A novel design of HA-coated nanoparticles co-encapsulating plasmid METase and 5-Fu shows enhanced application in targeting gastric cancer stem cells.

abstract：:We have previously described Kunitz-type serine proteinase inhibitors purified from Bauhinia seeds. Human plasma kallikrein shows different susceptibility to those inhibitors. In this communication, we describe the interaction of human plasma kallikrein with fluorogenic and non-fluorogenic peptides based on the Bauhin...

journal_title：Biological chemistry

authors： Oliva ML,Santomauro-Vaz EM,Andrade SA,Juliano MA,Pott VJ,Sampaio MU,Sampaio CA

更新日期：2001-01-01 00:00:00

abstract：:The transcription factor E2F1 plays a decisive role in the G1/S and G2/M checkpoint transitions of proliferating cells. Because cells are arrested at these checkpoints after heat shock it was of interest to test heat shock effects on E2F1 activity. In human A549 cells, heat shock (44 degrees C, 30 min) caused an immed...

journal_title：Biological chemistry

authors： Gerullis D,Rensing L,Beyersmann D

更新日期：2003-01-01 00:00:00

abstract：:Disruption of the plasma membrane poses deadly threat to eukaryotic cells and survival requires a rapid membrane repair system. Recent evidence reveal various plasma membrane repair mechanisms, which are required for cells to cope with membrane lesions including membrane fusion and replacement strategies, remodeling o...

journal_title：Biological chemistry

authors： Boye TL,Nylandsted J

更新日期：2016-10-01 00:00:00

abstract：:Eremophilanes are sesquiterpenes with a rearranged carbon skeleton formed both by plants and fungi, however, almost no plant eremophilanes are found in fungi. These eremophilanes possess mainly phytotoxic, antimicrobial, anticancer and immunomodulatory properties and in this review fungal eremophilanes with bioactivit...

journal_title：Biological chemistry

authors： Yuyama KT,Fortkamp D,Abraham WR

更新日期：2017-12-20 00:00:00

abstract：:The immunoproteasome subunit low molecular weight protein 2 (LMP2) codon 60 polymorphism has been associated with autoimmune diseases. It has also been demonstrated to influence susceptibility to TNF-alpha-induced apoptosis in blood cells and proteasome activity in aged human brain. In the present study, an in silico ...

journal_title：Biological chemistry

authors： Mishto M,Santoro A,Bellavista E,Sessions R,Textoris-Taube K,Dal Piaz F,Carrard G,Forti K,Salvioli S,Friguet B,Kloetzel PM,Rivett AJ,Franceschi C

更新日期：2006-04-01 00:00:00

abstract：:Calpain is a calcium-activated non lysosomal neutral thiol protease (EC 3.4.22.17) present in a wide variety of eukaryotic cells. Calpain is usually present as an inactive form and is activated by calcium ions and phospholipids. The ability of calpain to alter, by limited proteolysis, the activity or function of numer...

journal_title：Biological chemistry

authors： Yajima Y,Kawashima S

更新日期：2002-05-01 00:00:00

abstract：:Biofilms are a ubiquitous mode of microbial life and display an increased tolerance to different stresses. Inside biofilms, cells may experience both externally applied stresses and internal stresses that emerge as a result of growth in spatially structured communities. In this review, we discuss the spatial scales of...

journal_title：Biological chemistry

authors： Rode DKH,Singh PK,Drescher K

更新日期：2020-11-26 00:00:00

abstract：:An inert polysaccharide scaffold identified as a 5-15% component of prion rods (PrP 27-30) is unambiguously distinguishable from the N-glycosyl groups and the GPI anchor of PrP, and consists predominantly of 1,4-linked glucose with some branching via 1,4,6-linked glucose. We show that this polysaccharide scaffold is a...

journal_title：Biological chemistry

authors： Dumpitak C,Beekes M,Weinmann N,Metzger S,Winklhofer KF,Tatzelt J,Riesner D

更新日期：2005-11-01 00:00:00

abstract：:The enzymatic properties of glycosylphosphatidylinositol-specific phospholipase D (EC 3.1.4.50) were characterized using a 6,000-fold purified enzyme. This was obtained in 100 microg amounts from human serum with a recovery of 35%. Pure alkaline phosphatase containing one anchor moiety per molecule was used as substra...

journal_title：Biological chemistry

authors： Rhode H,Schulze M,Cumme GA,Göhlert A,Blume E,Bublitz R,Schilling K,Horn A

更新日期：2000-05-01 00:00:00

abstract：:We present a method that allows preparing long DNA containing defined mismatches without the use of gel electrophoretic or chromatographic purification steps. The preparation starts with the synthesis of two PCR products, which are identical except for those positions that will later form the mismatches. One of the PC...

