascio P

更新日期：2002-03-01 00:00:00

abstract：:[NiFe] hydrogenases catalyze reversible hydrogen production/consumption. The active site of [NiFe] hydrogenases contains a complex NiFe(CN)2CO center, and the biosynthesis/maturation of these enzymes is a complex and dynamic process, primarily involving six Hyp proteins (HypABCDEF). HypA and HypB are involved in the N...

journal_title：Biological chemistry

authors： Watanabe S,Sasaki D,Tominaga T,Miki K

更新日期：2012-09-08 00:00:00

abstract：:A novel approach to studying the inter- and intrasubunit communication required for the activity of homodimeric proteins is described. It was developed for the restriction endonuclease EcoRV, but should also be useful for other homodimeric enzymes. Two ecorV genes encoding different EcoRV mutants are coexpressed in th...

journal_title：Biological chemistry

authors： Wende W,Stahl F,Pingoud A

更新日期：1996-10-01 00:00:00

abstract：:Molecular chaperones are well known as facilitators of protein folding and assembly. However, in recent years multiple chaperone-assisted degradation pathways have also emerged, including CAP (chaperone-assisted proteasomal degradation), CASA (chaperone-assisted selective autophagy), and CMA (chaperone-mediated autoph...

journal_title：Biological chemistry

authors： Kettern N,Dreiseidler M,Tawo R,Höhfeld J

更新日期：2010-05-01 00:00:00

abstract：:HB13 human myoblasts express physiological and biochemical markers associated with myoblast differentiation in non-human cell culture model systems. During differentiation, HB13 myoblasts also demonstrate fusion-related increases in cathepsin B activity and protein levels. These increases are associated with an increa...

journal_title：Biological chemistry

authors： Jane DT,Morvay LC,Koblinski J,Yan S,Saad FA,Sloane BF,Dufresne MJ

更新日期：2002-12-01 00:00:00

abstract：:Human inositol phosphate multikinase (IPMK) is a multifunctional protein in cellular signal transduction, namely, a multispecific inositol phosphate kinase, phosphatidylinositol 3-kinase, and a scaffold within the mTOR-raptor complex. To fulfill these nuclear and cytoplasmic functions, intracellular targeting of IPMK ...

journal_title：Biological chemistry

authors： Meyer R,Nalaskowski MM,Ehm P,Schröder C,Naj X,Brehm MA,Mayr GW

更新日期：2012-03-01 00:00:00

abstract：:We present a method that allows preparing long DNA containing defined mismatches without the use of gel electrophoretic or chromatographic purification steps. The preparation starts with the synthesis of two PCR products, which are identical except for those positions that will later form the mismatches. One of the PC...

journal_title：Biological chemistry

authors： Thomas E,Pingoud A,Friedhoff P

更新日期：2002-09-01 00:00:00

abstract：:The role of Zn2+-sensing receptor GPR39 on glucose homeostasis and incretin regulation was assessed in enteroendocrine L- and K-cells. Anti-hyperglycaemic, insulinotropic and incretin secreting properties of Zn2+ were explored in normal, diabetic and incretin receptor knockout mice. Compared to intraperitoneal injecti...

journal_title：Biological chemistry

authors： Moran BM,Miskelly MG,Abdel-Wahab YHA,Flatt PR,McKillop AM

更新日期：2019-06-20 00:00:00

abstract：:Parvulins belong to the family of peptidyl-prolyl cis/trans isomerases (PPIases) assisting in protein folding and in regulating the function of a broad variety of proteins in all branches of life. The human representatives Pin1 and Par14/17 are directly involved in processes influencing cellular maintenance and cell f...

journal_title：Biological chemistry

authors： Matena A,Rehic E,Hönig D,Kamba B,Bayer P

更新日期：2018-01-26 00:00:00

abstract：:Kaposi's sarcoma-associated herpesvirus (KSHV), or human herpesvirus 8 (HHV8) is a gammaherpesvirus and the etiological agent of Kaposi's sarcoma, primary effusion lymphoma and multicentric Castleman disease. The KSHV genome contains genes for a unique group of proteins with homology to cellular interferon regulatory ...

journal_title：Biological chemistry

authors： Koch S,Schulz TF

更新日期：2017-07-26 00:00:00

abstract：:Disruption of the plasma membrane poses deadly threat to eukaryotic cells and survival requires a rapid membrane repair system. Recent evidence reveal various plasma membrane repair mechanisms, which are required for cells to cope with membrane lesions including membrane fusion and replacement strategies, remodeling o...

