MicroRNA regulation of airway smooth muscle function.

abstract：:92 kDa and 72 kDa gelatinases, two neutral proteinases exhibiting elastinolytic activity and secreted as zymogens by aortic smooth muscle cells, were shown to bind to insoluble elastin. The active form of each enzyme interacted with substrate more avidly than latent form. Once bound to insoluble elastin, 92 kDa progel...

journal_title：Biological chemistry

authors： Emonard H,Hornebeck W

更新日期：1997-03-01 00:00:00

abstract：:Plasma prekallikrein (PPK) is synthesised in hepatocytes and secreted into the blood, where it participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. Recently we demonstrated by quantitative RT-PCR that the human PPK gene is transcribed not only in the l...

journal_title：Biological chemistry

authors： Fink E,Bhoola KD,Snyman C,Neth P,Figueroa CD

更新日期：2007-09-01 00:00:00

abstract：:A central point of regulation in the Wnt/beta-catenin signalling pathway is the formation of the beta-catenin destruction complex. Axin1, an essential negative regulator of Wnt signalling, serves as a scaffold within this complex and is critical for rapid turnover of beta-catenin. To examine the mechanism by which Wnt...

journal_title：Biological chemistry

authors： Sue Ng S,Mahmoudi T,Li VS,Hatzis P,Boersema PJ,Mohammed S,Heck AJ,Clevers H

更新日期：2010-02-01 00:00:00

abstract：:The periplasmic ligand-binding protein ChoX is part of the ABC transport system ChoVWX that imports choline as a nutrient into the soil bacterium Sinorhizobium meliloti. We have recently reported the crystal structures of ChoX in complex with its ligands choline and acetylcholine and the structure of a fully closed bu...

journal_title：Biological chemistry

authors： Oswald C,Smits SH,Höing M,Bremer E,Schmitt L

更新日期：2009-11-01 00:00:00

abstract：:The influence of the cellular milieu, a complex and crowded solvent, is often neglected when biomolecular structure and function are studied in vitro. To mimic the cellular environment, crowding effects are commonly induced in vitro using artificial crowding agents like Ficoll or dextran. However, it is unclear if suc...

journal_title：Biological chemistry

authors： Gnutt D,Ebbinghaus S

更新日期：2016-01-01 00:00:00

abstract：:Thyroid hormones stimulate gap junctional communication in rat liver WB-F344 epithelial cells, elevating connexin43 mRNA and protein levels. In the present work we analysed connexin43 expression in liver and heart samples from thyroid hormone-treated Wistar rats. Connexin43 mRNA was elevated 2.1-fold in rat liver samp...

journal_title：Biological chemistry

authors： Stock A,Sies H

更新日期：2000-09-01 00:00:00

abstract：:Protein cofactors often are the business ends of proteins, and are either synthesized inside cells or are taken up from the nutrition. A cofactor that strictly needs to be synthesized by cells is the iron-sulfur (Fe/S) cluster. This evolutionary ancient compound performs numerous biochemical functions including electr...

journal_title：Biological chemistry

authors： Lill R

更新日期：2020-05-26 00:00:00

abstract：:The central process for the transfer of the genetic information from the nucleic acid world into the structure of proteins is the ribosomal elongation cycle, where the sequence of codons is translated into the sequence of amino acids. The nascent polypeptide chain is elongated by one amino acid during the reactions of...

journal_title：Biological chemistry

authors： Spahn CM,Nierhaus KH

更新日期：1998-07-01 00:00:00

abstract：:The three-dimensional structure of pterin-4a-carbinolamine dehydratase (PCD) from Pseudomonas aeruginosa has been solved. Based on this we have investigated the roles of putative active center residues through functional replacement by site-directed mutagenesis. Three histidines, His73, His74 and His91, appear to be i...

journal_title：Biological chemistry

authors： Köster S,Stier G,Kubasch N,Curtius HC,Ghisla S

更新日期：1998-12-01 00:00:00

abstract：:Cellular volume regulation is fundamental for numerous physiological processes. The volume-regulated anion channel, VRAC, plays a crucial role in regulatory volume decrease. This channel, which is ubiquitously expressed in vertebrates, has been vastly characterized by electrophysiological means. It opens upon cell swe...

journal_title：Biological chemistry

authors： Stauber T

更新日期：2015-09-01 00:00:00

abstract：:Maintenance of cellular redox control is pivotal for normal cellular functions and cell fate decisions including cell death. Among the key cellular redox systems in mammals, the glutathione peroxidase (GPX) family of proteins is the largest conferring multifaceted functions and affecting virtually all cellular process...

