Transcriptional signatures of immune cells in aryl hydrocarbon receptor (AHR)-proficient and AHR-deficient mice.

abstract：:Thyroid hormones stimulate gap junctional communication in rat liver WB-F344 epithelial cells, elevating connexin43 mRNA and protein levels. In the present work we analysed connexin43 expression in liver and heart samples from thyroid hormone-treated Wistar rats. Connexin43 mRNA was elevated 2.1-fold in rat liver samp...

journal_title：Biological chemistry

authors： Stock A,Sies H

更新日期：2000-09-01 00:00:00

abstract：:Heparan sulfate proteoglycans are obligatory for receptor binding and mitogenic activity of the basic fibroblast growth factor (bFGF). In the present study the influence of undersulfated heparan sulfate on the expression of basic fibroblast growth factor and coronary smooth muscle cell (cSMC) proliferation was investi...

journal_title：Biological chemistry

authors： Schriever C,Breithardt G,Schmidt A

更新日期：1997-07-01 00:00:00

abstract：:Site-directed spin labeling (SDSL) in combination with electron paramagnetic resonance (EPR) spectroscopy has emerged as an efficient tool to elucidate the structure and the conformational dynamics of proteins under conditions close to the native state. This review article summarizes the basics as well as the recent p...

journal_title：Biological chemistry

authors： Klare JP

更新日期：2013-10-01 00:00:00

abstract：:The Sialyl-Tn antigen (Sialyl alpha-Ser/Thr) is expressed as a cancer-associated antigen on the surface of cancer cells. Its presence is associated with a poor prognosis in patients with colorectal and other cancers. We previously reported that Sialyl-Tn expression in LSC human colon cancer cells could be explained by...

journal_title：Biological chemistry

authors： Brockhausen I,Yang J,Lehotay M,Ogata S,Itzkowitz S

更新日期：2001-02-01 00:00:00

abstract：:A kinetic study was performed of a model for an autocatalytic zymogen activation process involving both intra- and intermolecular routes, to which a chromogenic reaction in which the active enzyme acts upon one of its substrates was coupled to continuously monitor the reaction. Kinetic equations describing the evoluti...

journal_title：Biological chemistry

authors： Fuentes ME,Varón R,García-Moreno M,Valero E

更新日期：2005-07-01 00:00:00

abstract：:Two new fluorescent labels are presented that are optimized for excitation with He/Ne laser and red diode lasers. Application in FCS and labeling of proteins and oligomers are demonstrated. A strong rise of quantum yield and emission life time upon binding to biomolecules are characteristic features of the dyes. ...

journal_title：Biological chemistry

authors： Czerney P,Lehmann F,Wenzel M,Buschmann V,Dietrich A,Mohr GJ

更新日期：2001-03-01 00:00:00

abstract：:Stromal cell-derived factor-1alpha (SDF-1alpha) is a strong migratory stimulant for hematopoietic stem and progenitor cells (HSPCs). The hematopoietic cytokines thrombopoietin (TPO), Flt3-ligand (FL), stem cell factor (SCF) and interleukin 11 (IL-11) are able to stimulate amplification of primitive murine hematopoieti...

journal_title：Biological chemistry

authors： Kassmer SH,Niggemann B,Punzel M,Mieck C,Zänker KS,Dittmar T

更新日期：2008-07-01 00:00:00

abstract：:Hypochlorous acid (HOCl) is a bactericidal compound formed by activated neutrophils during inflammation. Overproduction of HOCl causes damage to tissues at the site of neutrophil accumulation. The deleterious effects of excessive HOCl formation can be attenuated using antioxidants. Thiols and thioethers are known to b...

journal_title：Biological chemistry

authors： den Hartog GJ,Haenen GR,Vegt E,van der Vijgh WJ,Bast A

更新日期：2002-03-01 00:00:00

abstract：:Kallikrein 6 (KLK6) is a trypsin-like serine peptidase whose relevance in various types of cancers is currently being explored. Previous studies have shown that KLK6 mRNA is upregulated in colon and gastric cancers; however, the regulatory mechanisms and phenotypic consequences of this upregulation are largely unknown...

journal_title：Biological chemistry

authors： Henkhaus RS,Gerner EW,Ignatenko NA

更新日期：2008-06-01 00:00:00

abstract：:The primary cilium is a sensory organelle that is vital in regulating several signalling pathways. Unlike most organelles cilia are open to the rest of the cell, not enclosed by membranes. The distinct protein composition is crucial to the function of cilia and many signalling proteins and receptors are specifically c...