journal_title：Biological chemistry

authors： Boye TL,Nylandsted J

更新日期：2016-10-01 00:00:00

abstract：:The sphingolipid sphingosine-1-phosphate (S1P) has various functions in immune cell biology, regulating survival, proliferation, and, most prominently, migration. S1P couples to five G protein-coupled receptors (S1PR1-5) to transduce its effects on immune cell function. Expression of S1PR4 is restricted to immune cell...

journal_title：Biological chemistry

authors： Dillmann C,Mora J,Olesch C,Brüne B,Weigert A

更新日期：2015-06-01 00:00:00

abstract：:Latent episomal genomes of Epstein-Barr virus, a human gammaherpesvirus, represent a suitable model system for studying replication and methylation of chromosomal DNA in mammals. We analyzed the methylation patterns of CpG dinucleotides in the latent origin of DNA replication of Epstein-Barr virus using automated fluo...

journal_title：Biological chemistry

authors： Salamon D,Takacs M,Myöhänen S,Marcsek Z,Berencsi G,Minarovits J

更新日期：2000-02-01 00:00:00

abstract：:SlyD is a bacterial two-domain protein that functions as a molecular chaperone, a prolyl cis/trans isomerase, and a nickel-binding protein. This review summarizes recent findings about the molecular enzyme mechanism of SlyD. The chaperone function located in one domain of SlyD is involved in twin-arginine translocatio...

journal_title：Biological chemistry

authors： Kovermann M,Schmid FX,Balbach J

更新日期：2013-08-01 00:00:00

abstract：:The production and processing of ribosomal RNA is a complex and well-coordinated nucleolar process for ribosome biogenesis. Progress in understanding nucleolar structure and function has lead to the unexpected discovery of the nucleolus as a highly sensitive sensor of cellular stress and an important regulator of the ...

journal_title：Biological chemistry

authors： Burger K,Eick D

更新日期：2013-09-01 00:00:00

abstract：:Tissue kallikrein KLK1 and the kallikrein-related peptidases KLK2-15 are a subfamily of serine proteases that have defined or proposed roles in a range of central nervous system (CNS) and non-CNS pathologies. To further understand their potential activity in multiple sclerosis (MS), serum levels of KLK1, 6, 7, 8 and 1...

journal_title：Biological chemistry

authors： Scarisbrick IA,Linbo R,Vandell AG,Keegan M,Blaber SI,Blaber M,Sneve D,Lucchinetti CF,Rodriguez M,Diamandis EP

更新日期：2008-06-01 00:00:00

abstract：:Bone morphogenetic proteins (BMP) are dimeric factors initiating several distinct signaling cascades by binding to two types of transmembrane serine/threonine kinase receptors (BRI and BRII), and are thus regulating several steps in embryonal development and adult tissue homeostasis. BMP-2 contains two symmetrical pai...

journal_title：Biological chemistry

authors： Knaus P,Sebald W

更新日期：2001-08-01 00:00:00

abstract：:We recently reported that human melanoma cells, but not benign melanocytes, aberrantly express kallikrein-related peptidase 7 (KLK7). Here, we show a KLK7 overexpression-mediated decrease of cell adhesion to extracellular matrix binding proteins, associated with downregulation of α5/β1/αv/β3 integrin expression. We al...

journal_title：Biological chemistry

authors： Haddada M,Draoui H,Deschamps L,Walker F,Delaunay T,Brattsand M,Magdolen V,Darmoul D

更新日期：2018-09-25 00:00:00

abstract：:DNA-based immunization has been shown to induce protective immunity against several microbial pathogens including HIV-1. Several routes of DNA vaccination have been exploited. However, the properties of the immune responses seem to differ with the different routes used for DNA delivery, ultimately affecting the outcom...

journal_title：Biological chemistry

authors： Asakura Y,Lundholm P,Kjerrström A,Benthin R,Lucht E,Fukushima J,Schwartz S,Okuda K,Wahren B,Hinkula J

更新日期：1999-03-01 00:00:00

abstract：:The purpose of this work was to study the regulative role of the glutamate receptor found earlier in the brain mitochondria. In the present work a glutamate-dependent signaling system with similar features was detected in mitochondria of the heart. The glutamate-dependent signaling system in the heart mitochondria was...

journal_title：Biological chemistry

authors： Selin AA,Lobysheva NV,Nesterov SV,Skorobogatova YA,Byvshev IM,Pavlik LL,Mikheeva IB,Moshkov DA,Yaguzhinsky LS,Nartsissov YR