journal_title：Biological chemistry

authors： Buday K,Conrad M

更新日期：2020-10-22 00:00:00

abstract：:Type I interferons (IFNs) produced primarily by plasmacytoid dendritic cells (pDCs) as part of the innate immune response to infectious agents induce the maturation of myeloid DCs and enhance antigen presentation. Type I IFNs also enhance apoptosis of virus-infected cells, stimulate cross priming and enhanced presenta...

journal_title：Biological chemistry

authors： Tovey MG,Lallemand C,Thyphronitis G

更新日期：2008-05-01 00:00:00

abstract：:Five phage displayed peptide libraries were screened for binders to C1q, the recognition subunit of the classical complement pathway. Two rounds of panning resulted in the isolation and characterisation of several different phage displayed C1q-binding peptides from all five libraries. Two groups of the characterised p...

journal_title：Biological chemistry

authors： Lauvrak V,Brekke OH,Ihle O,Lindqvist BH

更新日期：1997-12-01 00:00:00

abstract：:Membrane dynamics are essential for numerous cellular processes in eukaryotic and prokaryotic cells. In eukaryotic cells, membrane fusion and fission are often catalyzed by large GTPases of the dynamin protein family. These proteins couple GTP hydrolysis to membrane deformation, which eventually leads to fusion or fis...

journal_title：Biological chemistry

authors： Bramkamp M

更新日期：2012-11-01 00:00:00

abstract：:Calpain is a calcium-activated non lysosomal neutral thiol protease (EC 3.4.22.17) present in a wide variety of eukaryotic cells. Calpain is usually present as an inactive form and is activated by calcium ions and phospholipids. The ability of calpain to alter, by limited proteolysis, the activity or function of numer...

journal_title：Biological chemistry

authors： Yajima Y,Kawashima S

更新日期：2002-05-01 00:00:00

abstract：:The ligand-activated aryl hydrocarbon receptor (AHR) is known to modulate many genes in a highly cell-specific manner, either directly or indirectly via secondary effects. In contrast, little is known about the effects of AHR deficiency on gene expression balance. We compared the transcriptome of CD4 T cells from AHR-...

journal_title：Biological chemistry

authors： Frericks M,Temchura VV,Majora M,Stutte S,Esser C

更新日期：2006-09-01 00:00:00

abstract：:Xeroderma pigmentosum complementation group D protein (XPD) is an iron-sulfur cluster containing 5'-3' helicase and, in humans, part of the transcription factor TFIIH. TFIIH is involved in nucleotide excision repair as well as in transcription initiation. Recently, three different groups have reported the structures o...

journal_title：Biological chemistry

authors： Wolski SC,Kuper J,Kisker C

更新日期：2010-07-01 00:00:00

abstract：:Interleukin-6 (IL-6) is a 185 amino acid residue helical cytokine with various biological activities (e. g. B cell development, acute phase reaction). We have investigated the role of the 168-185 C-terminal region of IL-6 in the induction of fibrinogen synthesis and expression of junB mRNA using synthetic peptides cor...

journal_title：Biological chemistry

authors： Igaz P,Bösze S,Tóth S,Falus A,Hudecz F

更新日期：2001-04-01 00:00:00

abstract：:Invadolysin is a novel metalloprotease conserved amongst metazoans that is essential for life in Drosophila. We previously showed that invadolysin was essential for the cell cycle and cell migration, linking to metabolism through a role in lipid storage and interaction with mitochondrial proteins. In this study we dem...

journal_title：Biological chemistry

authors： Chang CW,Abhinav K,Di Cara F,Panagakou I,Vass S,Heck MM

更新日期：2017-03-01 00:00:00

abstract：:The mitochondrial DNA (mtDNA) encompasses two classes of functionally important sequence variants: recent pathogenic mutations and ancient adaptive polymorphisms. To rapidly and cheaply evaluate both classes of single nucleotide variants (SNVs), we have developed an integrated system in which mtDNA SNVs are analyzed b...

journal_title：Biological chemistry

authors： Poole JC,Procaccio V,Brandon MC,Merrick G,Wallace DC

更新日期：2010-10-01 00:00:00

abstract：:In order to study the role of N-glycans in the ER-associated degradation of unassembled immunoglobulin light (Ig L) chains, we introduced N-glycan acceptor sites into the variable domain of the murine Ig L chain kappaNS1, which is unfolded in unassembled molecules. We investigated the fate of kappaNS1 glycosylated at ...