journal_title：Biological chemistry

authors： Stephen LA,Elmaghloob Y,Ismail S

更新日期：2017-12-20 00:00:00

abstract：:Plasma prekallikrein (PPK) is synthesised in hepatocytes and secreted into the blood, where it participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. Recently we demonstrated by quantitative RT-PCR that the human PPK gene is transcribed not only in the l...

journal_title：Biological chemistry

authors： Fink E,Bhoola KD,Snyman C,Neth P,Figueroa CD

更新日期：2007-09-01 00:00:00

abstract：:The lysosomal lumen contains numerous acidic hydrolases involved in the degradation of carbohydrates, lipids, proteins, and nucleic acids, which are basic cell components that turn over continuously within the cell and/or are ingested from outside of the cell. Deficiency in almost any of these hydrolases causes accumu...

journal_title：Biological chemistry

authors： Golabek AA,Kida E

更新日期：2006-08-01 00:00:00

abstract：:MHC encoded DM heterodimers and classical MHC class II complexes meet in an endosomal/lysosomal compartment where DM heterodimers support peptide loading of MHC class II. Studies on peptide loading of rat class II and on peptide persistence in cells of the dendritic lineage prompted us to establish full length cDNA cl...

journal_title：Biological chemistry

authors： Kurth D,Neumann J,Demleitner K,Hildmann J,Mehlig M,Scheicher C,Wendling U,Sun D,Reske K

更新日期：1997-09-01 00:00:00

abstract：:Molecular chaperones of the heat shock protein 70 (Hsp70) family play a crucial role in the presentation of exogenous antigenic peptides by antigen-presenting cells (APCs). In a combined biochemical and immunological approach, we characterize the biochemical interaction of tumor-associated peptides with human Hsp70 an...

journal_title：Biological chemistry

authors： Pandya MJ,Bendz H,Manzenrieder F,Noessner E,Kessler H,Buchner J,Issels RD

更新日期：2009-04-01 00:00:00

abstract：:Bacterial infections often upregulate the plasma kinin-forming cascade of the host (the 'contact system') which is triggered by adsorption of high molecular mass kininogen (HK), coagulation factor XII (FXII) and prekallikrein (pHPK) on the host or pathogen cell surfaces. A possible activation of the contact system upo...

journal_title：Biological chemistry

authors： Karkowska-Kuleta J,Kozik A,Rapala-Kozik M

更新日期：2010-01-01 00:00:00

abstract：:The influence of the cellular milieu, a complex and crowded solvent, is often neglected when biomolecular structure and function are studied in vitro. To mimic the cellular environment, crowding effects are commonly induced in vitro using artificial crowding agents like Ficoll or dextran. However, it is unclear if suc...

journal_title：Biological chemistry

authors： Gnutt D,Ebbinghaus S

更新日期：2016-01-01 00:00:00

abstract：:Recent reports presented contradictory results regarding the catabolism of mature atrial (ANP) and brain (BNP) natriuretic peptides in circulation. Especially the role of neutral endopeptidase (NEP) in BNP degradation was conversely discussed. Our present in vitro-studies characterize the NEP-dependent metabolism of A...

journal_title：Biological chemistry

authors： Walther T,Stepan H,Pankow K,Becker M,Schultheiss HP,Siems WE

更新日期：2004-02-01 00:00:00

abstract：:The physiological roles of the intracellular iron and redox regulatory systems are intimately linked. Iron is an essential trace element for most organisms, yet elevated cellular iron levels are a potent generator and amplifier of reactive oxygen species and redox stress. Proteins binding iron or iron-sulfur (Fe/S) cl...

journal_title：Biological chemistry

authors： Mühlenhoff U,Braymer JJ,Christ S,Rietzschel N,Uzarska MA,Weiler BD,Lill R

更新日期：2020-11-26 00:00:00

abstract：:Protein folding is one of the fundamental processes in life and therefore needs to be tightly regulated. Many cellular quality control systems are in place to ensure that proteostasis is optimally adjusted for a changing environment, facilitating protein folding, translocation and degradation. These systems include th...

journal_title：Biological chemistry

authors： Olzscha H

更新日期：2019-06-26 00:00:00

abstract：:Only one transcript for KLK10 was identified by RT-PCR in lung tissue, whereas KLK11 expressed at least four alternative transcripts. Quantitative analysis of KLK10 and KLK11 expression levels was assessed by real-time PCR, in a cohort of 47 patients with non-small-cell lung cancer (NSCLC). Expression levels of these ...

