Changes of the peripheral blood mononuclear cells membrane fluidity from type 1 Gaucher disease patients: an electron paramagnetic resonance study.

abstract：:DHX36 is a member of the DExD/H box helicase family, which comprises a large number of proteins involved in various cellular functions. Recently, the function of DHX36 in the regulation of G-quadruplexes (G4s) was demonstrated. G4s are alternative nucleic acid structures, which influence many cellular pathways on a tr...

journal_title：Biological chemistry

authors： Schult P,Paeschke K

更新日期：2020-10-01 00:00:00

abstract：:The periplasmic ligand-binding protein ChoX is part of the ABC transport system ChoVWX that imports choline as a nutrient into the soil bacterium Sinorhizobium meliloti. We have recently reported the crystal structures of ChoX in complex with its ligands choline and acetylcholine and the structure of a fully closed bu...

journal_title：Biological chemistry

authors： Oswald C,Smits SH,Höing M,Bremer E,Schmitt L

更新日期：2009-11-01 00:00:00

abstract：:We have previously shown that compatible organic osmolytes, such as betaine, myo-inositol and taurine, are part of the stress response of normal human keratinocytes (NHKs) to ultraviolet B (UVB) radiation. In this regard, we tested human HaCaT keratinocytes as a surrogate cell line for NHK. HaCaT cells osmo-dependentl...

journal_title：Biological chemistry

authors： Warskulat U,Brookmann S,Reinen A,Häussinger D

更新日期：2007-12-01 00:00:00

abstract：:The thyroglobulin type-1 (Tg-1) domain is a protein module that occurs in a variety of secreted and membrane proteins and is recognised as a potent inhibitor of cysteine peptidases. We present here some properties of the Tg-1 domain of human testican, a modularly organised proteoglycan secreted mainly by brain cells, ...

journal_title：Biological chemistry

authors： Meh P,Pavsic M,Turk V,Baici A,Lenarcic B

更新日期：2005-01-01 00:00:00

abstract：:Xeroderma pigmentosum complementation group D protein (XPD) is an iron-sulfur cluster containing 5'-3' helicase and, in humans, part of the transcription factor TFIIH. TFIIH is involved in nucleotide excision repair as well as in transcription initiation. Recently, three different groups have reported the structures o...

journal_title：Biological chemistry

authors： Wolski SC,Kuper J,Kisker C

更新日期：2010-07-01 00:00:00

abstract：:The cellular adhesion molecule E-selectin is expressed on activated endothelial cells, and is involved in the process of adherence of blood cells to vessel endothelium in inflammatory events such as atherosclerosis. In a recent study we found a Ser128Arg mutation in the EGF domain as well as a Leu554Phe mutation in th...

journal_title：Biological chemistry

authors： Wenzel K,Stahn R,Speer A,Denner K,Gläser C,Affeldt M,Moobed M,Scheer A,Baumann G,Felix SB

更新日期：1999-06-01 00:00:00

abstract：:Protein folding is one of the fundamental processes in life and therefore needs to be tightly regulated. Many cellular quality control systems are in place to ensure that proteostasis is optimally adjusted for a changing environment, facilitating protein folding, translocation and degradation. These systems include th...

journal_title：Biological chemistry

authors： Olzscha H

更新日期：2019-06-26 00:00:00

abstract：:In the SalI system, endonuclease activity can be only achieved in the presence of a functional modification gene. Thus, the DNA methyltransferase is involved in the control of restriction. By fusion of the restriction gene of the SalI system to the modification gene of the isospecific HgiDII system a hybrid type II re...

journal_title：Biological chemistry

authors： Pélaez AI,Ribas-Aparicio RM,Gómez A,Rodicio MR

更新日期：1998-04-01 00:00:00

abstract：:Low NO concentrations synthesized by constitutively expressed NO synthases act on several signaling pathways activating transcription factors (TF), such as NF-kappaB or AP-1, and thereby influence gene expression. In contrast, during inflammatory reactions the inducible NO synthase produces NO for prolonged periods of...

journal_title：Biological chemistry

authors： Kröncke KD

更新日期：2003-10-01 00:00:00

abstract：:We have investigated the methylation status of the repeat unit of the human ribosomal RNA genes in lung cancer. Using a Southern blot analysis approach we have determined that the non-transcribed region of these genes was generally heavily methylated, while the transcribed region was not methylated in either tumor or ...

