Small-angle X-ray and neutron scattering as a tool for structural systems biology.

abstract：:The saline extract of Bauhinia bauhinioides dry seeds was shown to inhibit cruzipain, a cysteine proteinase from Trypanosoma cruzi. The inhibitory activity was assigned to a protein with 164 amino acid residues and molecular mass of 18 034 Da that was purified by chromatography on DEAE-Sephadex, trypsin-Sepharose (rem...

journal_title：Biological chemistry

authors： de Oliveira C,Santana LA,Carmona AK,Cezari MH,Sampaio MU,Sampaio CA,Oliva ML

更新日期：2001-05-01 00:00:00

abstract：:The bifunctional protein DCoH (dimerization cofactor of HNF-1) is a transcriptional coactivator of homeodomain proteins and a cytoplasmic enzyme. Here we report on the isolation, nucleotide sequence and genomic structure of the chicken and rat DCoH genes. They have an unusual first exon coding for a single amino acid....

journal_title：Biological chemistry

authors： Bossow S,Riepl S,Igo-Kemenes T

更新日期：1998-11-01 00:00:00

abstract：:Fibroblast growth factor (FGF) receptor substrate 2 (FRS2) is a membrane-anchored docking protein that has been shown to play an important role in linking FGF, nerve growth factor (NGF) and glial cell-derived neurotrophic factor (GDNF) receptors with the Ras/mitogen-activated protein (MAP) kinase signaling cascade. He...

journal_title：Biological chemistry

authors： Wu Y,Chen Z,Ullrich A

更新日期：2003-08-01 00:00:00

abstract：:Parvulins belong to the family of peptidyl-prolyl cis/trans isomerases (PPIases) assisting in protein folding and in regulating the function of a broad variety of proteins in all branches of life. The human representatives Pin1 and Par14/17 are directly involved in processes influencing cellular maintenance and cell f...

journal_title：Biological chemistry

authors： Matena A,Rehic E,Hönig D,Kamba B,Bayer P

更新日期：2018-01-26 00:00:00

abstract：:The transcription of the fatty acid synthase (FAS) gene is regulated by the sterol status of the cell via cleavage of the sterol regulatory element-binding protein (SREBP). When human HepG2 hepatoma cells were cotransfected with an expression plasmid for mature SREBP-1a together with FAS promoter/reporter constructs s...

journal_title：Biological chemistry

authors： Wolf SS,Roder K,Sickinger S,Schweizer M

更新日期：2001-07-01 00:00:00

abstract：:Abstract The homeostasis and atheroprotective function of bilirubin could be an appealing model to investigate one of the many physiologic functions of the human aryl hydrocarbon receptor (AhR). Several clinical and epidemiological studies have been carried out on key enzymes generating and eliminating bilirubin (heme...

journal_title：Biological chemistry

authors： Bock KW,Köhle C

更新日期：2010-06-01 00:00:00

abstract：:Transcriptional enhancers are short (200-1500 base pairs) DNA segments that are able to dramatically boost transcription from the promoter of a target gene. Originally discovered in simian virus 40 (SV40), a small DNA virus, transcription enhancers were soon also found in immunoglobulin genes and other cellular genes ...

journal_title：Biological chemistry

authors： Schaffner W

更新日期：2015-04-01 00:00:00

abstract：:In mammalian cells, glucosylceramide (GlcCer), the simplest glycosphingolipid, is hydrolyzed by the lysosomal enzyme acid beta-glucosidase (GlcCerase). In the human metabolic disorder Gaucher disease, GlcCerase activity is significantly decreased owing to one of approximately 200 mutations in the GlcCerase gene. The m...

journal_title：Biological chemistry

authors： Kacher Y,Brumshtein B,Boldin-Adamsky S,Toker L,Shainskaya A,Silman I,Sussman JL,Futerman AH

更新日期：2008-11-01 00:00:00

abstract：:This article presents a historical account of the discovery of reversible protein phosphorylation. The process was uncovered in the mid-1950s in a study designed to elucidate the complex hormonal regulation of skeletal muscle glycogen phosphorylase. This enzyme was originally thought to be regulated by AMP now known t...

journal_title：Biological chemistry

authors： Fischer EH

更新日期：2010-02-01 00:00:00

abstract：:DEAD-box helicases catalyze RNA duplex unwinding in an ATP-dependent reaction. Members of the DEAD-box helicase family consist of a common helicase core formed by two RecA-like domains. According to the current mechanistic model for DEAD-box mediated RNA unwinding, binding of RNA and ATP triggers a conformational chan...

