Association of cathepsin E with tumor growth arrest through angiogenesis inhibition and enhanced immune responses.

abstract：:Molecular chaperones of the heat shock protein 70 (Hsp70) family play a crucial role in the presentation of exogenous antigenic peptides by antigen-presenting cells (APCs). In a combined biochemical and immunological approach, we characterize the biochemical interaction of tumor-associated peptides with human Hsp70 an...

journal_title：Biological chemistry

authors： Pandya MJ,Bendz H,Manzenrieder F,Noessner E,Kessler H,Buchner J,Issels RD

更新日期：2009-04-01 00:00:00

abstract：:Among the Ras family, Ran is a unique small G protein. It does not have a lipid modification motif at the C-terminus to bind to the membrane, which is often observed within the Ras family. Ran may therefore interact with a wide range of proteins in various intracellular locations. This means that Ran could play many d...

journal_title：Biological chemistry

authors： Nishimoto T

更新日期：2000-05-01 00:00:00

abstract：:The p53 tumour suppressor gene is frequently mutated in human tumours and different tumour-derived mutations have varying effects on cells. The effect of a novel tumour-derived p53 mutation and two recently described mutations from South African breast cancer patients on the growth rate, colony formation, cell cycle a...

journal_title：Biological chemistry

authors： Donninger H,Binder A,Bohm L,Parker MI

更新日期：2008-01-01 00:00:00

abstract：:The multifunctional helper component proteinase (HC-Pro) of potyviruses contains an autoproteolytic function that, together with the protein 1 (P1) and NIa proteinase, processes the polyprotein into mature proteins. In this study, we analysed the autoproteolytic active domain of zucchini yellow mosaic virus (ZYMV) HC-...

journal_title：Biological chemistry

authors： Boonrod K,Füllgrabe MW,Krczal G,Wassenegger M

更新日期：2011-10-01 00:00:00

abstract：:In a recent study we showed that two proteinases (CMS2MS2 and CMS2MS3) from Carica candamarcensis enhance mammalian cell proliferation. The aim of the present study is the determination of the primary structure of CMS2MS2 and prediction of its three-dimensional structure. The protein contains 214 residues, including t...

journal_title：Biological chemistry

authors： Gomes MT,Bemquerer MP,Lopes MT,Richardson M,Júnior SO,Salas CE

更新日期：2007-08-01 00:00:00

abstract：:A high-resolution crystallographic structure determination of a protein-ligand complex is generally accepted as the 'gold standard' for structure-based drug design, yet the relationship between structure and affinity is neither obvious nor straightforward. Here we analyze the interactions of a series of serine protein...

journal_title：Biological chemistry

authors： Tziridis A,Rauh D,Neumann P,Kolenko P,Menzel A,Bräuer U,Ursel C,Steinmetzer P,Stürzebecher J,Schweinitz A,Steinmetzer T,Stubbs MT

更新日期：2014-07-01 00:00:00

abstract：:Type I allergy, a hypersensitivity disease affecting almost 20% of the population worldwide, is based on the IgE recognition of otherwise harmless antigens (i.e., allergens). Allergen-induced crosslink of effector cell-bound IgE antibodies leads to the release of biological mediators and thus to immediate disease symp...

journal_title：Biological chemistry

authors： Valenta R,Vrtala S,Focke-Tejkl M,Bugajska-Schretter,Ball T,Twardosz A,Spitzauer S,Grönlund H,Kraft D

更新日期：1999-07-01 00:00:00

abstract：:There are enzymatic and non-enzymatic mechanisms that generate NO* from nitrite in blood, stomach, saliva, urine and skin. In blood vessels, nitrite-derived NO* can provide protection via compensatory vasodilation during hypoxia, and in various body fluids it may have antibacterial activity. In the skin, nitrite-deriv...

journal_title：Biological chemistry

authors： Suschek CV,Schewe T,Sies H,Kröncke KD

更新日期：2006-05-01 00:00:00

abstract：:The physiological roles of the intracellular iron and redox regulatory systems are intimately linked. Iron is an essential trace element for most organisms, yet elevated cellular iron levels are a potent generator and amplifier of reactive oxygen species and redox stress. Proteins binding iron or iron-sulfur (Fe/S) cl...

journal_title：Biological chemistry

authors： Mühlenhoff U,Braymer JJ,Christ S,Rietzschel N,Uzarska MA,Weiler BD,Lill R

更新日期：2020-11-26 00:00:00

abstract：:Mutations of the p53 gene may alter the specific regulatory domains of the protein. We examined the conserved domains III, IV and V by SSCP using PCR primers covering exons 5, 6, 7 and 8 from hairy cell leukemia (HCL), polyps, colorectal and gastric carcinomas. A low rate of p53 mutations was detected in HCL and polyp...

