Functional ribosome biogenesis is a prerequisite for p53 destabilization: impact of chemotherapy on nucleolar functions and RNA metabolism.

abstract：:Only one transcript for KLK10 was identified by RT-PCR in lung tissue, whereas KLK11 expressed at least four alternative transcripts. Quantitative analysis of KLK10 and KLK11 expression levels was assessed by real-time PCR, in a cohort of 47 patients with non-small-cell lung cancer (NSCLC). Expression levels of these ...

journal_title：Biological chemistry

authors： Planque C,Aïnciburu M,Heuzé-Vourc'h N,Régina S,de Monte M,Courty Y

更新日期：2006-06-01 00:00:00

abstract：:Amphetamine ('Speed'), methamphetamine ('Ice') and its congener 3,4-methylenedioxymethamphetamine (MDMA; 'Ecstasy') are illicit drugs abused worldwide for their euphoric and stimulant effects. Despite compelling evidence for chronic MDMA neurotoxicity in animal models, the physiological consequences of such toxicity i...

journal_title：Biological chemistry

authors： Steinkellner T,Freissmuth M,Sitte HH,Montgomery T

更新日期：2011-01-01 00:00:00

abstract：:A kinetic study was performed of a model for an autocatalytic zymogen activation process involving both intra- and intermolecular routes, to which a chromogenic reaction in which the active enzyme acts upon one of its substrates was coupled to continuously monitor the reaction. Kinetic equations describing the evoluti...

journal_title：Biological chemistry

authors： Fuentes ME,Varón R,García-Moreno M,Valero E

更新日期：2005-07-01 00:00:00

abstract：:An inert polysaccharide scaffold identified as a 5-15% component of prion rods (PrP 27-30) is unambiguously distinguishable from the N-glycosyl groups and the GPI anchor of PrP, and consists predominantly of 1,4-linked glucose with some branching via 1,4,6-linked glucose. We show that this polysaccharide scaffold is a...

journal_title：Biological chemistry

authors： Dumpitak C,Beekes M,Weinmann N,Metzger S,Winklhofer KF,Tatzelt J,Riesner D

更新日期：2005-11-01 00:00:00

abstract：:The evolution of a highly developed nervous system is mirrored by the ability of individual neurons to develop increased morphological complexity. As microtubules (MTs) are crucially involved in neuronal development, we tested the hypothesis that the evolution of complexity is driven by an increasing capacity of the M...

journal_title：Biological chemistry

authors： Trushina NI,Mulkidjanian AY,Brandt R

更新日期：2019-08-27 00:00:00

abstract：:In this work we demonstrate that heat and pressure induce only slightly different energetic changes in the unfolded state of RNase A. Using pressure and temperature as denaturants on a significant number of variants, and by determining the free energy of unfolding at different temperatures, we estimated the stability ...

journal_title：Biological chemistry

authors： Font J,Benito A,Torrent J,Lange R,Ribó M,Vilanova M

更新日期：2006-03-01 00:00:00

abstract：:Angiotensin-converting enzyme (ACE) plays an important role in blood pressure control. ACE also has effects on renal function, reproduction, hematopoiesis, and several aspects of the immune response. ACE 10/10 mice overexpress ACE in monocytic cells; macrophages from ACE 10/10 mice demonstrate increased polarization t...

journal_title：Biological chemistry

authors： Bernstein KE,Gonzalez-Villalobos RA,Giani JF,Shah K,Bernstein E,Janjulia T,Koronyo Y,Shi PD,Koronyo-Hamaoui M,Fuchs S,Shen XZ

更新日期：2014-10-01 00:00:00

abstract：:Human kallikrein-related peptidases (KLKs), including the well-known prostate cancer biomarker, prostate-specific antigen (PSA-KLK3), along with 14 other serine proteinase KLK family members are now known to regulate cells by cleaving and activating members of the G-protein-coupled proteinase-activated receptor (PAR) ...

journal_title：Biological chemistry

authors： Hollenberg MD

更新日期：2014-09-01 00:00:00

abstract：:Heparan sulfate proteoglycans are obligatory for receptor binding and mitogenic activity of the basic fibroblast growth factor (bFGF). In the present study the influence of undersulfated heparan sulfate on the expression of basic fibroblast growth factor and coronary smooth muscle cell (cSMC) proliferation was investi...

journal_title：Biological chemistry

authors： Schriever C,Breithardt G,Schmidt A

更新日期：1997-07-01 00:00:00

abstract：:Eremophilanes are sesquiterpenes with a rearranged carbon skeleton formed both by plants and fungi, however, almost no plant eremophilanes are found in fungi. These eremophilanes possess mainly phytotoxic, antimicrobial, anticancer and immunomodulatory properties and in this review fungal eremophilanes with bioactivit...

