Abstract:
:Proteomic identification of protease cleavage site specificity (PICS) is a recent proteomic approach for the easy mapping of protease subsite preferences that determines both the prime- and non-prime side specificity concurrently. Here we greatly facilitate user access by providing an automated and simple web-based data-analysis resource termed WebPics (http://clipserve.clip.ubc.ca/pics/). We demonstrate the utility of WebPics analysis of PICS data by determining the substrate specificity of factor Xa from P6-P6', an important blood coagulation protease that proteolytically generates thrombin from prothrombin. PICS confirms existing data on non-prime site specificity and refines our knowledge of factor Xa prime-site selectivity.
journal_name
Biol Chemjournal_title
Biological chemistryauthors
Schilling O,auf dem Keller U,Overall CMdoi
10.1515/BC.2011.158subject
Has Abstractpub_date
2011-11-01 00:00:00pages
1031-7issue
11eissn
1431-6730issn
1437-4315journal_volume
392pub_type
杂志文章abstract::γ-Secretase is a membrane-embedded protease complex with presenilin as the catalytic component. Cleavage within the transmembrane domain of the amyloid β-protein precursor (APP) by γ-secretase produces the C-terminus of the amyloid β-peptide (Aβ), a proteolytic product prone to aggregation and strongly linked to Alzhe...
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更新日期:1996-07-01 00:00:00
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