journal_title：Biological chemistry

authors： Marcsek ZL,König EA

更新日期：1998-04-01 00:00:00

abstract：:Membrane dynamics are essential for numerous cellular processes in eukaryotic and prokaryotic cells. In eukaryotic cells, membrane fusion and fission are often catalyzed by large GTPases of the dynamin protein family. These proteins couple GTP hydrolysis to membrane deformation, which eventually leads to fusion or fis...

journal_title：Biological chemistry

authors： Bramkamp M

更新日期：2012-11-01 00:00:00

abstract：:Synthetic biologists aim at engineering controllable biological parts such as DNA, RNA and proteins in order to steer biological activities using external inputs. Proteins can be controlled in several ways, for instance by regulating the expression of their encoding genes with small molecules or light. However, post-t...

journal_title：Biological chemistry

authors： Di Ventura B,Mootz HD

更新日期：2019-03-26 00:00:00

abstract：:A central point of regulation in the Wnt/beta-catenin signalling pathway is the formation of the beta-catenin destruction complex. Axin1, an essential negative regulator of Wnt signalling, serves as a scaffold within this complex and is critical for rapid turnover of beta-catenin. To examine the mechanism by which Wnt...

journal_title：Biological chemistry

authors： Sue Ng S,Mahmoudi T,Li VS,Hatzis P,Boersema PJ,Mohammed S,Heck AJ,Clevers H

更新日期：2010-02-01 00:00:00

abstract：:Host T cell activation, a key step in obtaining adaptive immunity against pathogens, is initiated by the binding of the T cell receptor to a foreign antigenic peptide presented by the major histocompatibility complex on the surface of an antigen-presenting cell and, consequently, formation of an immunological synapse....

journal_title：Biological chemistry

authors： Beyersdorf N,Müller N

更新日期：2015-06-01 00:00:00

abstract：:RNAs are key players in life as they connect the genetic code (DNA) with all cellular processes dominated by proteins. They contain a variety of chemical modifications and many RNAs fold into complex structures. Here, we review recent progress in the analysis of RNA modification and structure on the basis of stable is...

journal_title：Biological chemistry

authors： Asadi-Atoi P,Barraud P,Tisne C,Kellner S

更新日期：2019-06-26 00:00:00

abstract：:Molecular chaperones are well known as facilitators of protein folding and assembly. However, in recent years multiple chaperone-assisted degradation pathways have also emerged, including CAP (chaperone-assisted proteasomal degradation), CASA (chaperone-assisted selective autophagy), and CMA (chaperone-mediated autoph...

journal_title：Biological chemistry

authors： Kettern N,Dreiseidler M,Tawo R,Höhfeld J

更新日期：2010-05-01 00:00:00

abstract：:Vascular injury triggers endothelial exocytosis of granules, releasing pro-inflammatory and pro-thrombotic mediators into the blood. Nitric oxide (NO) and reactive oxygen species (ROS) limit vascular inflammation and thrombosis by inhibiting endothelial exocytosis. NO decreases exocytosis by regulating the activity of...

journal_title：Biological chemistry

authors： Lowenstein CJ,Tsuda H

更新日期：2006-10-01 00:00:00

abstract：:The O-acetylation of sialic acids turns out to be one of the most important modifications that influence the diverse biological and pathophysiological properties of glycoconjugates in animals and microorganisms. To understand the functions of this esterification, knowledge of the properties, structures and regulation ...

journal_title：Biological chemistry

authors： Iwersen M,Dora H,Kohla G,Gasa S,Schauer R

更新日期：2003-07-01 00:00:00

abstract：:The amyloid precursor protein (APP) and its processing are widely believed to be central for the etiology of Alzheimer's disease (AD) and appear essential for neuronal development and cell homeostasis in mammals. Many studies show the proteolysis of APP by the proteases α-, β- and γ-secretase, functional aspects of th...

journal_title：Biological chemistry

authors： Coburger I,Hoefgen S,Than ME

更新日期：2014-05-01 00:00:00

abstract：:Mitochondria contain elaborate machineries for the import of precursor proteins from the cytosol. The translocase of the outer mitochondrial membrane (TOM) performs the initial import of precursor proteins and transfers the precursors to downstream translocases, including the presequence translocase and the carrier tr...