journal_title：Biological chemistry

authors： Planque C,Aïnciburu M,Heuzé-Vourc'h N,Régina S,de Monte M,Courty Y

更新日期：2006-06-01 00:00:00

abstract：:The infectivity and pathogenicity of influenza virus are primarily determined by host cellular trypsin-type processing proteases which cleave the viral membrane fusion glycoprotein hemagglutinin (HA). Therefore the distribution of the processing protease is a major determinant of the infectious organ tropism. The comm...

journal_title：Biological chemistry

authors： Yao D,Chen Y,Kuwajima M,Shiota M,Kido H

更新日期：2004-06-01 00:00:00

abstract：:Coxsackievirus B3 (CVB-3) is a plus-strand RNA virus that is believed to be the most common causal agent of viral myocarditis. Since no specific treatment for CVB-3 infections is available to date, we and others have recently started to develop RNA interference (RNAi) approaches to prevent virus propagation. Here we d...

journal_title：Biological chemistry

authors： Werk D,Schubert S,Lindig V,Grunert HP,Zeichhardt H,Erdmann VA,Kurreck J

更新日期：2005-09-01 00:00:00

abstract：:The N-terminal propeptide domains of several cathepsin L-like cysteine proteases have been shown to possess potent inhibitory activity. Here we report the first kinetic characterisation of the inhibition properties of the cathepsin V propeptide (CatV PP). Using a facile recombinant approach we demonstrate expression, ...

journal_title：Biological chemistry

authors： Burden RE,Snoddy P,Jefferies CA,Walker B,Scott CJ

更新日期：2007-05-01 00:00:00

abstract：:Human kallikrein-related peptidases (KLKs), including the well-known prostate cancer biomarker, prostate-specific antigen (PSA-KLK3), along with 14 other serine proteinase KLK family members are now known to regulate cells by cleaving and activating members of the G-protein-coupled proteinase-activated receptor (PAR) ...

journal_title：Biological chemistry

authors： Hollenberg MD

更新日期：2014-09-01 00:00:00

abstract：:92 kDa and 72 kDa gelatinases, two neutral proteinases exhibiting elastinolytic activity and secreted as zymogens by aortic smooth muscle cells, were shown to bind to insoluble elastin. The active form of each enzyme interacted with substrate more avidly than latent form. Once bound to insoluble elastin, 92 kDa progel...

journal_title：Biological chemistry

authors： Emonard H,Hornebeck W

更新日期：1997-03-01 00:00:00

abstract：:SlyD is a bacterial two-domain protein that functions as a molecular chaperone, a prolyl cis/trans isomerase, and a nickel-binding protein. This review summarizes recent findings about the molecular enzyme mechanism of SlyD. The chaperone function located in one domain of SlyD is involved in twin-arginine translocatio...

journal_title：Biological chemistry

authors： Kovermann M,Schmid FX,Balbach J

更新日期：2013-08-01 00:00:00

abstract：:The influence of the hyaluronan-binding protease (PHBSP), a plasma enzyme with FVII- and pro-urokinase-activating potency, on components of the contact phase (kallikrein/kinin) system was investigated. No activation or cleavage of the proenzymes involved in the contact phase system was observed. The pro-cofactor high ...

journal_title：Biological chemistry

authors： Etscheid M,Beer N,Fink E,Seitz R,Johannes D

更新日期：2002-10-01 00:00:00

abstract：:Interleukin-1 signaling is initiated by recruitment of adapter proteins and kinases to the type I interleukin-1 receptor (IL-1RI). It is modulated by accompanying redox processes at various levels, such as (auto-) phosphorylation of the IL-1RI-associated kinase IRAK, the phosphorylation of IkappaB and translocation an...

journal_title：Biological chemistry

authors： Böl GF,Jurrmann N,Brigelius-Flohé R

更新日期：2003-04-01 00:00:00

abstract：:Oxidative stress is a widespread phenomenon in the pathology of neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, and amyotrophic lateral sclerosis. Neuronal cell death due to oxidative stress may causally contribute to the pathogeneses of these diseases. Therefore, neuroprotective antioxida...

journal_title：Biological chemistry

authors： Moosmann B,Skutella T,Beyer K,Behl C

更新日期：2001-11-01 00:00:00

abstract：:Bromelain isoinhibitors from pineapple stem (BIs) are unique double-chain inhibitors and inhibit the cysteine proteinase bromelain competitively. The three-dimensional structure was shown to be composed of two distinct domains, each of which is formed by a three-stranded anti-parallel beta-sheet. Unexpectedly, BIs wer...

journal_title：Biological chemistry

authors： Hatano K,Sawano Y,Tanokura M

更新日期：2002-07-01 00:00:00