journal_title：Biological chemistry

authors： Yuyama KT,Fortkamp D,Abraham WR

更新日期：2017-12-20 00:00:00

abstract：:The influence of osmolarity and compatible organic osmolytes on the phosphorylation of the MAP-kinases Erk-1 and Erk-2 and on the expression of taurine transporter (TAUT) and lipopolysaccharide (LPS)-induced nitric oxide synthetase (iNOS) was studied in RAW 264.7 mouse macrophages. Hypoosmolarity (205 mosmol/l) but no...

journal_title：Biological chemistry

authors： Warskulat U,Schliess F,Häussinger D

更新日期：1998-07-01 00:00:00

abstract：:Seven purified proteins may be combined to reconstitute regulated, promoter-dependent RNA polymerase II transcription: five general transcription factors, Mediator, and RNA polymerase II. The entire system has been conserved across species from yeast to humans. The structure of RNA polymerase II, consisting of 10 poly...

journal_title：Biological chemistry

authors： Kornberg RD

更新日期：2001-08-01 00:00:00

abstract：:SlyD is a bacterial two-domain protein that functions as a molecular chaperone, a prolyl cis/trans isomerase, and a nickel-binding protein. This review summarizes recent findings about the molecular enzyme mechanism of SlyD. The chaperone function located in one domain of SlyD is involved in twin-arginine translocatio...

journal_title：Biological chemistry

authors： Kovermann M,Schmid FX,Balbach J

更新日期：2013-08-01 00:00:00

abstract：:Full-length Hsp70 protein (Hsp70) and the C-terminal domain of Hsp70 (Hsp70C) both stimulate the cytolytic activity of naive natural killer (NK) cells against Hsp70-positive tumor target cells. Here, we describe the characterization of Hsp70-NK cell interaction with binding studies using the human NK cell line YT. Bin...

journal_title：Biological chemistry

authors： Gross C,Hansch D,Gastpar R,Multhoff G

更新日期：2003-02-01 00:00:00

abstract：:Small-angle scattering (SAS) of X-rays and neutrons reveals low-resolution structures of biological macromolecules in solution. With the recent experimental and methodological advances, SAS became a unique tool for characterising biological systems. The method covers an extremely broad range of molecule sizes (from a ...

journal_title：Biological chemistry

authors： Svergun DI

更新日期：2010-07-01 00:00:00

abstract：:As a crucial protein, the herpes simplex virus 1 (HSV-1) UL2 protein has been shown to take part in various stages of viral infection, nonetheless, its exact subcellular localization and transport molecular determinants are not well known thus far. In the present study, by using live cells fluorescent microscopy assay...

journal_title：Biological chemistry

authors： Cai M,Huang Z,Liao Z,Chen T,Wang P,Jiang S,Chen D,Peng T,Bian Y,Hong G,Yang H,Zeng Z,Li X,Li M

更新日期：2017-04-01 00:00:00

abstract：:The solute carrier family SLC10 consists of seven members, including the bile acid transporters Na+/taurocholate co-transporting polypeptide (NTCP) and apical sodium-dependent bile acid transporter (ASBT), the steroid sulfate transporter SOAT as well as four orphan carriers (SLC10A3, SLC10A4, SLC10A5 and SLC10A7). Pre...

journal_title：Biological chemistry

authors： Noppes S,Müller SF,Bennien J,Holtemeyer M,Palatini M,Leidolf R,Alber J,Geyer J

更新日期：2019-09-25 00:00:00

abstract：:Protein disulfide isomerase (PDI) is a protein of the endoplasmic reticulum (ER) that is essential for the unscrambling of nonnative disulfide bonds. Here, we have determined the importance of PDI to both spore germination and vegetative cell division. To vary the concentration of PDI in the ER, we used plasmids that ...

journal_title：Biological chemistry

authors： Laboissière MC,Sturley SL,Raines RT

更新日期：1997-05-01 00:00:00

abstract：:It is now appreciated that mitochondria form tubular networks that adapt to the requirements of the cell by undergoing changes in their shape through fission and fusion. Proper mitochondrial distribution also appears to be required for ATP delivery and calcium regulation, and, in some cases, for cell development. Whil...

journal_title：Biological chemistry

authors： Frazier AE,Kiu C,Stojanovski D,Hoogenraad NJ,Ryan MT

更新日期：2006-12-01 00:00:00

abstract：:Recent progress in the identification and partial characterization of novel genes encoding cysteine proteases of the papain family has considerably increased our knowledge of this family of enzymes. Kinetic data available to date for this large family indicate relatively broad, overlapping specificities for most enzym...