journal_title：Biological chemistry

authors： Thomas E,Pingoud A,Friedhoff P

更新日期：2002-09-01 00:00:00

abstract：:The neurobiological mechanisms of obesity-induced peripheral neuropathy are poorly understood. We evaluated the role of Sigma-1 receptor (Sig-1R) and NMDA receptor (NMDARs) in the spinal cord in peripheral neuropathy using an animal model of high fat diet-induced diabetes. We examined the expression of Sig-1R and NMDA...

journal_title：Biological chemistry

authors： Song T,Zhao J,Ma X,Zhang Z,Jiang B,Yang Y

更新日期：2017-09-26 00:00:00

abstract：:There is increasing evidence that apoptosis and necrosis represent only two of several possible ways for cells to die. These two types of demise can occur simultaneously in tissues or cell cultures exposed to the same stimulus, and often local metabolic conditions and the intensity of the same initial insult decide th...

journal_title：Biological chemistry

authors： Nicotera P,Leist M,Single B,Volbracht C

更新日期：1999-09-01 00:00:00

abstract：:Transforming growth factor-β (TGFβ) signaling acts as suppressor and inducer of tumor progression during the early and late stages of cancer, respectively. Some miRNAs have shown a regulatory effect on TGFβ signaling and here, we have used a combination of bioinformatics and experimental tools to show that hsa-miR-559...

journal_title：Biological chemistry

authors： Abedini Bakhshmand E,Soltani BM

更新日期：2019-04-24 00:00:00

abstract：:Full-length Hsp70 protein (Hsp70) and the C-terminal domain of Hsp70 (Hsp70C) both stimulate the cytolytic activity of naive natural killer (NK) cells against Hsp70-positive tumor target cells. Here, we describe the characterization of Hsp70-NK cell interaction with binding studies using the human NK cell line YT. Bin...

journal_title：Biological chemistry

authors： Gross C,Hansch D,Gastpar R,Multhoff G

更新日期：2003-02-01 00:00:00

abstract：:The HIRIP3 protein had been identified from its interaction with the HIRA histone chaperone. Experiments using anti-peptide antisera indicated that this 556-aa protein is nuclear throughout the cell cycle and excluded from condensed chromatin during mitosis. Based on its electrophoretic migration and sensitivity to ph...

journal_title：Biological chemistry

authors： Assrir N,Filhol O,Galisson F,Lipinski M

更新日期：2007-04-01 00:00:00

abstract：:Gaucher disease (GD) is a lysosomal storage disorder, caused by an impaired function of β-glucocerebrosidase, which results in accumulation of glucocerebroside in cells, and altered membrane ordering. Using electron paramagnetic resonance spin labeling, a statistically significant difference in the order parameter bet...

journal_title：Biological chemistry

authors： Pavićević A,Lakočević M,Popović M,Popović-Bijelić A,Daković M,Mojović M

更新日期：2018-04-25 00:00:00

abstract：:The heterodimer consisting of ecdysteroid receptor (EcR) and ultraspiracle (USP), both of which are members of the nuclear receptor superfamily, is considered to be the functional ecdysteroid receptor. Here we analyzed the subcellular distribution of EcR and USP fused to fluorescent proteins. The experiments were carr...

journal_title：Biological chemistry

authors： Nieva C,Gwóźdź T,Dutko-Gwóźdź J,Wiedenmann J,Spindler-Barth M,Wieczorek E,Dobrucki J,Duś D,Henrich V,Ozyhar A,Spindler KD

更新日期：2005-05-01 00:00:00

abstract：:Airway smooth muscle (ASM) controls airway narrowing and plays a pivotal role in the pathogenesis of asthma. MicroRNAs are small yet powerful gene tuners that regulate diverse cellular processes. Recent studies have demonstrated the versatile role of microRNAs in regulating multiple ASM phenotypes that are critically ...

journal_title：Biological chemistry

authors： Sun M,Lu Q

更新日期：2016-06-01 00:00:00

abstract：:The fundamental cellular role and molecular interactions of annexins in vesicle trafficking and membrane remodeling remain to be further clarified in order to better understand and exploit their contributions to health and disease. We focused on distinctive features of atypical annexins from all domains of life using ...

journal_title：Biological chemistry

authors： Fernandez MP,Garcia M,Martin-Almedina S,Morgan RO

更新日期：2017-06-27 00:00:00

abstract：:Matrix metalloproteases from the cell surface cleave an 80 kDa E-cadherin fragment (sE-CAD) that induces invasion of cancer cells into collagen type I and inhibits cellular aggregation. Conditioned media from MDCKts.srcCl2 cells at 40 degrees C and 35 degrees C, PCm.src5 and COLO-16 cells at 37 degrees C contained spo...