journal_title：Biological chemistry

authors： Shiraishi M,Sekiguchi A,Chuu YH,Sekiya T

更新日期：1999-01-01 00:00:00

abstract：:92 kDa and 72 kDa gelatinases, two neutral proteinases exhibiting elastinolytic activity and secreted as zymogens by aortic smooth muscle cells, were shown to bind to insoluble elastin. The active form of each enzyme interacted with substrate more avidly than latent form. Once bound to insoluble elastin, 92 kDa progel...

journal_title：Biological chemistry

authors： Emonard H,Hornebeck W

更新日期：1997-03-01 00:00:00

abstract：:Neuritic plaques of Alzheimer patients are composed of multiple protein components. Among them, the amyloid beta-peptides (Abeta) 1-40/42 and further N- and C-terminally modified fragments of Abeta are highly abundant. Most prominent are the isoaspartate (isoAsp)-Abeta peptides and pyroglutamyl (pGlu)-Abeta. While pGl...

journal_title：Biological chemistry

authors： Böhme L,Hoffmann T,Manhart S,Wolf R,Demuth HU

更新日期：2008-08-01 00:00:00

abstract：:The lysosomal cysteine proteases cathepsin B and L play important roles in tumor cell invasion. An imbalance between these cathepsins and their endogenous inhibitors, the cystatins, has been associated with development of the metastatic phenotype. Accordingly, many studies have indicated potential use of cystatins in ...

journal_title：Biological chemistry

authors： Gianotti A,Sommer CA,Carmona AK,Henrique-Silva F

更新日期：2008-04-01 00:00:00

abstract：:Understanding the molecular mechanisms behind extreme temperature stability is of relevance for the protein folding problem and for designing proteins for industrial and medical applications. A powerful approach for understanding the structural basis of thermostability is the comparison of high resolution structures o...

journal_title：Biological chemistry

authors： Macedo-Ribeiro S,Darimont B,Sterner R

更新日期：1997-03-01 00:00:00

abstract：:Many neurodegenerative disorders and syndromes are associated with an excessive generation of reactive oxygen species (ROS) and oxidative stress. The pathways to nerve cell death induced by diverse potential neurotoxins such as peptides, excitatory amino acids, cytokines or synthetic drugs commonly share oxidative dow...

journal_title：Biological chemistry

authors： Behl C,Moosmann B

更新日期：2002-03-01 00:00:00

abstract：:We studied the mode of regulation of the activity of mature cathepsin B (CB) by L-cysteine and some related thiols. The activity of CB with Z-Arg-Arg-NHMec as substrate was gradually inhibited over a range of increasing concentration of Cys, Cys methyl ester (CysOMe), Cys ethyl ester (CysOEt), N-acetyl-Cys (N-AcCys) a...

journal_title：Biological chemistry

authors： Krepela E,Procházka J,Kárová B

更新日期：1999-05-01 00:00:00

abstract：:As an important protein, UL31 has been demonstrated to play multiple roles in herpes simplex virus 1 (HSV-1) replication. Previous studies showed that UL31 predominantly locates in the nucleus in chemical fixed cells and live cells, however, the determining mechanisms for its nuclear translocation is not clear. In the...

journal_title：Biological chemistry

authors： Cai M,Si J,Li X,Zeng Z,Li M

更新日期：2016-06-01 00:00:00

abstract：:MHC (major histocompatibility complex) class I molecules bind intracellular virus-derived peptides in the endoplasmic reticulum (ER) and present them at the cell surface to cytotoxic T lymphocytes. Peptide-free class I molecules at the cell surface, however, could lead to aberrant T cell killing. Therefore, cells ensu...

journal_title：Biological chemistry

authors： Wright CA,Kozik P,Zacharias M,Springer S

更新日期：2004-09-01 00:00:00

abstract：:Abstract Fanconi anemia (FA) is a rare cancer-prone genetic disorder characterized by progressive bone marrow failure, chromosomal instability and redox abnormalities. There is much biochemical and genetic data, which strongly suggest that FA cells experience increased oxidative stress. The present study was designed ...

journal_title：Biological chemistry

authors： Petrovic S,Leskovac A,Kotur-Stevuljevic J,Joksic J,Guc-Scekic M,Vujic D,Joksic G

更新日期：2011-07-01 00:00:00

abstract：:RIO1 is an essential gene that encodes a protein serine kinase and is transcribed constitutively at a very low level. Transcriptional activation of RIO1 dispenses with a canonical TATA box as well as with classical transactivators or specific DNA-binding factors. Instead, a dG-dC-rich sequence element, that is located...