journal_title：Biological chemistry

authors： Chillarón J,Adán C,Haas IG

更新日期：2000-12-01 00:00:00

abstract：:Glutaminyl cyclases (QC) catalyze the intramolecular cyclization of N-terminal glutamine residues of peptides and proteins. For a comparison of the substrate specificity of human and papaya QC enzymes, a novel continuous assay was established by adapting an existing discontinuous method. Specificity constants (kcat/Km...

journal_title：Biological chemistry

authors： Schilling S,Manhart S,Hoffmann T,Ludwig HH,Wasternack C,Demuth HU

更新日期：2003-12-01 00:00:00

abstract：:Myocardial zinc dyshomeostasis is associated with caspase-3 activation, ErbB2 degradation and apoptosis during hypoxia/reoxygenation. Zinc pyrithione replenishes intracellular zinc, suppresses caspase-3, augments ErbB2 levels and improves cell survival. We hypothesize that zinc is capable of modulating redox and endop...

journal_title：Biological chemistry

authors： Bodiga VL,Vemuri PK,Nimmagadda G,Bodiga S

更新日期：2020-10-25 00:00:00

abstract：:Rhomboids are ubiquitous intramembrane serine proteases that cleave transmembrane substrates. Their functions include growth factor signaling, mitochondrial homeostasis, and parasite invasion. A recent study revealed that the Escherichia coli rhomboid protease EcGlpG is essential for its extraintestinal pathogenic col...

journal_title：Biological chemistry

authors： Arutyunova E,Jiang Z,Yang J,Kulepa AN,Young HS,Verhelst S,O'Donoghue AJ,Lemieux MJ

更新日期：2018-11-27 00:00:00

abstract：:Pseudomonas aeruginosa and Serratia marcescens can cause refractory keratitis resulting in corneal perforation and blindness. These bacteria produce various kinds of proteases. In addition to pseudomonal elastase (LasB) and alkaline protease, LasA protease and protease IV have recently been found to be more important ...

journal_title：Biological chemistry

authors： Matsumoto K

更新日期：2004-11-01 00:00:00

abstract：:The multifunctional helper component proteinase (HC-Pro) of potyviruses contains an autoproteolytic function that, together with the protein 1 (P1) and NIa proteinase, processes the polyprotein into mature proteins. In this study, we analysed the autoproteolytic active domain of zucchini yellow mosaic virus (ZYMV) HC-...

journal_title：Biological chemistry

authors： Boonrod K,Füllgrabe MW,Krczal G,Wassenegger M

更新日期：2011-10-01 00:00:00

abstract：:The BCL2 family of genes (B-cell CLL/lymphoma 2; Bcl-2) plays a pivotal role in the highly regulated process of apoptosis. We have recently cloned a newly identified member of this family, BCL2L12, which was found to be differentially expressed in many tumors. It is known that topotecan and methotrexate act through in...

journal_title：Biological chemistry

authors： Floros KV,Talieri M,Scorilas A

更新日期：2006-12-01 00:00:00

abstract：:The cloning of a Ca2+(0)-sensing receptor (CaR) from parathyroid and kidney, coupled with the identification of inherited disorders in Ca2+(0)-sensing resulting from inactivating or activating mutations of this CaR, has provided substantial insights into how Ca2+(0) is regulated at a systemic level. The CaR plays a ce...

journal_title：Biological chemistry

authors： Chattopadhyay N,Vassilev PM,Brown EM

更新日期：1997-08-01 00:00:00

abstract：:Studies of extracellular matrix (ECM) biology in the nervous system have mainly focused on laminin, fibronectin and tenascin-R, proteins that are present during nervous system development and normal function. However, during disease, fibrin, which physiologically is not present in the nervous tissue, is detected at ne...

journal_title：Biological chemistry

authors： Akassoglou K,Strickland S

更新日期：2002-01-01 00:00:00

abstract：:In pancreatic acinar cells stimulation of different intracellular pathways leads to different patterns of Ca2+ signaling. Bombesin induces activation of both phosphatidylinositol 4,5-bisphosphate (PIP2)-specific phospholipase C (PLC) and phospholipase D (PLD). The latter leads to generation of diacylglycerol (DAG) in ...

journal_title：Biological chemistry

authors： Schulz I,Krause E,González A,Göbel A,Sternfeld L,Schmid A

更新日期：1999-07-01 00:00:00