journal_title：Biological chemistry

authors： Ryniers F,Stove C,Goethals M,Brackenier L,Noë V,Bracke M,Vandekerckhove J,Mareel M,Bruyneel E

更新日期：2002-01-01 00:00:00

abstract：:In a recent study we showed that two proteinases (CMS2MS2 and CMS2MS3) from Carica candamarcensis enhance mammalian cell proliferation. The aim of the present study is the determination of the primary structure of CMS2MS2 and prediction of its three-dimensional structure. The protein contains 214 residues, including t...

journal_title：Biological chemistry

authors： Gomes MT,Bemquerer MP,Lopes MT,Richardson M,Júnior SO,Salas CE

更新日期：2007-08-01 00:00:00

abstract：:MHC (major histocompatibility complex) class I molecules bind intracellular virus-derived peptides in the endoplasmic reticulum (ER) and present them at the cell surface to cytotoxic T lymphocytes. Peptide-free class I molecules at the cell surface, however, could lead to aberrant T cell killing. Therefore, cells ensu...

journal_title：Biological chemistry

authors： Wright CA,Kozik P,Zacharias M,Springer S

更新日期：2004-09-01 00:00:00

abstract：:The saline extract of Bauhinia bauhinioides dry seeds was shown to inhibit cruzipain, a cysteine proteinase from Trypanosoma cruzi. The inhibitory activity was assigned to a protein with 164 amino acid residues and molecular mass of 18 034 Da that was purified by chromatography on DEAE-Sephadex, trypsin-Sepharose (rem...

journal_title：Biological chemistry

authors： de Oliveira C,Santana LA,Carmona AK,Cezari MH,Sampaio MU,Sampaio CA,Oliva ML

更新日期：2001-05-01 00:00:00

abstract：:Understanding the molecular mechanisms behind extreme temperature stability is of relevance for the protein folding problem and for designing proteins for industrial and medical applications. A powerful approach for understanding the structural basis of thermostability is the comparison of high resolution structures o...

journal_title：Biological chemistry

authors： Macedo-Ribeiro S,Darimont B,Sterner R

更新日期：1997-03-01 00:00:00

abstract：:Cancer cells are relatively resistant to endoplasmic reticulum (ER) stress-induced apoptosis. However, the underlying mechanisms remain largely unclear. We observed that the microRNAs miR-221/222 are associated with apoptosis regulation under ER stress in human hepatocellular carcinoma (HCC) cells. Induction of ER str...

journal_title：Biological chemistry

authors： Dai R,Li J,Liu Y,Yan D,Chen S,Duan C,Liu X,He T,Li H

更新日期：2010-07-01 00:00:00

abstract：:Kallikrein 6 (KLK6) is a trypsin-like serine peptidase whose relevance in various types of cancers is currently being explored. Previous studies have shown that KLK6 mRNA is upregulated in colon and gastric cancers; however, the regulatory mechanisms and phenotypic consequences of this upregulation are largely unknown...

journal_title：Biological chemistry

authors： Henkhaus RS,Gerner EW,Ignatenko NA

更新日期：2008-06-01 00:00:00

abstract：:Acid ceramidase (N-acylsphingosine deacylase, EC 3.5.1.23; AC) is the lipid hydrolase responsible for the degradation of ceramide into sphingosine and free fatty acids within lysosomes. The enzymatic activity was first identified over four decades ago and is deficient in two rare inherited disorders, Farber lipogranul...

journal_title：Biological chemistry

authors： Frohbergh M,He X,Schuchman EH

更新日期：2015-06-01 00:00:00

abstract：:The mitochondrial DNA (mtDNA) encompasses two classes of functionally important sequence variants: recent pathogenic mutations and ancient adaptive polymorphisms. To rapidly and cheaply evaluate both classes of single nucleotide variants (SNVs), we have developed an integrated system in which mtDNA SNVs are analyzed b...

journal_title：Biological chemistry

authors： Poole JC,Procaccio V,Brandon MC,Merrick G,Wallace DC

更新日期：2010-10-01 00:00:00

abstract：:The central process for the transfer of the genetic information from the nucleic acid world into the structure of proteins is the ribosomal elongation cycle, where the sequence of codons is translated into the sequence of amino acids. The nascent polypeptide chain is elongated by one amino acid during the reactions of...

journal_title：Biological chemistry

authors： Spahn CM,Nierhaus KH

更新日期：1998-07-01 00:00:00

abstract：:Characterization of protein damage during photosensitization of chlorin e6-treated cells was performed using the green fluorescent protein (GFP). The GFP-chromophore damage caused by singlet oxygen was studied in COS 7 kidney cells and E. coli bacteria following light irradiation. Electron spin resonance (ESR) reveale...

journal_title：Biological chemistry

authors： Greenbaum L,Rothmann C,Lavie R,Malik Z

更新日期：2000-12-01 00:00:00