journal_title：Biological chemistry

authors： Rudolph MG,Klostermeier D

更新日期：2015-08-01 00:00:00

abstract：:The expression of the cysteine protease cathepsin B is increased in early stages of human breast cancer.To assess the potential role of cathepsin B in premalignant progression of breast epithelial cells, we employed a 3D reconstituted basement membrane overlay culture model of MCF10A human breast epithelial cells and ...

journal_title：Biological chemistry

authors： Mullins SR,Sameni M,Blum G,Bogyo M,Sloane BF,Moin K

更新日期：2012-12-01 00:00:00

abstract：:Few therapeutic options are offered to treat inflammation and alveolar wall destruction in emphysema. The effect of recombinant human pre-elafin, an elastase inhibitor, was evaluated in porcine pancreatic elastase (PPE)-induced emphysema in C57BL/6 mice. In a first protocol, mice received a single instillation of pre-...

journal_title：Biological chemistry

authors： Janelle MF,Doucet A,Bouchard D,Bourbonnais Y,Tremblay GM

更新日期：2006-07-01 00:00:00

abstract：:Maintenance of cellular redox control is pivotal for normal cellular functions and cell fate decisions including cell death. Among the key cellular redox systems in mammals, the glutathione peroxidase (GPX) family of proteins is the largest conferring multifaceted functions and affecting virtually all cellular process...

journal_title：Biological chemistry

authors： Buday K,Conrad M

更新日期：2020-10-22 00:00:00

abstract：:Metachromatic leukodystrophy is a lysosomal disorder caused by the deficiency of arylsulfatase A (ASA). This leads to the storage of the sphingolipid 3-O-sulfogalactosylceramide (sulfatide) in various cell types, such as renal tubular cells. Examination of mannose 6-phosphate receptor (MPR300)-dependent endocytosis re...

journal_title：Biological chemistry

authors： Klein D,Yaghootfam A,Matzner U,Koch B,Braulke T,Gieselmann V

更新日期：2009-01-01 00:00:00

abstract：:Annexins are a highly conserved protein family that bind to phospholipids in a calcium (Ca2+) - dependent manner. Studies with purified annexins, as well as overexpression and knockdown approaches identified multiple functions predominantly linked to their dynamic and reversible membrane binding behavior. However, mos...

journal_title：Biological chemistry

authors： Grewal T,Wason SJ,Enrich C,Rentero C

更新日期：2016-10-01 00:00:00

abstract：:Interleukin-13 (IL-13) is a T-cell-derived pleiotropic cytokine of particular medical importance because of its critical role in the development of allergic asthma. The effects of IL-13 on its target cells are mediated through a dimeric transmembrane receptor (IL-13R), which shares the IL-4Ralpha subunit with the IL-4...

journal_title：Biological chemistry

authors： Knackmuss S,Krause S,Engel K,Reusch U,Virchow JC,Mueller T,Kraich M,Little M,Luttmann W,Friedrich K

更新日期：2007-03-01 00:00:00

abstract：:In preeclampsia and hemolysis, elevated liver enzymes and low platelet (HELLP) syndrome, impaired trophoblast invasion and excessive fibrin deposition in the placental intervillous space is associated with fetal compromise. However, little information is available whether modulation of placental protease expression--p...

journal_title：Biological chemistry

authors： von Steinburg SP,Krüger A,Fischer T,Mario Schneider KT,Schmitt M

更新日期：2009-11-01 00:00:00

abstract：:The albumin gene family arose in a series of duplication events which gave rise to symmetry in its structure. The four genes are tandemly linked on human chromosome 4q in the order: 5'ALB-5'AFP-5'ALF-5'DBP-centromere, and their introns display a symmetrical and repetitive pattern that is shared by members of the gene ...

journal_title：Biological chemistry

authors： Nishio H,Hamdi HK,Dugaiczyk A

更新日期：1999-12-01 00:00:00

abstract：:Insulin contains three disulfide bonds, one intrachain bond, A6-A11, and two interchain bonds, A7-B7 and A20-B19. Site-directed mutagenesis results (the two cysteine residues of disulfide A7-B7 were replaced by serine) showed that disulfide A7-B7 is crucial to both the structure and activity of insulin. However, chemi...

journal_title：Biological chemistry

authors： Guo ZY,Jia XY,Feng YM

更新日期：2004-12-01 00:00:00

abstract：:Historically considered as accessory cells to neurons, there is an increasing interest in the role of astrocytes in normal and pathological conditions. Astrocytes are involved in neurotransmitter recycling, antioxidant supply, ion buffering and neuroinflammation, i.e. a lot of the same pathways that go astray in Alzhe...