更新日期：2016-05-01 00:00:00

abstract：:Glucose-dependent insulinotropic polypeptide (GIP) is an incretin hormone secreted by endocrine K-cells in response to nutrient absorption. In this study we have utilized a specific and enzymatically stable GIP receptor antagonist, (Pro3)GIP, to evaluate the contribution of endogenous GIP to insulin secretion and gluc...

journal_title：Biological chemistry

authors： Irwin N,Gault VA,Green BD,Greer B,McCluskey JT,Harriott P,O'Harte FP,Flatt PR

更新日期：2004-09-01 00:00:00

abstract：:Major advances have been made during the last decade in our understanding of adrenal steroid hormone biosynthesis. Two key players in these pathways are the human mitochondrial cytochrome P450 enzymes CYP11B1 and CYP11B2, which catalyze the final steps in the biosynthesis of cortisol and aldosterone. Using data from m...

journal_title：Biological chemistry

authors： Bureik M,Lisurek M,Bernhardt R

更新日期：2002-10-01 00:00:00

abstract：:The saline extract of Bauhinia bauhinioides dry seeds was shown to inhibit cruzipain, a cysteine proteinase from Trypanosoma cruzi. The inhibitory activity was assigned to a protein with 164 amino acid residues and molecular mass of 18 034 Da that was purified by chromatography on DEAE-Sephadex, trypsin-Sepharose (rem...

journal_title：Biological chemistry

authors： de Oliveira C,Santana LA,Carmona AK,Cezari MH,Sampaio MU,Sampaio CA,Oliva ML

更新日期：2001-05-01 00:00:00

abstract：:The p53 tumour suppressor gene is frequently mutated in human tumours and different tumour-derived mutations have varying effects on cells. The effect of a novel tumour-derived p53 mutation and two recently described mutations from South African breast cancer patients on the growth rate, colony formation, cell cycle a...

journal_title：Biological chemistry

authors： Donninger H,Binder A,Bohm L,Parker MI

更新日期：2008-01-01 00:00:00

abstract：:Type I allergy, a hypersensitivity disease affecting almost 20% of the population worldwide, is based on the IgE recognition of otherwise harmless antigens (i.e., allergens). Allergen-induced crosslink of effector cell-bound IgE antibodies leads to the release of biological mediators and thus to immediate disease symp...

journal_title：Biological chemistry

authors： Valenta R,Vrtala S,Focke-Tejkl M,Bugajska-Schretter,Ball T,Twardosz A,Spitzauer S,Grönlund H,Kraft D

更新日期：1999-07-01 00:00:00

abstract：:The HIRIP3 protein had been identified from its interaction with the HIRA histone chaperone. Experiments using anti-peptide antisera indicated that this 556-aa protein is nuclear throughout the cell cycle and excluded from condensed chromatin during mitosis. Based on its electrophoretic migration and sensitivity to ph...

journal_title：Biological chemistry

authors： Assrir N,Filhol O,Galisson F,Lipinski M

更新日期：2007-04-01 00:00:00

abstract：:Cellular volume regulation is fundamental for numerous physiological processes. The volume-regulated anion channel, VRAC, plays a crucial role in regulatory volume decrease. This channel, which is ubiquitously expressed in vertebrates, has been vastly characterized by electrophysiological means. It opens upon cell swe...

journal_title：Biological chemistry

authors： Stauber T

更新日期：2015-09-01 00:00:00

abstract：:Gaucher disease (GD) is a lysosomal storage disorder, caused by an impaired function of β-glucocerebrosidase, which results in accumulation of glucocerebroside in cells, and altered membrane ordering. Using electron paramagnetic resonance spin labeling, a statistically significant difference in the order parameter bet...

journal_title：Biological chemistry

authors： Pavićević A,Lakočević M,Popović M,Popović-Bijelić A,Daković M,Mojović M

更新日期：2018-04-25 00:00:00

abstract：:In preeclampsia and hemolysis, elevated liver enzymes and low platelet (HELLP) syndrome, impaired trophoblast invasion and excessive fibrin deposition in the placental intervillous space is associated with fetal compromise. However, little information is available whether modulation of placental protease expression--p...

journal_title：Biological chemistry

authors： von Steinburg SP,Krüger A,Fischer T,Mario Schneider KT,Schmitt M

更新日期：2009-11-01 00:00:00

abstract：:The influence of the cellular milieu, a complex and crowded solvent, is often neglected when biomolecular structure and function are studied in vitro. To mimic the cellular environment, crowding effects are commonly induced in vitro using artificial crowding agents like Ficoll or dextran. However, it is unclear if suc...

journal_title：Biological chemistry

authors： Gnutt D,Ebbinghaus S

更新日期：2016-01-01 00:00:00