journal_title：Biological chemistry

authors： Straub SP,Stiller SB,Wiedemann N,Pfanner N

更新日期：2016-11-01 00:00:00

abstract：:In mammals, betaine of the mitochondrial matrix is used in the cytosol by betaine-homocysteine S-methyltransferase for methionine synthesis. The resulting dimethylglycine is shuttled back into the mitochondrial matrix for further degradation. Nanospray tandem mass spectrometry and N-terminal amino acid sequencing of m...

journal_title：Biological chemistry

authors： Sandu C,Nick P,Hess D,Schiltz E,Garrow TA,Brandsch R

更新日期：2000-07-01 00:00:00

abstract：:Trypsin and chymotrypsin have similar tertiary structures, although very different substrate specificities. Trypsin hydrolyzes peptides at Lys/Arg residues while chymotrypsin recognizes large hydrophobic residues. Recent work has shown that trypsin is not converted into a protease with chymotrypsin-like activity when ...

journal_title：Biological chemistry

authors： Hedstrom L

更新日期：1996-07-01 00:00:00

abstract：:G-protein coupled receptor 119 (GPR119) is emerging as a potential target for the treatment of type 2 diabetes with beneficial effects on glucose homeostasis. This study assessed the insulin-secreting properties of various GPR119 agonists and the distribution of GPR119 in pancreatic islets. Endogenous ligands [oleoyle...

journal_title：Biological chemistry

authors： Moran BM,Abdel-Wahab YH,Flatt PR,McKillop AM

更新日期：2014-04-01 00:00:00

abstract：:The gene product of the yeast VPS1 gene is a member of a family of high-molecular-weight GTP-binding proteins that are involved in diverse cellular processes. The Vps1 protein (Vps1p) was shown to perform an essential function in the yeast secretory pathway. Here, we report the isolation and characterization of a muta...

journal_title：Biological chemistry

authors： Finken-Eigen M,Müller S,Köhrer K

更新日期：1997-10-01 00:00:00

abstract：:The formation of N-glycosidic linkages of glycoproteins involves the ordered assembly of the common Glc3Man9GlcNAc2 core-oligosaccharide on the lipid carrier dolichyl pyrophosphate. Whereas early mannosylation steps occur on the cytoplasmic side of the endoplasmic reticulum with GDP-Man as donor, the final reactions f...

journal_title：Biological chemistry

authors： Sharma CB,Knauer R,Lehle L

更新日期：2001-02-01 00:00:00

abstract：:Fanconi anemia is a fatal, hereditary chromosome instability syndrome of early childhood with progressive pancytopenia and cancer-proneness. Hypersensitivity to alkylating agents points to DNA repair inefficiency. Excess reactive oxygen intermediates and hypersensitivity to oxygen, all features of Fanconi anemia cells...

journal_title：Biological chemistry

authors： Kontou M,Hirsch-Kauffmann M,Schweiger M

更新日期：2008-10-01 00:00:00

abstract：:A kinetic study was performed of a model for an autocatalytic zymogen activation process involving both intra- and intermolecular routes, to which a chromogenic reaction in which the active enzyme acts upon one of its substrates was coupled to continuously monitor the reaction. Kinetic equations describing the evoluti...

journal_title：Biological chemistry

authors： Fuentes ME,Varón R,García-Moreno M,Valero E

更新日期：2005-07-01 00:00:00

abstract：:Small RNAs such as microRNAs (miRNAs), short interfering RNAs (siRNAs) or Piwi-interacting RNAs (piRNAs) are important regulators of gene expression in various organisms. Small RNAs bind to a member of the Argonaute protein family and are incorporated into larger structures that mediate diverse gene silencing events. ...

journal_title：Biological chemistry

authors： Dueck A,Meister G

更新日期：2014-06-01 00:00:00

abstract：:Bauhinia ungulata factor Xa inhibitor (BuXI) inactivates factor Xa and LOPAP, a prothrombin activator proteinase isolated from the venom of Lonomia obliqua caterpillar bristles. The reactive site of the enzyme-inhibitor interaction was explored to design specific substrates for both enzymes. Methionine is crucial for ...

journal_title：Biological chemistry

authors： Andrade SA,Santomauro-Vaz EM,Lopes AR,Chudzinski-Tavassi AM,Juliano MA,Terra WR,Sampaio MU,Sampaio CA,Oliva ML

更新日期：2003-03-01 00:00:00

abstract：:The periplasmic ligand-binding protein ChoX is part of the ABC transport system ChoVWX that imports choline as a nutrient into the soil bacterium Sinorhizobium meliloti. We have recently reported the crystal structures of ChoX in complex with its ligands choline and acetylcholine and the structure of a fully closed bu...

journal_title：Biological chemistry

authors： Oswald C,Smits SH,Höing M,Bremer E,Schmitt L

更新日期：2009-11-01 00:00:00