Abstract：
:The p53 tumour suppressor gene is frequently mutated in human tumours and different tumour-derived mutations have varying effects on cells. The effect of a novel tumour-derived p53 mutation and two recently described mutations from South African breast cancer patients on the growth rate, colony formation, cell cycle arrest after irradiation and response to chemotherapeutic drugs was investigated. None of the p53 mutations had any significant effect on the inherent growth rate of the cells; however, contact inhibition of growth in two of the mutants was lost. These same two mutants formed colonies in soft agar, whereas the third mutant did not. All three of the mutants failed to show a G(1) cell cycle arrest after exposure to 7 Gy of [(60)Co] radiation, albeit to different degrees. Cells expressing the p53 mutants were either more sensitive to cisplatin and melphalan or more resistant than the untransfected cells, depending on the mutation. However, there was no difference in response to daunorubicin treatment. These results demonstrate that different p53 mutations exert varying biological effects on normal cells, with some altering checkpoint activation more effectively than others. The data also suggest that the nature of the p53 mutation influences the sensitivity to cytostatic drugs.
journal_name
Biol Chemjournal_title
Biological chemistryauthors
Donninger H,Binder A,Bohm L,Parker MIdoi
10.1515/BC.2008.010subject
Has Abstractpub_date
2008-01-01 00:00:00pages
57-67issue
1eissn
1431-6730issn
1437-4315journal_volume
389pub_type
杂志文章abstract：:Many aspects of the RNA maturation leave traces in RNA sequencing data in the form of deviations from the reference genomic DNA. This includes, in particular, genomically non-encoded nucleotides and chemical modifications. The latter leave their signatures in the form of mismatches and conspicuous patterns of sequenci...
journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/BC.2011.043
更新日期：2011-04-01 00:00:00
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pub_type： 杂志文章
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journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/BC.2000.137
更新日期：2000-11-01 00:00:00
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pub_type： 杂志文章
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更新日期：2008-09-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2009-02-01 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：1999-09-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2003-06-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2010-09-01 00:00:00
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pub_type： 杂志文章
doi：10.1515/bchm3.1996.377.10.625
更新日期：1996-10-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2009-05-01 00:00:00
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journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/bchm.1998.379.3.335
更新日期：1998-03-01 00:00:00
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journal_title：Biological chemistry
pub_type： 杂志文章,评审
doi：10.1515/bc.2008.051
更新日期：2008-05-01 00:00:00
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pub_type： 杂志文章
doi：10.1515/BC.2002.016
更新日期：2002-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1515/BC.2007.053
更新日期：2007-05-01 00:00:00
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journal_title：Biological chemistry
pub_type： 杂志文章
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更新日期：2000-04-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2001-01-01 00:00:00
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更新日期：2019-03-26 00:00:00
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journal_title：Biological chemistry
pub_type： 杂志文章,评审
doi：
更新日期：1996-07-01 00:00:00
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更新日期：2006-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：2001-02-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2003-05-01 00:00:00
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pub_type： 杂志文章
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更新日期：2010-10-01 00:00:00
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pub_type： 杂志文章
doi：10.1515/BC.2001.016
更新日期：2001-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：2002-02-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2019-09-25 00:00:00
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pub_type： 杂志文章
doi：10.1515/hsz-2017-0208
更新日期：2018-02-23 00:00:00
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journal_title：Biological chemistry
pub_type： 杂志文章
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更新日期：2001-11-01 00:00:00
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journal_title：Biological chemistry
pub_type： 杂志文章
doi：10.1515/hsz-2017-0241
更新日期：2018-04-25 00:00:00