journal_title：Biological chemistry

authors： Angermayr M,Schwerdffeger K,Bandlow W

更新日期：2003-09-01 00:00:00

abstract：:Vaccination with either exogenous hepatitis B surface antigen (HBsAg) lipoprotein particles without adjuvants, or plasmid DNA encoding secreted small HBsAg stimulate long-lasting, potent antibody responses in H-2d (BALB/c) and C57Bl/6 (H-2b) mice. Vaccination with exogenous HBsAg primes MHC-I restricted cytotoxic T ly...

journal_title：Biological chemistry

authors： Schirmbeck R,Reimann J

更新日期：1999-03-01 00:00:00

abstract：:Five moeA mutants were generated by replacing some conserved amino acids of MoeA by site-directed mutagenesis. The mutants were assayed for the ability to restore in vivo nitrate reductase activity of the moeA mutant Escherichia coli JRG97 and in vitro Neurospora crassa nit-1 nitrate reductase activity. The replacemen...

journal_title：Biological chemistry

authors： Sandu C,Brandsch R

更新日期：2002-02-01 00:00:00

abstract：:Abstract The R- and K-gingipain proteases of Porphyromonas gingivalis are involved in proteolysis of haemoglobin from which the defensive dimeric haem pigment is formed. Whilst oxyhaemoglobin is refractory towards K-gingipain, methaemoglobin is rapidly degraded. Ligation of methaemoglobin with N3-, which effectively b...

journal_title：Biological chemistry

authors： Smalley JW,Birss AJ,Szmigielski B,Potempa J

更新日期：2008-09-01 00:00:00

abstract：:Transcriptional enhancers are short (200-1500 base pairs) DNA segments that are able to dramatically boost transcription from the promoter of a target gene. Originally discovered in simian virus 40 (SV40), a small DNA virus, transcription enhancers were soon also found in immunoglobulin genes and other cellular genes ...

journal_title：Biological chemistry

authors： Schaffner W

更新日期：2015-04-01 00:00:00

abstract：:The BCL2 family of genes (B-cell CLL/lymphoma 2; Bcl-2) plays a pivotal role in the highly regulated process of apoptosis. We have recently cloned a newly identified member of this family, BCL2L12, which was found to be differentially expressed in many tumors. It is known that topotecan and methotrexate act through in...

journal_title：Biological chemistry

authors： Floros KV,Talieri M,Scorilas A

更新日期：2006-12-01 00:00:00

abstract：:Insulin contains three disulfide bonds, one intrachain bond, A6-A11, and two interchain bonds, A7-B7 and A20-B19. Site-directed mutagenesis results (the two cysteine residues of disulfide A7-B7 were replaced by serine) showed that disulfide A7-B7 is crucial to both the structure and activity of insulin. However, chemi...

journal_title：Biological chemistry

authors： Guo ZY,Jia XY,Feng YM

更新日期：2004-12-01 00:00:00

abstract：:Structural and functional characterization of integral membrane proteins in a bilayer environment is strongly hampered by the requirement of detergents for solubilization and subsequent purification, as detergents commonly affect their structure and/or activity. Here, we describe a rapid procedure with minimal exposur...

journal_title：Biological chemistry

authors： Shirzad-Wasei N,van Oostrum J,Bovee-Geurts PH,Kusters LJ,Bosman GJ,DeGrip WJ

更新日期：2015-08-01 00:00:00

abstract：:The vast majority of malignancies detected in renal parenchyma are diagnosed as renal cell carcinoma (RCC), whose subtype discrimination and determination of prognosis may contribute to the selection of the adequate therapy. Recently, a new class of small non-coding RNAs, known as microRNAs, has proven to be among the...

journal_title：Biological chemistry

authors： Papadopoulos EI,Petraki C,Gregorakis A,Fragoulis EG,Scorilas A

更新日期：2016-06-01 00:00:00

abstract：:The ubiquitous calpains, mu- and m-calpain, have been implicated in essential physiological processes and various pathologies. Cell-permeable specific inhibitors are important tools to elucidate the roles of calpains in cultivated cells and animal models. The synthetic N-acetylated 27-mer peptide derived from exon B o...

journal_title：Biological chemistry

authors： Gil-Parrado S,Assfalg-Machleidt I,Fiorino F,Deluca D,Pfeiler D,Schaschke N,Moroder L,Machleidt W

更新日期：2003-03-01 00:00:00

abstract：:During the last decade, an increasing number of new enzymes containing glycyl radicals in their active sites have been identified and biochemically characterised. These include benzylsuccinate synthase (Bss), 4-hydroxyphenylacetate decarboxylase (Hpd) and the coenzyme B12-independent glycerol dehydratase (Gdh). These ...

journal_title：Biological chemistry

authors： Selmer T,Pierik AJ,Heider J

更新日期：2005-10-01 00:00:00