journal_title：Biological chemistry

authors： Nägler DK,Ménard R

更新日期：2003-06-01 00:00:00

abstract：:Magnesium cation (Mg2+) is the most abundant divalent cation in living cells, where it is required for various intracellular functions. In chloroplasts and cyanobacteria, established photosynthetic model systems, Mg2+ is the central ion in chlorophylls, and Mg2+ flux across the thylakoid membrane is required for count...

journal_title：Biological chemistry

authors： Pohland AC,Schneider D

更新日期：2019-09-25 00:00:00

abstract：:Two current hypotheses of O2 sensing in the carotid body (CB) chemoreceptors suggest participation of oxygen reactive (ROS) species, but they are mechanistically opposed. One postulates that hypoxia decreases ROS levels; the other that hypoxia increases them. Yet, both propose that the ensuing alteration in the cellul...

journal_title：Biological chemistry

authors： Gonzalez C,Sanz-Alyayate G,Agapito MT,Obeso A

更新日期：2004-03-01 00:00:00

abstract：:The O-acetylation of sialic acids turns out to be one of the most important modifications that influence the diverse biological and pathophysiological properties of glycoconjugates in animals and microorganisms. To understand the functions of this esterification, knowledge of the properties, structures and regulation ...

journal_title：Biological chemistry

authors： Iwersen M,Dora H,Kohla G,Gasa S,Schauer R

更新日期：2003-07-01 00:00:00

abstract：:Mitochondria are indispensable cellular organelles providing ATP and numerous other essential metabolites to ensure cell survival. Reactive oxygen species (ROS), which are formed as side reactions during oxidative phosphorylation or by external agents, induce molecular damage in mitochondrial proteins, lipids/membrane...

journal_title：Biological chemistry

authors： Zimmermann M,Reichert AS

更新日期：2017-12-20 00:00:00

abstract：:The flagellate Euglena gracilis contains as photoreceptor complex a heterotetrameric light-sensitive adenylyl cyclase (AC), consisting of the flavoproteins PACalpha and PACbeta. Previously, we demonstrated the functional expression of PACalpha and PACbeta in oocytes from Xenopus laevis and of PACalpha in different ani...

journal_title：Biological chemistry

authors： Looser J,Schröder-Lang S,Hegemann P,Nagel G

更新日期：2009-11-01 00:00:00

abstract：:A putative operon of the ribosomal proteins S20 and Thx has been determined in a 1.4 kb sequenced region of T. thermophilus genomic DNA. Both genes have a promoter sequence 29 nt upstream of ORF1, possess their own Shine-Dalgarno motifs (GGAG) and are separated by only 9 nucleotides, a feature characteristic of the co...

journal_title：Biological chemistry

authors： Leontiadou F,Triantafillidou D,Choli-Papadopoulou T

更新日期：2001-07-01 00:00:00

abstract：:Nitrogen dioxide (*NO2) is an oxidizing free radical which can initiate a variety of destructive pathways in living systems, and several diseases are suspected to be connected with both exogenously and endogenously formed *NO2. Peroxynitrite (ONOO-/ONOOH) is believed to be an important endogenous source of *NO2 radica...

journal_title：Biological chemistry

authors： Kirsch M,Korth HG,Sustmann R,de Groot H

更新日期：2002-03-01 00:00:00

abstract：:FK506-binding proteins (FKBPs) belong to a distinct class of immunophilins that interact with immunosuppressants. They use their peptidyl-prolyl isomerase (PPIase) activity to catalyze the cis-trans conversion of prolyl bonds in proteins during protein-folding events. FKBPs also act as a unique group of chaperones. Th...

journal_title：Biological chemistry

authors： Orłowski M,Popławska K,Pieprzyk J,Szczygieł-Sommer A,Więch A,Zarębski M,Tarczewska A,Dobrucki J,Ożyhar A

更新日期：2018-04-25 00:00:00

abstract：:Mutations of the p53 gene may alter the specific regulatory domains of the protein. We examined the conserved domains III, IV and V by SSCP using PCR primers covering exons 5, 6, 7 and 8 from hairy cell leukemia (HCL), polyps, colorectal and gastric carcinomas. A low rate of p53 mutations was detected in HCL and polyp...

journal_title：Biological chemistry

authors： Marcsek ZL,König EA

更新日期：1998-04-01 00:00:00

abstract：:Recombinant expression of actin in bacteria results in non-native species that aggregate into inclusion bodies. Actin is a folding substrate of TRiC, the chaperonin of the eukaryotic cytosol. By employing bacterial in vitro translation lysates supplemented with purified chaperones, we have found that TRiC is the only ...

journal_title：Biological chemistry

authors： Stemp MJ,Guha S,Hartl FU,Barral JM

更新日期：2005-08-01 00:00:00