journal_title：Biological chemistry

authors： Zulfiqar S,Garg P,Nieweg K

更新日期：2019-08-27 00:00:00

abstract：:The multifunctional helper component proteinase (HC-Pro) of potyviruses contains an autoproteolytic function that, together with the protein 1 (P1) and NIa proteinase, processes the polyprotein into mature proteins. In this study, we analysed the autoproteolytic active domain of zucchini yellow mosaic virus (ZYMV) HC-...

journal_title：Biological chemistry

authors： Boonrod K,Füllgrabe MW,Krczal G,Wassenegger M

更新日期：2011-10-01 00:00:00

abstract：:The HIRIP3 protein had been identified from its interaction with the HIRA histone chaperone. Experiments using anti-peptide antisera indicated that this 556-aa protein is nuclear throughout the cell cycle and excluded from condensed chromatin during mitosis. Based on its electrophoretic migration and sensitivity to ph...

journal_title：Biological chemistry

authors： Assrir N,Filhol O,Galisson F,Lipinski M

更新日期：2007-04-01 00:00:00

abstract：:Alternative splicing is a process by which multiple messenger RNAs (mRNAs) are generated from a single pre-mRNA, resulting in functionally distinct protein products. This is accomplished by the differential recognition of splice sites in the pre-mRNA, often regulated in a tissue- or development-specific manner. Altern...

journal_title：Biological chemistry

authors： Hui J,Bindereif A

更新日期：2005-12-01 00:00:00

abstract：:Ammonia is a major player in the pathogenesis of hepatic encephalopathy (HE) and affects astrocyte function by triggering a self-amplifying cycle between osmotic and oxidative stress. We recently demonstrated that hypoosmotic astrocyte swelling rapidly stimulates nitric oxide (NO) production and increases intracellula...

journal_title：Biological chemistry

authors： Kruczek C,Görg B,Keitel V,Bidmon HJ,Schliess F,Häussinger D

更新日期：2011-12-01 00:00:00

abstract：:The mitochondrial DNA (mtDNA) encompasses two classes of functionally important sequence variants: recent pathogenic mutations and ancient adaptive polymorphisms. To rapidly and cheaply evaluate both classes of single nucleotide variants (SNVs), we have developed an integrated system in which mtDNA SNVs are analyzed b...

journal_title：Biological chemistry

authors： Poole JC,Procaccio V,Brandon MC,Merrick G,Wallace DC

更新日期：2010-10-01 00:00:00

abstract：:The influence of the cellular milieu, a complex and crowded solvent, is often neglected when biomolecular structure and function are studied in vitro. To mimic the cellular environment, crowding effects are commonly induced in vitro using artificial crowding agents like Ficoll or dextran. However, it is unclear if suc...

journal_title：Biological chemistry

authors： Gnutt D,Ebbinghaus S

更新日期：2016-01-01 00:00:00

abstract：:In a recent study we showed that two proteinases (CMS2MS2 and CMS2MS3) from Carica candamarcensis enhance mammalian cell proliferation. The aim of the present study is the determination of the primary structure of CMS2MS2 and prediction of its three-dimensional structure. The protein contains 214 residues, including t...

journal_title：Biological chemistry

authors： Gomes MT,Bemquerer MP,Lopes MT,Richardson M,Júnior SO,Salas CE

更新日期：2007-08-01 00:00:00

abstract：:Myocardial zinc dyshomeostasis is associated with caspase-3 activation, ErbB2 degradation and apoptosis during hypoxia/reoxygenation. Zinc pyrithione replenishes intracellular zinc, suppresses caspase-3, augments ErbB2 levels and improves cell survival. We hypothesize that zinc is capable of modulating redox and endop...

journal_title：Biological chemistry

authors： Bodiga VL,Vemuri PK,Nimmagadda G,Bodiga S

更新日期：2020-10-25 00:00:00

abstract：:Proteomic identification of protease cleavage site specificity (PICS) is a recent proteomic approach for the easy mapping of protease subsite preferences that determines both the prime- and non-prime side specificity concurrently. Here we greatly facilitate user access by providing an automated and simple web-based da...

journal_title：Biological chemistry

authors： Schilling O,auf dem Keller U,Overall CM

更新日期：2011-11-01 00:00:00

abstract：:The surface of spherical, nonporous silica nanoparticles (SiO2-NPs) was modified with gadolinium (Gd) complexes, fluorophores, and cell-penetrating peptides to achieve multifunctionality on a single particle. The Gd surface concentrations were 9-16 μmol/g resulting in nanomaterials with high local longitudinal and tra...

journal_title：Biological chemistry

authors： Joshi R,Feldmann V,Koestner W,Detje C,Gottschalk S,Mayer HA,Sauer MG,Engelmann J

更新日期：2013-01-01 00:00:00